Structure and catalytic mechanism of N(5),N(10)-methenyl-tetrahydromethanopterin cyclohydrolase.

abstract：:31P NMR spectroscopy of butyrylcholinesterase (BChE), acetylcholinesterase (AChE), and chymotrypsin (Cht) inhibited by pinacolyl methylphosphonofluoridate (soman), methylphosphonodifluoridate (MPDF), and diisopropyl phosphorofluoridate (DFP) allowed direct observation of the OP-linked moiety of aged (nonreactivatable)...

journal_title：Biochemistry

authors： Segall Y,Waysbort D,Barak D,Ariel N,Doctor BP,Grunwald J,Ashani Y

更新日期：1993-12-14 00:00:00

abstract：:Lipocortin I is a 39-kilodalton membrane-associated protein that in A431 cells is phosphorylated on tyrosine in response to epidermal growth factor (EGF). We have used recombinant human lipocortin I as a substrate for several protein kinases and identified phosphorylated residues by a combination of peptide mapping an...

journal_title：Biochemistry

authors： Varticovski L,Chahwala SB,Whitman M,Cantley L,Schindler D,Chow EP,Sinclair LK,Pepinsky RB

更新日期：1988-05-17 00:00:00

abstract：:We review signaling by reactive oxygen species, which is emerging as a major physiological process. However, among the reactive oxygen species, H(2)O(2) best fulfills the requirements of being a second messenger. Its enzymatic production and degradation, along with the requirements for the oxidation of thiols by H(2)O...

journal_title：Biochemistry

authors： Forman HJ,Maiorino M,Ursini F

更新日期：2010-02-09 00:00:00

abstract：:Using the thrombin-cut [68-30 kilodalton (kDa)] myosin subfragment 1 (S-1) whose heavy chain has been selectively split within the central 50-kDa region, at Lys-560, with concomitant specific alterations of the ATPase and actin binding properties [Chaussepied, P., Mornet, D., Audemard, E., Derancourt, J., & Kassab, R....

journal_title：Biochemistry

authors： Chaussepied P,Mornet D,Kassab R

更新日期：1986-10-21 00:00:00

abstract：:Self-assembly of beta-sheet domains resulting in the formation of pathogenic, fibrillar protein aggregates (amyloids) is a characteristic feature of various medical disorders. These include neurodegenerative diseases, such as Alzheimer's, Huntington's, and Creutzfeldt-Jacob's. A significant problem in studying such ag...

journal_title：Biochemistry

authors： Janek K,Behlke J,Zipper J,Fabian H,Georgalis Y,Beyermann M,Bienert M,Krause E

更新日期：1999-06-29 00:00:00

abstract：:Intrinsic disorder is at the center of biochemical regulation and is particularly overrepresented among the often multifunctional viral proteins. Replication and transcription of the respiratory syncytial virus (RSV) relies on a RNA polymerase complex with a phosphoprotein cofactor P as the structural scaffold, which ...

journal_title：Biochemistry

authors： Noval MG,Esperante SA,Molina IG,Chemes LB,Prat-Gay Gd

更新日期：2016-03-15 00:00:00

abstract：:The interaction of the Escherichia coli single-stranded DNA binding protein (SSB) with oligo- and poly-nucleotides has been studied by 270-MHz 1H NMR spectroscopy and fast kinetic techniques. d(pT)8 and poly(dT) were used to study noncooperative and cooperative binding, respectively. The H6, H1', and CH3 resonances of...

journal_title：Biochemistry

authors： Römer R,Schomburg U,Krauss G,Maass G

更新日期：1984-12-04 00:00:00

abstract：:Intestinal fatty acid binding protein (IFABP) and liver FABP (LFABP), homologous proteins expressed at high levels in intestinal absorptive cells, employ markedly different mechanisms for the transfer of fatty acids (FAs) to acceptor membranes. Transfer from IFABP occurs during protein-membrane collisional interaction...

journal_title：Biochemistry

authors： Córsico B,Liou HL,Storch J

更新日期：2004-03-30 00:00:00

abstract：:We investigated the dependence of certain properties of bile salt/lecithin mixed micelles on the molar ratio of the two components. UV absorption suggested that the micellar system is fundamentally altered on passing through a bile salt:lecithin molar ratio of 1.8:1. Differential scanning calorimetry confirmed this tr...

journal_title：Biochemistry

authors： Claffey WJ,Holzbach RT

更新日期：1981-01-20 00:00:00

abstract：:Using cysteine mutagenesis and chemical modification by methanethiosulfonate derivatives, it was demonstrated that the external putative loop, joining transmembrane segments (TM's) IV-V of rabbit Na+/glucose cotransporter, rSGLT1, forms part of a Na+ binding and voltage sensing domain. Within this region, exposure to ...

