A putative Fe2+-bound persulfenate intermediate in cysteine dioxygenase.

abstract：:5-HT(2A) serotonin receptors represent the principal molecular targets for LSD-like hallucinogens and atypical antipsychotic drugs. It has been proposed that a dysregulation of 5-HT(2A) receptor-mediated signaling may contribute to the pathogenesis of schizophrenia and related diseases. A major mechanism for the atten...

journal_title：Biochemistry

authors： Gray JA,Compton-Toth BA,Roth BL

更新日期：2003-09-16 00:00:00

abstract：:The bacterial reaction center (RC) converts light into chemical energy through the reduction of an internal quinone molecule Q(B) to Q(B)H(2). In the native RC, proton transfer is coupled to electron transfer and is not rate-controlling. Consequently, proton transfer is not directly observable, and its rate was unknow...

journal_title：Biochemistry

authors： Paddock ML,Adelroth P,Feher G,Okamura MY,Beatty JT

更新日期：2002-12-17 00:00:00

abstract：:IIIGlc is an 18.1-kDa signal-transducing phosphocarrier protein of the phosphoenolpyruvate:glycose phosphotransferase system (PTS) of Escherichia coli. Virtually complete (98%) backbone 1H, 15N, and 13C nuclear magnetic resonance (NMR) signal assignments were determined by using a battery of triple-resonance three-dim...

journal_title：Biochemistry

authors： Pelton JG,Torchia DA,Meadow ND,Wong CY,Roseman S

更新日期：1991-10-15 00:00:00

abstract：:The thermotropic behavior of aqueous dispersions of stearoylsphingomyelin-cholesterol mixtures was examined by high-sensitivity differential scanning calorimetry. When less than 20 mol % cholesterol was mixed with the sphingomyelin and the samples were held at room temperature for 7-9 days before the initiation of cal...

journal_title：Biochemistry

authors： Estep TN,Freire E,Anthony F,Barenholz Y,Biltonen RL,Thompson TE

更新日期：1981-12-08 00:00:00

abstract：:Isolated alpha and beta chains from Xenopus laevis hemoglobin have been purified. The isolation procedure yields native alpha chains whose functional behavior has been characterized and compared with that of human alpha chains. Isolated beta chains in the presence of oxygen are characterized by low stability, and henc...

journal_title：Biochemistry

authors： Condò SG,Giardina B,Bellelli A,Brunori M

更新日期：1987-10-20 00:00:00

abstract：:Sensory rhodopsin I (SRI) is a seven-transmembrane helix retinylidene protein that mediates color-sensitive phototaxis responses through its bound transducer HtrI in the archaeon Halobacterium salinarum. Deprotonation of the Schiff base attachment site of the chromophore accompanies formation of the SRI signaling stat...

journal_title：Biochemistry

authors： Jung KH,Spudich EN,Dag P,Spudich JL

更新日期：1999-10-05 00:00:00

abstract：:Sonication of lysophosphatidylcholine (lysoPC; 20 mumol/mL) and cholesterol (chol) in aqueous medium produces lamellar structures over a wide range of concentrations. From 25 to 47 mol % cholesterol, electron microscopy (EM) after negative staining showed extended stacklike lamellae about 40 A thick. From 50 to 60 mol...

journal_title：Biochemistry

authors： Kumar VV,Anderson WH,Thompson EW,Malewicz B,Baumann WJ

更新日期：1988-01-12 00:00:00

abstract：:Cdx and Hox gene families encode homeodomain-containing transcription factors involved in anterior-posterior vertebral patterning. Although Cdx proteins are direct transcriptional regulators of Hox gene expression, both Hox and Cdx proteins are known to interact with other homeodomain transcription factors, leading us...

journal_title：Biochemistry

authors： Lafontaine CA,Grainger S,Hess BL,Béland M,Lohnes D

更新日期：2012-12-04 00:00:00

abstract：:Internal fatty acylation of proteins is a recognized means of modifying biological behavior. Escherichia coli hemolysin A (HlyA), a toxic protein, is transcribed as a nontoxic protein and made toxic by internal acylation of two lysine residue epsilon-amino groups; HlyC catalyzes the acyl transfer from acyl-acyl carrie...

journal_title：Biochemistry

authors： Trent MS,Worsham LM,Ernst-Fonberg ML

更新日期：1999-07-06 00:00:00

abstract：:The differential scanning calorimetry trace of F1ATPase, prepared from beef heart submitochondrial particles, has a single sharp endothermic transition at 80.5 +/- 1.0 degrees C and a half-height peak width of 2.0 +/- 0.2 degrees. The transition enthalpy is 19 +/- 2 cal/g of protein. Submitochondrial particles (SMP) h...

