当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > High-resolution solution structure of the EGF-like domain of heregulin-alpha.

High-resolution solution structure of the EGF-like domain of heregulin-alpha.

Abstract：

:The solution structure of the 63-residue heregulin-alpha (HRG-alpha) epidermal growth factor (EGF)-like domain, corresponding to residues 177-239 of HRG-alpha, has been determined to high resolution using data from two-dimensional and three-dimensional homo- and heteronuclear NMR spectroscopy. The structure is based on a total of 887 internuclear distance and dihedral restraints derived from data obtained using unlabeled and uniformly 15N-labeled protein samples, at pH 4.5, 20 degrees C. A total of 20 structures were calculated using a hybrid distance geometry-simulated annealing approach with the program DGII, followed by restrained molecular dynamics using the program DISCOVER. The average maximum violations are 0.12 +/- 0.01 angstroms and 1.4 +/- 0.3 degrees for distance and dihedral restraints, respectively. The backbone (N,C(alpha),C) atomic rms distribution about the mean coordinates for residues 3-23 and 31-49 is 0.29 +/- 0/07 angstroms. The N-and C-terminal residues (1-2 and 50-63) and 24-30 are disordered. Comparison of the HRG-alpha EGF-like domain structure with the previously determined structure of human EGF [Hommel et al. (1992) J. Mol. Biol. 227, 271-282] reveals a high degree of structural similarity; excluding the N-terminal region (residues 1-13), the disordered phi-loop region (residues 24-30) that contains a three-residue insertion in HRG-alpha relative to hEGF, and the disordered C-terminal region (residues 50-63), the C(alpha) alignment between the HRG-alpha and hEGF minimized mean structures has a rms difference of approximately 1 angstrom. In HRG-alpha the N-terminal residues 2-6 form a well-defined beta strand rather than being disordered as found for hEGF. This structural difference correlates with functional data which suggest that the N-terminal region of the HRG-alpha EGF-like domain is responsible for the observed receptor specificity differences between HRG-alpha and EGF.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Jacobsen NE,Abadi N,Sliwkowski MX,Reilly D,Skelton NJ,Fairbrother WJ

doi

10.1021/bi952626l

subject

Has Abstract

pub_date

1996-03-19 00:00:00

pages

3402-17

issue

11

eissn

0006-2960

issn

1520-4995

pii

bi952626l

journal_volume

35

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Assignment of 15N chemical shifts and 15N relaxation measurements for oxidized and reduced iso-1-cytochrome c.

    abstract：:A protocol for complete isotopic labeling of iso-1-cytochrome c from the eukaryote Saccharomyces cerevisiae is reported. Assignments are reported for the vast majority of the 15N amide resonances in both oxidized and reduced states. 15N heteronuclear relaxation experiments were collected to study the picosecond-nanose...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9827417

    authors： Fetrow JS,Baxter SM

    更新日期：1999-04-06 00:00:00

  • Raman spectroscopy of synthetic antimicrobial frog peptides magainin 2a and PGLa.

    abstract：:Magainin and PGLa are 23- and 21-residue peptides isolated from the skin of the African clawed frog Xenopus laevis. They protect the frog from infection and exhibit a broad-spectrum antimicrobial activity in vitro. The mechanism of this activity involves the interaction of magainin with microbial membranes. We have me...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00470a031

    authors： Williams RW,Starman R,Taylor KM,Gable K,Beeler T,Zasloff M,Covell D

    更新日期：1990-05-08 00:00:00

  • Electrostatic Control of Isoform Selective Inhibitor Binding in Nitric Oxide Synthase.

    abstract：:Development of potent and isoform selective nitric oxide synthase (NOS) inhibitors is challenging because of the structural similarity in the heme active sites. One amino acid difference between NOS isoforms, Asp597 in rat neuronal NOS (nNOS) versus Asn368 in bovine endothelial NOS (eNOS), has been identified as the s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00261

    authors： Li H,Wang HY,Kang S,Silverman RB,Poulos TL

    更新日期：2016-07-05 00:00:00

  • The mechanism of the reaction catalyzed by uronate isomerase illustrates how an isomerase may have evolved from a hydrolase within the amidohydrolase superfamily.

    abstract：:Uronate isomerase (URI) catalyzes the reversible isomerization of D-glucuronate to D-fructuronate and of D-galacturonate to D-tagaturonate. URI is a member of the amidohydrolase superfamily (AHS), a highly divergent group of enzymes that catalyze primarily hydrolytic reactions. The chemical mechanism and active site s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901046x

    authors： Nguyen TT,Fedorov AA,Williams L,Fedorov EV,Li Y,Xu C,Almo SC,Raushel FM

    更新日期：2009-09-22 00:00:00

  • Formaldehyde metabolism by Escherichia coli. In vivo carbon, deuterium, and two-dimensional NMR observations of multiple detoxifying pathways.

