Distribution of cytokinin-active nucleosides in isoaccepting transfer ribonucleic acids from Agrobacterium tumefaciens.

abstract：:We have developed a panel of antibodies specific for the alpha 1, alpha 2, alpha 3, and beta subunits of the rat Na,K-ATPase. TrpE-alpha subunit isoform fusion proteins were used to generate three antisera, each of which reacted specifically with a distinct alpha subunit isotype. Western blot analysis of rat tissue mi...

journal_title：Biochemistry

authors： Shyjan AW,Levenson R

更新日期：1989-05-30 00:00:00

abstract：:Z-disc-associated, alternatively spliced, PDZ motif-containing protein (ZASP) is a principal component of the sarcomere. The three prevalent isoforms of ZASP in skeletal muscle are generated by alternative splicing of exons 9 and 10. The long isoforms, either having (ZASP-L) or lacking exon 10 (ZASP-LΔex10), include a...

journal_title：Biochemistry

authors： Watts NR,Zhuang X,Kaufman JD,Palmer IW,Dearborn AD,Coscia S,Blech-Hermoni Y,Alfano C,Pastore A,Mankodi A,Wingfield PT

更新日期：2017-04-11 00:00:00

abstract：:Novel inhibitors of lupin diadenosine 5',5'''-P(1),P(4)-tetraphosphate (Ap(4)A) hydrolase have been identified by in silico screening of a large virtual chemical library. Compounds were ranked on the basis of a consensus from six scoring functions. From the top 100 ranked compounds six were selected and initially scre...

journal_title：Biochemistry

authors： Branson KM,Mertens HD,Swarbrick JD,Fletcher JI,Kedzierski L,Gayler KR,Gooley PR,Smith BJ

更新日期：2009-08-18 00:00:00

abstract：:When grown under O2-limited conditions, Escherichia coli expresses a cytochrome bd quinol oxidase that has an unusually high affinity for O2. We have studied the reaction of cytochrome bd with CO and O2 by rapid-reaction spectrophotometry. The reduced enzyme forms a photosensitive ferrocytochrome d-CO complex, and fol...

journal_title：Biochemistry

authors： Hill BC,Hill JJ,Gennis RB

更新日期：1994-12-20 00:00:00

abstract：:The intense intrinsic fluorescence emissions from several clinically relevant camptothecin drugs have been exploited in order to determine (1) the structural basis of drug binding to lipid bilayers, (2) the lipid bilayer stability of each drug's alpha-hydroxylactone moiety, a pharmacophore which is essential for antit...

journal_title：Biochemistry

authors： Burke TG,Mishra AK,Wani MC,Wall ME

更新日期：1993-05-25 00:00:00

abstract：:The human, cytosolic enzyme isocitrate dehydrogenase 1 (IDH1) reversibly converts isocitrate to α-ketoglutarate (αKG). Cancer-associated somatic mutations in IDH1 result in a loss of this normal function but a gain in a new or neomorphic ability to convert αKG to the oncometabolite 2-hydroxyglutarate (2HG). To improve...

journal_title：Biochemistry

authors： Rendina AR,Pietrak B,Smallwood A,Zhao H,Qi H,Quinn C,Adams ND,Concha N,Duraiswami C,Thrall SH,Sweitzer S,Schwartz B

更新日期：2013-07-02 00:00:00

abstract：:Ornithine decarboxylase (ODC) catalyzes the first committed step in the biosynthesis of polyamines, and it has been identified as a drug target for the treatment of African sleeping sickness, caused by Trypanosoma brucei. ODC is a pyridoxal 5'-phosphate (PLP) dependent enzyme and an obligate homodimer. X-ray structura...

journal_title：Biochemistry

authors： Jackson LK,Brooks HB,Osterman AL,Goldsmith EJ,Phillips MA

更新日期：2000-09-19 00:00:00

abstract：:N-Ethylmaleimide modified heavy meromyosin in only 3-fold activated by actin rather than 200-fold as is normal heavy meromyosin (Silverman, R., Eisenberg, E., and Kielley, W. W. (1972), Nature (London) 240, 207). Ultracentrifuge studies demonstrated that in the absence of ATP the N-ethylmaleimide modified heavy merom...

journal_title：Biochemistry

authors： Mulhern S,Eisenberg E,Kielley WW

更新日期：1975-08-26 00:00:00

abstract：:A description of the efficient high-level expression of the monomer hemoglobin (GMG4) from Glycera dibranchiata is presented. The cDNA described by Simons and Satterlee [Simons, P.C., & Satterlee, J.D. (1989) Biochemistry 28, 8525-8530] was subcloned into an expression system, and conditions were found that led to the...

