Abstract:
:The expression of apoE mRNA in the adrenal gland is inversely correlated to steroidogenesis and directly correlated to the level of cholesteryl ester stores. To further investigate the relationship between apoE and cellular cholesterol homeostasis, several parameters of cholesterol metabolism in the murine Y1 adrenal cell line engineered to constitutively express human apoE (Y1-E cells) have been studied. It is reported here that Y1-E cells have increased cellular cholesterol content and markedly reduced efflux of free cholesterol as compared to control Y1 cells that do not express apoE. Y1-E cells have increases in both free and esterified cholesterol. However, Y1 and Y1-E cells incorporate [14C]oleate into cholesteryl ester at similar rates and have similar levels of maximal ACAT activity in isolated microsomes. Turnover of cholesteryl ester stores prelabeled with [14C]oleate occurred at similar rates in Y1-E and control Y1 cells, suggesting that increased cholesteryl ester stores in Y1-E cells do not result from reduced cholesteryl ester hydrolysis. Y1-E cells showed reduced cholesterol efflux as compared to control Y1 cells with either native high-density lipoprotein or cholesterol-free reconstituted particles as extracellular acceptors. Cholesterol efflux was not altered by inhibition of ACAT, suggesting that cholesterol esterification in Y1-E cells is not inhibiting efflux. These results suggest that reduced cholesterol efflux is responsible, at least in part, for the cholesterol accumulation in Y1-E cells. In comparison to the rat adrenal gland in vivo, Y1-E cells resemble adrenocortical cells under conditions where steroidogenesis is suppressed and apoE expression and cholesteryl ester storage are increased.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Prack MM,Rothblat GH,Erickson SK,Reyland ME,Williams DLdoi
10.1021/bi00183a007subject
Has Abstractpub_date
1994-05-03 00:00:00pages
5049-55issue
17eissn
0006-2960issn
1520-4995journal_volume
33pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Human type-alpha transforming growth factor (hTGF alpha) is a small mitogenic protein containing 50 amino acids and three disulfide bonds. It has both sequence and structural homology with epidermal growth factor (EGF). While the three-dimensional structures of hTGF alpha and other EGF-like proteins have been studied ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00008a003
更新日期:1995-02-28 00:00:00
abstract::Electron paramagnetic resonance (EPR) imaging utilizing stable nitroxyl radicals is a promising technique for measuring free radical distribution, metabolism, and tissue oxygenation in organs and tissues [Kuppusamy, P., Chzhan, M., Vij, K., Shteynbuk, M., Lefer, D. J., Giannella, E., & Zweier, J. L. (1994) Proc. Natl....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952857s
更新日期:1996-06-04 00:00:00
abstract::Mutation of Gly90, Glu113, Ala292, and Lys296 in the visual pigment rhodopsin constitutively activates the protein for activation of the G protein transducin. Three of these mutations have been shown to cause two different human diseases. Mutation of Gly90 and Ala292 results in complete night blindness, and mutation o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00037a035
更新日期:1995-09-19 00:00:00
abstract::In the course of measuring the concentrations of retinoic acids (RA) in bovine plasma, a major peak was observed which comigrated with 9-cis-RA on normal-phase high-performance liquid chromatography. Rechromatography of this retinoic on reverse-phase high-performance liquid chromatography showed that it was distinct f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00004a013
更新日期:1995-01-31 00:00:00
abstract::Saccharomyces cerevisiae tRNA and 5S RNA, Escherichia coli 5S RNA, and wheat germ 5S RNA have each been specifically spin-labeled at the 3'-terminal ribose to give morpholino-spin-labeled (MSL) RNAs. Enzymatic hydrolysis with pancreatic RNase, followed by anion-exchange chromatography, confirms the site of attachment ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00268a042
更新日期:1982-12-07 00:00:00
abstract::The effects of several guanine nucleotide analogues on (Na+,K+)-ATPase activity of membranes isolated from several tissues were analyzed to determine if a G-protein might be involved in the hormonal regulation of the (Na+,K+)-ATPase. Submillimolar concentrations of GTP gamma S, but not GMPPNP, inhibit rat skeletal mus...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00241a014
更新日期:1991-07-09 00:00:00
abstract::Sendai virus particles are able to fuse with Pronase-neuraminidase-treated human erythrocyte membranes as well as with vesicles obtained from chromaffin granules of bovine medulla. Fusion is inferred either from electron microscopic studies or from the observation that incubation of fluorescently labeled (bearing octa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00387a018
