A putative Fe2+-bound persulfenate intermediate in cysteine dioxygenase.

abstract：:Stabilization of the plasma membrane Ca-ATPase (PMCA) in an inactive conformation upon oxidation of multiple methionines in the calcium regulatory protein calmodulin (CaM) is part of an adaptive cellular response to minimize ATP utilization and the generation of reactive oxygen species (ROS) under conditions of oxidat...

journal_title：Biochemistry

authors： Chen B,Mayer MU,Squier TC

更新日期：2005-03-29 00:00:00

abstract：:The field of chemical site-selective modification of proteins has progressed extensively in recent decades to enable protein functionalization for imaging, drug delivery, and functional studies. In this Perspective, we provide detailed insight into an alternative use of site-selective protein chemistry to probe the ro...

journal_title：Biochemistry

authors： Bertoldo JB,Terenzi H,Hüttelmaier S,Bernardes GJL

更新日期：2018-10-30 00:00:00

abstract：:The three-dimensional structure of amyloid beta peptide (25-35), which has neurotoxic activity, in lithium dodecyl sulfate micelles was determined by two-dimensional 1H NMR spectroscopy with simulated annealing calculations. A total of 20 converged amyloid beta peptide structures were obtained on the basis of 110 expe...

journal_title：Biochemistry

authors： Kohno T,Kobayashi K,Maeda T,Sato K,Takashima A

更新日期：1996-12-17 00:00:00

abstract：:Maltosyl isothiocyanate (MITC) has been synthesized from maltose with an overall yield of 88%. It has been found to be a potent irreversible inhibitor of zero trans influx of glucose with human erythrocytes. Kinetic analysis of glucose transport after treatment of erythrocytes with MITC revealed that VT was diminished...

journal_title：Biochemistry

authors： Mullins RE,Langdon RG

更新日期：1980-03-18 00:00:00

abstract：:Arginine-70 of human dihydrofolate reductase (hDHFR) is a highly conserved residue which X-ray crystallographic data have shown to interact with the alpha-carboxylate of the terminal L-glutamate moiety of either folic acid or methotrexate (MTX). The rationale for this study was to introduce a conservative amino acid r...

journal_title：Biochemistry

authors： Thompson PD,Freisheim JH

更新日期：1991-08-20 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) allows the study of interactions of fluorescently labeled ligand with receptors in living cells at single-molecule detection sensitivity. From the autocorrelation functions of fluorescence intensity fluctuations, the diffusion time of molecules through the confocal volume is...

journal_title：Biochemistry

authors： Pramanik A,Olsson M,Langel U,Bartfai T,Rigler R

更新日期：2001-09-11 00:00:00

abstract：:Intrastrand cross-links (IaCL) connecting two purine nucleobases in DNA pose a challenge to high-fidelity replication in the cell. Various repair pathways or polymerase bypass can cope with these lesions. The influence of the phosphodiester linkage between two neighboring 2'-deoxyguanosine (dG) residues attached throu...

journal_title：Biochemistry

authors： O'Flaherty DK,Guengerich FP,Egli M,Wilds CJ

更新日期：2015-12-29 00:00:00

abstract：:Calmodulin (CaM) is an essential, eukaryotic protein comprised of two highly homologous domains (N and C). CaM binds four calcium ions cooperatively, regulating a wide array of target proteins. A genetic screen of Paramecia by Kung [Kung, C. et al. (1992) Cell Calcium 13, 413-425] demonstrated that the domains of CaM ...

journal_title：Biochemistry

authors： VanScyoc WS,Newman RA,Sorensen BR,Shea MA

更新日期：2006-12-05 00:00:00

abstract：:The interaction between the calcium binding and inhibitory components of troponin is central to the regulation of muscle contraction. In this work, two-dimensional heteronuclear single-quantum coherence nuclear magnetic resonance (2D-¿1H,15N¿-HSQC NMR) spectroscopy was used to determine the stoichiometry, affinity, an...

journal_title：Biochemistry

authors： Mercier P,Li MX,Sykes BD

更新日期：2000-03-21 00:00:00

abstract：:Protein L is a multidomain protein expressed at the surface of some strains of the anaerobic bacterial species Peptostreptococcus magnus. It has affinity for immunoglobulin (Ig) through interaction with framework structures in the variable Ig light chain domain. The Ig-binding activity is located to five homologous re...

journal_title：Biochemistry

authors： Wikström M,Drakenberg T,Forsén S,Sjöbring U,Björck L

更新日期：1994-11-29 00:00:00

abstract：:Binding of urokinase-type plasminogen activator (uPA) to its cellular receptor (uPAR) renders the cell surface a favored site for plasminogen activation. Recently, a 15-mer peptide antagonist of the uPA-uPAR interaction, with an IC50 value of 10 nM, was identified using phage display technology [Goodson, R. J., Doyle,...

