Factors affecting gamma-chain multimer formation in cross-linked fibrin.

abstract：:We mutated residue 166, located in the putative Na(+) transport pathway between transmembrane segments 4 and 5 of human Na(+)/glucose cotransporter (hSGLT1), from alanine to cysteine (A166C). A166C was expressed in Xenopus laevis oocytes, and electrophysiological methods were used to assay function. The affinity for N...

journal_title：Biochemistry

authors： Meinild AK,Loo DD,Hirayama BA,Gallardo E,Wright EM

更新日期：2001-10-02 00:00:00

abstract：:Electron transfer within cytochrome cd1 from Thiosphaera pantotropha was investigated by the technique of pulse radiolysis. The reduction of the heme centers in this nitrite reductase occurred in two phases as judged from kinetic difference spectra. In the faster phase, radiolytically generated N-methylnicotinamide (N...

journal_title：Biochemistry

authors： Kobayashi K,Koppenhöfer A,Ferguson SJ,Tagawa S

更新日期：1997-11-04 00:00:00

abstract：:Metabolomics is a rapidly growing field of research used in the identification and quantification of the small molecule metabolites within an organism, thereby providing insights into cell metabolism and bioenergetics as well as processes important in clinical medicine, such as disposition of pharmaceutical compounds....

journal_title：Biochemistry

authors： Milne SB,Mathews TP,Myers DS,Ivanova PT,Brown HA

更新日期：2013-06-04 00:00:00

abstract：:Group II introns self-splice via a two-step mechanism: cleavage at the 5' splice site followed by exon ligation at the 3' splice site. The second step has been difficult to study in vitro because it is generally faster than the first. Herein we describe development and partial kinetic characterization of a novel assay...

journal_title：Biochemistry

authors： Bar-Shalom A,Moore MJ

更新日期：2000-08-22 00:00:00

abstract：:The partition of cis-parinaric acid (9,11,13,15-cis, trans, trans,cis-octadecatetraenoic acid, cis-PnA) and trans-parinaric acid (9,11,13,15-all-trans-octadecatetraenoic acid, trans-PnA) among aqueous, solid lipid, and fluid lipid phases has been measured by three spectroscopic parameters: absorption spectral shifts, ...

journal_title：Biochemistry

authors： Sklar LA,Miljanich GP,Dratz EA

更新日期：1979-05-01 00:00:00

abstract：:Glutathionylation of intracellular proteins is an established physiological regulator of protein function. In multiple models, including ischemia-reperfusion of the heart, increased oxidative stress results in the glutathionylation of sarcomeric actin. We hypothesized that actin glutathionylation may play a role in th...

journal_title：Biochemistry

authors： Pizarro GO,Ogut O

更新日期：2009-08-11 00:00:00

abstract：:Murine cDNA that encodes neuromodulin, a neurospecific calmodulin binding protein, was inserted into the plasmid pKK223-3 for expression in Escherichia coli. After being transformed into E. coli strain SG20252 (lon-), the expression vector directed the synthesis of a protein that was recognized by polyclonal antibodie...

journal_title：Biochemistry

authors： Au DC,Apel ED,Chapman ER,Estep RP,Nicolson TA,Storm DR

更新日期：1989-10-03 00:00:00

abstract：:Many biological processes are now known to be regulated by Ca2+ via calmodulin (CM). Although a general mechanistic model by which Ca2+ and calmodulin modulate many of these activities has been proposed, an accurate quantitative model is not available. A detailed analysis of skeletal muscle myosin light chain kinase a...

journal_title：Biochemistry

authors： Blumenthal DK,Stull JT

更新日期：1980-11-25 00:00:00

abstract：:Molecular imaging is an important new direction in medical diagnosis; however, its success is dependent upon molecular probes that demonstrate selective tissue targeting. We report the design and chemical synthesis of a derivative of human amyloid-beta (Abeta) peptide that is capable of selectively targeting individua...

journal_title：Biochemistry

authors： Poduslo JF,Curran GL,Peterson JA,McCormick DJ,Fauq AH,Khan MA,Wengenack TM

更新日期：2004-05-25 00:00:00

abstract：:Purine repressor (PurR) binding to specific DNA is enhanced by complexing with purines, whereas lactose repressor (LacI) binding is diminished by interaction with inducer sugars despite 30% identity in their protein sequences and highly homologous tertiary structures. Nonetheless, in switching from low- to high-affini...

journal_title：Biochemistry

authors： Moraitis MI,Xu H,Matthews KS

更新日期：2001-07-10 00:00:00

abstract：:Casein kinase II is a widely distributed protein serine/threonine kinase. The holoenzyme appears to be a tetramer, containing two alpha or alpha' subunits (or one of each) and two beta subunits. Complementary DNA clones encoding the subunits of casein kinase II were isolated from a human T-cell lambda gt10 library usi...

