An all-D amino acid peptide model of alpha1(IV)531-543 from type IV collagen binds the alpha3beta1 integrin and mediates tumor cell adhesion, spreading, and motility.

abstract：:Oxindolealanine-62 lysozyme, formed by reaction with N-bromosuccinimide and purified by using affinity chromatography, was examined in its binding of homologous oligosaccharides of N-acetylglucosamine and in its catalysis of hydrolysis and of transglycosylation of the hexasaccharide of N-acetylglucosamine. Equilibrium...

journal_title：Biochemistry

authors： Shrake A,Rupley JA

更新日期：1980-08-19 00:00:00

abstract：:While calcium binding to troponin C (TnC) triggers the contraction of both skeletal and cardiac muscle, there is clear evidence that different mechanisms may be involved. For example, activation of heart myofilaments occurs with binding to a single regulatory site on TnC, whereas activation of fast skeletal myofilamen...

journal_title：Biochemistry

authors： Spyracopoulos L,Li MX,Sia SK,Gagné SM,Chandra M,Solaro RJ,Sykes BD

更新日期：1997-10-07 00:00:00

abstract：:Acholeplasma laidlawii B cells, highly enriched in saturated C14 and C15 fatty acids, have been grown at several temperatures. Conformational order in the acyl chains of live cell membranes has been monitored with Fourier-transform infrared (FT-IR) spectroscopy. Coupled CH2 wagging progressions, characteristic of the ...

journal_title：Biochemistry

authors： Moore DJ,Mendelsohn R

更新日期：1994-04-05 00:00:00

abstract：:The signaling apparatus that controls bacterial chemotaxis is composed of a core complex containing chemoreceptors, the histidine autokinase CheA, and the coupling protein CheW. Site-specific spin labeling and pulsed dipolar ESR spectroscopy (PDS) have been applied to investigate the structure of a soluble ternary com...

journal_title：Biochemistry

authors： Bhatnagar J,Borbat PP,Pollard AM,Bilwes AM,Freed JH,Crane BR

更新日期：2010-05-11 00:00:00

abstract：:The PB1 domain of NBR1 folds via a single pathway mechanism involving two sequential energy barriers separated by a high-energy intermediate. The structural ensemble representing each of the two transition states (TS1 and TS2) has been calculated using experimental Φ values and biased molecular dynamics simulations. B...

journal_title：Biochemistry

authors： Chen P,Evans CL,Hirst JD,Searle MS

更新日期：2011-01-11 00:00:00

abstract：:Phosphorescence from the lone tryptophan residue has been studied to monitor the interaction of myelin basic protein with phosphatidylserine vesicles. Spectral shifts in the phosphorescence of the protein in a glycerol-buffer (70:30 w/w) solvent at low temperature are consistent with fluorescence data obtained under a...

journal_title：Biochemistry

authors： Vadas EB,Melançon P,Braun PE,Galley WC

更新日期：1981-05-26 00:00:00

abstract：:The temperature dependence of the 31P NMR spectra of yeast phenylalanine tRNA, E. coli tyrosine, glutamate (2), and formylmethionine tRNA is presented. The major difference between the 31P NMR spectra of the different acceptor tRNAs is in the main cluster region between -0.5 and -1.3 ppm. This confirms an earlier assi...

journal_title：Biochemistry

authors： Gorenstein DG,Goldfield EM

更新日期：1982-11-09 00:00:00

abstract：:DNA ring closure experiments on short restriction fragments ( approximately 160 bp) bound by the TATA box binding protein (TBP) have demonstrated the formation of negative topoisomers, consistent with crystallographically observed TBP-induced DNA untwisting but in contrast to most previous results on topological effec...

journal_title：Biochemistry

authors： Kahn JD

更新日期：2000-04-04 00:00:00

abstract：:Myocardial contraction is initiated when Ca2+ binds to site II of cardiac troponin C. This 12-residue EF-hand loop (NH2-DEDGSGTVDFDE-COOH) contains six residues (bold) that coordinate Ca2+ binding and six residues that do not appear to influence Ca2+ binding directly. We have introduced six single-cysteine substitutio...

journal_title：Biochemistry

authors： Reece KL,Moss RL

更新日期：2008-05-06 00:00:00

abstract：:The three subunits I, II, and III of bovine procarboxypeptidase A separated by reversible dimethylmaleylation can reassociate to form the reconstituted complexes I + II, I + III, and I + II + III. Since the association II + III is not possible, subunit I appears to play a central role in the formation of the complex. ...

journal_title：Biochemistry

authors： Puigserver A,Desnuelle P

更新日期：1977-05-31 00:00:00

abstract：:The protein tyrosine kinase p72syk readily phosphorylates hematopoietic linkage cell-specific protein p50/HS1 with high stoichiometry (up to 4 mol of Pi/mol of protein) and favorable kinetic constants (Km 77 nM, kcat 0.37 s-1), at sites that display the motif that is specifically recognized by the HS2 domains of Src t...

