Monofunctional chorismate mutase from Bacillus subtilis: kinetic and 13C NMR studies on the interactions of the enzyme with its ligands.

abstract：:Oligothymidylates covalently linked to iron-porphyrins were synthesized to target the nuclease activity of Fe-porphyrin to complementary polynucleotides. In the presence of oxygen and a reducing agent, oligo(dT)7 bearing the reactive group attached to the 3'-phosphate was shown to be active in the cleavage of poly(dA)...

journal_title：Biochemistry

authors： Le Doan T,Perrouault L,Helene C,Chassignol M,Thuong NT

更新日期：1986-11-04 00:00:00

abstract：:The crystal structures of the deoxy and carbonmonoxy forms of a distal pocket myoglobin mutant in which valine68(E11) is replaced by threonine have been solved to 2.1- and 2.2-A resolution, respectively. This substitution has been shown previously to cause large decreases in the rate of oxygen binding and to lower the...

journal_title：Biochemistry

authors： Cameron AD,Smerdon SJ,Wilkinson AJ,Habash J,Helliwell JR,Li T,Olson JS

更新日期：1993-12-07 00:00:00

abstract：:Arginine-binding RNA motifs are important to protein-RNA interaction and perhaps also for Archean biochemistry. Selection-amplification was used to isolate three RNAs that are eluted by free arginine from an L-arginine affinity column (Kd approximately 0.2-0.4 mM). The binding sites contain specific internal and bulge...

journal_title：Biochemistry

authors： Connell GJ,Illangesekare M,Yarus M

更新日期：1993-06-01 00:00:00

abstract：:We have previously shown that the anticodon of methionine tRNAs contains most, if not all, of the nucleotides required for specific recognition of tRNA substrates by Escherichia coli methionyl-tRNA synthetase [Schulman, L. H., & Pelka, H. (1988) Science 242, 765-768]. Previous cross-linking experiments have also ident...

journal_title：Biochemistry

authors： Ghosh G,Pelka H,Schulman LH

更新日期：1990-03-06 00:00:00

abstract：:By suspending bovine rhodopsin in trehalose-water glass films, it is possible to trap photostates in the light-activation process. Because of the unusually high vitrification temperature of trehalose-water mixtures, this trapping can be accomplished at room temperature. This allows for a facile investigation of the sp...

journal_title：Biochemistry

authors： Sikora S,Little AS,Dewey TG

更新日期：1994-04-19 00:00:00

abstract：:Over 70 transthyretin (TTR) mutations facilitate amyloidosis in tissues other than the central nervous system (CNS). In contrast, the D18G TTR mutation in individuals of Hungarian descent leads to CNS amyloidosis. D18G forms inclusion bodies in Escherichia coli, unlike the other disease-associated TTR variants overexp...

journal_title：Biochemistry

authors： Hammarström P,Sekijima Y,White JT,Wiseman RL,Lim A,Costello CE,Altland K,Garzuly F,Budka H,Kelly JW

更新日期：2003-06-10 00:00:00

abstract：:Histone deacetylase 8 (HDAC8) is a well-characterized member of the class I acetyl-lysine deacetylase (HDAC) family. Previous work has shown that the efficiency of HDAC8-catalyzed deacetylation of a methylcoumarin peptide varies depending on the identity of the divalent metal ion in the HDAC8 active site. Here we demo...

journal_title：Biochemistry

authors： Castaneda CA,Lopez JE,Joseph CG,Scholle MD,Mrksich M,Fierke CA

更新日期：2017-10-24 00:00:00

abstract：:Hepatic microsomal cytochrome P-450 from phenobarbital-pretreated rats is destroyed by propyne in a reduced nicotinamide adenine dinucleotide dependent process which also results in vivo in the accumulation of an abnormal green porphyrin. The green porphyrin has been identified by its electronic absorption, mass spect...

journal_title：Biochemistry

authors： Ortiz de Montellano PR,Kunze KL

更新日期：1981-12-08 00:00:00

abstract：:Both activities of the aspartokinase--homoserine I (AK-HSD) of Escherichia coli are inhibited by threonine. Careful threonine binding studies have now been done which have allowed us to distinguish the various effects of threonine on the enzyme. The ultrafiltration technique of H. Paulus ((1969) Anal. Biochem. 32, 101...

journal_title：Biochemistry

authors： Bearer CF,Neet KE

更新日期：1978-08-22 00:00:00

abstract：:Opossum (Didelphis virginiana) serum was fractionated with (NH4)2SO4 and then chromatographed on DEAE-Sepharose and phenyl-Sepharose. Affinity chromatography on a protein A-Sepharose-antibody column removed traces of opossum serum metalloproteinase inhibitors, and resulted in a homogeneous preparation of opossum alpha...

