The active site structure of Thalassiosira weissflogii carbonic anhydrase 1.

abstract：:Genomic clones containing the human Zn-alpha 2-glycoprotein gene were isolated. Two of them were for the functional gene overlapped, and the other four were for two different pseudogenes (1 and 2) retaining exon-intron organization. The complete DNA sequence of the functional gene (9.3 kb) and its 5'- and 3'-flanking ...

journal_title：Biochemistry

authors： Ueyama H,Deng HX,Ohkubo I

更新日期：1993-12-07 00:00:00

abstract：:Hormone-sensitive lipase (HSL) is a rate-limiting enzyme in lipolysis that displays broad substrate specificity. HSL function is regulated by reversible phosphorylation that occurs within a 150 aa "regulatory module" of the protein. The current studies used mutational analysis to dissect the contribution of the "regul...

journal_title：Biochemistry

authors： Wang J,Shen WJ,Patel S,Harada K,Kraemer FB

更新日期：2005-02-15 00:00:00

abstract：:A 31 amino acid fragment of the extracellular N-terminus of the human G-protein coupled receptor for parathyroid hormone (PTH1R) has been structurally characterized by NMR and molecular dynamics simulations. The fragment PTH1R[168-198] includes residues 173-189, shown by photoaffinity cross-linking to be a contact dom...

journal_title：Biochemistry

authors： Pellegrini M,Bisello A,Rosenblatt M,Chorev M,Mierke DF

更新日期：1998-09-15 00:00:00

abstract：:Site-directed mutagenesis of the aspartate receptor of Salmonella typhimurium (Tars) at serine 68, a residue located within the aspartate binding pocket and at the subunit interface, identified this residue as an allosteric switch in this receptor. Substitutions at this position can affect both the type and degree of ...

journal_title：Biochemistry

authors： Kolodziej AF,Tan T,Koshland DE Jr

更新日期：1996-11-26 00:00:00

abstract：:Retinal membrane guanylyl cyclase (RetGC) in the outer segments of vertebrate photoreceptors is controlled by guanylyl cyclase activating proteins (GCAPs), responding to light-dependent changes of the intracellular Ca(2+) concentrations. We present evidence that a different RetGC binding protein, retinal degeneration ...

journal_title：Biochemistry

authors： Peshenko IV,Olshevskaya EV,Azadi S,Molday LL,Molday RS,Dizhoor AM

更新日期：2011-11-08 00:00:00

abstract：:Iron is vital for almost all organisms because of its ability to donate and accept electrons with relative ease. It serves as a cofactor for many proteins and enzymes necessary for oxygen and energy metabolism, as well as for several other essential processes. Mammalian cells utilize multiple mechanisms to acquire iro...

journal_title：Biochemistry

authors： Pantopoulos K,Porwal SK,Tartakoff A,Devireddy L

更新日期：2012-07-24 00:00:00

abstract：:Substrates homoprotocatechuate (HPCA) and O(2) bind to the Fe(II) of homoprotocatechuate 2,3-dioxygenase (FeHPCD) in adjacent coordination sites. Transfer of an electron(s) from HPCA to O(2) via the iron is proposed to activate the substrates for reaction with each other to initiate aromatic ring cleavage. Here, rapid...

journal_title：Biochemistry

authors： Mbughuni MM,Chakrabarti M,Hayden JA,Meier KK,Dalluge JJ,Hendrich MP,Münck E,Lipscomb JD

更新日期：2011-11-29 00:00:00

abstract：:NG-Methyl-L-arginine (L-NMA) is one of the most commonly used inhibitors of the nitric oxide synthases (NOS). Results reported here demonstrate that L-NMA is an alternate substrate and a mechanism-based inhibitor of the inducible NOS purified from murine macrophages. The irreversible inhibition displays pseudo-first-o...

journal_title：Biochemistry

authors： Olken NM,Marletta MA

更新日期：1993-09-21 00:00:00

abstract：:Two extremely potent inhibitors of citrate synthase, carboxyl and primary amide analogues of acetyl coenzyme A, have been synthesized. The ternary complexes of these inhibitors with oxaloacetate and citrate synthase have been crystallized and their structures analyzed at 1.70- and 1.65-A resolution, respectively. The ...

journal_title：Biochemistry

authors： Usher KC,Remington SJ,Martin DP,Drueckhammer DG

更新日期：1994-06-28 00:00:00

abstract：:Electron spin resonance (ESR) spectroscopy using spin-labeled ATP was used to study nucleotide binding to and structural transitions within the multidrug resistance P-glycoprotein, P-gp. Spin-labeled ATP (SL-ATP) with the spin label attached to the ribose, was observed to be an excellent substrate analogue for P-gp. S...