    abstract：:13C NMR has been used to demonstrate the metabolism of dilute solutions of labeled formaldehyde by Escherichia coli to methanol, formate, carbon dioxide, and several other unidentified metabolites which contain labeled CH2 groups. Aeration of bacterial suspensions within the spectrometer dramatically increased the rat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00298a017

    authors： Hunter BK,Nicholls KM,Sanders JK

    更新日期：1984-01-31 00:00:00

  • Hydrogen-deuterium exchange and mass spectrometry reveal the pH-dependent conformational changes of diphtheria toxin T domain.

    abstract：:The translocation (T) domain of diphtheria toxin plays a critical role in moving the catalytic domain across the endosomal membrane. Translocation/insertion is triggered by a decrease in pH in the endosome where conformational changes of T domain occur through several kinetic intermediates to yield a final trans-membr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500893y

    authors： Li J,Rodnin MV,Ladokhin AS,Gross ML

    更新日期：2014-11-04 00:00:00

  • Mutation spectra of M13 vectors containing site-specific Cis-Syn, Trans-Syn-I, (6-4), and Dewar pyrimidone photoproducts of thymidylyl-(3'-->5')-thymidine in Escherichia coli under SOS conditions.

    abstract：:The mutations spectra of cis-syn, trans-syn-I, (6-4), and Dewar pyrimidone photoproducts of the TT site of AATTAA and TATTAT in the (-) strand of a heteroduplex M13 vector were obtained in an excision and photoreversal repair deficient Escherichia coli host under SOS conditions. Oligonucleotides containing site-specif...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi951975c

    authors： Smith CA,Wang M,Jiang N,Che L,Zhao X,Taylor JS

    更新日期：1996-04-02 00:00:00

  • The N-terminus of the fragile X mental retardation protein contains a novel domain involved in dimerization and RNA binding.

    abstract：:Fragile X syndrome, the most common cause of inherited mental retardation, is caused by the absence of the fragile X mental retardation protein (FMRP). The emerging picture is that FMRP is involved in repression of translation through a complex network of protein-protein and protein-RNA interactions. Very little struc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034909g

    authors： Adinolfi S,Ramos A,Martin SR,Dal Piaz F,Pucci P,Bardoni B,Mandel JL,Pastore A

    更新日期：2003-09-09 00:00:00

  • Membrane interactions of mutated forms of the influenza fusion peptide.

    abstract：:We have studied a group of fusion peptides of influenza hemagglutinin in which the N-terminal amino acid, Gly (found in the wild-type peptide), has been systematically substituted with Ala, Ser, Val, or Glu. The activity of the intact hemagglutinin protein with these same substitutions has already been reported. As a ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0107187

    authors： Epand RM,Epand RF,Martin I,Ruysschaert JM

    更新日期：2001-07-31 00:00:00

  • Synthesis and biological activity of a lambda pseudo operator.

    abstract：:The chemical and enzymatic syntheses of bacteriophage lambda pseudo operator DNA are described. The 17 base-paired duplex contains the DNA which has been proposed as the binding site for cI repressor protein. The synthetic duplex is twofold symmetric and represents the best possible nucleotide summation of the six pro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00638a013

    authors： Kawashima E,Gadek T,Caruthers MH

    更新日期：1977-09-20 00:00:00

  • Characterization of the fluorophore 4-heptadecyl-7-hydroxycoumarin: a probe for the head-group region of lipid bilayers and biological membranes.

    abstract：:The fluorophore 4-heptadecyl-7-hydroxycoumarin was used as a probe to study the properties of phospholipid bilayers at the lipid-water interface. To this end, the steady-state fluorescence anisotropy, the differential polarized phase fluorometry, and the emission lifetime of the fluorophore were measured in isotropic ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00324a005

    authors： Pal R,Petri WA Jr,Ben-Yashar V,Wagner RR,Barenholz Y

    更新日期：1985-01-29 00:00:00

  • Solution structures of spinach acyl carrier protein with decanoate and stearate.

    abstract：:Acyl carrier protein (ACP) is a cofactor in a variety of biosynthetic pathways, including fatty acid metabolism. Thus, it is of interest to determine structures of physiologically relevant ACP-fatty acid complexes. We report here the NMR solution structures of spinach ACP with decanoate (10:0-ACP) and stearate (18:0-A...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052062d

    authors： Zornetzer GA,Fox BG,Markley JL

    更新日期：2006-04-25 00:00:00

  • Local-access model for proton transfer in bacteriorhodopsin.

    abstract：:The accessibility of the retinal Schiff base in bacteriorhodopsin was studied in the D85N/D96N mutant where the proton acceptor and donor are absent. Protonation and deprotonation of the Schiff base after pH jump without illumination and in the photocycle of the unprotonated Schiff base were measured in the visible an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9728396

    authors： Brown LS,Dioumaev AK,Needleman R,Lanyi JK

    更新日期：1998-03-17 00:00:00

  • Mechanism of action of phalloidin on the polymerization of muscle actin.