journal_title：Biochemistry

authors： Kresheck GC,Adade AB,Vanderkooi G

更新日期：1985-03-26 00:00:00

abstract：:Type IV collagen promotes integrin-mediated cell adhesion, spreading, and motility. Several regions within the triple-helical domain of type IV collagen have been identified as tumor cellular recognition sites. Among these regions, the alpha1(IV)531-543 sequence, designated L-Hep-III, promotes integrin-mediated tumor ...

journal_title：Biochemistry

authors： Li C,McCarthy JB,Furcht LT,Fields GB

更新日期：1997-12-09 00:00:00

abstract：:Previous studies have shown that murine macrophages immunostimulated with interferon gamma and Escherichia coli lipopolysaccharide synthesize NO2-, NO3-, and citrulline from L-arginine by oxidation of one of the two chemically equivalent guanido nitrogens. The enzymatic activity for this very unusual reaction was foun...

journal_title：Biochemistry

authors： Marletta MA,Yoon PS,Iyengar R,Leaf CD,Wishnok JS

更新日期：1988-11-29 00:00:00

abstract：:Piscidin 1 (Pis-1) is a novel cytotoxic peptide with a cationic alpha-helical structure that was isolated from the mast cells of hybrid striped bass [Silphaduang, U., and Noga, E. J. (2001) Nature 414, 268-269]. Pis-1 is not selective for bacterial versus mammalian cells. In the present study, to develop novel antibio...

journal_title：Biochemistry

authors： Lee SA,Kim YK,Lim SS,Zhu WL,Ko H,Shin SY,Hahm KS,Kim Y

更新日期：2007-03-27 00:00:00

abstract：:The major covalently linked multimolecular D fragments found in plasmic digests of factor XIIIa cross-linked fibrin formed under physiological pH and ionic strength conditions consist of D dimers, D trimers, and D tetramers. These fragments are linked by epsilon-amino-gamma-glutamyllysine bonds in the carboxy-terminal...

journal_title：Biochemistry

authors： Siebenlist KR,Mosesson MW

更新日期：1992-01-28 00:00:00

abstract：:Olivopontocerebellar atrophy with retinal degeneration is a hereditary neurodegenerative disorder that belongs to the subtype II of the autosomal dominant cerebellar ataxias and is characterized by early-onset cerebellar and macular degeneration preceded by diagnostically useful tritan colorblindness. The gene mutated...

journal_title：Biochemistry

authors： Mushegian AR,Vishnivetskiy SA,Gurevich VV

更新日期：2000-06-13 00:00:00

abstract：:(R)-N(delta)-(N'-Sulfodiaminophosphinyl)-L-ornithine (PSorn) is the active component of a phytotoxin, called phaseolotoxin, produced by Pseudomonas savastanoi pv. phaseolicola. PSorn acts as a potent transition state (TS) inhibitor of ornithine transcarbamoylase (OTCase, E.C. 2.1.3.3) that binds to the OTCase from Esc...

journal_title：Biochemistry

authors： Templeton MD,Reinhardt LA,Collyer CA,Mitchell RE,Cleland WW

更新日期：2005-03-22 00:00:00

abstract：:Thermostable direct hemolysin (TDH) of Vibrio parahemolyticus is a membrane-damaging pore-forming toxin with potent cytolytic/cytotoxic activity. TDH exists as a tetramer consisting of protomers with a core β-sandwich domain, flanked by an 11-amino acid long N-terminal region (NTR). This NTR could not be modeled in th...

journal_title：Biochemistry

authors： Kundu N,Verma P,Kumar A,Dhar V,Dutta S,Chattopadhyay K

更新日期：2020-02-04 00:00:00

abstract：:Light-induced GTP-dependent scattering changes are studied in suspensions of retinal disc membranes to which one or both of the purified proteins involved in the phototransduction mechanism (G-protein and cGMP phosphodiesterase) are reassociated; a scattering change which depends on the presence of both G-protein (G) ...

journal_title：Biochemistry

authors： Bennett N,Clerc A

更新日期：1992-02-18 00:00:00

abstract：:Previous studies of Escherichia coli dihydrofolate reductase (ecDHFR) have demonstrated that residue G121, which is 19 A from the catalytic center, is involved in catalysis, and long distance dynamical motions were implied. Specifically, the ecDHFR mutant G121V has been extensively studied by various experimental and ...

journal_title：Biochemistry

authors： Wang L,Tharp S,Selzer T,Benkovic SJ,Kohen A

更新日期：2006-02-07 00:00:00

abstract：:The effects of Ca2+, Mg2+, and troponin I (TnI) inhibitory peptide (Ip) binding on the spectral properties of a Phe-154 to Trp mutant (F154W) of chicken recombinant troponin C (rTnC) have been examined. Residue 154 is positioned in the final flanking helix H of metal binding site IV. Since there are no other Tyr or Tr...