journal_title：Biochemistry

authors： Delannoy S,Urbatsch IL,Tombline G,Senior AE,Vogel PD

更新日期：2005-10-25 00:00:00

abstract：:The sigmoidal dependence of activity on substrate concentration exhibited by the regulatory enzyme aspartate transcarbamoylase (ATCase) of Escherichia coli is generally attributed to a ligand-promoted change in the quaternary structure of the enzyme. Although a global conformational change in ATCase upon the binding o...

journal_title：Biochemistry

authors： Eisenstein E,Markby DW,Schachman HK

更新日期：1990-04-17 00:00:00

abstract：:Mycobacterium smegmatis MshC catalyzes the ATP-dependent condensation of GlcN-Ins and l-cysteine to form l-Cys-GlcN-Ins, the penultimate step in mycothiol biosynthesis. Attempts to crystallize the native, full-length MshC have been unsuccessful. However, incubation of the enzyme with the cysteinyl adenylate analogue, ...

journal_title：Biochemistry

authors： Tremblay LW,Fan F,Vetting MW,Blanchard JS

更新日期：2008-12-16 00:00:00

abstract：:The interaction of the monofunctional chorismate mutase from Bacillus subtilis with chorismate and prephenate has been studied kinetically and by NMR spectroscopy with 13C specifically labeled substrates. Prephenate dominates the population of enzyme-bound species, and the "off" rate constant (approximately 60 s-1) ob...

journal_title：Biochemistry

authors： Gray JV,Eren D,Knowles JR

更新日期：1990-09-18 00:00:00

abstract：:We investigated the molecular mechanisms and the binding site location for the fluorophor crystal violet (CrV), a noncompetitive antagonist of the nicotinic acetylcholine receptor (AChR). To this end, radiolabeled competition binding, fluorescence spectroscopy, Schild-type analysis, patch-clamp recordings, and molecul...

journal_title：Biochemistry

authors： Arias HR,Bhumireddy P,Spitzmaul G,Trudell JR,Bouzat C

更新日期：2006-02-21 00:00:00

abstract：:Ryanodine receptor (RyR) mutations linked with some congenital skeletal and cardiac diseases are localized to three easily definable regions: region 1 (N-terminal domain), region 2 (central domain), and a rather broad region 3 containing the channel pore. As shown in our recent studies, the interdomain interaction bet...

journal_title：Biochemistry

authors： Hamada T,Bannister ML,Ikemoto N

更新日期：2007-04-10 00:00:00

abstract：:RNAs are involved in myriad cellular events. In general, RNA function is affected by cellular conditions. For instance, molecular crowding promotes RNA folding through compaction of the RNA. Metabolites generally destabilize RNA secondary structure, which improves RNA folding cooperativity and increases the fraction o...

journal_title：Biochemistry

authors： Yamagami R,Huang R,Bevilacqua PC

更新日期：2019-09-24 00:00:00

abstract：:Fluorescence lifetimes and fluorescence anisotropy decays of the voltage-sensitive styryl-pyridinium dye RH421 have been measured in the presence of dimyristoylphosphatidylcholine vesicles, the water soluble enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco), and Na+,K(+)-ATPase-containing membrane fragm...

journal_title：Biochemistry

authors： Visser NV,van Hoek A,Visser AJ,Frank J,Apell HJ,Clarke RJ

更新日期：1995-09-19 00:00:00

abstract：:Fibronectin (FN) is an extracellular matrix (ECM) protein found soluble in corporal fluids or as an insoluble fibrillar component incorporated in the ECM. This phenomenon implicates structural changes that expose FN binding sites and activate the protein to promote intermolecular interactions with other FN. We have in...

journal_title：Biochemistry

authors： Patel S,Chaffotte AF,Goubard F,Pauthe E

更新日期：2004-02-17 00:00:00

abstract：:[15N]Glycine was biosynthetically incorporated into normal cellular N-ras p21 and a position 12 transforming mutant, in order to produce p21 proteins containing several site-specific NMR probes at or near activating positions in the guanine nucleotide binding domain. We have previously assigned all five glycine resona...

journal_title：Biochemistry

authors： Campbell-Burk S

更新日期：1989-11-28 00:00:00

abstract：:Phosphoinositide 3-kinase gamma (PI3Kgamma) is activated by Gbetagamma release after stimulation of Galpha i -coupled receptors, involving a recruitment of the enzyme to the plasma membrane via interaction of the regulatory subunit p101 or p87 with Gbetagamma. The receptor-mediated release of Gbetagamma was, however, ...

journal_title：Biochemistry

authors： Tannert A,Voigt P,Burgold S,Tannert S,Schaefer M

更新日期：2008-10-28 00:00:00

abstract：:Synaptotagmin 1 (syt1) is a synaptic vesicle-anchored membrane protein that acts as the calcium sensor for the synchronous component of neuronal exocytosis. Using site-directed spin labeling, the position and membrane interactions of a fragment of syt1 containing its two C2 domains (syt1C2AB) were assessed in bilayers...