journal_title：Biochemistry

authors： Lozeman FJ,Litchfield DW,Piening C,Takio K,Walsh KA,Krebs EG

更新日期：1990-09-11 00:00:00

abstract：:Venomous organisms have evolved a variety of structurally diverse peptide neurotoxins that target ion channels. Despite the lack of any obvious structural homology, unrelated toxins that interact with voltage-activated K(+) channels share a dyad motif composed of a lysine and a hydrophobic amino acid residue, usually ...

journal_title：Biochemistry

authors： Al-Sabi A,Lennartz D,Ferber M,Gulyas J,Rivier JE,Olivera BM,Carlomagno T,Terlau H

更新日期：2004-07-13 00:00:00

abstract：:The reduction of substrates catalyzed by nitrogenase normally requires nucleotide-dependent Fe protein delivery of electrons to the MoFe protein, which contains the active site FeMo cofactor. Here, it is reported that independent substitution of three amino acids (β-98(Tyr→His), α-64(Tyr→His), and β-99(Phe→His)) locat...

journal_title：Biochemistry

authors： Danyal K,Rasmussen AJ,Keable SM,Inglet BS,Shaw S,Zadvornyy OA,Duval S,Dean DR,Raugei S,Peters JW,Seefeldt LC

更新日期：2015-04-21 00:00:00

abstract：:Although insulin and insulin-like growth factor-1 (IGF-1) belong to one family, insulin folds into one thermodynamically stable structure, while IGF-1-folds into two thermodynamically stable structures (native and swap forms). We have demonstrated previously that the bifurcating folding behavior of IGF-1 is mainly con...

journal_title：Biochemistry

authors： Chen Y,You Y,Jin R,Guo ZY,Feng YM

更新日期：2004-07-20 00:00:00

abstract：:A 16-base pair oligo(purine)-oligo(pyrimidine) sequence present in the coding region of two HIV 1 proviral genes (pol and nef) was chosen as a target for triplex-forming oligonucleotides in in vitro transcription assays. Inhibition of transcription elongation was observed with triplex-forming oligonucleotide-acridine ...

journal_title：Biochemistry

authors： Giovannangeli C,Perrouault L,Escudé C,Nguyen T,Hélène C

更新日期：1996-08-13 00:00:00

abstract：:Sarcoplasmic phosphorylase phosphatase extracted from ground skeletal muscle was recovered in a high molecular weight from (Mr = 250000). This enzyme has been purified from extracts by anion-exchange and gel chromatography to yield a preparation with three major protein components of Mr 83000, 72000, and 32000 by sodi...

journal_title：Biochemistry

authors： Brautigan DL,Picton C,Fischer EH

更新日期：1980-12-09 00:00:00

abstract：:The circular dichroism (CD) of Pfl filamentous virus has been examined over the temperature range 0-40 degrees C, in the absence and presence of Hg(II), Ag(I), and Cu(II). Thermal difference CD spectra were obtained by subtraction of spectra recorded above and below a thermally induced structure transition near 12 deg...

journal_title：Biochemistry

authors： Casadevall A,Day LA

更新日期：1988-05-17 00:00:00

abstract：:Recently, we synthesized the first individual β,γ-CHX-dGTP diastereomers [(R)- or (S)-CHX, where X is F or Cl] and determined their structures in ternary complexes with DNA polymerase β (pol β). We now report stereospecificity by pol β on the mixed β,γ-CHX diastereomer pairs using nuclear magnetic resonance and on the...

journal_title：Biochemistry

authors： Oertell K,Wu Y,Zakharova VM,Kashemirov BA,Shock DD,Beard WA,Wilson SH,McKenna CE,Goodman MF

更新日期：2012-10-30 00:00:00

abstract：:The chemical and enzymatic syntheses of bacteriophage lambda pseudo operator DNA are described. The 17 base-paired duplex contains the DNA which has been proposed as the binding site for cI repressor protein. The synthetic duplex is twofold symmetric and represents the best possible nucleotide summation of the six pro...

journal_title：Biochemistry

authors： Kawashima E,Gadek T,Caruthers MH

更新日期：1977-09-20 00:00:00

abstract：:The paramagnetic enhancements in the NMR relaxation rates for the fluorine in fluorophthalates have been used to determine the position of the phthalate with respect to the mononuclear metal ion in native and metal-substituted derivatives of phthalate dioxygenase (PDO). These studies show directly that the substrate i...