journal_title：Biochemistry

authors： Ruzzene M,Brunati AM,Marin O,Donella-Deana A,Pinna LA

更新日期：1996-04-23 00:00:00

abstract：:Biotin synthase, the enzyme which catalyzes the last step of the biosynthesis of biotin, contains only (2Fe-2S)(2+) clusters when isolated under aerobic conditions. Previous results showed that reduction by dithionite or photoreduced deazaflavin converts the (2Fe-2S)(2+) to (4Fe-4S)(2+,+). However, until now, no detai...

journal_title：Biochemistry

authors： Tse Sum Bui B,Benda R,Schünemann V,Florentin D,Trautwein AX,Marquet A

更新日期：2003-07-29 00:00:00

abstract：:An overexpression system for spinach apocytochrome b(6) as a fusion protein to a maltose-binding protein in Escherichia coli was established using the expression vector pMalp2. The fusion of the cytochrome b(6) to the periplasmic maltose-binding protein directs the cytochrome on the Sec-dependent pathway. The cytochro...

journal_title：Biochemistry

authors： Króliczewski J,Hombek-Urban K,Szczepaniak A

更新日期：2005-05-24 00:00:00

abstract：:The mechanism of herpesviral protease activation upon dimerization was studied using two independent spectroscopic assays augmented by directed mutagenesis. Spectroscopic changes, attributable to dimer interface conformational plasticity, were observed upon dimerization of Kaposi's sarcoma-associated herpesvirus prote...

journal_title：Biochemistry

authors： Pray TR,Reiling KK,Demirjian BG,Craik CS

更新日期：2002-02-05 00:00:00

abstract：:A likely entry/exit path for nitrogenase substrates, products, and/or protons involves residues α277(Arg), α192(Ser), and α356(Gly), all of which are highly conserved among MoFe proteins from different organisms. The α192(Ser) and α277(Arg) residues form part of a hydrogen-bonded network that also involves α195(His), ...

journal_title：Biochemistry

authors： Fisher K,Hare ND,Newton WE

更新日期：2014-10-07 00:00:00

abstract：:Benzoylformate decarboxylase (BFDC) and pyruvate decarboxylase (PDC) are both thiamin diphosphate-dependent enzymes. The two share a common three-dimensional structure and catalyze a similar chemical reaction, i.e., decarboxylation of 2-keto acids. However, they vary significantly in their substrate utilization patter...

journal_title：Biochemistry

authors： Yep A,McLeish MJ

更新日期：2009-09-08 00:00:00

abstract：:A two-step pathway consisting of an acyl-acyl carrier protein (ACP) reductase (AAR) and an aldehyde-deformylating oxygenase (ADO) allows various cyanobacteria to convert long-chain fatty acids into hydrocarbons. AAR catalyzes the two-electron, NADPH-dependent reduction of a fatty acid attached to ACP via a thioester l...

journal_title：Biochemistry

authors： Warui DM,Pandelia ME,Rajakovich LJ,Krebs C,Bollinger JM Jr,Booker SJ

更新日期：2015-02-03 00:00:00

abstract：:The Ca2+-dependent regulation of smooth muscle actomyosin involves a myosin light chain kinase (ATP: myosin light chain phosphotransferase). It has been shown (Dabrowska, R., Aromatorio, D., Sherry, J.M.F., and Hartshorne, D.J. 1977, Biochem. Biophys. Res. Commun. 78, 1263) that the kinase is composed of two proteins ...

journal_title：Biochemistry

authors： Dabrowska R,Sherry JM,Aromatorio DK,Hartshorne DJ

更新日期：1978-01-24 00:00:00

abstract：:Membranes derived from free floating granulosa cells in porcine ovarian follicular fluid were used as a starting material for structural characterization of both LH/hCG and FSH receptors. The receptors were highly hormone-specific and showed single classes of high-affinity binding sites (Kd = 19-74 pM). Their molecula...

journal_title：Biochemistry

authors： Yarney TA,Sairam MR,Bhargavi GN,Mohapatra SK

更新日期：1990-04-17 00:00:00

abstract：:Loop 181-197 of human thymidylate synthase (hTS) populates two conformational states. In the first state, Cys195, a residue crucial for catalytic activity, is in the active site (active conformer); in the other conformation, it is about 10 A away, outside the active site (inactive conformer). We have designed and expr...

