Structure and stability of apolipoprotein a-I in solution and in discoidal high-density lipoprotein probed by double charge ablation and deletion mutation.

Abstract:

:To identify residues and segments in the central region of apolipoprotein A-I (apoA-I) that are important for the protein structure and stability, we studied the effects of four double charge ablations, D102A/D103A, E110A/E111A, R116V/K118A, and R160V/H162A, and two deletion mutations, Delta(61-78) and Delta(121-142), on the conformation and stability of apoA-I in the lipid-free state and in reconstituted discoidal phospholipid-cholesterol-apoA-I particles (rHDL). The findings suggest that D102/D103 and E110/E111 located in helix 4 and segment(s) between residues 61 and 78 are involved in maintenance of the conformation and stability of apoA-I in both the lipid-free state and in rHDL. R116/K118 located in helix 4 are essential for the conformation and stabilization of apoA-I in rHDL but not vital for the lipid-free state of the protein. The R160V/H162A substitutions in helix 6 lead to a less compact tertiary structure of lipid-free apoA-I without notable effects on the lipid-free or lipid-bound secondary conformation, suggesting involvement of R160/H162 in important interhelical interactions. The results on the Delta(121-142) mutant, together with our earlier findings, suggest disordered structure of a major segment between residues 121 and 143, likely including residues 131-143, in lipid-free apoA-I. Our findings provide the first experimental evidence for stabilization of rHDL by specific electrostatic interhelical interactions, in agreement with the double belt model. The effects of alterations in the conformation and stability of the apoA-I mutants on in vitro and in vivo functions of apoA-I and lipid homeostasis are discussed.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Gorshkova IN,Liu T,Kan HY,Chroni A,Zannis VI,Atkinson D

doi

10.1021/bi051669r

subject

Has Abstract

pub_date

2006-01-31 00:00:00

pages

1242-54

issue

4

eissn

0006-2960

issn

1520-4995

journal_volume

45

pub_type

杂志文章
  • Amyloid nucleation triggered by agitation of beta2-microglobulin under acidic and neutral pH conditions.

    abstract::Amyloid nucleation through agitation was studied with beta2-microglobulin, which is responsible for dialysis-related amyloidosis, in the presence of salt under acid and neutral pH conditions. First, the aggregation of beta2-microglobulin in NaCl solutions was achieved by mildly agitating for 24 h at 37 degrees C prote...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi701968g

    authors: Sasahara K,Yagi H,Sakai M,Naiki H,Goto Y

    更新日期:2008-02-26 00:00:00

  • The CD4 determinant for downregulation by HIV-1 Nef directly binds to Nef. Mapping of the Nef binding surface by NMR.

    abstract::Using heteronuclear NMR spectroscopy, we demonstrate that a 13-residue peptide (MS-QIKRLLSEKKT) from the cytoplasmic tail of CD4 binds to Nef protein. This part of CD4 is critical for downregulation of CD4 by HIV-1 Nef [Aiken et al. (1994) Cell 76, 853-864]. We show that a control peptide without the central dileucine...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9611164

    authors: Grzesiek S,Stahl SJ,Wingfield PT,Bax A

    更新日期:1996-08-13 00:00:00

  • Role of the sulfonium center in determining the ligand specificity of human s-adenosylmethionine decarboxylase.

    abstract::S-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the polyamine biosynthetic pathway. Inhibition of this pathway and subsequent depletion of polyamine levels is a viable strategy for cancer chemotherapy and for the treatment of parasitic diseases. Substrate analogue inhibitors display an absolute requir...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi900590m

    authors: Bale S,Brooks W,Hanes JW,Mahesan AM,Guida WC,Ealick SE

    更新日期:2009-07-14 00:00:00

  • Translocation of E. coli RecQ helicase on single-stranded DNA.

    abstract::A member of the SF2 family of helicases, Escherichia coli RecQ, is involved in the recombination and repair of double-stranded DNA breaks and single-stranded DNA (ssDNA) gaps. Although the unwinding activity of this helicase has been studied biochemically, the mechanism of translocation remains unclear. To this end, u...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi3000676

    authors: Rad B,Kowalczykowski SC

    更新日期:2012-04-03 00:00:00

  • Analysis of amino and carboxy terminal GLUT-4 targeting motifs in 3T3-L1 adipocytes using an endosomal ablation technique.

    abstract::The targeting of the insulin-responsive glucose transporter, GLUT-4, to an intracellular compartment in adipocytes and muscle is one of the key features responsible for the unique insulin sensitivity of this transporter. Through expression of epitope-tagged GLUT-4 mutants in 3T3-L1 adipocytes, two motifs have been ide...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi980988y

    authors: Melvin DR,Marsh BJ,Walmsley AR,James DE,Gould GW

    更新日期:1999-02-02 00:00:00

  • An unusual pattern of CytR and CRP binding energetics at Escherichia coli cddP suggests a unique blend of class I and class II mediated activation.