journal_title：Biochemistry

authors： Tierney DL,Gassner GT,Luchinat C,Bertini I,Ballou DP,Penner-Hahn JE

更新日期：1999-08-24 00:00:00

abstract：:For the elucidation of the mechanism of steroid hormone receptor activation, the hydrodynamic properties of the unactivated and activated forms of the nonproteolyzed glucocorticoid receptor from the mouse AtT-20 pituitary tumor cell line were determined. The unactivated, molybdate-stabilized receptor has the following...

journal_title：Biochemistry

authors： Vedeckis WV

更新日期：1983-04-12 00:00:00

abstract：:The structures of oligosaccharides of normal and pathological immunoglobulin G (IgG) are reported. Asparagine-linked neutral oligosaccharides were released by N-oligosaccharide glycopeptidase (almond) digestion. The reducing ends of the oligosaccharide chains thus obtained were aminated with a fluorescent reagent, 2-a...

journal_title：Biochemistry

authors： Takahashi N,Ishii I,Ishihara H,Mori M,Tejima S,Jefferis R,Endo S,Arata Y

更新日期：1987-02-24 00:00:00

abstract：:Recombinant human 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthetase, isoform 1 (brain), was purified to near-homogeneity from an Escherichia coli expression system and kinetically characterized. The native enzyme, a dimer with each 71 kDa subunit containing an adenosine triphosphate (ATP) sulfurylase and an aden...

journal_title：Biochemistry

authors： Lansdon EB,Fisher AJ,Segel IH

更新日期：2004-04-13 00:00:00

abstract：:A high degree of selectivity toward the target site of the pest organism is a desirable attribute for new safer agrochemicals. To assist in the design of novel herbicides, we determined the crystal structures of the herbicidal target enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD; EC 1.13.11.27) from the plant Arabi...

journal_title：Biochemistry

authors： Yang C,Pflugrath JW,Camper DL,Foster ML,Pernich DJ,Walsh TA

更新日期：2004-08-17 00:00:00

abstract：:Oocytes released en masse from pig ovaries were isolated in large quantities by using sieving techniques. The isolated oocytes were gently homogenized, and the largely intact zona pellucida "ghosts" were purified by using sieving techniques. Sufficient amounts of zonae were recovered to permit, for the first time, ade...

journal_title：Biochemistry

authors： Dunbar BS,Wardrip NJ,Hedrick JL

更新日期：1980-01-22 00:00:00

abstract：:SHP (short heterodimer partner) is an orphan nuclear receptor, first described for its interaction with nuclear receptors. This study explores a new way of inhibiting the androgen-signaling pathway. We demonstrated that SHP inhibited up to 97% of AR-induced activity. Characterization of AR/SHP interaction provided evi...

journal_title：Biochemistry

authors： Gobinet J,Auzou G,Nicolas JC,Sultan C,Jalaguier S

更新日期：2001-12-18 00:00:00

abstract：:The presence and subcellular localization of UDP-Gal:glucosylceramide beta 1----4galactosyltransferase (GalT-2) was investigated in rat liver. For this purpose, purified Golgi apparatus, endoplasmic reticulum, and plasma membrane fractions were prepared from the liver and used as the enzyme source for detecting GalT-2...

journal_title：Biochemistry

authors： Trinchera M,Fiorilli A,Ghidoni R

更新日期：1991-03-12 00:00:00

abstract：:The anomeric proton (H-1) chemical shifts of D-mannopyranosides in aqueous solution are affected both by the aglycon and by substitution of the ring [Lee, Y. C., & Ballou, C. E. (1965) Biochemistry 4, 257]. We have examined the 1H NMR spectra for a variety of linear and branched mannooligosaccharides and have assigned...

journal_title：Biochemistry

authors： Cohen RE,Ballou CE

更新日期：1980-09-02 00:00:00

abstract：:The family 4 uracil-DNA glycosylase from the hyperthermophilic organism Archaeoglobus fulgidus (AFUDG) is responsible for the removal of uracil in DNA as the first step in the base excision repair (BER) pathway. AFUDG contains a large solvent-exposed peptide region containing an α helix and loop anchored on each end v...

journal_title：Biochemistry

authors： Engstrom LM,Partington OA,David SS

更新日期：2012-06-26 00:00:00

abstract：:The mechanism of binding of reduced 1-(2-carboxyphenylamino)-1-deoxyribulose 5-phosphate (rCdRP) to two different binding sites on the bifunctional enzyme is determined by kinetic studies, using temperature-jump and stopped-flow equipment with fluorescence detection. Two rapid binding processes and a comparatively slo...

journal_title：Biochemistry

authors： Cohn W,Kirschner K,Paul C

更新日期：1979-12-25 00:00:00