    abstract：:Under conditions where muscle actin only partially polymerizes, or where it does not polymerize at all, a significant enhancement of polymerization was observed if equimolar phalloidin was also present. The increased extent of polymerization in the the presence of phalloidin can be explained by the reduced critical ac...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00507a006

    authors： Estes JE,Selden LA,Gershman LC

    更新日期：1981-02-17 00:00:00

  • Cleavage of DNA by the insulin-mimetic compound, NH4[VO(O2)2(phen)].

    abstract：:The kinetics and mechanism of cleavage of DNA by the insulin-mimetic peroxo-vanadate NH4[VO(O2)2(phen)], pV, are described. In the presence of low energy UV radiation or biologically common reducing agents, pV decomposes into the monomer, dimer, and tetramer of vanadate and an uncharacterized compound of V4+ as shown ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9606253

    authors： Hiort C,Goodisman J,Dabrowiak JC

    更新日期：1996-09-24 00:00:00

  • Comparative studies on the 7-iodo and 7-fluoro derivatives of N-acetoxy-N-2-acetylaminofluorene: binding sites on DNA and conformational change of modified deoxytrinucleotides.

    abstract：:GMP and native DNA were reacted with 7-iodo and 7-fluoro derivatives of N-acetoxy-N-2-acetylaminofluorene. It was shown that the 7-halogeno derivatives react on C-8 of guanine. Furthermore the respective amount of arylamidation (covalent linkage on the C-8 of guanine) and arylation (covalent linkage on 2-NH2 groups of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00606a016

    authors： Lefèvre JF,Fuchs RP,Daune MP

    更新日期：1978-06-27 00:00:00

  • Effect of solvent diffusion on the apomyoglobin-water interface.

    abstract：:Few techniques can identify interactions between proteins and individual water molecules when the protein is in solution. The present work has sought to bridge the gap between the molecular level studies and the search for a physical property of the solution (bathing the proteins) that would regulate the protein hydra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi012210+

    authors： Twist C,Royer C,Alpert B

    更新日期：2002-08-13 00:00:00

  • Subunit dissociation as a possible mechanism of glucocorticoid receptor activation.

    abstract：:For the elucidation of the mechanism of steroid hormone receptor activation, the hydrodynamic properties of the unactivated and activated forms of the nonproteolyzed glucocorticoid receptor from the mouse AtT-20 pituitary tumor cell line were determined. The unactivated, molybdate-stabilized receptor has the following...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00277a038

    authors： Vedeckis WV

    更新日期：1983-04-12 00:00:00

  • Cationic copper(II) porphyrins intercalate into domains of double-stranded RNA.

    abstract：:A cationic, copper(II)-containing ligand, derived from bulky 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)porphyrin, Cu(T4), and two sterically friendlier forms, [trans-5,15-di(N-methylpyridinium-4-yl)porphyrinato]copper(II), Cu(tD4), and [cis-5,10-di(N-methylpyridinium-4-yl)porphyrinato]copper(II), Cu(cD4), bind to DN...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300828z

    authors： Briggs BN,Gaier AJ,Fanwick PE,Dogutan DK,McMillin DR

    更新日期：2012-09-25 00:00:00

  • Characterization and kinetic mechanism of catalytic domain of human vascular endothelial growth factor receptor-2 tyrosine kinase (VEGFR2 TK), a key enzyme in angiogenesis.

    abstract：:Vascular endothelial growth factor (VEGF) is a dimeric protein which induces formation of new blood vessels (angiogenesis) through binding to VEGF-receptor-2 tyrosine kinase (VEGFR2 TK) or KDR (kinase insert domain-containing receptor) on the surface of endothelial cells. Angiogenesis has been shown to be essential fo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981291f

    authors： Parast CV,Mroczkowski B,Pinko C,Misialek S,Khambatta G,Appelt K

    更新日期：1998-11-24 00:00:00

  • Solution conformation of the type I collagen alpha-1 chain N-telopeptide studied by 1H NMR spectroscopy.

    abstract：:The solution conformation of the type I collagen alpha-1 chain N-telopeptide has been studied by CD and 1H NMR spectroscopy at 600 MHz in CD3OH/H2O (60/40 v/v) and H2O solutions. The 19 amino acids form the N-terminal end of the alpha-1 polypeptide chain. By the combined application of several two-dimensional, phase-s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00446a006

    authors： Otter A,Kotovych G,Scott PG

    更新日期：1989-10-03 00:00:00

  • Evidence for the involvement of Ala 166 in coupling Na(+) to sugar transport through the human Na(+)/glucose cotransporter.