    abstract::Two transcription factors, CRP and CytR, mediate positive and negative control of nine cistrons involved in nucleoside catabolism and recycling in Escherichia coli. The ability of multiple transcription factors to combine in different ways to confer differential gene regulation is of significant interest in both proka...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi901583n

    authors: Holt AK,Senear DF

    更新日期:2010-01-26 00:00:00

  • Binding of polyaminocarboxylate chelators to the active-site copper inhibits the GSNO-reductase activity but not the superoxide dismutase activity of Cu,Zn-superoxide dismutase.

    abstract::In addition to its superoxide dismutase (SOD) activity, Cu,Zn-superoxide dismutase (CuZnSOD) catalyzes the reductive decomposition of S-nitroso-L-glutathione (GSNO) in the presence of thiols such as L-glutathione (GSH). The GSNO-reductase activity but not the superoxide dismutase (SOD) activity of CuZnSOD is inhibited...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi061077o

    authors: Ye M,English AM

    更新日期:2006-10-24 00:00:00

  • Purification, characterization, and substrate and inhibitor structure-activity studies of rat liver FAD-AMP lyase (cyclizing): preference for FAD and specificity for splitting ribonucleoside diphosphate-X into ribonucleotide and a five-atom cyclic phospho

    abstract::An enzyme with FAD-AMP lyase (cyclizing) activity, splitting FAD to AMP and riboflavin 4',5'-phosphate (cFMN), was recently identified [Fraiz, F., et al. (1998) Biochem. J. 330, 881-888]. Now, it has been purified to apparent homogeneity from a rat liver supernatant, by a procedure that includes affinity for ADP-agaro...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0157159

    authors: Cabezas A,Pinto RM,Fraiz F,Canales J,González-Santiago S,Cameselle JC

    更新日期:2001-11-13 00:00:00

  • Comparative Analysis of CPI-Motif Regulation of Biochemical Functions of Actin Capping Protein.

    abstract::The heterodimeric actin capping protein (CP) is regulated by a set of proteins that contain CP-interacting (CPI) motifs. Outside of the CPI motif, the sequences of these proteins are unrelated and distinct. The CPI motif and surrounding sequences are conserved within a given protein family, when compared to those of o...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.0c00092

    authors: McConnell P,Mekel M,Kozlov AG,Mooren OL,Lohman TM,Cooper JA

    更新日期:2020-03-24 00:00:00

  • Addition of lysines to the 50/20 kDa junction of myosin strengthens weak binding to actin without affecting the maximum ATPase activity.

    abstract::Much interest has centered on two surface loops in the motor domain to explain the differences in enzymatic and mechanical properties of myosin isoforms. We showed that two invariant lysines at the C-terminal end of loop 2, which is part of the actin-binding interface, are required to obtain actin activation [Joel et ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi034415j

    authors: Joel PB,Sweeney HL,Trybus KM

    更新日期:2003-08-05 00:00:00

  • Structural and functional analysis of the homing endonuclease PI-sceI by limited proteolytic cleavage and molecular cloning of partial digestion products.

    abstract::PI-SceI is a member of an unusual class of rare cutting homing endonucleases produced by an autocatalytic protein splicing from a precursor. To analyze the structural and functional domain organization of the endonuclease PI-SceI and to examine whether the DNA binding activity can be structurally separated from the ca...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi980013d

    authors: Pingoud V,Grindl W,Wende W,Thole H,Pingoud A

    更新日期:1998-06-02 00:00:00

  • Thermodynamics of bovine spleen galectin-1 binding to disaccharides: correlation with structure and its effect on oligomerization at the denaturation temperature.

    abstract::Isothermal titration calorimetry (ITC) measurements of the binding 1-beta carbohydrate-substituted galactopyranoside derivatives to galectin-1 from bovine spleen, a dimer with one binding site per subunit, were performed at 283-285 and 298 K. The disaccharides were lactose, methyl beta-lactoside, lactulose, 4-O-beta-D...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9716478

    authors: Schwarz FP,Ahmed H,Bianchet MA,Amzel LM,Vasta GR

    更新日期:1998-04-28 00:00:00

  • Interaction of the pore-forming domain of colicin A with phospholipid vesicles.

    abstract::The interaction of the 20-kDa pore-forming domain of colicin A with phospholipid vesicles was investigated by gel permeation chromatography, analytical centrifugation, and electron microscopy. Under the experimental conditions of this study, this peptide was found to interact only with vesicles containing negatively c...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00445a029

    authors: Massotte D,Dasseux JL,Sauve P,Cyrklaff M,Leonard K,Pattus F

    更新日期:1989-09-19 00:00:00

  • Sugar recognition by CscB and LacY.

