Abstract：
:Under conditions where muscle actin only partially polymerizes, or where it does not polymerize at all, a significant enhancement of polymerization was observed if equimolar phalloidin was also present. The increased extent of polymerization in the the presence of phalloidin can be explained by the reduced critical actin concentration of partially polymerized populations at equilibrium. Under such conditions, the rate of polymerization, as judged by the length of time to reach half the viscosity plateau, was found to be essentially independent of the phalloidin concentration. Moreover, the initial rate of polymerization of actin was also found to be independent of phalloidin concentration. However, phalloidin apparently causes a reduction in the magnitude of the reverse rates in the polymerization reaction, as was demonstrated by the lack of depolymerization of phalloidin-treated actin polymers. This effect of phalloidin is also supported by the identification of actin nuclei and short polymers in populations of G-actin incubated with phalloidin in the absence of added KCl. Our conclusion, then, is that phalloidin influences the polymerization of actin by stabilizing nuclei and polymers as they are formed.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Estes JE,Selden LA,Gershman LCdoi
10.1021/bi00507a006subject
Has Abstractpub_date
1981-02-17 00:00:00pages
708-12issue
4eissn
0006-2960issn
1520-4995journal_volume
20pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Alpha-synuclein (alphaS) is a lipid-binding synaptic protein of unknown function that is found in an aggregated amyloid fibril form in the intraneuronal Lewy body deposits that are a defining characteristic of Parkinson's disease (PD). Although intrinsically unstructured when free in solution, alphaS adopts a highly h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi036135+
更新日期：2004-04-27 00:00:00
abstract：:A study of the voltage induction of transient pores in phospholipid bilayer vesicles is reported. Unilamellar vesicles (dipalmitoylphosphatidylcholine), with a size distribution of 100 +/- 30 nm, were prepared by the method of Enoch & Strittmatter [Enoch, H., & Strittmatter, P. (1979) Proc. Natl. Acad. Sci. U.S.A. 76,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00509a022
更新日期：1981-03-17 00:00:00
abstract：:Some key chloroplast enzymes are activated by light via a ferredoxin-thioredoxin reduction system which reduces disulfide bridges in the enzymes. We describe for the first time the structural basis for the redox activation of a chloroplast enzyme, the NADP-dependent malate dehydrogenase (MDH) from Sorghum vulgare whos...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982876c
更新日期：1999-04-06 00:00:00
abstract：:The self-association reaction in which four melittin molecules associate to form an aqueously soluble tetramer was studied by fluorescent spectroscopy. At 23 degrees C, pH 7.15, gamma/2 0.50, the dissociation constant, Kd, is 3.20 x 10(-16) M3. At 23 degrees C, gamma/2 0.60, melittin has an amino acyl group with a pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00272a026
更新日期：1983-02-01 00:00:00
abstract：:Protein prenylation involves the attachment of a hydrophobic isoprenoid moiety to the C-terminus of proteins. Several small GTPases, including members of the Ras and Rho subfamilies, require prenylation for their normal and pathological functions. Recent work has suggested that SmgGDS proteins regulate the prenylation...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00567
更新日期：2018-07-24 00:00:00
abstract：:The kinetic mechanism of NADPH-cytochrome P450 oxidoreductase (P450R) has been determined for the model reaction with cytochrome c3+. Although initial velocity studies show parallel patterns, consistent with a classical (one-site) ping-pong mechanism that precludes the formation of a ternary NADPH-P450R-cytochrome c3+...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00206a002
更新日期：1994-10-11 00:00:00
abstract：:5-Hydroxy-1,2-naphthalenedicarboxylic anhydride is closely related to its precursor dibasic acid which is a metabolite of the carcinogenic polynuclear hydrocarbon dibenz[a,h]anthracene. The anhydride inhibited respiration of coupled mitochondria. This inhibition was relieved by 2,4-dinitrophenol. Several mitochondrial...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00695a022
更新日期：1975-12-02 00:00:00