journal_title：Biochemistry

authors： Catanese JJ,Kress LF

更新日期：1993-01-19 00:00:00

abstract：:Previous work has indicated that following the rapid adenosine 5'-triphosphate (ATP) induced dissociation of the microtubule-dynein complex, the rate-limiting step in the ATPase cycle is product release [Johnson, K. A. (1983) J. Biol. Chem. 258, 13825-13832], which occurs at a rate of approximately 2-6 s-1. In this re...

journal_title：Biochemistry

authors： Omoto CK,Johnson KA

更新日期：1986-01-28 00:00:00

abstract：:Reporter gene transactivation by human p53 is inhibited in budding yeast lacking the TRR1 gene encoding thioredoxin reductase. To investigate the role of thioredoxin in controlling p53 activity, the level of reporter gene transactivation by p53 was determined in yeast lacking the TRX1 and TRX2 genes encoding cytosolic...

journal_title：Biochemistry

authors： Stoner CS,Pearson GD,Koç A,Merwin JR,Lopez NI,Merrill GF

更新日期：2009-09-29 00:00:00

abstract：:Polyclonal antibody to mitochondrial P-450c27/25 reacted with two proteins of apparent molecular masses of 52 kilodaltons (kDa) and 50 kDa from the female rat liver mitochondrial proteins bound to an omega-octylaminoagarose column. The two proteins were purified to greater than 85% homogeneity by DEAE-Sephacel and hyd...

journal_title：Biochemistry

authors： Addya S,Zheng YM,Shayiq RM,Fan JY,Avadhani NG

更新日期：1991-08-27 00:00:00

abstract：:A new procedure for the synthesis of the pyrophosphate bond has been employed in the preparation of nucleoside dipyrophosphates from nucleoside 3',5'-diphosphates. The method makes use of a powerful phosphorylating agent generated in a mixture of cyanoethyl phosphate, dicyclohexylcarbodiimide, and mesitylenesulfonyl c...

journal_title：Biochemistry

authors： Bennett GN,Gough GR,Gilham PT

更新日期：1976-10-19 00:00:00

abstract：:Dihydroorotate dehydrogenases (DHODs) oxidize dihydroorotate (DHO) to orotate using the FMN prosthetic group to abstract a hydride equivalent from C6 and a protein residue (Ser for Class 2 DHODs) to deprotonate C5. The fundamental question of whether the scission of the two DHO C-H bonds is concerted or stepwise was a...

journal_title：Biochemistry

authors： Fagan RL,Nelson MN,Pagano PM,Palfey BA

更新日期：2006-12-19 00:00:00

abstract：:In recent years, there has been dramatic growth in the study of RNA. RNA has gone from being known as an intermediate in the central dogma of molecular biology to a molecule with a large diversity of structure and function that is involved in all aspects of biology. As new functions are rapidly discovered, it has beco...

journal_title：Biochemistry

authors： Moon MH,Hilimire TA,Sanders AM,Schneekloth JS Jr

更新日期：2018-08-07 00:00:00

abstract：:Although the addition of various divalent metals to beta-galactosidase resulted in apparent activation, only Mg2+ and Mn2+ actually did activate. The apparent activation by the other divalent metals was shown to be due to Mg2+ impurities. Calcium did not activate, but experiments suggested that it did bind. Other diva...

journal_title：Biochemistry

authors： Huber RE,Parfett C,Woulfe-Flanagan H,Thompson DJ

更新日期：1979-09-18 00:00:00

abstract：:To investigate cytosine protonation and its influence on the sequence-dependent thermal stability of DNA triplexes in detail, we have employed homo- and heteronuclear NMR experiments on specifically (15)N-labeled oligodeoxynucleotides that were designed to fold into intramolecular triple helices of the pyrimidine moti...

journal_title：Biochemistry

authors： Leitner D,Schröder W,Weisz K

更新日期：2000-05-16 00:00:00

abstract：:Stabilization of the plasma membrane Ca-ATPase (PMCA) in an inactive conformation upon oxidation of multiple methionines in the calcium regulatory protein calmodulin (CaM) is part of an adaptive cellular response to minimize ATP utilization and the generation of reactive oxygen species (ROS) under conditions of oxidat...

journal_title：Biochemistry

authors： Chen B,Mayer MU,Squier TC

更新日期：2005-03-29 00:00:00

abstract：:Filamin A (FLNA) is a ubiquitously expressed actin cross-linking protein and a scaffold of numerous binding partners to regulate cell proliferation, migration, and survival. FLNA is a homodimer, and each subunit has an N-terminal actin-binding domain followed by 24 immunoglobulin-like repeats (R). FLNA mediates mechan...