journal_title：Biochemistry

authors： Alam SL,Dutton DP,Satterlee JD

更新日期：1994-08-30 00:00:00

abstract：:We examine the effect of cargo-motor linkage stiffness on the mechanobiological properties of the molecular motor myosin VI. We use the programmability of DNA nanostructures to modulate cargo-motor linkage stiffness and combine it with high-precision optical trapping measurements to measure the effect of linkage stiff...

journal_title：Biochemistry

authors： Shrivastava R,Rai A,Salapaka M,Sivaramakrishnan S

更新日期：2019-11-26 00:00:00

abstract：:The temperature dependence of biological rates at different scales (from individual enzymes to isolated organisms to ecosystem processes such as soil respiration and photosynthesis) is the subject of much historical and contemporary research. The precise relationship between the temperature dependence of enzyme rates ...

journal_title：Biochemistry

authors： Prentice EJ,Hicks J,Ballerstedt H,Blank LM,Liáng LNL,Schipper LA,Arcus VL

更新日期：2020-09-29 00:00:00

abstract：:Protein carboxyl methyltransferases (EC 2.1.1.77) that catalyze the transfer of a methyl group from S-adenosylmethionine to L-isoaspartyl and D-aspartyl residues in a variety of peptides and proteins are widely, but not universally, distributed in nature. These enzymes can participate in the repair of damaged proteins...

journal_title：Biochemistry

authors： Mudgett MB,Clarke S

更新日期：1993-10-19 00:00:00

abstract：:A gentle and rapid isolation procedure is described yielding fractions containing better than 95% pure OmpF porin of Escherichia coli BE with different amounts of bound lipopolysaccharide (LPS). The procedure employs continuous free-flow electrophoresis (FFE) in the presence of detergent above its critical micelle con...

journal_title：Biochemistry

authors： Holzenburg A,Engel A,Kessler R,Manz HJ,Lustig A,Aebi U

更新日期：1989-05-16 00:00:00

abstract：:The interaction between the apoprotein of 6-hydroxy-L-nicotine oxidase from Arthrobacter oxidans and the prosthetic group FAD has been investigated by 13C, 15N, and 31P NMR techniques. The FAD prosthetic group was selectively enriched in 13C and 15N isotopes by adding isotopically labeled riboflavin derivatives to the...

journal_title：Biochemistry

authors： Pust S,Vervoort J,Decker K,Bacher A,Müller F

更新日期：1989-01-24 00:00:00

abstract：:Cellular toxicity resulting from nucleation-dependent polymerization of amyloid beta-peptide (Abeta) is considered to be a major and possibly the primary component of Alzheimer's disease (AD). Inhibition of Abeta polymerization has thus been identified as a target for the development of therapeutic agents for the trea...

journal_title：Biochemistry

authors： Findeis MA,Musso GM,Arico-Muendel CC,Benjamin HW,Hundal AM,Lee JJ,Chin J,Kelley M,Wakefield J,Hayward NJ,Molineaux SM

更新日期：1999-05-25 00:00:00

abstract：:In the presence of Ca2+ and calmodulin (CM), purified smooth muscle myosin light chain kinase (MLCKase) was found to undergo autophosphorylation at a rate that was about 200-fold slower than its catalytic activity. Up to 1.7 mol of phosphate were incorporated per mole of kinase. Lower levels of incorporation could be ...

journal_title：Biochemistry

authors： Sobieszek A

更新日期：1995-09-19 00:00:00

abstract：:EPR studies have revealed that removal of calcium using citric acid from the site in spinach photosystem II which is coupled to the photosynthetic O2-evolving process produces a structural change in the manganese cluster responsible for water oxidation. If done in the dark, this yields a modified S1' oxidation state w...

journal_title：Biochemistry

authors： Sivaraja M,Tso J,Dismukes GC

更新日期：1989-11-28 00:00:00

abstract：:Wideline 2H NMR spectroscopy was used to assess the conformational and orientational effects of N-acetylneuraminic acid (NeuAc) (sialic acid) as a component of a particular oligosaccharide chain at a bilayer membrane surface. For this purpose, three glycosphingolipids, sharing a neutral core tetrasaccharide and differ...

journal_title：Biochemistry

authors： Jones DH,Barber KR,Grant CW

更新日期：1996-04-16 00:00:00

abstract：:Prokaryotic promoters have been extensively studied to relate sequence features to promoter function. Here we examine the relationship between double-helix stability and promoter activity. The double-helix stability is evaluated from sequence data by free energy computation, based on reported values of dinucleotide fr...

journal_title：Biochemistry

authors： Margalit H,Shapiro BA,Nussinov R,Owens J,Jernigan RL

更新日期：1988-07-12 00:00:00

abstract：:Phosphotriesterase homology protein (PHP) is a member of a recently discovered family of proteins related to phosphotriesterase, a hydrolytic, bacterial enzyme with an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates, which are common constituents of chemical warfare agent...

