Abstract：
:Recombinant human brain calbindin D(28K) (rHCaBP), human Cu,Zn-superoxide dismutase (HCuZnSOD), rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and bovine serum albumin (BSA) were found to be S-glutathiolated in decomposed S-nitrosoglutathione (GSNO) solutions. Tryptic or Glu-C digestion and MALDI-TOF MS analyses of the digests are consistent with S-thiolation of Cys111 and Cys187 of HCuZnSOD and rHCaBP, respectively, upon exposure to decomposed GSNO. GAPDH activity analysis reveals that S-glutathiolation most likely occurs on the active site Cys149, and the single free Cys34 is assumed to be the site of S-glutathiolation in BSA. The yields of S-glutathiolation of rHCaBP, GAPDH, and BSA were much higher than those of HCuZnSOD. The latter is limited by the accessibility of Cys111 to the glutathiolating reagent in the HCuZnSOD dimer. Unlike decomposed GSNO, fresh GSNO, reduced glutathione (GSH), and oxidized glutathione (GSSG) are not efficient S-glutathiolating agents for the proteins examined here. On the basis of analysis by mass spectrometry and UV-visible absorption, GSNO decomposition in the dark at room temperature yields glutathione disulfide S-oxide [GS(O)SG], glutathione disulfide S-dioxide (GSO(2)SG), and GSSG as products. GS(O)SG is the efficient protein S-glutathiolating agent in GSNO solutions, not GSNO, which does not carry out efficient S-glutathiolation of rHCaBP, HCuZnSOD, or GAPDH in vitro. A hydrolysis pathway yielding GSOH and nitroxyl (HNO/NO(-)) as intermediates is proposed for GSNO decomposition in the dark. This is based on inhibition of GSNO breakdown by dimedone, a reagent specific for sulfenic acids, and on nitroxyl scavenging by metmyoglobin. The results presented here are contrary to numerous reports of protein S-thiolation by low-molecular weight S-nitrosothiols.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Tao L,English AMdoi
10.1021/bi035924osubject
Has Abstractpub_date
2004-04-06 00:00:00pages
4028-38issue
13eissn
0006-2960issn
1520-4995journal_volume
43pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The 18 kDa translocator protein (TSPO), formerly known as the peripheral-type benzodiazepine receptor, has been reported to be closely associated with the mitochondrial permeability transition pore (MPTP). TSPO is believed to exert pro-apoptotic functions via modulation of MPTP opening. Cobalt chloride (CoCl(2)), whic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900064t
更新日期：2009-06-02 00:00:00
abstract：:There are 10 transmembrane protein (TMEM) 16-family proteins in humans and mice. Among them, TMEM16F acts as a Ca(2+)-dependent phospholipid scramblase at the plasma membrane. However, how Ca(2+) activates TMEM16F's phospholipid-scramblase activity has not been elucidated. Here we found that in the presence of Ca(2+),...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00176
更新日期：2016-06-14 00:00:00
abstract：:Eosinophil cationic protein (ECP) is a component of the eosinophil granule matrix. It shows marked toxicity against helminth parasites, bacteria single-stranded RNA viruses, and host epithelial cells. Secretion of human ECP is related to eosinophil-associated allergic, asthmatic, and inflammatory diseases. ECP belongs...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0264521
更新日期：2002-10-08 00:00:00
abstract：:In all structurally characterized bacterial multicomponent monooxygenase (BMM) hydroxylase proteins, a series of hydrophobic cavities in the α-subunit trace a conserved path from the protein exterior to the carboxylate-bridged diiron active site. This study examines these cavities as a potential route for transport of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201248b
更新日期：2011-12-27 00:00:00
abstract：:A nine-base oligodeoxyribonucleotide complementary to bases 2497-2505 of 23S rRNA was hybridized to both 50S subunits and 70S ribosomes. The binding of the probe to the ribosome or ribosomal subunits was assayed by nitrocellulose filtration and by sucrose gradient centrifugation techniques. The location of the hybridi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00428a073
更新日期：1989-01-24 00:00:00
abstract：:Hypoxic respiratory diseases or hypoxia exposures are frequently accompanied by glucose intolerance and impaired nitric oxide (NO) availability. However, the molecular mechanism responsible for impaired NO production and insulin resistance (IR) during hypoxia remains obscure. In this study, we investigated the possibl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401061e
更新日期：2014-01-14 00:00:00
abstract：:Sorcin, a 21.6 kDa cytosolic EF-hand protein which undergoes a Ca(2+)-induced translocation from cytoplasm to membranes, has been assigned to the newly defined penta EF-hand family. A molecular model of the C-terminal Ca(2+)-binding domain has been generated using as a template the X-ray coordinates of the correspondi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991648v
