当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > High-resolution phosphorus nuclear magnetic resonance spectroscopy of transfer ribonucleic acids: multiple conformations in the anticodon loop.

High-resolution phosphorus nuclear magnetic resonance spectroscopy of transfer ribonucleic acids: multiple conformations in the anticodon loop.

Abstract：

:The temperature dependence of the 31P NMR spectra of yeast phenylalanine tRNA, E. coli tyrosine, glutamate (2), and formylmethionine tRNA is presented. The major difference between the 31P NMR spectra of the different acceptor tRNAs is in the main cluster region between -0.5 and -1.3 ppm. This confirms an earlier assignment of the main cluster region to the undistorted phosphate diesters in the hairpin loops and helical stems. In addition the 31P NMR spectra for all tRNAs reveal approximately 16 nonhelical diester signals spread over approximately 7 ppm besides the downfield terminal 3'-phosphate monoester. In the presence of 10 mM Mg2+ most scattered and main cluster signals do not shift between 22 and 66 degrees C, thus supporting our earlier hypothesis that 31P chemical shifts are sensitive to phosphate ester torsional and bond angles. At greater than 70 degrees C, all of the signals merge into a single random-coil conformation signal. A number of the scattered peaks are shifted (0.2-1.7 ppm) and broadened between 22 and 66 degrees C in the presence of Mg2+ and spermine as a result of a conformational transition in the anticodon loop. The 31P NMR spectrum of the dimer formed between yeast tRNAPhe and E. coli tRNA 2Glu is reported. This dimer simulates codon-anticodon interaction since the anticodon triplets of the two tRNAs are complementary. Evidence is presented that the anticodon-anticodon interaction alters the anticodon conformation and partially disrupts the tertiary structure of the tRNA.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Gorenstein DG,Goldfield EM

doi

10.1021/bi00266a018

subject

Has Abstract

pub_date

1982-11-09 00:00:00

pages

5839-49

issue

23

eissn

0006-2960

issn

1520-4995

journal_volume

21

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Electron/proton coupling in bacterial nitric oxide reductase during reduction of oxygen.

    abstract：:The respiratory nitric oxide reductase (NOR) from Paracoccus denitrificans catalyzes the two-electron reduction of NO to N(2)O (2NO + 2H(+) + 2e(-) --> N(2)O + H(2)O), which is an obligatory step in the sequential reduction of nitrate to dinitrogen known as denitrification. NOR has four redox-active cofactors, namely,...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050524h

    authors： Flock U,Watmough NJ,Adelroth P

    更新日期：2005-08-09 00:00:00

  • Multiple enzyme activities of Escherichia coli MutT protein for sanitization of DNA and RNA precursor pools.

    abstract：:8-OxoGua (8-oxo-7,8-dihydroguanine) is produced in nucleic acids as well as in nucleotide pools of cells, by reactive oxygen species normally formed during cellular metabolic processes. MutT protein of Escherichia coli specifically degrades 8-oxoGua-containing deoxyribo- and ribonucleoside triphosphates to correspondi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi047550k

    authors： Ito R,Hayakawa H,Sekiguchi M,Ishibashi T

    更新日期：2005-05-03 00:00:00

  • Differential scanning calorimetric study of the thermal unfolding of mutant forms of phage T4 lysozyme.

    abstract：:In two recent papers, we reported the effects of several point mutations on the thermodynamics of the thermal unfolding of the lysozyme of phage T4 as determined by differential scanning calorimetry. The mutants studied were R96H [Kitamura, S., & Sturtevant, J.M. (1989) Biochemistry 28, 3788-3792] and T157 replaced by...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00121a009

    authors： Hu CQ,Kitamura S,Tanaka A,Sturtevant JM

    更新日期：1992-02-18 00:00:00

  • Fluorescence spectroscopic studies of pressure effects on Na+,K(+)-ATPase reconstituted into phospholipid bilayers and model raft mixtures.

    abstract：:To contribute to the understanding of membrane protein function upon application of pressure as relevant for understanding, for example, the physiology of deep sea organisms or for baroenzymological biotechnical processes, we investigated the influence of hydrostatic pressure on the activity of Na+,K+-ATPase enriched ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi062235e

    authors： Powalska E,Janosch S,Kinne-Saffran E,Kinne RK,Fontes CF,Mignaco JA,Winter R

    更新日期：2007-02-13 00:00:00

  • DnaB helicase affects the initiation specificity of Escherichia coli primase on single-stranded DNA templates.

    abstract：:The effect of DnaB helicase on the initiation specificity of primase was studied biochemically using a series of single-stranded DNA templates in which each nucleotide of the trinucleotide d(CTG) initiation sequence was systematically varied. DnaB helicase accelerated the rate of primer syntheisis, prevented "overlong...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991555d

    authors： Bhattacharyya S,Griep MA

    更新日期：2000-02-01 00:00:00

  • Crystallographic studies of isosteric NAD analogues bound to alcohol dehydrogenase: specificity and substrate binding in two ternary complexes.