journal_title：Biochemistry

authors： Buchbinder JL,Stephenson RC,Dresser MJ,Pitera JW,Scanlan TS,Fletterick RJ

更新日期：1998-04-14 00:00:00

abstract：:The binding of 125I-labeled ovine follitropin (oFSH) and 125I-labeled deglycosylated ovine follitropin (DG-oFSH) to porcine granulosa cell membranes was studied at equilibrium and nonequilibrium binding conditions and statistically analyzed. Saturation and competition binding experiments revealed homogeneity in the po...

journal_title：Biochemistry

authors： Sebok K,De Lean A,Sairam MR

更新日期：1987-06-16 00:00:00

abstract：:The WSXWS motif is a highly conserved structural feature of the type I cytokine receptor family. It has previously been demonstrated that mutations in the (232)WSAWS(236) motif in the erythropoietin receptor (EPOR) can result in strongly inhibited surface expression, due to defective intracellular transport [Hilton, D...

journal_title：Biochemistry

authors： Furmanek A,Hess D,Rogniaux H,Hofsteenge J

更新日期：2003-07-22 00:00:00

abstract：:The zinc metalloenzyme protein farnesyltransferase (FTase) catalyzes the alkylation of a cysteine residue of protein substrates with a 15 carbon farnesyl group. We have developed fluorescence assays to directly measure the affinity of the enzyme for peptide and protein (Ras) substrates. A peptide corresponding to the ...

journal_title：Biochemistry

authors： Hightower KE,Huang CC,Casey PJ,Fierke CA

更新日期：1998-11-03 00:00:00

abstract：:The oxidation pathway of chlorophyll Z (ChlZ) in photosystem II (PSII) at cryogenic temperatures was studied by means of light-induced Fourier transform infrared (FTIR) difference spectroscopy. To examine the involvement of redox-active beta-carotene (Car) in the pathway, two Car molecules in Mn-depleted PSII membrane...

journal_title：Biochemistry

authors： Kitajima Y,Noguchi T

更新日期：2006-02-14 00:00:00

abstract：:For expression in Saccharomyces cerevisiae the cDNA of the uncoupling protein (UCP) of brown adipose tissue from hamster has been isolated and used to transform yeast cells. Optimized expression conditions yielded 2% of mitochondrial protein as UCP. UCP was isolated, avoiding copurification of ADP/ATP carrier and pori...

journal_title：Biochemistry

authors： Echtay KS,Bienengraeber M,Klingenberg M

更新日期：1997-07-08 00:00:00

abstract：:Human plasma phospholipid transfer protein (PLTP) exchanges phospholipids between lipoproteins and remodels high-density lipoproteins (HDLs). We determined phospholipid transfer activity and HDL binding ability in wild-type PLTP and in 16 PLTP variants created by replacing 12 charged amino acids by site-directed mutag...

journal_title：Biochemistry

authors： Ponsin G,Qu SJ,Fan HZ,Pownall HJ

更新日期：2003-04-22 00:00:00

abstract：:We have mapped the interface between the two transmembrane alpha-helices in the membrane domain of the Escherichia coli enzyme leader peptidase by analyzing disulfides formed between pairs of cysteine residues introduced near their respective periplasmic ends. The interface is formed primarily from aliphatic amino aci...

journal_title：Biochemistry

authors： Whitley P,Nilsson L,von Heijne G

更新日期：1993-08-24 00:00:00

abstract：:The inhibition of phosphorylase kinase catalytic activity by 0.1 M neutral salts was predicted by the Hofmeister series of anions. The site of action of the salts was determined by the following evidence to be on the phosphorylase kinase molecule directly, rather than on its protein substrate. (1) Nonactivated kinase ...

journal_title：Biochemistry

authors： Carlson GM,Graves DJ

更新日期：1976-10-05 00:00:00

abstract：:The immunoglobulin E-binding protein, epsilon BP (also known as CBP35, Mac-2, L-34, and L-29), is a beta-galactoside-binding protein of approximately 30 kDa and a member of the animal lectin family termed S-type or S-Lac. Multiple biological activities have been attributed to this lectin such as mediation of IgE bindi...

journal_title：Biochemistry

authors： Feizi T,Solomon JC,Yuen CT,Jeng KC,Frigeri LG,Hsu DK,Liu FT

更新日期：1994-05-24 00:00:00

abstract：:A soluble extract prepared from cells of an Escherichia coli strain carrying the antibiotic resistance plasmid R6K is capable of carrying out the complete process of R6K DNA replication. DNA synthesis in vitro is dependent on the four deoxyribo- and ribonucleotide triphosphates and is sensitive to rifampin and strepto...

journal_title：Biochemistry

authors： Inuzuka M,Helinski DR

更新日期：1978-06-27 00:00:00