Abstract:
:A chromatin-bound protease, active in 2 M NaCl-5 M urea or 5 M urea alone, was demonstrated in rat liver, kidney, testes, brain, rabbit bone marrow, chicken reticulocyte, and Ehrlich ascites chromatin. Chicken erythrocyte chromatin did not possess any detectable proteolytic activity in salt and urea. The proteolytic activity of rat liver chromatin in salt and urea was found to be independent of the methods of chromatin preparation. The protease can be inhibited by the serine specific reagents phenylmethanesulfonyl fluoride and diisopropyl fluorophosphate and the alkylating reagent, carbobenzoxyphenylalanine chloromethyl ketone, in the presence of organic solvents at 1 mM concentration. The inhibitions of chromatin-bound protease in rat liver by these compounds are irreversible. On the other hand, carbobenzoxyphenylalanine and p-nitrophenyl acetate were shown to be reversible inhibitors of rat liver chromatin-bound protease. The application of these inhibitors during the dissociation of chromatin by salt and urea may be useful to researchers interested in purifying various chromosomal proteins or to those researchers doing reconstitution studies with labile chromatins.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Carter DB,Chae CBdoi
10.1021/bi00646a028subject
Has Abstractpub_date
1976-01-13 00:00:00pages
180-5issue
1eissn
0006-2960issn
1520-4995journal_volume
15pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The cytokinin-active isoprenoid nucleosides of Agrobacterium tumefaciens transfer ribonucleic acid were identified by high-pressure liquid chromatography, permethylation, and mass spectroscopy. Besides the expected 6-[(3-methylbut-2-enyl)amino]-9-(beta-D-ribofuranosyl)purine (i6A) and its 2-methylthio derivative (ms2i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00524a014
更新日期:1981-10-13 00:00:00
abstract::Proton NMR spectroscopy was applied to myoglobin in the ferric, water-liganded form (metMbH2O) and the apo form (apoMb) to probe the structure and stability of the latter. Proteins from sperm whale and horse skeletal muscles were studied to simplify the spectral assignment task. Nuclear Overhauser effects and the resp...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00143a018
更新日期:1992-07-21 00:00:00
abstract::The mammalian plasma vitamin D binding protein (DBP), or Gc-globulin, is recognized to have at least two functional properties: sterol binding and G-actin sequestration. Affinity labeling of the sterol binding site with the radioactive electrophilic ligand, 3 beta-(bromoacetoxy)-25-hydroxycholecalciferol, followed by ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00146a021
更新日期:1992-08-11 00:00:00
abstract::Two intermediates in the biosynthetic pathway to bacterial menaquinones are o-succinylbenzoic acid and 1,4-dihydroxy-2-naphthoic acid. Cell-free extracts have been prepared from Escherichia coli which catalyze the conversion of labeled o-succinylbenzoic acid to the naphthoic acid and also to menaquinones. The naphthoa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00667a007
更新日期:1976-11-02 00:00:00
abstract::Onconase (ONC) from Rana pipiens is the smallest member of the ribonuclease A (RNase A) superfamily. Despite a tertiary structure similar to RNase A, ONC is distinguished by an extremely high thermodynamic stability. In the present paper we have probed the significance of three structural regions, which exhibit struct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0525223
更新日期:2006-03-21 00:00:00
abstract::Recombinant human ferrochelatase has been expressed in Escherichia coli and purified to homogeneity. Metal analyses revealed approximately 2 mol of non-heme Fe per mol of the purified enzyme (M(r) = 40,000). The UV-visible absorption spectrum of the purified enzyme consists of a protein absorption at 278 nm (epsilon a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00168a003
更新日期:1994-01-18 00:00:00
abstract::Unilamellar liposomes can be fused at low pH with the plasma membrane of cells that express the hemagglutinin glycoprotein of influenza virus on their surface [van Meer, G., & Simons, K. (1983) J. Cell Biol. 97, 1365-1374]. Here, we have resolved this fusion process into two kinetically distinct steps. The first and m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00335a030
更新日期:1985-07-02 00:00:00
abstract::The bacterial phosphoenolpyruvate:sugar phosphotransferase system involves a series of reactions in which phosphoprotein intermediates are formed. Histidine-containing protein (HPr) is phosphorylated on the N delta 1 position of the imidazole ring of His15 by enzyme I and acts as a phosphoryl donor to the sugar-specif...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00255a008