更新日期：2000-02-01 00:00:00
abstract：:Paranemic crossover DNA (PX-DNA) is a four-stranded multicrossover structure that has been implicated in recombination-independent recognition of homology. Although existing evidence has suggested that PX is the DNA motif in homologous pairing (HP), this conclusion remains ambiguous. Further investigation is needed bu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00057
更新日期：2019-03-12 00:00:00
abstract：:Three sites of N(G),N(G)-arginine methylation have been located at residues 205, 217, and 224 in the glycine-rich, COOH-terminal one-third of the HeLa A1 heterogeneous ribonucleoprotein. Together with the previously determined dimethylated arginine at position 193 [Williams et al., (1985) Proc. Natl. Acad. Sci. U.S.A....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9625509
更新日期：1997-04-29 00:00:00
abstract：:The Radical SAM (RS) enzyme PqqE catalyzes the first step in the biosynthesis of the bacterial cofactor pyrroloquinoline quinone, forming a new carbon-carbon bond between two side chains within the ribosomally synthesized peptide substrate PqqA. In addition to the active site RS 4Fe-4S cluster, PqqE is predicted to ha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b01097
更新日期：2018-02-27 00:00:00
abstract：:Lamin A protein, encoded by the LMNA gene, belongs to the type V intermediate filament protein family and is a major nuclear protein component of higher metazoan organisms, including humans. Lamin A along with B-type lamins impart structural rigidity to the nucleus by forming a lamina that is closely apposed to the in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400337t
更新日期：2013-06-18 00:00:00
abstract：:UreE is a homodimeric metallo-chaperone that assists the insertion of Ni(2+) ions in the active site of urease. The crystal structures of UreE from Bacillus pasteurii and Klebsiella aerogenes have been determined, but the details of the nickel-binding site were not elucidated due to solid-state effects that caused dis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0601003
更新日期：2006-05-23 00:00:00
abstract：:Amino acid selective isotope labeling is an important nuclear magnetic resonance technique, especially for larger proteins, providing strong bases for the unambiguous resonance assignments and information concerning the structure, dynamics, and intermolecular interactions. Amino acid selective 15N labeling suffers fro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00182
更新日期：2018-07-03 00:00:00
abstract：:Pepstatin is a slow and tight-binding inhibitor of pepsin. Preincubating enzyme and inhibitor in H2O and in D2O in the absence of substrate generates an inverse solvent isotope effect of Dk = 0.69 +/- 0.06 on the apparent first-order rate constant for the decay in enzymatic activity. Proton inventory analysis of the i...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00198a032
更新日期：1994-08-16 00:00:00
abstract：:The reaction of nitric oxide (NO) with fast cytochrome bo from Escherichia coli has been studied by electronic absorption, MCD, and EPR spectroscopy. Titration of the enzyme with NO showed the formation of two distinct species, consistent with NO binding stoichiometries of 1:1 and 2:1 with observed dissociation consta...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971481a
更新日期：1997-12-23 00:00:00
abstract：:The interaction of pig plasma gelsolin (G) and actin (A) was examined by using photoreactive 4-maleimidobenzophenone-actin (BPM-actin) in which BPM was previously conjugated to Cys-374 of actin through the maleimide moiety. In the presence of micromolar [Ca2+], the major cross-linked product observed after irradiation...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00237a020
更新日期：1991-06-11 00:00:00
abstract：:G-Rich DNA and RNA have a higher propensity to form G-quadruplex structures, but the presence of G-runs alone is not sufficient to prove that such sequences can form stable G-quadruplexes. While G-rich sequences are essential for G-quadruplex formation, not all G-rich sequences have the propensity to form G-quadruplex...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500773c
更新日期：2014-11-18 00:00:00
abstract：:The Escherichia coli siderophore enterobactin is assembled from 2,3-dihydroxybenzoate (2,3-DHB) and l-serine by the nonribosomal peptide synthetases EntB and EntF. The processive thiol-template strategy used can be sabotaged by EntB misacylation. Through in vitro kinetic analysis, we demonstrate two potential routes t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi802207t
更新日期：2009-01-27 00:00:00