journal_title：Biochemistry

authors： Wang L,Nakamura F

更新日期：2019-11-26 00:00:00

abstract：:Evidence for the NADH-modulated formation of a complex between alpha-glycerol-3-phosphate dehydrogenase and l-lactate dehydrogenase was reported [Yong, H., Thomas, G. A., and Peticolas, W. L. (1993) Biochemistry 32, 11124-11131]. This NADH-modulated association suggested a mechanism of potentially great importance to ...

journal_title：Biochemistry

authors： Lehoux EA,Baker SM,Kovina MV,Hays FA,Spivey HO

更新日期：2003-05-27 00:00:00

abstract：:The transcriptional activity of the hexafluorinated derivative of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], 26,26,26,27,27,27-hexafluoro-1,25-dihydroxyvitamin D3 [F6-1,25-(OH)2D3], was examined in cultured cells by a transient expression assay (CAT assay) using expression vectors for the rat nuclear vitamin D3 receptor...

journal_title：Biochemistry

authors： Sasaki H,Harada H,Handa Y,Morino H,Suzawa M,Shimpo E,Katsumata T,Masuhiro Y,Matsuda K,Ebihara K

更新日期：1995-01-10 00:00:00

abstract：:Aquaporin-4 (AQP4) is the predominant water channel in the brain. Although AQP4 plays an important role in brain water homeostasis, the molecular mechanisms of AQP4 regulation are not fully understood. In this report, we show how Zn(2+) rapidly and reversibly decreases the water permeability of AQP4 when it is reconst...

journal_title：Biochemistry

authors： Yukutake Y,Hirano Y,Suematsu M,Yasui M

更新日期：2009-12-29 00:00:00

abstract：:Several snake venoms contain a phospholipase A2 in which position 49 in the active site is occupied by a lysine or a serine instead of the aspartate residue normally found. Although these proteins do not bind Ca2+ and are devoid of catalytic activity, they are still highly specific myotoxins and have recently been sho...

journal_title：Biochemistry

authors： Pedersen JZ,Lomonte B,Massoud R,Gubensek F,Gutiérrez JM,Rufini S

更新日期：1995-04-11 00:00:00

abstract：:6-[(4-Bromo-2,3-dioxobutyl)thio]-6-deaminoadenosine 5'-diphosphate (6-BDB-TADP) has been shown to react at the reduced diphosphopyridine nucleotide (DPNH) inhibitory site of bovine liver glutamate dehydrogenase with incorporation of 1 mol of reagent/mol of enzyme subunit [Batra, S. P., & Colman, R. F. (1984) Biochemis...

journal_title：Biochemistry

authors： Batra SP,Colman RF

更新日期：1986-06-17 00:00:00

abstract：:Protein L31 from the 50S ribosomal subunit of Escherichia coli was manually sequenced by the dansyl-Edman method. Owing to the availability of only small quantities of purified L31, sequencing methods were scaled down such that the entire primary structure could be determined with 700 microgram of protein. The techniq...

journal_title：Biochemistry

authors： Brosius J

更新日期：1978-02-07 00:00:00

abstract：:The fatty acid synthase (FAS) of animal tissue is a dimer of two identical subunits, each with a Mr of 260,000. The subunit is a single multifunctional protein having seven catalytic activities and a site for binding of the prosthetic group 4'-phosphopantetheine. The mRNA coding for the subunit has an estimated size o...

journal_title：Biochemistry

authors： Kasturi R,Chirala S,Pazirandeh M,Wakil SJ

更新日期：1988-10-04 00:00:00

abstract：:The expression of apoE mRNA in the adrenal gland is inversely correlated to steroidogenesis and directly correlated to the level of cholesteryl ester stores. To further investigate the relationship between apoE and cellular cholesterol homeostasis, several parameters of cholesterol metabolism in the murine Y1 adrenal ...

journal_title：Biochemistry

authors： Prack MM,Rothblat GH,Erickson SK,Reyland ME,Williams DL

更新日期：1994-05-03 00:00:00

abstract：:We have investigated the effect of increasing percentages of methylated pyrimidines on the structure of poly(dA-dU).poly(dA-dU). This was done by synthesizing analogous polynucleotides that contained deoxythymidine residues as well as deoxyuridine residues and observing their 31P NMR spectra in increasing amounts of C...

journal_title：Biochemistry

authors： Wu HY,Behe MJ

更新日期：1985-09-24 00:00:00

abstract：:EPR studies have revealed that removal of calcium using citric acid from the site in spinach photosystem II which is coupled to the photosynthetic O2-evolving process produces a structural change in the manganese cluster responsible for water oxidation. If done in the dark, this yields a modified S1' oxidation state w...

journal_title：Biochemistry

authors： Sivaraja M,Tso J,Dismukes GC

更新日期：1989-11-28 00:00:00