    abstract：:CNAD (5-beta-D-ribofuranosylnicotinamide adenine dinucleotide) is an isosteric C-glycosidic analogue of NAD(H) containing a neutral pyridine ring. CPAD (5-beta-D-ribofuranosylpicolinamide adenine dinucleotide) is a closely related pyridine-containing analogue with the pyridine nitrogen on the opposite side of the ring...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00205a009

    authors： Li H,Hallows WH,Punzi JS,Pankiewicz KW,Watanabe KA,Goldstein BM

    更新日期：1994-10-04 00:00:00

  • Complete assignment of disulfide bonds in bovine dopamine beta-hydroxylase.

    abstract：:Peptide mapping, chemical sequencing, microbore HPLC/electrospray ionization mass spectrometry (LC/ESI/MS), and matrix-assisted laser desorption mass spectrometry (MALDI/MS) were used to identify the sites of intra- and intermolecular disulfide linkages in bovine dopamine beta-hydroxylase. The enzyme contains 14 cyste...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00204a019

    authors： Robertson JG,Adams GW,Medzihradszky KF,Burlingame AL,Villafranca JJ

    更新日期：1994-09-27 00:00:00

  • Role of binding energy with coenzyme A in catalysis by 3-oxoacid coenzyme A transferase.

    abstract：:Succinyl-CoA:3-oxoacid coenzyme A transferase (EC 2.8.3.5), which catalyzes the reversible conversion of succinyl-CoA and acetoacetate into acetoacetyl-CoA and succinate through a covalent enzyme thiol ester intermediate, E-CoA, utilizes binding energy from noncovalent interactions with CoA to bring about an increase ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00037a005

    authors： Whitty A,Fierke CA,Jencks WP

    更新日期：1995-09-19 00:00:00

  • Recombinant DHX33 Protein Possesses Dual DNA/RNA Helicase Activity.

    abstract：:RNA helicase DHX33 has been shown to participate in a variety of cellular activities, including ribosome biogenesis, protein translation, and gene transcription. We and others further discovered that DHX33 is strongly expressed in several types of human cancers and plays important roles in promoting cancer cell prolif...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00166

    authors： Wang X,Ge W,Zhang Y

    更新日期：2019-01-29 00:00:00

  • Kinetic mechanism of DNA polymerase I (Klenow fragment): identification of a second conformational change and evaluation of the internal equilibrium constant.

    abstract：:In a previously determined minimal kinetic scheme for DNA polymerization catalyzed by the Klenow fragment (KF) of Escherichia coli DNA polymerase I, a nonchemical step that interconverted the KF'.DNAn+1.PPi and KF.DNAn+1PPi complexes was not observed in correct incorporation [Kuchta, R. D., Mizrahi, V., Benkovic, P.A....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00234a002

    authors： Dahlberg ME,Benkovic SJ

    更新日期：1991-05-21 00:00:00

  • Photochemical cross-linking of psoralen-derivatized oligonucleoside methylphosphonates to rabbit globin messenger RNA.

    abstract：:Antisense oligodeoxyribonucleoside methylphosphonates targeted against various regions of mRNA or precursor mRNA are selective inhibitors of mRNA expression both in cell-free systems and in cells in culture. The efficiency with which methylphosphonate oligomers interact with mRNA, and thus inhibit translation, can be ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00426a008

    authors： Kean JM,Murakami A,Blake KR,Cushman CD,Miller PS

    更新日期：1988-12-27 00:00:00

  • Alamethicin incorporation in lipid bilayers: a thermodynamic study.

    abstract：:Interaction of the peptide antibiotic alamethicin with phospholipid vesicles has been monitored by changes in its circular dichroic and fluorescent properties. The data are consistent with an incorporation of the peptide in the lipid bilayer. Aggregation of alamethicin in the membrane phase is evident from a character...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00384a015

    authors： Rizzo V,Stankowski S,Schwarz G

    更新日期：1987-05-19 00:00:00

  • Human aldose reductase: subtle effects revealed by rapid kinetic studies of the C298A mutant enzyme.

    abstract：:Transient kinetic data for D-xylose reduction with NADPH and NADPD and for xylitol oxidation with NADP+ catalyzed by recombinant C298A mutant human aldose reductase at pH 8 have been used to obtain estimates for each of the rate constants in the complete reaction mechanism as outlined for the wild-type enzyme in the p...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00044a013

    authors： Grimshaw CE,Bohren KM,Lai CJ,Gabbay KH

    更新日期：1995-11-07 00:00:00

  • Beta-tubulin isotypes purified from bovine brain have different relative stabilities.