journal_title：Biochemistry

authors： Ploug M,Ostergaard S,Hansen LB,Holm A,Danø K

更新日期：1998-03-17 00:00:00

abstract：:Synthetic O-glycopeptides containing one or two GalNAc residues attached to Ser or Thr were used as substrates to investigate the effect of peptide structure on the activity of crude preparations of UDP-Gal:GalNAc alpha-R beta 3-Gal-transferase from pig stomach and pig and rat colonic mucosa and of a partially purifie...

journal_title：Biochemistry

authors： Brockhausen I,Möller G,Merz G,Adermann K,Paulsen H

更新日期：1990-11-06 00:00:00

abstract：:Oxalate is the strongest known inhibitor of yeast cytochrome b2 activity. We have used spectrophotometric titration, temperature-jump relaxation, and calorimetry in an investigation of the interaction between enzyme and inhibitor. The titration data are consistent with noncooperative binding to one site per subunit. T...

journal_title：Biochemistry

authors： Thusius D,Blazy B,Baudras A

更新日期：1976-01-27 00:00:00

abstract：:Although tryptophan synthase catalyzes a number of pyridoxal phosphate dependent beta-elimination and beta-replacement reactions that are also catalyzed by tryptophanase, a principal and puzzling difference between the two enzymes lies in the apparent inability of tryptophan synthase to catalyze beta-elimination of in...

journal_title：Biochemistry

authors： Ahmed SA,Martin B,Miles EW

更新日期：1986-07-29 00:00:00

abstract：:ROS-GC represents a membrane guanylate cyclase subfamily whose distinctive feature is that it transduces diverse intracellularly generated Ca(2+) signals into the production of the second messenger cyclic GMP. An intriguing feature of the first subfamily member, ROS-GC1, is that it is both stimulated and inhibited by ...

journal_title：Biochemistry

authors： Kumar VD,Vijay-Kumar S,Krishnan A,Duda T,Sharma RK

更新日期：1999-09-28 00:00:00

abstract：:We previously showed that a bulky ring-strained cycloalkyne possessing a rhodamine fluorophore directly reacts (via strain-promoted click chemistry) with the azido group incorporated (via ligand-directed tosyl chemistry) into Asp160 in the 49 kDa subunit of complex I in bovine heart submitochondrial particles [Masuya,...

journal_title：Biochemistry

authors： Masuya T,Murai M,Ito T,Aburaya S,Aoki W,Miyoshi H

更新日期：2017-08-15 00:00:00

abstract：:It has previously been shown that mammalian RNA-peptidyl complexes are found in close association with tRNA, but can be separated from the bulk of the tRNA by benzoylated diethylaminoethylcellulose chromatography (Kull, F.J., and Soodak, M. (1971), Biochim. Biophys. Acta 246, l; Gadski, R.A., and Kull, F.J. (1973), Bi...

journal_title：Biochemistry

authors： Fisher PA,Goodman JG,Kull FJ

更新日期：1976-09-07 00:00:00

abstract：:In search of the regulation mechanisms for isoform specific myosin assembly, we have used the COOH-terminal fragments of nonmuscle myosin isoforms MIIA and MIIB (MIIA(F46) and MIIB(alpha)(F47)) as a model system. Phosphorylation by protein kinase C (PK C) or casein kinase II (CK II) within or near the nonhelical tail-...

journal_title：Biochemistry

authors： Murakami N,Kotula L,Hwang YW

更新日期：2000-09-19 00:00:00

abstract：:The enzyme steroid 5 alpha-reductase utilizes NADPH to reduce the double bonds of a variety of steroid substrates with generalized 3-oxo, delta 4,5 structures. One substrate for this membrane-bound enzyme is testosterone, whose reduction to dihydrotestosterone is required for the embryonic differentiation of the exter...

journal_title：Biochemistry

authors： Wigley WC,Prihoda JS,Mowszowicz I,Mendonca BB,New MI,Wilson JD,Russell DW

更新日期：1994-02-08 00:00:00

abstract：:Protein methylation is a common post-translational modification with diverse biological functions. Methyllysine reader proteins are increasingly a focus of epigenetics research and play important roles in regulating many cellular processes. These reader proteins are vital players in development, cell cycle regulation,...