abstract：:Histidine decarboxylase (HDC) from Lactobacillus 30a forms a trimer around a central cavity or well. Three active sites are formed around the well at the interface of each of two adjacent molecules. HDC exhibits cooperative kinetics at pH 7.6 and can be described in terms of a two-state, T and R, model. At pH 4.8, pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00018a009
更新日期：1995-05-09 00:00:00
abstract：:Guanidine hydrochloride (GdnHCl) and thermally induced unfolding measurements on the oxidized form of Escherichia coli thioredoxin at pH 7 were combined for the purpose of assessing the functional dependence of unfolding free energy changes on denaturant concentration over an extended GdnHCl concentration range. Conve...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00135a022
更新日期：1992-05-26 00:00:00
abstract：:Activation of washed human platelets initiated with alpha-thrombin, SFLLRN, or AYPGKF invariably results in the generation of PAR-1-(1-41) and PAR-4-(1-47). PAR-1-(1-41) and PAR-4-(1-47) are amino-terminal peptides generated when PAR-1 and -4 are cleaved in their first extracellular domains after R(41) and R(47), resp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801399c
更新日期：2009-02-24 00:00:00
abstract：:The key polyamine catabolizing enzyme spermidine-spermine N1-acetyltransferase (SSAT) is among the few genes known to be inducible by the natural polyamines. Certain polyamine analogs markedly exaggerate this response and thus provide useful tools for studying the underlying regulatory mechanisms. As shown here, the a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9612273
更新日期：1996-11-12 00:00:00
abstract：:The zinc-endopeptidase light chain of botulinum A neurotoxin undergoes autocatalytic fragmentation that is accelerated by the presence of the metal cofactor, zinc [Ahmed, S. A. et al. (2001) J. Protein Chem. 20, 221-231]. We show in this paper that >95% fragmented light chain obtained in the absence of added zinc reta...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi030062c
更新日期：2003-11-04 00:00:00
abstract：:The crystal structure and spectroscopic properties of the periplasmic penta-heme cytochrome c nitrite reductase (NrfA) of Escherichia coli are presented. The structure is the first for a member of the NrfA subgroup that utilize a soluble penta-heme cytochrome, NrfB, as a redox partner. Comparison to the structures of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015765d
更新日期：2002-03-05 00:00:00
abstract：:1,3-15N-Labeled uracil was synthesized chemically and used to prepare labeled Escherichia coli tRNA(Val) biosynthetically. 500-MHz measurements of 15N and proton chemical shift were obtained, for all uridine and uridine-related bases, by heteronuclear multiple-quantum coherence spectroscopy. All the uracil NH group re...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00166a013
更新日期：1992-12-29 00:00:00
abstract：:β-Barrel membrane proteins are found in the outer membranes of mitochondria, chloroplasts, and Gram-negative bacteria; however, exactly how they are folded and inserted remains unknown. Over the past decade, both functional and structural studies have greatly contributed to addressing this elusive mechanism. It is kno...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.5b00852
更新日期：2015-10-20 00:00:00
abstract：:Treatment of human lymphoid cells with methyl methanesulfonate (MMS) results in discontinuities in cellular DNA. Discontinuities can be detected by sedimentation through neutral sucrose gradients after digestion with S1 nuclease on top of the gradient and by benzoylated naphthoylated diethylaminoethylcellulose (BND-ce...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00639a024
更新日期：1977-10-04 00:00:00
abstract：:14C-Labeled fluorodinitrobenzene and N-ethylmaleimide have been used as chemical probes of the conformational states of myosin induced by the binding of MgADP and MgATP. The results indicate that in the high-energy conformation, MMgADP-Pi, the essential thiols are protected from modification but their diminished react...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00643a005
更新日期：1977-11-29 00:00:00
abstract：:Aromatic residues in c-type cytochromes might have an important function in the folding and/or electron transferring properties of the molecule. In the tetraheme cytochrome c3 (Mr 13 000) from Desulfovibrio vulgaris Hildenborough, Phe20, is located between heme 1 and heme 3 with its aromatic ring close and almost para...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981593h
更新日期：1999-01-05 00:00:00