更新日期:1987-06-30 00:00:00
abstract::A chromatin acceptor protein for the avian oviduct progesterone receptor (PR), termed receptor binding factor 1 (RBF-1), has recently been shown to (1) be a component of the nuclear binding sites (acceptor sites) for PR and (2) generate high-affinity binding sites (termed the RBF-1 class of sites) on avian genomic DNA...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00103a019
更新日期:1991-10-01 00:00:00
abstract::β-2-Microglobulin (β2m) forms amyloid fibrils in the joints of patients undergoing dialysis treatment as a result of kidney failure. One of the ways in which β2m can be induced to form amyloid fibrils in vitro is via incubation with stoichiometric amounts of Cu(II). To better understand the structural changes caused b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b01198
更新日期:2017-02-28 00:00:00
abstract::Posttranslational glycosylation is critical for biological function of many proteins, but its structural characterization is complicated by natural heterogeneity, multiple glycosylation sites, and different forms. Here, a top-down mass spectrometry (MS) characterization is applied to three constructs of the Fc segment...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9919091
更新日期:2000-03-28 00:00:00
abstract::Previous studies have shown that murine macrophages immunostimulated with interferon gamma and Escherichia coli lipopolysaccharide synthesize NO2-, NO3-, and citrulline from L-arginine by oxidation of one of the two chemically equivalent guanido nitrogens. The enzymatic activity for this very unusual reaction was foun...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00424a003
更新日期:1988-11-29 00:00:00
abstract::RSC (remodel the structure of chromatin) is an essential chromatin remodeler of Saccharomyces cerevisiae that has been shown to have DNA translocase properties. We studied the DNA binding properties of a "trimeric minimal RSC" (RSCt) of the RSC chromatin remodeling complex and the effect of nucleotides on this interac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200837b
更新日期:2011-09-20 00:00:00
abstract::13C NMR has been used to demonstrate the metabolism of dilute solutions of labeled formaldehyde by Escherichia coli to methanol, formate, carbon dioxide, and several other unidentified metabolites which contain labeled CH2 groups. Aeration of bacterial suspensions within the spectrometer dramatically increased the rat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00298a017
更新日期:1984-01-31 00:00:00
abstract::Proteins have many distinct tertiary folds (Richardson, J. S. (1981) Adv. Prot. Chem. 34, 167-339). The term tertiary fold refers to the spatial organization of secondary structure elements (alpha-helices and beta-strands). It is not known when, in the process of protein folding, a native tertiary fold emerges. Here, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00174a021
更新日期:1994-03-01 00:00:00
abstract::Because of the lack of protein turnover in fiber cells of the ocular lens, Aquaporin 0 (AQP0), the most abundant membrane protein in the lens, undergoes extensive post-translational modification with fiber cell age. To map the distribution of modified forms of AQP0 within the lens, normal human lenses ranging in age f...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0496034
更新日期:2004-08-03 00:00:00
abstract::The effects of GDP and of aurodox (N-methylkirromycin) on the affinity of elongation factor Tu (EF-Tu) for aminoacyl-tRNA (aa-tRNA) have been quantified spectroscopically by using Phe-tRNA(Phe)-Fl8, a functionally active analogue of Phe-tRNA(Phe) with a fluorescein dye convalently attached to the s4U-8 base. The assoc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00459a014
更新日期:1990-02-20 00:00:00
abstract::The use of cefoxitin, a poor substrate of the RTEM beta-lactamase, has allowed the kinetic and spectroscopic characterization of a covalent acyl-enzyme intermediate in the enzyme-catalyzed reaction. The rate of reappearance of catalytic activity in an enzyme sample diluted from an incubation with cefoxitin is nearly i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00554a012
更新日期:1980-06-24 00:00:00
abstract::Electron spin polarized electron paramagentic resonance (ESP EPR) spectra were obtained with deuterated iron-removed photosynthetic bacterial reaction centers (RCs) to specifically investigate the effect of the rate of primary charge separation, metal-site occupancy, and H-subunit content on the observed P865+QA- char...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9630319
更新日期:1997-07-15 00:00:00
