Electron paramagnetic resonance imaging of rat heart with nitroxide and polynitroxyl-albumin.

abstract：:To study the effects of methylation damage on DNA replication in vitro, the plasmid pSVori containing the SV40 origin of replication was reacted with N-methyl-N-nitrosourea and used as a substrate for SV40 T antigen dependent replication by HeLa cell extracts. The plasmid was methylated with a range of N-methyl-N-nitr...

journal_title：Biochemistry

authors： Ceccotti S,Dogliotti E,Gannon J,Karran P,Bignami M

更新日期：1993-12-14 00:00:00

abstract：:To identify residues and segments in the central region of apolipoprotein A-I (apoA-I) that are important for the protein structure and stability, we studied the effects of four double charge ablations, D102A/D103A, E110A/E111A, R116V/K118A, and R160V/H162A, and two deletion mutations, Delta(61-78) and Delta(121-142),...

journal_title：Biochemistry

authors： Gorshkova IN,Liu T,Kan HY,Chroni A,Zannis VI,Atkinson D

更新日期：2006-01-31 00:00:00

abstract：:Steady-state and transient-state analysis of Coprinus cinereus peroxidase, CIP (identical to Arthromyces ramosus peroxidase), was used to characterize the kinetics of the three fundamental steps in heme peroxidase catalysis: compound I (cpd I) formation, cpd I reduction, and compound II (cpd II) reduction. The rate co...

journal_title：Biochemistry

authors： Abelskov AK,Smith AT,Rasmussen CB,Dunford HB,Welinder KG

更新日期：1997-08-05 00:00:00

abstract：:To describe the hydrophobic adsorption of charged molecules to bilayer membranes, one must recognize that the adsorption produces a change in the electrostatic potential at the surface of the membrane. The surface potential produced by the adsorption of the charged molecules can be described most simply by the Gouy eq...

journal_title：Biochemistry

authors： McLaughlin S,Harary H

更新日期：1976-05-04 00:00:00

abstract：:The spin-1/2-carrying NO molecule interacts with both the S1 and S2 states of the water oxidizing complex. The intermediates of the interaction can be resolved and trapped by NO treatment at subzero temperatures. At -30 degrees C and in the presence of approx. 500-700 microM NO, S1 loses the ability to yield by illumi...

journal_title：Biochemistry

authors： Goussias C,Ioannidis N,Petrouleas V

更新日期：1997-07-29 00:00:00

abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins in cyanobacteria and proposed a biosynthetic scheme starting from L-proline and involving three polyketide synthases for which the starte...

journal_title：Biochemistry

authors： Méjean A,Mann S,Vassiliadis G,Lombard B,Loew D,Ploux O

更新日期：2010-01-12 00:00:00

abstract：:The gel to liquid-crystalline phase transition temperatures of dispersions of mixed-acid sn-1,2-lecithins which contain one unsaturated and one saturated fatty acid have been studied by differential scanning calorimetry. The temperature for 1-oleoyl-2-palmitoyl-sn-glycero-3-phosphocholine (containing no reversed isome...

journal_title：Biochemistry

authors： Davis PJ,Fleming BD,Coolbear KP,Keough KM

更新日期：1981-06-09 00:00:00

abstract：:A dark state tertiary structure in the cytoplasmic domain of rhodopsin is presumed to be the key to the restriction of binding of transducin and rhodopsin kinase to rhodopsin. Upon light-activation, this tertiary structure undergoes a conformational change to form a new structure, which is recognized by the above prot...

journal_title：Biochemistry

authors： Klein-Seetharaman J,Hwa J,Cai K,Altenbach C,Hubbell WL,Khorana HG

更新日期：2001-10-23 00:00:00

abstract：:A dimeric bis-benzimidazole molecule has been designed by computer modeling to bind to a DNA sequence via the DNA minor groove that covers a complete turn of B-DNA. A series of bis-benzimidazole dimers incorporating a -O-(CH(2))(n)()-X-CH(2))(n)()-O- linker, with n = 2 or 3 and X = O or N(+)H(Me), were screened for th...

journal_title：Biochemistry

authors： Joubert A,Sun XW,Johansson E,Bailly C,Mann J,Neidle S

更新日期：2003-05-27 00:00:00

abstract：:Proton NMR spectroscopy was applied to myoglobin in the ferric, water-liganded form (metMbH2O) and the apo form (apoMb) to probe the structure and stability of the latter. Proteins from sperm whale and horse skeletal muscles were studied to simplify the spectral assignment task. Nuclear Overhauser effects and the resp...