    abstract：:We mutated residue 166, located in the putative Na(+) transport pathway between transmembrane segments 4 and 5 of human Na(+)/glucose cotransporter (hSGLT1), from alanine to cysteine (A166C). A166C was expressed in Xenopus laevis oocytes, and electrophysiological methods were used to assay function. The affinity for N...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011026l

    authors： Meinild AK,Loo DD,Hirayama BA,Gallardo E,Wright EM

    更新日期：2001-10-02 00:00:00

  • Identification of catalytic residues in the As(III) S-adenosylmethionine methyltransferase.

    abstract：:The enzyme As(III) S-adenosylmethionine methyltransferase (EC 2.1.1.137) (ArsM or AS3MT) is found in members of every kingdom, from bacteria to humans. In these enzymes, there are three conserved cysteine residues at positions 72, 174, and 224 in the CmArsM orthologue from the thermophilic eukaryotic alga Cyanidioschy...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi201500c

    authors： Marapakala K,Qin J,Rosen BP

    更新日期：2012-02-07 00:00:00

  • Comparisons of interfacial Phe, Tyr, and Trp residues as determinants of orientation and dynamics for GWALP transmembrane peptides.

    abstract：:Aromatic amino acids often flank the transmembrane alpha helices of integral membrane proteins. By favoring locations within the membrane-water interface of the lipid bilayer, aromatic residues Trp, Tyr, and sometimes Phe may serve as anchors to help stabilize a transmembrane orientation. In this work, we compare the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500439x

    authors： Sparks KA,Gleason NJ,Gist R,Langston R,Greathouse DV,Koeppe RE 2nd

    更新日期：2014-06-10 00:00:00

  • Nematode Sgt1-homologue D1054.3 binds open and closed conformations of Hsp90 via distinct binding sites.

    abstract：:Heat shock protein 90 (Hsp90) is a highly conserved ATP-driven machine involved in client protein maturation, folding, and activation. The chaperone is supported by a set of cochaperones that confer client specificities. One of those proteins is the suppressor of G2 allele of skp1 (Sgt1), which participates together w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi5000542

    authors： Eckl JM,Drazic A,Rutz DA,Richter K

    更新日期：2014-04-22 00:00:00

  • A structure-based approach to a synthetic vaccine for HIV-1.

    abstract：:The generation of neutralizing antibodies by peptide immunization is dependent on achieving conformational compatibility between antibodies and native protein. Consequently, approaches are needed for developing conformational mimics of protein neutralization sites. We replace putative main-chain hydrogen bonds (NH -->...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0003691

    authors： Cabezas E,Wang M,Parren PW,Stanfield RL,Satterthwait AC

    更新日期：2000-11-28 00:00:00

  • A heterocyclic inhibitor of the REV-RRE complex binds to RRE as a dimer.

    abstract：:As part of a search for organic compounds that selectively target RNA, we found that specific diphenylfuran derivatives, which are related to compounds that bind to the DNA minor groove, bind very strongly to RNA in a manner very sensitive to the structure of the compounds. In extended development of the diphenylfuran...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002338b

    authors： Li K,Davis TM,Bailly C,Kumar A,Boykin DW,Wilson WD

    更新日期：2001-02-06 00:00:00

  • Transient kinetics of redox reactions of flavodoxin: effects of chemical modification of the flavin mononucleotide prosthetic group on the dynamics of intermediate complex formation and electron transfer.

    abstract：:The effects of structural modifications of the flavin mononucleotide (FMN) prosthetic group of Clostridium pasteurianum flavodoxin on the kinetics of electron transfer to the oxidized form (from 5-deazariboflavin semiquinone produced by laser flash photolysis) and from the semiquinone form (to horse heart cytochrome c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00281a034

    authors： Simondsen RP,Tollin G

    更新日期：1983-06-07 00:00:00

  • Interaction of LDS-751 with P-glycoprotein and mapping of the location of the R drug binding site.

    abstract：:One cause of multidrug resistance is the overexpression of P-glycoprotein, a 170 kDa plasma membrane ABC transporter, which functions as an ATP-driven efflux pump with broad specificity for hydrophobic drugs, peptides, and natural products. The protein appears to interact with its substrates within the membrane enviro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0485326

    authors： Lugo MR,Sharom FJ

    更新日期：2005-01-18 00:00:00

  • Nuclear overhauser studies of CCGGAp, ACCGGp, and ACCGGUp.

    abstract：:Nuclear Overhauser effect (NOE) measurements are reported for the nonexchangeable base and ribose 1' proton resonances of CCGGAp, ACCGGp, and ACCGGUp. The results permit assignment of these resonances to particular nucleotides in the sequences. The NOE data rule out conformations containing syn glycosidic linkages and...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00271a005

    authors： Petersheim M,Turner DH

    更新日期：1983-01-18 00:00:00