    abstract：:The highly conserved nature and tissue specificity of the seven vertebrate beta-tubulin isotypes provide circumstantial evidence that functional differences among isotypes may exist in vivo. Compelling evidence from studies of bovine brain beta-isotypes indicated significant conformational and functional differences i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972763d

    authors： Schwarz PM,Liggins JR,Ludueña RF

    更新日期：1998-03-31 00:00:00

  • Structures of Escherichia coli histidinol-phosphate aminotransferase and its complexes with histidinol-phosphate and N-(5'-phosphopyridoxyl)-L-glutamate: double substrate recognition of the enzyme.

    abstract：:Histidinol-phosphate aminotransferase (HspAT) is a key enzyme on the histidine biosynthetic pathway. HspAT catalyzes the transfer of the amino group of L-histidinol phosphate (Hsp) to 2-oxoglutarate to form imidazole acetol phosphate (IAP) and glutamate. Thus, HspAT recognizes two kinds of substrates, Hsp and glutamat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002769u

    authors： Haruyama K,Nakai T,Miyahara I,Hirotsu K,Mizuguchi H,Hayashi H,Kagamiyama H

    更新日期：2001-04-17 00:00:00

  • Comparison of the crystal and solution structures of calmodulin and troponin C.

    abstract：:X-ray solution scattering data from skeletal muscle troponin C and from calmodulin have been measured. Modeling studies based on the crystal structure coordinates for these proteins show discrepancies between the solution data and the crystal structure that indicate that if the size and shape of the globular domains a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00403a011

    authors： Heidorn DB,Trewhella J

    更新日期：1988-02-09 00:00:00

  • Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 2. Unusual photophysical properties.

    abstract：:In the preceding accompanying paper [Shu, X., et al. (2007) Biochemistry 46, 12005-12013], the 1.5 A resolution crystal structure of green fluorescent protein (GFP) variant S65T/H148D is presented, and the possible consequences of an unusual short hydrogen bond (

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700904a

    authors： Shi X,Abbyad P,Shu X,Kallio K,Kanchanawong P,Childs W,Remington SJ,Boxer SG

    更新日期：2007-10-30 00:00:00

  • Dissociation of apolipoprotein E oligomers to monomer is required for high-affinity binding to phospholipid vesicles.

    abstract：:The apolipoprotein apoE plays a key role in cholesterol and lipid metabolism. There are three isoforms of this protein, one of which, apoE4, is the major risk factor for Alzheimer's disease. At micromolar concentrations all lipid-free apoE isoforms exist primarily as monomers, dimers, and tetramers. However, the molec...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1020106

    authors： Garai K,Baban B,Frieden C

    更新日期：2011-04-05 00:00:00

  • Restriction of the lateral motion of band 3 in the erythrocyte membrane by the cytoskeletal network: dependence on spectrin association state.

    abstract：:The effects of incubation of erythrocyte ghosts under various conditions (ionic strength or addition of ankyrin, diamines, or ATP) on the lateral motion of band 3 in the membranes were studied by using the fluorescence photobleaching recovery technique. Incubation of ghosts with exogenous ankyrin increased the immobil...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00368a045

    authors： Tsuji A,Ohnishi S

    更新日期：1986-10-07 00:00:00

  • Two promoters in the bovine adrenodoxin gene and the role of associated, unique cAMP-responsive sequences.

    abstract：:The bovine adrenodoxin gene gives rise to two species of mRNA differing only at their 5'-ends. The synthesis of these two types of mRNA in bovine adrenal cortical cells is regulated transcriptionally in part by ACTH via the action of cAMP. Examination of the 5'-end of the adrenodoxin gene revealed that each mRNA conta...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00123a027

    authors： Chen JY,Waterman MR

    更新日期：1992-03-03 00:00:00

  • Mycobacterium tuberculosis Rv0899 adopts a mixed alpha/beta-structure and does not form a transmembrane beta-barrel.

    abstract：:The membrane protein Rv0899 (OmpATb) from Mycobacterium tuberculosis, has been proposed to act as an outer membrane porin and to contribute to the bacterium's adaptation to the acidic environment of the phagosome during infection. The gene is restricted to pathogenic mycobacteria and, thus, is an attractive candidate ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100158s

    authors： Teriete P,Yao Y,Kolodzik A,Yu J,Song H,Niederweis M,Marassi FM

    更新日期：2010-04-06 00:00:00

  • “Locked onto the target”: increasing the efficiency of antagomirzymes using locked nucleic acid modifications.

    abstract：:This study highlights the effect of incorporation of locked nucleic acid (LNA) on improving the functional efficacy of DNAzymes against microRNAs (antagomirzymes). DNAzymes were designed against two different sites of miR-27a, which were encompassed both within the precursor and mature form of miRNA. The cleavage and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101185k

    authors： Kaur H,Scaria V,Maiti S

    更新日期：2010-11-09 00:00:00

  • Iron-sulfur cluster biosynthesis: functional characterization of the N- and C-terminal domains of human NFU.