journal_title：Biochemistry

authors： Gibson LM,Lovelace LL,Lebioda L

更新日期：2008-04-22 00:00:00

abstract：:Beetle luciferases produce different bioluminescence colors from green to red using the same d-luciferin substrate. Despite many studies of the mechanisms and structural determinants of bioluminescence colors with firefly luciferases, the identity of the emitters and the specific active site interactions responsible f...

journal_title：Biochemistry

authors： Viviani VR,Neves DR,Amaral DT,Prado RA,Matsuhashi T,Hirano T

更新日期：2014-08-19 00:00:00

abstract：:Structures of glycopeptides obtained by exhaustive Pronase digestion of high molecular weight (1.7 X 10(5)) salmon egg polysialoglycoprotein have been elucidated. Six principal glycopeptides isolated by gel chromatography and DEAE-Sephadex A-25 chromatography in the absence or presence of borate ion were analyzed for ...

journal_title：Biochemistry

authors： Shimamura M,Inoue Y,Inoue S

更新日期：1985-09-24 00:00:00

abstract：:Corticosteroid binding globulin (CBG), a serum glycoprotein which binds glucocorticoids and progestins with high affinity, is widely distributed throughout the animal world. Although its charge and size characteristics have largely been conserved across species, we found the behavior of CBG in squirrel monkey (Saimiri...

journal_title：Biochemistry

authors： Kuhn RW,VestWeber C,Siiteri PK

更新日期：1988-04-05 00:00:00

abstract：:The integral membrane protein diacylglycerol kinase (DGK) from Escherichia coli has been reversibly unfolded in a protein/detergent/mixed micelle system by varying the molar ratio of n-decyl beta-D-maltoside (DM) and sodium dodecyl sulfate (SDS). Unfolding was monitored by circular dichroism (CD) and ultraviolet (UV) ...

journal_title：Biochemistry

authors： Lau FW,Bowie JU

更新日期：1997-05-13 00:00:00

abstract：:As discovered by Grazi & Trombetta [Grazi, E., & Trombetta, G. (1978) Biochem. J. 175, 361], fructose-1,6-bisphosphate aldolase of rabbit muscle causes the slow formation of inorganic phosphate (Pi) and methylglyoxal when incubated with dihydroxyacetone phosphate (DHAP). In addition, these authors found an acid-labile...

journal_title：Biochemistry

authors： Iyengar R,Rose IA

更新日期：1981-03-03 00:00:00

abstract：:Peptides and peptide-like molecules as a class have very poor permeability through biological membranes, which severely compromises their potential effectiveness as therapeutic agents. In order to gain insight into the problem of delivering peptide and protein drugs and to establish a model in which the effects of sys...

journal_title：Biochemistry

authors： Gray RA,Vander Velde DG,Burke CJ,Manning MC,Middaugh CR,Borchardt RT

更新日期：1994-02-15 00:00:00

abstract：:Calcium-saturated calmodulin (CaM) binds and influences the activity of a varied collection of target proteins in most cells. This promiscuity underlies the role of CaM as a shared participant in calcium-dependent signal transduction pathways but imposes a handicap on popular CaM-based calcium biosensors, which displa...

journal_title：Biochemistry

authors： Green DF,Dennis AT,Fam PS,Tidor B,Jasanoff A

更新日期：2006-10-17 00:00:00

abstract：:The iminodihydroquinoline WIN 17317-3 was previously shown to inhibit selectively the voltage-gated potassium channels, K(v)1.3 and K(v)1.4 [Hill, R. J., et al. (1995) Mol. Pharmacol. 48, 98-104; Nguyen, A., et al. (1996) Mol. Pharmacol. 50, 1672-1679]. Since these channels are found in brain, radiolabeled WIN 17317-3...

journal_title：Biochemistry

authors： Wanner SG,Glossmann H,Knaus HG,Baker R,Parsons W,Rupprecht KM,Brochu R,Cohen CJ,Schmalhofer W,Smith M,Warren V,Garcia ML,Kaczorowski GJ

更新日期：1999-08-24 00:00:00

abstract：:The fluorescent pH indicator 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to investigate changes in the intracellular pH (pHi) of individual Sf9 cells in response to changes in the composition of the external medium. Under standard conditions, the resting pHi was 0.2-0.3 unit lower than the extracel...

journal_title：Biochemistry

authors： Vachon V,Paradis MJ,Marsolais M,Schwartz JL,Laprade R

更新日期：1995-11-21 00:00:00

abstract：:Tandem DNA lesions containing two contiguously damaged nucleotides are commonly formed by ionizing radiation. Their effects on replication in mammalian cells are largely unknown. Replication of isolated 2-deoxyribonolactone (L), thymine glycol (Tg), and tandem lesion 5'-LTg was examined in human cells. Although nearly...

journal_title：Biochemistry

authors： Naldiga S,Huang H,Greenberg MM,Basu AK

更新日期：2020-02-04 00:00:00