    abstract::The sucrose permease (CscB) and lactose permease (LacY) of Escherichia coli belong to the oligosaccharide/H(+) symporter subfamily of the major facilitator superfamily, and both catalyze sugar/H(+) symport across the cytoplasmic membrane. Thus far, there is no common substrate for the two permeases; CscB transports su...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi201592y

    authors: Sugihara J,Smirnova I,Kasho V,Kaback HR

    更新日期:2011-12-27 00:00:00

  • Prothrombin domains: circular dichroic evidence for a lack of cooperativity.

    abstract::The far-ultraviolet circular dichroism spectra of bovine and human prothrombin, prothrombin fragment 1, prethrombin 1, prothrombin fragment 2, and prethrombin 2 (prethrombin 2des(1--13)) were determined and the method of Chen et al. [Chen, Y. H., Yang, J. T., & Martinez, H. M. (1972) Biochemistry 11, 4120--4131; Chen,...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00577a017

    authors: Bloom JW,Mann KG

    更新日期:1979-05-15 00:00:00

  • Selecting for Fast Protein-Protein Association As Demonstrated on a Random TEM1 Yeast Library Binding BLIP.

    abstract::Protein-protein interactions mediate the vast majority of cellular processes. Though protein interactions obey basic chemical principles also within the cell, the in vivo physiological environment may not allow for equilibrium to be reached. Thus, in vitro measured thermodynamic affinity may not provide a complete pic...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.8b00172

    authors: Cohen-Khait R,Schreiber G

    更新日期:2018-08-07 00:00:00

  • Operative Binding of Class I Release Factors and YaeJ Stabilizes the Ribosome in the Nonrotated State.

    abstract::During translation, the small subunit of the ribosome rotates with respect to the large subunit primarily between two states as mRNA is being translated into a protein. At the termination of bacterial translation, class I release factors (RFs) bind to a stop codon in the A-site and catalyze the release of the peptide ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.7b00824

    authors: Casy W,Prater AR,Cornish PV

    更新日期:2018-04-03 00:00:00

  • Structural analysis of sterol distributions in the plasma membrane of living cells.

    abstract::Although plasma membrane (PM) cholesterol-rich and -poor domains have been isolated by subcellular fractionation, the real-time arrangement of cholesterol in such domains in living cells is still unclear. Therefore, dehydroergosterol (DHE), a naturally occurring fluorescent sterol, was incorporated into cultured L-cel...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi048172m

    authors: Zhang W,McIntosh AL,Xu H,Wu D,Gruninger T,Atshaves B,Liu JC,Schroeder F

    更新日期:2005-03-01 00:00:00

  • Mycobacterium tuberculosis Rv0899 adopts a mixed alpha/beta-structure and does not form a transmembrane beta-barrel.

    abstract::The membrane protein Rv0899 (OmpATb) from Mycobacterium tuberculosis, has been proposed to act as an outer membrane porin and to contribute to the bacterium's adaptation to the acidic environment of the phagosome during infection. The gene is restricted to pathogenic mycobacteria and, thus, is an attractive candidate ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi100158s

    authors: Teriete P,Yao Y,Kolodzik A,Yu J,Song H,Niederweis M,Marassi FM

    更新日期:2010-04-06 00:00:00

  • Targeted cross-linking of a molten globule form of acetylcholinesterase by the virucidal agent hypericin.

    abstract::The natural product hypericin is a photosensitive polycyclic aromatic dione compound, which has been widely investigated because of its virucidal and antitumor properties. Although it has been suggested that singlet oxygen or a radical species might be responsible for its biological action, its mechanism of action rem...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi991147+

    authors: Weiner L,Roth E,Mazur Y,Silman I

    更新日期:1999-08-31 00:00:00

  • The 2.7 A crystal structure of the activated FERM domain of moesin: an analysis of structural changes on activation.

    abstract::Moesin binds to a large range of proteins through its N terminal FERM (band 4.1, ezrin, radixin, moesin) domain. In full-length moesin isolated from cells, this binding is masked by binding to the C-terminal domain of moesin (C-ERMAD). Activation takes place by phosphorylation of Thr 558 in the C-ERMAD, which releases...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi010419h

    authors: Edwards SD,Keep NH

    更新日期:2001-06-19 00:00:00

  • Reactions of the sarcoplasmic reticulum calcium adenosinetriphosphatase with adenosine 5'-triphosphate and Ca2+ that are not satisfactorily described by an E1-E2 model.