journal_title：Biochemistry

authors： Cocco MJ,Kao YH,Phillips AT,Lecomte JT

更新日期：1992-07-21 00:00:00

abstract：:αB-Crystallin is a chaperone and an anti-apoptotic protein that is strongly expressed in many tissues, including the lens, retina, heart, and kidney. In the human lens, several lysine residues in αB-crystallin are acetylated. We have previously shown that such acetylation is predominant at lysine 92 (K92) and lysine 1...

journal_title：Biochemistry

authors： Nahomi RB,Huang R,Nandi SK,Wang B,Padmanabha S,Santhoshkumar P,Filipek S,Biswas A,Nagaraj RH

更新日期：2013-11-12 00:00:00

abstract：:The properties of the Fe and Mo sites of the iron-molybdenum cofactor of nitrogenase with respect to binding and activation of N(2) have been studied by molecular mechanics calculations on the local protein environment and by density functional theory (DFT) calculations on subsections of the cofactor. The DFT calculat...

journal_title：Biochemistry

authors： Durrant MC

更新日期：2002-11-26 00:00:00

abstract：:Characterization of the complete gene sequence encoding the alpha-galactosidase from Phanerochaete chrysosporium confirms that this enzyme is a member of glycosyl hydrolase family 27 [Henrissat, B., and Bairoch, A. (1996) Biochem. J. 316, 695-696]. This family, together with the family 36 alpha-galactosidases, forms g...

journal_title：Biochemistry

authors： Hart DO,He S,Chany CJ 2nd,Withers SG,Sims PF,Sinnott ML,Brumer H 3rd

更新日期：2000-08-15 00:00:00

abstract：:Cytochrome P450BM3 is a bacterial enzyme with a heme cofactor that binds small diatomic ligands. Here we report the first study of carbon monoxide (CO) photodissociation and rebinding in ferrous P450BM3 on an ultrafast time scale. We monitored dissociation of carbon monoxide upon Soret band excitation using visible an...

journal_title：Biochemistry

authors： Rupenyan A,Commandeur J,Groot ML

更新日期：2009-07-07 00:00:00

abstract：:A Ca(2+)-activated thiol protease was purified from Drosophila melanogaster. The procedure involves Phenyl-Sepharose, Reactive Red-Agarose and Q-Sepharose fast flow (or MonoQ) chromatographic steps. The enzyme eluting from Q-Sepharose fast flow seems to be homogeneous as judged by silver staining on SDS-PAGE: it consi...

journal_title：Biochemistry

authors： Pintér M,Stierandova A,Friedrich P

更新日期：1992-09-08 00:00:00

abstract：:A likely entry/exit path for nitrogenase substrates, products, and/or protons involves residues α277(Arg), α192(Ser), and α356(Gly), all of which are highly conserved among MoFe proteins from different organisms. The α192(Ser) and α277(Arg) residues form part of a hydrogen-bonded network that also involves α195(His), ...

journal_title：Biochemistry

authors： Fisher K,Hare ND,Newton WE

更新日期：2014-10-07 00:00:00

abstract：:Recombinant human brain calbindin D(28K) (rHCaBP), human Cu,Zn-superoxide dismutase (HCuZnSOD), rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and bovine serum albumin (BSA) were found to be S-glutathiolated in decomposed S-nitrosoglutathione (GSNO) solutions. Tryptic or Glu-C digestion and MALDI-TOF ...

journal_title：Biochemistry

authors： Tao L,English AM

更新日期：2004-04-06 00:00:00

abstract：:We have covalently attached an electron paramagnetic resonance (EPR) spin probe to Cys-670 of the motor domain of ncd (nonclaret disjunctional protein) in order to investigate conformational changes associated with the chemomechanical cycle. Spin-labeling is highly specific and does not affect ncd function as monitore...

journal_title：Biochemistry

authors： Naber N,Cooke R,Pate E

更新日期：1997-08-12 00:00:00

abstract：:Gamma-aminobutyric acid type A receptors (GABA(A) receptors) are ligand-gated chloride channels that play a central role in signal transmission within the mammalian central nervous system. Compounds that modulate specific GABA(A) receptor subtypes containing the delta-subunit are scarce but would be valuable research ...

journal_title：Biochemistry

authors： Lewis RW,Mabry J,Polisar JG,Eagen KP,Ganem B,Hess GP

更新日期：2010-06-15 00:00:00

abstract：:The inflammatory mediator nitric oxide (NO*) promotes apoptotic cell death based on morphological evidence, accumulation of the tumor suppressor p53, caspase-3 activation, and DNA fragmentation in RAW 264.7 macrophages. Since nitrosothiols may actually be the predominant form of biologically active NO* in vivo, we use...