journal_title：Biochemistry

authors： Milosevich N,Hof F

更新日期：2016-03-22 00:00:00

abstract：:The aspartate receptor is one of the ligand-specific, homodimeric chemoreceptors that detects extracellular attractants and triggers the chemotaxis pathway of Escherichia coli and Salmonella typhimurium. This receptor regulates the activity of the histidine kinase CheA, which forms a kinetically stable complex with th...

journal_title：Biochemistry

authors： Winston SE,Mehan R,Falke JJ

更新日期：2005-09-27 00:00:00

abstract：:The catalytic activity and inhibitor binding energetics of enzymes are often pH-dependent properties. Aspartic proteases comprise an important class of enzyme targets for structure-based drug design. We have performed a complete thermodynamic study of pepstatin binding to plasmepsin II, an aspartic proteinase found in...

journal_title：Biochemistry

authors： Xie D,Gulnik S,Collins L,Gustchina E,Suvorov L,Erickson JW

更新日期：1997-12-23 00:00:00

abstract：:The data and approach reported in paper I (Hanlon et al., 1975, preceding paper) have been used to calculate the fractional changes in secondary structure of calf thymus deoxyribonucleic acid which occur in aqueous solutions as a function of the concentration of NaCl, KCl, LiCl, CsCl, and NH4Cl. There is a continuous ...

journal_title：Biochemistry

authors： Wolf B,Hanlon S

更新日期：1975-04-22 00:00:00

abstract：:The hypothesis that the stable ternary complex formed between myosin subfragment-1, MgADP and beryllium fluoride (BeF3-), denoted S-1 not equal to .ADP.BeF3-, is an analog of the intermediate state S-1**.ADP.P(i) has been tested in this work by examining the interactions of S-1 not equal to .ADP.BeF3- with actin. Equi...

journal_title：Biochemistry

authors： Phan BC,Faller LD,Reisler E

更新日期：1993-08-03 00:00:00

abstract：:The egg vitelline envelope of the marine invertebrate, Megathura crenulata, was lyzed either by sperm lysins A, B, C or by dithiothreitol. In each case the lysis mixture consisted of two major fractions, I and II, that could be separated by hydroxylapatite chromatography and had different electrophoretic mobilities on...

journal_title：Biochemistry

authors： Heller E,Raftery MA

更新日期：1976-03-23 00:00:00

abstract：:The importance of the interdomain bridging interactions observed only in the R-state structure of Escherichia coli aspartate transcarbamylase between Glu-50 of the carbamoyl phosphate domain with both Arg-167 and Arg-234 of the aspartate domain has been investigated by using site-specific mutagenesis. Two mutant versi...

journal_title：Biochemistry

authors： Newton CJ,Kantrowitz ER

更新日期：1990-02-13 00:00:00

abstract：:Quinolinate phosphoribosyltransferase (QAPRTase, EC 2.4.2.19) catalyzes the formation of nicotinate mononucleotide, carbon dioxide, and pyrophosphate from 5-phosphoribosyl 1-pyrophosphate (PRPP) and quinolinic acid (QA, pyridine 2,3-dicarboxylic acid). The enzyme is the only type II PRTase whose X-ray structure is kno...

journal_title：Biochemistry

authors： Cao H,Pietrak BL,Grubmeyer C

更新日期：2002-03-12 00:00:00

abstract：:Two-dimensional (2D) proton magnetic resonance techniques used in conjunction with laser photochemically induced dynamic nuclear polarization (photo-CIDNP) spectroscopy have been applied to studying the kringle 4 domain from human plasminogen at 360 MHz. Out of 11 potential CIDNP-sensitive aromatic side chains, only 5...

journal_title：Biochemistry

authors： De Marco A,Zetta L,Petros AM,Llinás M,Boelens R,Kaptein R

更新日期：1986-12-02 00:00:00

abstract：:During T cell signaling, Itk selectively phosphorylates a tyrosine within its own SH3 domain and a tyrosine within PLCgamma1. We find that the remote SH2 domain in each of these substrates is required to achieve efficient tyrosine phosphorylation by Itk and extend this observation to two other Tec family kinases, Btk ...

journal_title：Biochemistry

authors： Joseph RE,Min L,Xu R,Musselman ED,Andreotti AH

更新日期：2007-05-08 00:00:00

abstract：:Calmodulin (CaM), by mediating the stimulation of the activity of two adenylyl cyclases (ACs), plays a key role in integrating the cAMP and Ca(2+) signaling systems. These ACs, AC1 and AC8, by decoding discrete Ca(2+) signals can contribute to fine-tuning intracellular cAMP dynamics, particularly in neurons where they...

journal_title：Biochemistry

authors： Masada N,Schaks S,Jackson SE,Sinz A,Cooper DM

更新日期：2012-10-09 00:00:00