更新日期:1994-12-27 00:00:00
abstract::Nitrogenase reduces N2 to NH3, but the mechanistic details are unclear. Diazene (N2H2), a proposed 2e-/2H+ intermediate on the reduction pathway, is labile under typical enzyme assay conditions, and no firm evidence is available on whether or not it can be reduced by or inhibit nitrogenase. In this paper, we compare t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi950964g
更新日期:1996-04-09 00:00:00
abstract::The association of ferredoxin with ferredoxin:NADP+ reductase (both proteins from spinach chloroplasts) was characterized by isothermal titration calorimetry and fluorescence quenching titration. The formation of the complex is mainly driven by a positive entropy change (delta S = 125 +/- 8 J mol-1 K-1). The calorimet...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00249a019
更新日期:1994-11-15 00:00:00
abstract::In the present report we studied the interaction between the skeletal muscle ryanodine receptor and the ubiquitous S100A1 Ca2+ binding protein. S100A1 did not affect equilibrium [3H]ryanodine binding to purified rabbit skeletal muscle terminal cisternae at 100 microM free [Ca2+]. At nanomolar free [Ca2+], however, S10...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970160w
更新日期:1997-09-23 00:00:00
abstract::The structure of isocitrate dehydrogenase (IDH) with a bound complex of isocitrate, NADP+, and Ca2+ was solved at 2.5-A resolution and compared by difference mapping against previously determined enzymatic complexes. Calcium replaces magnesium in the binding of metal-substrate chelate complex, resulting in a substanti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00087a008
更新日期:1993-09-14 00:00:00
abstract::The fosfomycin (1) resistance proteins FosA and FosX in pathogenic microorganisms are related to a catalytically promiscuous progenitor encoded in a phn operon in Mesorhizobium loti. The mlr3345 gene product (FosX(Ml)) from M. loti has a very low epoxide hydrolase activity and even lower glutathione transferase activi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi900078q
更新日期:2009-03-10 00:00:00
abstract::We have sought to determine whether insulin can promote cell survival and protect Chinese hamster ovary (CHO) cells from apoptosis induced by serum starvation. Low concentrations of insulin were antiapoptotic for cells overexpressing wild-type insulin receptors but not in cells transfected with kinase-defective insuli...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9805947
更新日期:1998-11-10 00:00:00
abstract::There is a significant need for new therapeutics to treat infections caused by the biodefense agent Bacillus anthracis. In pursuit of drug discovery against this organism, we have developed novel propargyl-linked inhibitors that target the essential enzyme dihydrofolate reductase (DHFR) from B. anthracis. Previously, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi802319w
更新日期:2009-05-19 00:00:00
abstract::We have modified the cytochrome b subunit of the cytochrome bc1 complex from the purple bacterium Rhodobacter sphaeroides to introduce two distinctive features of cytochrome b6 f complexes. In the first one, we have split cyt b into two polypeptides thus mimicking the organization of cyt b6 and subunit IV in the b6 f ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9813476
更新日期:1998-11-17 00:00:00
abstract::Electron paramagnetic resonance (EPR) imaging utilizing stable nitroxyl radicals is a promising technique for measuring free radical distribution, metabolism, and tissue oxygenation in organs and tissues [Kuppusamy, P., Chzhan, M., Vij, K., Shteynbuk, M., Lefer, D. J., Giannella, E., & Zweier, J. L. (1994) Proc. Natl....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952857s
更新日期:1996-06-04 00:00:00
abstract::Protein phosphatase-1 (PP1) is an essential protein Ser/Thr phosphatase that is extraordinarily conserved from yeast to human, and Inhibitor-2 (I-2) is the most ancient of the heat-stable proteins specific for PP1. We identified novel I-2 homologues in Caenorhabditis elegans (Ce) and Xenopus laevis (Xe) and compared t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi602369m
更新日期:2007-03-06 00:00:00
abstract::Previous studies have shown that the short-motif electroplax Na channel is phosphorylated in vitro by cyclic AMP-dependent protein kinase (PKA) at serines 6 or 7 and 1776 and threonine 17 (Emerick & Agnew, 1989). We here show that phosphatase treatment of solubilized, purified Na channels enhanced subsequent PKA label...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00087a023