journal_title：Biochemistry

authors： Callsen D,Brüne B

更新日期：1999-02-23 00:00:00

abstract：:A considerable body of data supports the model that the infectious agent (called a prion) which causes the transmissible spongiform encephalopathies is a replicating polypeptide devoid of nucleic acid. Prions are believed to propagate by changing the conformation of the normal cellular prion protein (PrPc) into an inf...

journal_title：Biochemistry

authors： Cappai R,Stewart L,Jobling MF,Thyer JM,White AR,Beyreuther K,Collins SJ,Masters CL,Barrow CJ

更新日期：1999-03-16 00:00:00

abstract：:Cyclic AMP receptor protein is involved in the regulation of more than 20 genes. A step in the mechanism of activation of transcription is to induce a significant bending of the DNA upon complex formation between specific DNA and the protein. The induced DNA bending and a structure of the protein-DNA complex were stud...

journal_title：Biochemistry

authors： Heyduk T,Lee JC

更新日期：1992-06-09 00:00:00

abstract：:We have analyzed by band-shift assays HeLa cell protein-DNA interactions on a stable cruciform DNA molecule. The stable cruciform was formed by heteroduplexing the HindIII-SphI fragment of SV40 virus DNA that contains the origin of replication with a derivative mutant containing a heterologous substitution at the cent...

journal_title：Biochemistry

authors： Pearson CE,Ruiz MT,Price GB,Zannis-Hadjopoulos M

更新日期：1994-11-29 00:00:00

abstract：:In an earlier study, β³-puromycin was used for the selection of modified ribosomes, which were utilized for the incorporation of five different β-amino acids into Escherichia coli dihydrofolate reductase (DHFR). The selected ribosomes were able to incorporate structurally disparate β-amino acids into DHFR, in spite of...

journal_title：Biochemistry

authors： Maini R,Chowdhury SR,Dedkova LM,Roy B,Daskalova SM,Paul R,Chen S,Hecht SM

更新日期：2015-06-16 00:00:00

abstract：:Streptomyces griseus trypsin has been isolated from Pronase by ion-exchange chromatography on CM-Sephadex and SE-Sephadex. The isolated enzyme was homogeneous by the criteria tested except for a low degree of contamination by an enzyme with nontryptic activity. The latter could be partially resolved by chromatography ...

journal_title：Biochemistry

authors： Olafson RW,Smillie LB

更新日期：1975-03-25 00:00:00

abstract：:We have prepared the following cadmium-113-substituted derivatives of bovine superoxide dismutase and recorded the nuclear magnetic resonance (NMR) spectrum of the cadmium: 2Cd(II), in which Cd(II) is presumed to bind to the Zn(II) site and the copper site is unoccupied, and 2Cd(II)--2Cu(I), which is analogous to the ...

journal_title：Biochemistry

authors： Bailey DB,Ellis PD,Fee JA

更新日期：1980-02-05 00:00:00

abstract：:Phenylcarbene and adamantylidene have been generated photochemically from the corresponding diazirines within lipid bilayers. Reasonable yields of labeled fatty acid side chains have been observed. The products have been characterized by gas chromatography-mass spectrometry and derive both from the insertion of the c...

journal_title：Biochemistry

authors： Bayley H,Knowles JR

更新日期：1978-06-13 00:00:00

abstract：:Pulmonary surfactant protein D (SP-D), a member of the collectin group of innate immune proteins, plays important roles in lipopolysaccharide (LPS) recognition. We have previously shown that surfactant protein A (SP-A), a homologous collectin, interacts with Toll-like receptor (TLR) 2, resulting in alteration of TLR2-...

journal_title：Biochemistry

authors： Ohya M,Nishitani C,Sano H,Yamada C,Mitsuzawa H,Shimizu T,Saito T,Smith K,Crouch E,Kuroki Y

更新日期：2006-07-18 00:00:00

abstract：:The rapid phosphorylation and dephosphorylation of a variety of proteins downstream of the endothelin receptors A and B was investigated following stimulation of human lung fibroblasts with endothelin-1. Changes in the phosphorylation of proteins involved in the cell cycle, cytoskeleton, membrane channels, transcripti...

journal_title：Biochemistry

authors： Stannard C,Soskic V,Godovac-Zimmermann J

更新日期：2003-12-02 00:00:00

abstract：:Prokaryotic promoters have been extensively studied to relate sequence features to promoter function. Here we examine the relationship between double-helix stability and promoter activity. The double-helix stability is evaluated from sequence data by free energy computation, based on reported values of dinucleotide fr...

journal_title：Biochemistry

authors： Margalit H,Shapiro BA,Nussinov R,Owens J,Jernigan RL

更新日期：1988-07-12 00:00:00