Spin-label electron paramagnetic resonance and differential scanning calorimetry studies of the interaction between mitochondrial cytochrome c oxidase and adenosine triphosphate synthase complex.

abstract：:The complex of ribonuclease A (RNase A) with uridine vanadate (U-V), a transition-state analogue, has been studied with 51V and proton NMR spectroscopy in solution and by neutron diffraction in the crystalline state. Upon the addition of aliquots of U-V at pH 6.6, the C epsilon-H resonances of the two active-site hist...

journal_title：Biochemistry

authors： Borah B,Chen CW,Egan W,Miller M,Wlodawer A,Cohen JS

更新日期：1985-04-09 00:00:00

abstract：:The members of the glutamine amidotransferase (GATase) family catalyze the incorporation of ammonia within numerous metabolic pathways and can be categorized in two classes. Here, we concentrated on class I GATases, which are heteromeric enzyme complexes consisting of synthase subunits and glutaminase subunits with a ...

journal_title：Biochemistry

authors： Semmelmann F,Hupfeld E,Heizinger L,Merkl R,Sterner R

更新日期：2019-06-04 00:00:00

abstract：:The common reactions of dioxygen, superoxide, and hydroperoxides with thiolates are thought to proceed via persulfenate intermediates, yet these have never been visualized. Here we report a 1.4 A resolution crystal structure of the Fe(2+)-dependent enzyme cysteine dioxygenase (CDO) containing this putative intermediat...

journal_title：Biochemistry

authors： Simmons CR,Krishnamoorthy K,Granett SL,Schuller DJ,Dominy JE Jr,Begley TP,Stipanuk MH,Karplus PA

更新日期：2008-11-04 00:00:00

abstract：:The serotonin transporter (SERT) is a member of a highly homologous family of proteins responsible for the reuptake of biogenic amines from the synaptic cleft. We took advantage of native restriction sites in SERT to construct a chimeric transporter containing a small (34 amino acid) region of the norepinephrine trans...

journal_title：Biochemistry

authors： Stephan MM,Chen MA,Penado KM,Rudnick G

更新日期：1997-02-11 00:00:00

abstract：:Native calf thymus DNA was reacted with N-acetoxy-N-2-acetylaminofluorene (N-AcO-AAF) and its 7-fluoro and 7-iodo derivatives. Different ways of purification of the fluorene modified DNA samples were checked in order ot obtain a nucleic acid free from all noncovalently bound fluorene residues. The decrease in melting ...

journal_title：Biochemistry

authors： Fuchs RP,Lefevre JF,Pouyet J,Daune MP

更新日期：1976-07-27 00:00:00

abstract：:PACT is a stress-modulated activator of interferon (IFN)-induced double-stranded (ds) RNA-activated protein kinase (PKR) and is an important regulator of PKR-dependent signaling pathways. Stress-induced phosphorylation of PACT is essential for PACT's association with PKR leading to PKR activation. PKR activation by PA...

journal_title：Biochemistry

authors： Singh M,Castillo D,Patel CV,Patel RC

更新日期：2011-05-31 00:00:00

abstract：:Mutagenized dockerin domains of endoglucanase CelD (type I) and of the cellulosome-integrating protein CipA (type II) were constructed by swapping residues 10 and 11 of the first or the second duplicated segment between the two polypeptides. These residues have been proposed to determine the specificity of cohesin-doc...

journal_title：Biochemistry

authors： Schaeffer F,Matuschek M,Guglielmi G,Miras I,Alzari PM,Béguin P

更新日期：2002-02-19 00:00:00

abstract：:The type III secretion system (T3SS) is an essential virulence factor for Shigella flexneri , providing a conduit through which host-altering effectors are injected directly into a host cell to promote uptake. The type III secretion apparatus (T3SA) is composed of a basal body, external needle, and regulatory tip comp...

journal_title：Biochemistry

authors： Dickenson NE,Arizmendi O,Patil MK,Toth RT 4th,Middaugh CR,Picking WD,Picking WL

更新日期：2013-12-10 00:00:00

abstract：:Cdc42 and Rac are highly homologous members of the Rho family of small G proteins that interact with several downstream effector proteins thereby causing cytoskeletal rearrangements, cell proliferation, and differentiation. While some effectors, such as the tyrosine kinase, ACK, and the scaffold protein, WASP, are uni...

journal_title：Biochemistry

authors： Elliot-Smith AE,Mott HR,Lowe PN,Laue ED,Owen D

更新日期：2005-09-20 00:00:00

abstract：:Ca2+-activated Z-disk-removing activity in the P0-40 crude muscle extracts described by Busch et al. (Busch, W. A., Stromer, M. H., Goll, D. E., and Suzuki, A. (1972), J. Cell Biol. 52, 367) was purified from porcine skeletal muscle extracts by using five column chromatographic procedures in succession: (1) 6% agarose...