abstract：:Electron spin polarized electron paramagentic resonance (ESP EPR) spectra were obtained with deuterated iron-removed photosynthetic bacterial reaction centers (RCs) to specifically investigate the effect of the rate of primary charge separation, metal-site occupancy, and H-subunit content on the observed P865+QA- char...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9630319
更新日期：1997-07-15 00:00:00
abstract：:We have studied carbon monoxide (CO) migration and binding in the nonsymbiotic hemoglobins AHb1 and AHb2 of Arabidopsis thaliana using Fourier transform infrared (FTIR) spectroscopy combined with temperature derivative spectroscopy (TDS) at cryogenic temperatures. Both proteins have similar amino acid sequences but di...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100768g
更新日期：2010-09-07 00:00:00
abstract：:Doxorubicin (DOX) is a chemotherapeutic that is used in the treatment of a wide variety of cancers. However, it causes cardiotoxicity partly because of the formation of reactive oxygen species. CYP2J2 is a human cytochrome P450 that is strongly expressed in cardiomyocytes. It converts arachidonic acid (AA) into four d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b01025
更新日期：2017-12-26 00:00:00
abstract：:Calcium and its receptor protein calmodulin function in the regulation of proliferation of mammalian cells. A 68 kDa calmodulin-specific binding protein was shown previously to be associated with growth factor-dependent progression of a variety of mammalian cells from G1 to S phase and to stimulate DNA synthesis in pe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00012a002
更新日期：1995-03-28 00:00:00
abstract：:Two-dimensional (2D) proton magnetic resonance techniques used in conjunction with laser photochemically induced dynamic nuclear polarization (photo-CIDNP) spectroscopy have been applied to studying the kringle 4 domain from human plasminogen at 360 MHz. Out of 11 potential CIDNP-sensitive aromatic side chains, only 5...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00372a020
更新日期：1986-12-02 00:00:00
abstract：:The systems of tRNA glycylation belong to the most complex aminoacylation systems since neither the oligomeric structure of glycyl-tRNA synthetases (GlyRS) nor the discriminator bases in tRNAGly are conserved in the phylae. To better understand the structure-function relationship in glycylation systems of various orig...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991392t
更新日期：1999-10-05 00:00:00
abstract：:Amyloid diseases are caused by the self-assembly of a given protein into an insoluble cross-beta-sheet quaternary structural form which is pathogenic. An understanding of the biochemical mechanism of amyloid fibril formation should prove useful in understanding amyloid disease. Toward this end, a procedure for the con...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00151a036
更新日期：1992-09-15 00:00:00
abstract：:To reduce ribonucleotides to deoxyribonucleotides, the manganese-bound form of class Ib ribonucleotide reductase (RNR) must be activated via a pathway that involves redox protein(s). The reduced flavoprotein NrdI is an important protein in this pathway, as it reduces dioxygen to superoxide. Superoxide then reacts with...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00699
更新日期：2016-09-13 00:00:00
abstract：:Cooperative binding of the bacteriophage lambda cI repressor dimer to specific sites of the phage operators OR and OL controls the developmental state of the phage. Cooperativity has long been thought to be mediated by self-assembly of repressor dimers to form tetramers which can bind simultaneously to adjacent operat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971368k
更新日期：1997-10-21 00:00:00
abstract：:A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense. We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00614
更新日期：2020-09-29 00:00:00
abstract：:Human liver alanine aminopeptidase is inhibited by L-amino acids having hydrophobic side chains such as Phe, Tyr, Trp, Met, and Leu. Blocking of the amino group or the carboxyl group greatly reduces the inhibitory capacity of the amino acid. Kinetic studies demonstrate that inhibition of hydrolysis of the substrate L-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00685a027
更新日期：1975-07-15 00:00:00
abstract：:Secretory carrier membrane protein 2 (SCAMP2) functions in late steps of membrane fusion in calcium-dependent granule exocytosis. A basic/hydrophobic peptide segment within SCAMP2 (SCAMP2 E: CWYRPIYKAFR) has been implicated in this function and shown to bind and sequester phosphatidylinositol 4,5-bisphosphate [PI(4,5)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701121j
更新日期：2007-09-25 00:00:00