journal_title：Biochemistry

authors： Huntley SA,Krofchick D,Silverman M

更新日期：2006-04-11 00:00:00

abstract：:An expression plasmid encoding the extracellular portion of the human tumor necrosis factor (TNF) type 1 receptor (TNF-R1) was constructed and used to generate a stable cell line secreting soluble TNF-R1 (sTNF-R1). The sTNF-R1 was purified, and its biochemical properties and its interactions with human TNF-alpha were ...

journal_title：Biochemistry

authors： Pennica D,Kohr WJ,Fendly BM,Shire SJ,Raab HE,Borchardt PE,Lewis M,Goeddel DV

更新日期：1992-02-04 00:00:00

abstract：:The photocycle of 124 kDa phytochrome A from Avena sativa was studied by Fourier-transform infrared spectroscopy at low temperatures. Difference spectra between the parent state Pr and the intermediates of the Pr-->Pfr pathway, i.e. lumi-R, meta-Ra, and meta-Rc, and between Pfr and the intermediates of the Pfr-->Pr pa...

journal_title：Biochemistry

authors： Foerstendorf H,Mummert E,Schäfer E,Scheer H,Siebert F

更新日期：1996-08-20 00:00:00

abstract：:Azurin from Pseudomonas aeruginosa and two mutants where the methionine ligand has been mutated have been studied in order to directly investigate the functional and structural significance of this ligand in the blue copper proteins. Reduction potentials, X-ray absorption fine structure (XAFS), electron paramagnetic r...

journal_title：Biochemistry

authors： Murphy LM,Strange RW,Karlsson BG,Lundberg LG,Pascher T,Reinhammar B,Hasnain SS

更新日期：1993-03-02 00:00:00

abstract：:This report presents evidence for interdomain steps of the ligand-coupled signal transduction mechanism of the oxygen receptor from Rhizobium meliloti, RmFixL. Photolysis of the CO adducts of heme domain (RmFixLN) and heme kinase (RmFixL*) proteins allowed tracking of second-order heme CO recombination reactions by tr...

journal_title：Biochemistry

authors： Rodgers KR,Tang L,Lukat-Rodgers GS,Wengenack NL

更新日期：2001-10-30 00:00:00

abstract：:The constant (CL) fragment of the immunoglobulin light chain contains only one intrachain disulfide bond buried in the interior of the molecule. The kinetics of reduction with dithiothreitol of the disulfide bond were studied at various concentrations of guanidine hydrochloride at pH 8.0 and 25 degrees C. It was found...

journal_title：Biochemistry

authors： Kikuchi H,Goto Y,Hamaguchi K

更新日期：1986-04-22 00:00:00

abstract：:Resonance Raman spectroscopy has been used to study milk xanthine oxidase, an enzyme containing molybdenum, binuclear iron-sulfur clusters, and FAD as cofactors. The contribution of FAD dominates the resonance Raman spectrum at frequencies above 500 cm-1. As expected, no bands assignable to FAD are observed in deflavo...

journal_title：Biochemistry

authors： Willis LJ,Loehr TM

更新日期：1985-05-21 00:00:00

abstract：:The flavocytochrome p-cresol methylhydroxylase from Pseudomonas putida has been reported to have a Mr of 114,000 and to consist of two subunits, a flavoprotein and a cytochrome c, each with a Mr of 58,000. Recent X-ray crystallographic data from our laboratories [Shamala, N., Lim, L. W., Mathews, F. S., McIntire, W., ...

journal_title：Biochemistry

authors： McIntire W,Singer TP,Smith AJ,Mathews FS

更新日期：1986-10-07 00:00:00

abstract：:High-affinity mu-conotoxin block of skeletal muscle Na+ channels depends on an arginine at position 13 (Arg-13). To understand both the mechanism of toxin interaction and the general structure of its binding site in the channel mouth, we examined by thermodynamic mutant cycle analysis the interaction between the criti...

journal_title：Biochemistry

authors： Chang NS,French RJ,Lipkind GM,Fozzard HA,Dudley S Jr

更新日期：1998-03-31 00:00:00

abstract：:The D-alanyl-D-alanine-adding enzyme encoded by the murF gene catalyzes the ATP-dependent formation of UDP-N-acetylmuramyl-L-gamma-D-Glu-meso-diaminopimelyl-D-Ala-D-Ala (UDP-MurNAc-tripeptide). MurF has been cloned from Escherichia coli and expressed as a glutathione S-transferase (GST) fusion using the tac promoter-b...

journal_title：Biochemistry

authors： Anderson MS,Eveland SS,Onishi HR,Pompliano DL

更新日期：1996-12-17 00:00:00

abstract：:We studied the binding of peptides containing five basic residues to membranes containing acidic lipids. The peptides have five arginine or lysine residues and zero, one, or two alanines between the basic groups. The vesicles were formed from mixtures of a zwitterionic lipid, phosphatidylcholine, and an acidic lipid, ...