journal_title：Biochemistry

authors： Dayton WR,Goll DE,Zeece MG,Robson RM,Reville WJ

更新日期：1976-05-18 00:00:00

abstract：:The solution conformation of potato carboxypeptidase inhibitor (CPI) has been investigated by 1H NMR spectroscopy. The spectrum is assigned in a sequential manner by using two-dimensional NMR techniques to identify through-bond and through-space (less than 5 A) connectivities. A set of 309 approximate interproton dist...

journal_title：Biochemistry

authors： Clore GM,Gronenborn AM,Nilges M,Ryan CA

更新日期：1987-12-01 00:00:00

abstract：:Reconstitution of highly purified aquaporin CHIP (channel-forming integral protein) into proteoliposomes was previously shown to confer high osmotic water permeability (Pf) to the membranes [Zeidel et al. (1992) Biochemistry 31, 7436-7440]. Here we report detailed ultrastructural, pharmacologic, and transport studies ...

journal_title：Biochemistry

authors： Zeidel ML,Nielsen S,Smith BL,Ambudkar SV,Maunsbach AB,Agre P

更新日期：1994-02-15 00:00:00

abstract：:The interaction between eosin-5-maleimide (EMA), an inhibitor of the anion transport protein, band 3, and I-, a transportable substrate, was investigated by fluorescence quenching. The Stern-Volmer plot for the quenching reaction between EMA-labeled band 3 and I- exhibits downward curvature both in human erythrocyte g...

journal_title：Biochemistry

authors： Pan RJ,Cherry RJ

更新日期：1995-04-11 00:00:00

abstract：:We have isolated from rabbit liver three cDNA clones of 1400-1800 base pairs that hybridize selectively to RNA from animals treated with phenobarbital. The nucleotide sequences of the cDNAs have been determined. In the protein coding region the nucleotide sequences of two of the cDNAs are 88% homologous, and the third...

journal_title：Biochemistry

authors： Leighton JK,DeBrunner-Vossbrinck BA,Kemper B

更新日期：1984-01-17 00:00:00

abstract：:Previous studies of P-glycoprotein have demonstrated that its function can be modulated by phosphorylation. In the present study, inhibition of protein kinase C with calphostin C or stauroporine or prolonged treatment with the phorbol ester TPA decreased phosphorylation of P-glycoprotein, and impaired transport of vin...

journal_title：Biochemistry

authors： Bates SE,Lee JS,Dickstein B,Spolyar M,Fojo AT

更新日期：1993-09-07 00:00:00

abstract：:Thioredoxin reductase from Escherichia coli is a member of the pyridine nucleotide-disulfide oxidoreductase family, and contains one FAD and one redox-active disulfide per subunit. It is known that two other well-studied members of this family, lipoamide dehydrogenase and glutathione reductase, cycle between the two e...

journal_title：Biochemistry

authors： Lennon BW,Williams CH Jr

更新日期：1996-04-16 00:00:00

abstract：:CDP-ribitol synthase is a bifunctional reductase and cytidylyltransferase that catalyzes the transformation of D-ribulose 5-phosphate, NADPH, and CTP to CDP-ribitol, a repeating unit present in the virulence-associated polysaccharide capsules of Haemophilus influenzae types a and b [Follens, A., et al. (1999) J. Bacte...

journal_title：Biochemistry

authors： Zolli M,Kobric DJ,Brown ED

更新日期：2001-04-24 00:00:00

abstract：:It has previously been shown that 9S, untransformed progestin, estrogen, androgen, and glucocorticoid receptor complexes in rabbit uterine and liver cytosols contain a 59-kDa protein [Tai, P. K., Maeda, Y., Nakao, K., Wakim, N. G., Duhring, J. L., & Faber, L. E. (1986) Biochemistry 25, 5269-5275]. In this work we show...

journal_title：Biochemistry

authors： Sanchez ER,Faber LE,Henzel WJ,Pratt WB

更新日期：1990-05-29 00:00:00

abstract：:Serine palmitoyltransferase (EC 2.3.1.50) catalyzes the condensation of L-serine and palmitoyl-CoA to yield 3-ketosphinganine in the first unique reaction of long-chain (sphingoid) base biosynthesis. The kinetic effects of changing the extracellular concentrations of the precursors for this pathway were studied with L...

journal_title：Biochemistry

authors： Merrill AH Jr,Wang E,Mullins RE

更新日期：1988-01-12 00:00:00

abstract：:Eukaryotic translation initiation factor 2B (eIF2B) is the guanine nucleotide exchange factor of the GTPase eIF2, which brings the initiator Met-tRNAi to the ribosome in the form of the eIF2-GTP·Met-tRNAi ternary complex (TC). The activity of eIF2B is inhibited by phosphorylation of its substrate eIF2 by several stres...