    abstract：:Human NFU (also known as HIRIP5) has been implicated in cellular iron-sulfur cluster biosynthesis. Bacterial and yeast forms are smaller than the human protein and are homologous to the C-terminal domain of the latter. This C-terminal domain contains a pair of redox active cysteines and demonstrates thioredoxin-like a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801645z

    authors： Liu Y,Qi W,Cowan JA

    更新日期：2009-02-10 00:00:00

  • Codon-induced association of the isolated anticodon loop of tRNAPhe.

    abstract：:The binding of the codon UUC to the isolated anticodon loop of tRNAPhe (yeast) has been studied as a model of codon recognition by a simple adaptor. Fluorescence titrations demonstrate that UUC binds to the isolated anticodon loop with an equilibrium constant of 1.4 X 10(3) M-1 (at 7.2 degrees C). Equilibrium sediment...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00369a005

    authors： Bujalowski W,Jung M,McLaughlin LW,Porschke D

    更新日期：1986-10-21 00:00:00

  • Chromatin-bound protease: degradation of chromosomal proteins under chromatin dissociation conditions.

    abstract：:A chromatin-bound protease, active in 2 M NaCl-5 M urea or 5 M urea alone, was demonstrated in rat liver, kidney, testes, brain, rabbit bone marrow, chicken reticulocyte, and Ehrlich ascites chromatin. Chicken erythrocyte chromatin did not possess any detectable proteolytic activity in salt and urea. The proteolytic a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00646a028

    authors： Carter DB,Chae CB

    更新日期：1976-01-13 00:00:00

  • Anomalous electrophoresis of deoxyribonucleic acid restriction fragments on polyacrylamide gels.

    abstract：:A detailed study has been made of the polyacrylamide gel electrophoresis of DNA restriction fragments obtained from two plasmids, pBR322 and p82-6B. Variables studied were molecular weight, gel concentration, temperature, and electric field strength. The retardation coefficients of the larger fragments (greater than 8...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00295a023

    authors： Stellwagen NC

    更新日期：1983-12-20 00:00:00

  • Selective inactivation of parvulin-like peptidyl-prolyl cis/trans isomerases by juglone.

    abstract：:In contrast to FK506 binding proteins and cyclophilins, the parvulin family of peptidyl-prolyl cis/trans isomerases (PPIases; E.C. 5.2.1.8) cannot be inhibited by either FK506 or cyclosporin A. We have found that juglone, 5-hydroxy-1,4-naphthoquinone, irreversibly inhibits the enzymatic activity of several parvulins, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi973162p

    authors： Hennig L,Christner C,Kipping M,Schelbert B,Rücknagel KP,Grabley S,Küllertz G,Fischer G

    更新日期：1998-04-28 00:00:00

  • Characterizing the response of calcium signal transducers to generated calcium transients.

    abstract：:Cellular Ca2+ transients and Ca2+-binding proteins regulate physiological phenomena as diverse as muscle contraction, neurosecretion, and cell division. When Ca2+ is rapidly mixed with slow Ca2+ chelators, EGTA, or Mg2+/EDTA, artificial Ca2+ transients (ACTs) of varying duration (0.1-50 ms half-widths (hws)) and ampli...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi982495z

    authors： Davis JP,Tikunova SB,Walsh MP,Johnson JD

    更新日期：1999-03-30 00:00:00

  • Simple purification and functional reconstitution of octopus photoreceptor Gq, which couples rhodopsin to phospholipase C.

    abstract：:In invertebrate photoreceptors, illuminated rhodopsin activates multiple G proteins, which are assumed to initiate multiple phototransduction cascades. In this paper, we focused on one of the phototransduction cascades, which utilizes rhodopsin, a Gq-like G protein, and phospholipase C (PLC). A Gq-like G protein from ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961360v

    authors： Kikkawa S,Tominaga K,Nakagawa M,Iwasa T,Tsuda M

    更新日期：1996-12-10 00:00:00

  • Further characterization of the spin coupling observed in oxidized hydrogenase from Chromatium vinosum. A Mössbauer and multifrequency EPR study.

    abstract：:Hydrogenase from Chromatium vinosum contains 1 Ni, 11-12 Fe, and ca. 9 sulfides. EPR and Mössbauer studies of the enzyme prepared in four different oxidation states show that the enzyme contains two Fe4S4 and one Fe3S4 cluster. In the oxidized (2+) state, the Mössbauer parameters of the two Fe4S4 clusters are typical ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00182a029

    authors： Surerus KK,Chen M,van der Zwaan JW,Rusnak FM,Kolk M,Duin EC,Albracht SP,Münck E

    更新日期：1994-04-26 00:00:00