abstract::The alpha, beta, and gamma subunits of the receptor with high affinity for immunoglobulin E were isolated and their compositions assessed by direct amino acid analysis and by incorporation of radioactive precursors. The compositions show no unusual features other than a rather high content of tryptophan in the alpha c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00383a024
更新日期:1987-05-05 00:00:00
abstract::The composition of the molybdate-stabilized glucocorticoid receptor (GR) complex has been investigated with a monoclonal antibody against the steroid-binding Mr 94 000 (94K) GR protein. It was concluded that one antibody molecule binds one 94K GR molecule. This finding constituted the basis for calculating the number ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00344a041
更新日期:1985-11-05 00:00:00
abstract::The inhibitory potency of 3'-azido-3'-deoxythymidine 5'-triphosphate (AZTTP) against HIV-1 reverse transcriptase (HIV-1 RT) has been further evaluated. The results indicate that the previously reported low Ki values for AZTTP against HIV-1 RT (2.35 nM) are due neither to the to the direct tight binding of AZTTP to HIV...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00120a013
更新日期:1992-02-11 00:00:00
abstract::We review signaling by reactive oxygen species, which is emerging as a major physiological process. However, among the reactive oxygen species, H(2)O(2) best fulfills the requirements of being a second messenger. Its enzymatic production and degradation, along with the requirements for the oxidation of thiols by H(2)O...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi9020378
更新日期:2010-02-09 00:00:00
abstract::Phosphorus NMR spectroscopy is an important technique for the investigation of metabolism in tissues and intact organisms (including man). However, quantitation of the signals from an NMR experiment is difficult because it is not known from which regions of a cell metabolites are detected. It is generally believed tha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00439a003
更新日期:1989-06-27 00:00:00
abstract::To gain insight into the physiological function of phosphoinositide 3-kinase (PI 3-kinase) lipid products, this study examines the interactions of the D-3 phosphoinositides with profilin and the consequent effects on actin dynamics and phosphoinositide turnover. Profilin, a ubiquitous actin-regulating protein, plays a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961878z
更新日期:1996-11-05 00:00:00
abstract::The agouti-related protein gene (Agrp) plays an important role in body weight regulation. The mature human protein is a single polypeptide chain of 112 amino acid residues, consisting of an N-terminal acidic region and a unique C-terminal cysteine-rich domain. The disulfide structure of recombinant human AGRP was dete...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981082v
更新日期:1998-09-01 00:00:00
abstract::Porcine parathyroid gland slices were incubated with 3H-labeled amino acids in order to label tissue proteins. After incubation a crude hormonal extract was prepared and analyzed by chromatography on carboxymethylcellulose. Among the three radioactive peaks which were detected in the eluate, two were identified as par...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00687a018
更新日期:1975-08-12 00:00:00
abstract::The bidirectional transformation of a protein between its native water-soluble and integral transmembrane conformations is demonstrated for FraC, a hemolytic protein of the family of pore-forming toxins. In the presence of biological membranes, the water-soluble conformation of FraC undergoes a remarkable structural r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b01112
更新日期:2015-11-24 00:00:00
abstract::Misfolded proteins in the endoplasmic reticulum (ER) are degraded by N-terminal threonine proteases within the 26S proteasome. Each protease is formed by an activated beta subunit, beta5/X, beta1/Y, or beta2/Z, that exhibits chymotrypsin-like, peptidylglutamyl-peptide hydrolyzing, or trypsin-like activity, respectivel...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011322y
更新日期:2001-11-06 00:00:00
abstract::Splicing factor RBM10 and its close homologues RBM5 and RBM6 govern the splicing of oncogenes such as Fas, NUMB, and Bcl-X. The molecular architecture of these proteins includes zinc fingers (ZnFs) and RNA recognition motifs (RRMs). Three of these domains in RBM10 that constitute the RNA binding part of this splicing ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01242
更新日期:2018-03-13 00:00:00
abstract::Recent models for water oxidation in photosystem II postulate that the tyrosine Y(Z) radical, Y(Z)(*), abstracts both an electron and a proton from the Mn cluster during one or more steps in the catalytic cycle. This coupling of proton- and electron-transfer events is postulated to provide the necessary driving force ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992749w
更新日期:2000-05-30 00:00:00