journal_title：Biochemistry

authors： Mosior M,McLaughlin S

更新日期：1992-02-18 00:00:00

abstract：:The bacterial reaction center (RC) converts light into chemical energy through the reduction of an internal quinone molecule Q(B) to Q(B)H(2). In the native RC, proton transfer is coupled to electron transfer and is not rate-controlling. Consequently, proton transfer is not directly observable, and its rate was unknow...

journal_title：Biochemistry

authors： Paddock ML,Adelroth P,Feher G,Okamura MY,Beatty JT

更新日期：2002-12-17 00:00:00

abstract：:At neutral pH, compound I of Arthromyces ramosus peroxidase (ARP) was stable and was reduced to ferric ARP without apparent formation of compound II upon titration with ascorbate or hydroquinone. In the titration experiments, compound II was seen as an intermediate only at alkaline pH. However, measuring a difference ...

journal_title：Biochemistry

authors： Farhangrazi ZS,Copeland BR,Nakayama T,Amachi T,Yamazaki I,Powers LS

更新日期：1994-05-10 00:00:00

abstract：:TCDD (dioxin) induces a rapid inflammatory response from 3T3-L1 adipocytes as judged by prominent induction of the mRNA expression of prostaglandin-endperoxide synthase 2 (Cox-2) along with other inflammation markers within 1 h. This action of TCDD is clearly antagonized by cell pretreatment with AACOCF3 (an inhibitor...

journal_title：Biochemistry

authors： Li W,Matsumura F

更新日期：2008-12-30 00:00:00

abstract：:Monte Carlo protein simulations with continuum solvation were used to explore the conformational mobility of NO within the active site of metmyoglobin. To the best of our knowledge this is the first application of a continuum solvation model for exploring protein binding sites. The usefulness of the Monte Carlo confor...

journal_title：Biochemistry

authors： Keserü GM,Menyhárd DK

更新日期：1999-05-18 00:00:00

abstract：:The structural domains of human apolipoprotein(a) [apo(a)] and its interaction with apolipoprotein B-100 (apo B-100) in the lipoprotein(a) [Lp(a)] particle were investigated by limited proteolysis with thermolysin and cathepsin D. We characterized the proteolytic products by sodium dodecyl sulfate-polyacrylamide gradi...

journal_title：Biochemistry

authors： Huby T,Doucet C,Dieplinger H,Chapman J,Thillet J

更新日期：1994-03-22 00:00:00

abstract：:The solution secondary structure of the highly active Y55F/Y88F "Tyr-14-only" mutant of delta 5-3-ketosteroid isomerase complexed with 19-nortestosterone hemisuccinate has been shown to consist of three helices, a six-stranded mixed beta-sheet, and five turns. The steroid binds near the general acid, Tyr-14, on helix ...

journal_title：Biochemistry

authors： Zhao Q,Abeygunawardana C,Gittis AG,Mildvan AS

更新日期：1997-12-02 00:00:00

abstract：:X-ray absorption spectroscopy at the Zn K-edge indicates that the active site of the marine diatom Thalassiosira weissflogii carbonic anhydrase is strikingly similar to that of mammalian alpha-carbonic anhydrase enzymes. The zinc has three histidine ligands and a single water at 1.98 A. This is quite different from th...

journal_title：Biochemistry

authors： Cox EH,McLendon GL,Morel FM,Lane TW,Prince RC,Pickering IJ,George GN

更新日期：2000-10-10 00:00:00

abstract：:The folding of the enzyme indole-3-glycerol-phosphate synthase (IGPS), a member of the (alpha/beta)8 fold family, has been studied. At least two folding intermediates have been detected using spectroscopic and activity measurements in combination with gel filtration chromatography. These two intermediates are produced...

journal_title：Biochemistry

authors： Sánchez del Pino MM,Fersht AR

更新日期：1997-05-06 00:00:00

abstract：:The FAD-dependent alpha-glycerophosphate oxidase (GlpO) from Enterococcus casseliflavus and Streptococcus sp. was originally studied as a soluble flavoprotein oxidase; surprisingly, the GlpO sequence is 30-43% identical to those of the alpha-glycerophosphate dehydrogenases (GlpDs) from mitochondrial and bacterial sour...

journal_title：Biochemistry

authors： Colussi T,Parsonage D,Boles W,Matsuoka T,Mallett TC,Karplus PA,Claiborne A

更新日期：2008-01-22 00:00:00

abstract：:We show here that expression of chromogranins in non-neuroendocrine NIH3T3 cells significantly increased the amount of IP(3)-mediated intracellular Ca(2+) mobilization in these cells, whereas suppression of them in neuroendocrine PC12 cells decreased the amount of mobilized Ca(2+). We have therefore investigated the r...

journal_title：Biochemistry

authors： Huh YH,Jeon SH,Yoo JA,Park SY,Yoo SH

更新日期：2005-04-26 00:00:00