更新日期:1993-09-14 00:00:00
abstract::Pulmonary surfactant protein D (SP-D), a member of the collectin group of innate immune proteins, plays important roles in lipopolysaccharide (LPS) recognition. We have previously shown that surfactant protein A (SP-A), a homologous collectin, interacts with Toll-like receptor (TLR) 2, resulting in alteration of TLR2-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060176z
更新日期:2006-07-18 00:00:00
abstract::We studied the interactions between the SH2 domain of growth factor receptor binding protein 2 (Grb2) and ErbB receptor-derived phosphotyrosyl peptides using molecular dynamics, free energy calculations, and surface plasmon resonance (SPR) analysis. Binding free energies for nine phosphotyrosyl peptides were calculate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034113h
更新日期:2003-05-13 00:00:00
abstract::Class I major histocompatibility complex (MHC) proteins present peptide antigens to T cells during the immune response against viruses. Peptides are loaded into newly synthesized class I heterodimers in the endoplasmic reticulum such that most or all cell surface class I molecules contain peptides derived from endogen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00192a020
更新日期:1994-07-05 00:00:00
abstract::The fatty acid acylated insulin, Lys(B29)-tetradecanoyl, des-(B30) human insulin, has been crystallized and the structure determined by X-ray crystallography. The fatty acid substituent on residue B29 Lys binds reversibly to circulating albumin protein in vivo, and by this mechanism the hormone's action is prolonged. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9625105
更新日期:1997-03-11 00:00:00
abstract::A peptide corresponding to a portion of the amphipathic alpha-helical region of CTP:phosphocholine cytidylyltransferase was synthesized. This region of the enzyme was proposed to be the membrane-binding domain [Kalmar, G.B., Kay, R.J., Lachance, A., Aebersold, R., & Cornell, R.B. (1990) Proc. Natl. Acad. Sci. U.S.A. 8...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00180a029
更新日期:1994-04-12 00:00:00
abstract::The side chain of the antifungal polyketide ansatrienin A produced by Streptomyces collinus contains a cyclohexanecarboxylic acid (CHC) derived moiety. This CHC in the coenzyme A activated form (CHC-CoA) is derived from shikimic acid via a pathway in which the penultimate step is the isomerization of 2-cyclohexenylcar...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0005714
更新日期:2000-06-27 00:00:00
abstract::The relative quantitative importance of the factors that determine the fidelity of translation is largely unknown, which makes predicting the extent to which the degeneracy of the genetic code can be broken challenging. Our strategy of using orthogonal tRNA/aminoacyl tRNA synthetase pairs to precisely direct the incor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00177
更新日期:2018-05-15 00:00:00
abstract::Eta-crystallin is a retinal dehydrogenase that has acquired a role as a structural protein in the eye lens of elephant shrews, members of an ancient order of mammals. While it retains some activity toward retinal, which is oxidized to retinoic acid, the protein has acquired a number of specific sequence changes that h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi027367w
更新日期:2003-04-22 00:00:00
abstract::Several recent studies have provided evidence that LIN28, a cytoplasmic RNA-binding protein, inhibits the biogenesis of members of the let-7 microRNA family at the Dicer step in both mammals and Caenorhabditis elegans. However, the precise mechanism of inhibition is still poorly understood. Here we report on an in vit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200851d
更新日期:2011-09-06 00:00:00
abstract::The crystal structure of Pseudomonas putida cytochrome P-450cam in the substrate-free form has been refined at 2.20-A resolution and compared to the substrate-bound form of the enzyme. In the absence of the substrate camphor, the P-450cam heme iron atom is hexacoordinate with the sulfur atom of Cys-357 providing one a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00366a049
更新日期:1986-09-09 00:00:00
abstract::Voltage-dependent anion channel 1 (VDAC1) situated in the outer mitochondrial membrane regulates the transfer of various metabolites and is a key player in mitochondria-mediated apoptosis. Although many small chemicals that modulate the functions of VDAC1 have been reported to date, most, if not all, of them cannot be...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b01209
更新日期:2019-02-26 00:00:00