    abstract::Phosphorylation of the sarcoplasmic reticulum calcium ATPase, E, is first order with kb = 70 +/- 7 s-1 after free enzyme was mixed with saturating ATP and 50 microM Ca2+; this is one-third the rate constant of 220 s-1 for phosphorylation of enzyme preincubated with calcium, cE.Ca2, after being mixed with ATP under the...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00398a019

    authors: Stahl N,Jencks WP

    更新日期:1987-12-01 00:00:00

  • Mechanism of oligonucleotide loop formation in solution.

    abstract::We have studied the tridecadeoxynucleotide CGCGAATTACGCG (I), which contains an additional A at position 9 compared to the dodecanucleotide of which the crystal structure has been determined. Sequence I exhibits no distinct melting curve and also has a concentration-dependent pattern of peaks on reverse-phase chromato...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00371a025

    authors: Roy S,Weinstein S,Borah B,Nickol J,Appella E,Sussman JL,Miller M,Shindo H,Cohen JS

    更新日期:1986-11-18 00:00:00

  • Structure-sensitive RNA footprinting of yeast nuclear ribonuclease P.

    abstract::Several enzymatic and chemical reagents were used to probe the secondary structure of Saccharomyces cerevisiae nuclear RNase P RNA in the presence and absence of its protein components. Double-stranded regions were detected with RNase V1 and single-stranded regions with RNase ONE (Escherichia coli RNase I). Nucleotide...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00173a022

    authors: Tranguch AJ,Kindelberger DW,Rohlman CE,Lee JY,Engelke DR

    更新日期:1994-02-22 00:00:00

  • Sequence of a rabbit anti-Micrococcus lysodeikticus antibody light chain.

    abstract::The complete sequence of rabbit antibody light-chain L 120 has been elucidated. The antibody was raised against Micrococcus lysodeikticus bacteria and is specific for the external part of the cell wall. All protein used in this work was obtained from a single 50-mL bleeding. The variable region of L 120 is compared to...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00613a031

    authors: Van Hoegaerden M,Strosberg AD

    更新日期:1978-10-03 00:00:00

  • Light-induced membrane potential and pH gradient in Halobacterium halobium envelope vesicles.

    abstract::Illumination of envelope vesicles prepared from Halobacterium halobium cells causes translocation of protons from inside to outside, due to the light-induced cycling of bacteriorhodopsin. This process results in a pH gradient across the membranes, an electrical potential, and the movements of K+ and Na+. The electrica...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00655a017

    authors: Renthal R,Lanyi JK

    更新日期:1976-05-18 00:00:00

  • Using saturation-recovery EPR to measure distances in proteins: applications to photosystem II.

    abstract::The stable tyrosine radical YD. (tyrosine 160 in the D2 polypeptide) in photosystem II (PSII) exhibits nonexponential electron spin-lattice relaxation transients at low temperature. As previously reported, the tetranuclear Mn complex in PSII significantly enhances the spin-lattice relaxation of YD.. However, in Mn-dep...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00117a033

    authors: Hirsh DJ,Beck WF,Innes JB,Brudvig GW

    更新日期:1992-01-21 00:00:00

  • In vitro conversion of formate to serine: effect of tetrahydropteroylpolyglutamates and serine hydroxymethyltransferase on the rate of 10-formyltetrahydrofolate synthetase.

    abstract::Serine hydroxymethyltransferase and C1-tetrahydrofolate synthase catalyze four reactions which convert formate and glycine to serine. The one-carbon carrier in these reactions if tetrahydropteroylglutamate which is regenerated in the coupled reaction and thus can be used in catalytic concentrations with respect to ser...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00450a028

    authors: Strong WB,Schirch V

    更新日期:1989-11-28 00:00:00

  • Evidence for the kinetic partitioning of polymerase activity on G-quadruplex DNA.

    abstract::We have investigated the action of the human DNA polymerase ε (hpol ε) and η (hpol η) catalytic cores on G-quadruplex (G4) DNA substrates derived from the promoter of the c-MYC proto-oncogene. The translesion enzyme hpol η exhibits a 6.2-fold preference for binding to G4 DNA over non-G4 DNA, while hpol ε binds both G4...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.5b00060

    authors: Eddy S,Maddukuri L,Ketkar A,Zafar MK,Henninger EE,Pursell ZF,Eoff RL

    更新日期:2015-05-26 00:00:00

  • Functions of the 5'-phosphoryl group of pyridoxal 5'-phosphate in phosphorylase: a study using pyridoxal-reconstituted enzyme as a model system.

    abstract::Pyridoxal-reconstituted phosphorylase was used as a model system to study the possible functions of the 5'-phosphoryl group of pyridoxal 5'-phosphate (PLP) in rabbit muscle glycogen phosphorylase. Kinetic study was conducted by using competitive inhibitors of phosphite, an activator, and alpha-D-glucopyranose 1-phosph...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00290a017

    authors: Chang YC,McCalmont T,Graves DJ

    更新日期:1983-10-11 00:00:00