abstract：:Methenyltetrahydromethanopterin (methenyl-H(4)MPT(+)) cyclohydrolase (Mch) catalyzes the interconversion of methenyl-H(4)MPT(+) and formyl-H(4)MPT in the one-carbon energy metabolism of methanogenic, methanotrophic, and sulfate-reducing archaea and of methylotrophic bacteria. To understand the catalytic mechanism of t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300777k
更新日期：2012-10-23 00:00:00
abstract：:Measurement of x-ray scattering at very small angles by solutions of myosin subfragment 1 (S1) yields a radius of gyration of 3.24 nm (mean) +/- 0.03 nm (standard error for N = 9). If S1 is assumed to be ellipsoid of revolution, of uniform electron density, with a molecular weight of 1.15 X 10(5) and a partial specifi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00605a009
更新日期：1978-06-13 00:00:00
abstract：:The crystal structure of the catalytic domain of rat DNA polymerase beta revealed that Asp256 is located in proximity to the previously identified active site residues Asp190 and Asp192. We have prepared and kinetically characterized the nucleotidyl transfer activity of wild type and several mutant forms of human and ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00049a008
更新日期：1995-12-12 00:00:00
abstract：:We have purified and characterized a novel 30-kDa glycoprotein (gp30) with TGF alpha-like properties secreted from the estrogen receptor negative breast cancer cell line MDA-MB-231. This factor was immunoprecipitated by an anti-TGF alpha polyclonal antibody and also had TGF alpha-like biological activity, as assayed b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00147a018
更新日期：1992-08-18 00:00:00
abstract：:Deposition of amyloid beta-peptide (Abeta) into amyloid plaques is one of the invariant neuropathological features of Alzheimer's disease. Proteins that codeposit with Abeta are potentially important for the pathogenesis, and a recently discovered plaque-associated protein is the collagenous Alzheimer amyloid plaque c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051263e
更新日期：2005-11-29 00:00:00
abstract：:For the study of the protein--protein and protein--nucleic acid interactions in the assembly of virus particles, laser Raman spectra have been obtained in H2O and D2O solutions and as a function of temperature for the following Salmonella phage P22 components: mature phage particles, isolated mature phage DNA, mature ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00259a023
更新日期：1982-08-03 00:00:00
abstract：:Cytochrome P450 3A4 is a drug-metabolizing enzyme of extraordinarily broad substrate specificity. This quality imparts upon the enzyme special importance in understanding its determinants of activity and substrate recognition. Limited successes in P450 3A4 active-site structure studies have been achieved by use of mec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049231z
更新日期：2005-02-15 00:00:00
abstract：:In this work, we obtain the data needed to predict chemical interactions of polyethylene glycols (PEGs) and glycerol with proteins and related organic compounds and thereby interpret or predict chemical effects of PEGs on protein processes. To accomplish this, we determine interactions of glycerol and tetraEG with >30...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00246
更新日期：2015-06-09 00:00:00
abstract：:The conformation of the glycerol group of a number of diacyl and monoacyl (lyso) phospholipids differing in the chemical nature of the head group was studied by 1H high-resolution NMR and X-ray crystallography. The NMR measurements were carried out with solutions or micellar dispersions of the lipids in deuteriated or...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00426a014
更新日期：1988-12-27 00:00:00
abstract：:In bacteria, regulatory phosphorylation of proteins at serine and/or threonine residues by Ser/Thr protein kinase (STPK) is an emerging theme in prokaryotic signaling, particularly since the prediction of the occurrence of several STPKs from genome sequencing and sequence surveys. Here we show that protein PknH posses...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035150b
更新日期：2003-12-30 00:00:00
abstract：:The three lipoyl (E2plip) domains of the dihydrolipoyl acetyltransferase component of the pyruvate dehydrogenase (PDH) complex of Escherichia coli house the lipoyl-lysine side chain essential for active-site coupling and substrate channelling within the complex. The structure of the unlipoylated form of the innermost ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992978i
更新日期：2000-07-25 00:00:00
abstract：:The effect of myosin on the structure of two sequences on G-actin, a loop between residues 39 and 52 and a segment between residues 61 and 69 from the NH2-terminus, was probed by limited proteolytic digestions of G-actin in the presence of the myosin subfragment 1 isozyme S-1(A2). Under the experimental conditions of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00126a014
更新日期：1992-03-24 00:00:00