journal_title：Biochemistry

authors： Bogorad AM,Lin KY,Marintchev A

更新日期：2018-03-06 00:00:00

abstract：:RecA-catalyzed DNA recombination is initiated by a mandatory, high-energy form of DNA in RecA-nucleoprotein filaments, where bases are highly unstacked and the backbone is highly unwound. Interestingly, only the energetics consequent to adenosine triphosphate (ATP) binding, rather than its hydrolysis, seems sufficient...

journal_title：Biochemistry

authors： Ramreddy T,Sen S,Rao BJ,Krishnamoorthy G

更新日期：2003-10-21 00:00:00

abstract：:Factor XIII is the terminal enzyme of the clotting cascade. A cDNA sequence encoding human placental factor XIII was expressed in Saccharomyces cerevisiae with the yeast ADH2-4c promoter. Expression levels were a strong function of the noncoding flanking DNA content of the construction. When the terminal 3'-flanking n...

journal_title：Biochemistry

authors： Bishop PD,Teller DC,Smith RA,Lasser GW,Gilbert T,Seale RL

更新日期：1990-02-20 00:00:00

abstract：:The free solution electrophoretic mobilities and thermal stabilities of hairpins formed by two complementary 26-nucleotide oligomers have been measured by capillary electrophoresis. The oligomers are predicted to form molecular beacon-like hairpins with 5 bp stems and 16 nucleotides in the loop. One hairpin, called ha...

journal_title：Biochemistry

authors： Chang CY,Stellwagen NC

更新日期：2011-10-25 00:00:00

abstract：:The 1,2-diacyl-sn-glycerols (1,2-DGs) are the predominant naturally occurring isomer found in cell membranes, lipid droplets, and lipoproteins. They are involved in the metabolism of monoacylglycerols, triacylglycerols, and phospholipids. The 1,2-DGs participate in the activation of protein kinase C, in phosphorylatio...

journal_title：Biochemistry

authors： Kodali DR,Fahey DA,Small DM

更新日期：1990-12-04 00:00:00

abstract：:Prion diseases are associated with the conversion of the normal prion protein, PrP(C), to the infectious disease form PrP(Sc). Discrimination between these isoforms would significantly enhance diagnosis of these diseases, and it has recently been reported that PrP(Sc) is specifically recognized by the serine protease ...

journal_title：Biochemistry

authors： Ellis V,Daniels M,Misra R,Brown DR

更新日期：2002-06-04 00:00:00

abstract：:Introduction of specific structural probes into substrate binding sites of Escherichia coli glutamine synthetase is now possible. Various analogues of ATP substituted with an amino or sulfhydryl moiety at the 6- or 8-position of the purine ring have been found to substitute for ATP in the autoinactivation reaction of ...

journal_title：Biochemistry

authors： Maurizi MR,Ginsburg A

更新日期：1986-01-14 00:00:00

abstract：:Attempts to alter the guanine specificity of ribonuclease T1 (RNase T1) by rational or random mutagenesis have failed so far. The RNase T1 variant RV (Lys41Glu, Tyr42Phe, Asn43Arg, Tyr45Trp, and Glu46Asn) designed by combination of a random and a rational mutagenesis approach, however, exhibits a stronger preference t...

journal_title：Biochemistry

authors： Czaja R,Struhalla M,Höschler K,Saenger W,Sträter N,Hahn U

更新日期：2004-03-16 00:00:00

abstract：:The coordination complex tris(4,7-diphenyl-1,10-phenanthroline)rhodium(III) [Rh(DIP)3(3+)], which promotes RNA cleavage upon photoactivation, has been shown to target specifically guanine-uracil (G-U) mismatches in double-helical regions of folded RNAs. Photoactivated cleavage by Rh(DIP)3(3+) has been examined on a se...

journal_title：Biochemistry

authors： Chow CS,Barton JK

更新日期：1992-06-23 00:00:00

abstract：:Synaptotagmin I is the calcium sensor in synchronous neurotransmitter release caused by fusion of synaptic vesicles with the presynaptic membrane in neurons. Synaptotagmin I interacts with acidic phospholipids, but also with soluble N-ethylmaleimide-sensitive factor attachment receptors (SNAREs), at various stages in ...

journal_title：Biochemistry

authors： Wan C,Kiessling V,Cafiso DS,Tamm LK

更新日期：2011-04-05 00:00:00

abstract：:Coiled coils (CCs) are among the best-understood protein folds. Nonetheless, there are gaps in our knowledge of CCs. Notably, CCs are likely to be structurally more dynamic than often considered. Here, we explore this in an abundant class of CCs, parallel dimers, focusing on polar asparagine (Asn) residues in the hydr...

journal_title：Biochemistry

authors： Thomas F,Niitsu A,Oregioni A,Bartlett GJ,Woolfson DN

更新日期：2017-12-19 00:00:00