journal_title：Biochemistry

authors： Kuo W,Herrick DZ,Cafiso DS

更新日期：2011-04-05 00:00:00

abstract：:We report on the determination of the solution structure of two sequence-related oligonucleotides, d(GTACGTAC)2 and d(CATCGATG)2. Results have been obtained by using a combined approach of (a) two-dimensional NMR, including proton and phosphorus experiments, (b) restrained molecular mechanics performed with sugar phas...

journal_title：Biochemistry

authors： Lefebvre A,Mauffret O,Hartmann B,Lescot E,Fermandjian S

更新日期：1995-09-19 00:00:00

abstract：:The C2A domain of synaptotagmin 7 (Syt7) is a Ca(2+) and membrane binding module that docks and inserts into cellular membranes in response to elevated intracellular Ca(2+) concentrations. Like other C2 domains, Syt7 C2A binds Ca(2+) and membranes primarily through three loop regions; however, it docks at Ca(2+) conce...

journal_title：Biochemistry

authors： Chon NL,Osterberg JR,Henderson J,Khan HM,Reuter N,Knight JD,Lin H

更新日期：2015-09-22 00:00:00

abstract：:The proton-translocating NADH:ubiquinone oxidoreductase of respiratory chains (complex I) contains one flavin mononucleotide and five EPR-detectable iron-sulfur clusters as redox groups. Because of the number of conserved motifs typical for binding iron-sulfur clusters and the high content of iron and acid-labile sulf...

journal_title：Biochemistry

authors： Rasmussen T,Scheide D,Brors B,Kintscher L,Weiss H,Friedrich T

更新日期：2001-05-22 00:00:00

abstract：:Recent studies have indicated that CYP3A4 exhibits non-Michaelis-Menten kinetics for numerous substrates. Both homo- and heterotropic activation have been reported, and kinetic models have suggested multiple substrates within the active site. We provide some of the first physicochemical data supporting the hypothesis ...

journal_title：Biochemistry

authors： Cameron MD,Wen B,Allen KE,Roberts AG,Schuman JT,Campbell AP,Kunze KL,Nelson SD

更新日期：2005-11-01 00:00:00

abstract：:31P NMR spectra of equilibrium mixtures of enzyme-bound reactants and products of the adenylate kinase reaction (formula; see text) were analyzed by using computer simulations based on density matrix theory of chemical exchange. Since adenylate kinase has the unique feature that the reactants in the reverse direction ...

journal_title：Biochemistry

authors： Vasavada KV,Kaplan JI,Nageswara Rao BD

更新日期：1984-02-28 00:00:00

abstract：:The proline catabolic enzyme Δ(1)-pyrroline-5-carboxylate dehydrogenase (ALDH4A1) catalyzes the NAD(+)-dependent oxidation of γ-glutamate semialdehyde to l-glutamate. In Saccharomyces cerevisiae, ALDH4A1 is encoded by the PUT2 gene and known as Put2p. Here we report the steady-state kinetic parameters of the purified ...

journal_title：Biochemistry

authors： Pemberton TA,Srivastava D,Sanyal N,Henzl MT,Becker DF,Tanner JJ

更新日期：2014-03-04 00:00:00

abstract：:The protease activity of hepatitis C virus nonstructural protein 3 (NS3) is essential for viral replication. ITMN-191, a macrocyclic inhibitor of the NS3 protease active site, promotes rapid, multilog viral load reductions in chronic HCV patients. Here, ITMN-191 is shown to be a potent inhibitor of NS3 with a two-step...

journal_title：Biochemistry

authors： Rajagopalan R,Misialek S,Stevens SK,Myszka DG,Brandhuber BJ,Ballard JA,Andrews SW,Seiwert SD,Kossen K

更新日期：2009-03-24 00:00:00

abstract：:We have studied alkylation of the acetylcholine receptor by N-[3H]ethylmaleimide ([3H]NEM) under various conditions. The radiolabeled preparations were submitted to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to separate the receptor complex into subunits, and the incorporation of 3H into each type of ch...

journal_title：Biochemistry

authors： de Souza Otero A,Hamilton SL

更新日期：1984-05-22 00:00:00

abstract：:It had been previously reported that aromatic amines were not substrates for the bacterial quinoprotein methylamine dehydrogenase. In this study, benzylamine-dependent activity was also not observed in the steady-state assay of this enzyme with the artificial electron acceptor phenazine ethosulfate (PES). Benzylamines...

journal_title：Biochemistry

authors： Davidson VL,Jones LH,Graichen ME

更新日期：1992-04-07 00:00:00