journal_title：Biochemistry

authors： Chandra M,McCubbin WD,Oikawa K,Kay CM,Smillie LB

更新日期：1994-03-15 00:00:00

abstract：:The level of albumin mRNA in the normal Buffalo rat liver and Morris hepatoma 7777 was compared by molecular hybridization with albumin complementary DNA (cDNA) and translational assays. Albumin mRNA was found to be 10% of the total rat liver poly(A)-containing RNA population but reduced approximately fourfold in the ...

journal_title：Biochemistry

authors： Tse TP,Morris HP,Taylor JM

更新日期：1978-07-25 00:00:00

abstract：:Aminoacylation of tRNA by aminoacyl-tRNA synthetases is the essential reaction that matches protein amino acids with the trinucleotide sequences specified in mRNA. Direct electrostatic interactions made by tRNA synthetases with discriminating functional groups on the tRNA bases have long been known to determine aminoa...

journal_title：Biochemistry

authors： Perona JJ,Hou YM

更新日期：2007-09-18 00:00:00

abstract：:The role of reversible phosphorylation in histamine-induced Ca2+ oscillations in HeLa cells has been investigated by using various activators and inhibitors of protein kinases and phosphatases. Electroporation was employed to introduce impermeable materials into single cells, which proved to be a useful and convenient...

journal_title：Biochemistry

authors： Zhu DM,Tekle E,Chock PB,Huang CY

更新日期：1996-06-04 00:00:00

abstract：:Oxidized cytochrome c oxidase exists in two reasonably well-defined conformations, a high-spin conformation with maximal absorption at 418 nm and a low-spin conformation with maximal absorption at 428 nm. The equilibrium between these two conformations has been studied as a function of pH, pressure, and temperature. p...

journal_title：Biochemistry

authors： Kornblatt JA,Hui Bon Hoa G

更新日期：1982-10-26 00:00:00

abstract：:The photoreceptor protein rhodopsin has been reconstituted with a single phospholipid species, dimyristoylphosphatidylcholine, at a range of different lipid/protein ratios, and the exchange rate at the lipid-protein interface has been determined from the electron spin resonance spectra of spin-labeled phosphatidylchol...

journal_title：Biochemistry

authors： Ryba NJ,Horváth LI,Watts A,Marsh D

更新日期：1987-06-02 00:00:00

abstract：:One- and two-dimensional NMR experiments were carried out on a decamer, d-(CGCTTTTCGC).d(GCGAAAAGCG), and on the same sequence with the addition of an unpaired thymidine, d(CGCTTTTCGC).d(GCGAATAAGCG), which will be referred to as the T-bulge decamer. Evidence from one-dimensional NOE experiments on the exchangeable pr...

journal_title：Biochemistry

authors： Morden KM,Gunn BM,Maskos K

更新日期：1990-09-18 00:00:00

abstract：:Previous studies of the low molecular mass family 11 xylanase from Bacillus circulans show that the ionization state of the nucleophile (Glu78, pK(a) 4.6) and the acid/base catalyst (Glu172, pK(a) 6.7) gives rise to its pH-dependent activity profile. Inspection of the crystal structure of BCX reveals that Glu78 and Gl...

journal_title：Biochemistry

authors： Joshi MD,Sidhu G,Nielsen JE,Brayer GD,Withers SG,McIntosh LP

更新日期：2001-08-28 00:00:00

abstract：:The light-induced global conformational change of photoactive yellow protein was directly observed by small-angle X-ray scattering (SAXS). The N-terminal 6, 15, or 23 amino acid residues were enzymatically truncated (T6, T15, or T23, respectively), and their near-UV intermediates were accumulated under continuous illu...

journal_title：Biochemistry

authors： Imamoto Y,Kamikubo H,Harigai M,Shimizu N,Kataoka M

更新日期：2002-11-19 00:00:00

abstract：:FLI1 (Friend leukemia integration 1) is a metazoan transcription factor that is upregulated in a number of cancers. In addition, rearrangements of the fli1 gene cause sarcomas, leukemias, and lymphomas. These rearrangements encode oncogenic transcription factors, in which the DNA binding domain (DBD or ETS domain) of ...

journal_title：Biochemistry

authors： Hou C,Tsodikov OV

更新日期：2015-12-22 00:00:00

abstract：:Here, we biochemically characterize Dictyostelium myosin II mutants that were previously phenotypically selected following random mutagenesis and shown to lie in the actin binding domain [Patterson, B., & Spudich, J. A. (1996) Genetics 143, 801-810]. We show that the conditional loss of myosin-dependent activity in vi...

journal_title：Biochemistry

authors： Giese KC,Spudich JA

更新日期：1997-07-15 00:00:00