Abstract:
:Phosphorus NMR spectroscopy is an important technique for the investigation of metabolism in tissues and intact organisms (including man). However, quantitation of the signals from an NMR experiment is difficult because it is not known from which regions of a cell metabolites are detected. It is generally believed that only metabolites free in the cytosol are observed. In this study a comparison of concentration measurements obtained by NMR and after freeze extraction was made in the normoxic and ischemic rat heart. The influence of ischemia was examined because of its potential effect on the level of phosphate metabolites in various compartments. The same fraction of ATP always appears visible to NMR, whereas inorganic phosphate is largely NMR invisible until after a period of ischemia and the phosphomonoesters are only partially observed early in ischemia.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Jeffrey FM,Storey CJ,Nunnally RL,Malloy CRdoi
10.1021/bi00439a003subject
Has Abstractpub_date
1989-06-27 00:00:00pages
5323-6issue
13eissn
0006-2960issn
1520-4995journal_volume
28pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The substrate analogue 2-(1-carboxy-2-phenylethyl)-4-phenylazophenol is a potent competitive inhibitor of carboxypeptidase A. Upon ligation to the active site, the azophenol moiety undergoes a shift of pKa from a value of 8.76 to a value of 4.9; this provides an index of the Lewis acidity of the active site zinc ion. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00358a028
更新日期:1986-05-20 00:00:00
abstract::Several unique protein folds that catalyze the hydrolysis of phosphodiester bonds have arisen independently in nature, including the PD(D/E)XK superfamily (typified by type II restriction endonucleases and many recombination and repair enzymes) and the HNH superfamily (found in an equally wide array of enzymes, includ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700418d
更新日期:2007-06-19 00:00:00
abstract::In the reaction center (RC) of the photosynthetic bacterium Rhodobacter sphaeroides, two ubiquinone molecules, QA and QB, play a pivotal role in the conversion of light energy into chemical free energy by coupling electron transfer to proton uptake. In native RCs, the transfer of an electron from QA to QB takes place ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700297b
更新日期:2007-04-17 00:00:00
abstract::Histidine decarboxylase (HDC) from Lactobacillus 30a forms a trimer around a central cavity or well. Three active sites are formed around the well at the interface of each of two adjacent molecules. HDC exhibits cooperative kinetics at pH 7.6 and can be described in terms of a two-state, T and R, model. At pH 4.8, pro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00018a009
更新日期:1995-05-09 00:00:00
abstract::The anaerobic global regulator FNR from Escherichia coli is a [4Fe-4S](2+) cluster-containing dimer that is inactivated by O(2) through disruption of the Fe-S cluster and conversion to the monomeric apoprotein. It was shown that apo-FNR is subject to ClpXP proteolysis, and two recognition sites, amino acids 5-11 and a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi2018688
更新日期:2012-06-26 00:00:00
abstract::We have examined the structure of newly repaired regions of chromatin in intact and permeabilized human cells following exposure to bleomycin (BLM). The average repair patch size (in permeabilized cells) was six to nine bases, following doses of 1-25 micrograms/mL BLM, and greater than 80% of the total repair synthesi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00484a020
更新日期:1990-08-14 00:00:00
abstract::The luminescence of Cu-substituted cytochrome c exhibits temperature-dependent decay modes and spectral shifts at temperatures below 80 K. By comparison with the data from metal-substituted porphyrins, we determined intramolecular rate parameters. The observed tripdoublet decay time is 13 +/- 1 mus at 77 K and the qua...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00583a005
更新日期:1979-08-07 00:00:00
abstract::The pH-dependent behavior of the ferric forms of two soluble truncations of Rhizobium meliloti FixL, FixL (heme and kinase domains, functional), and FixLN (heme domain) are examined by UV-visible, resonance Raman, and electron paramagnetic resonance spectroscopy. Global analysis of UV-visible data indicates that the p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981439v
更新日期:1998-09-29 00:00:00
abstract::Trypanosomes are purine-auxotrophic parasites that depend upon nucleoside hydrolase (NH) activity to salvage nitrogenous bases necessary for nucleic acid and cofactor synthesis. Nonspecific and purine-specific NHs have been widely studied, yet little is known about the 6-oxopurine-specific isozymes, although they are ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100697d
更新日期:2010-10-19 00:00:00
abstract::Modelin-5 isoforms were used to gain an insight into the effects of amidation on antimicrobial selectivity. When tested against Escherichia coli, amidation increased toxicity 10-fold (MIC = 31.25 μM) while showing limited increased hemolytic activity (2% lysis). Our results show that both the amidated and non-amidated...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201267v
更新日期:2011-12-20 00:00:00
abstract::In a family of proteins, often the three-dimensional structure has been experimentally determined only for one member or a few members of the family. Homology modeling can be used to model the structures of all other members of the family and thus allow comparison of these structures. This approach was applied to hete...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026729x
更新日期:2002-12-10 00:00:00
abstract::The interaction of the so-called fusion peptide of the human immunodeficiency virus gp41 envelope glycoprotein with the target cell membrane is believed to trigger the fusion process which allows the entry of the virus into the cell. Many studies on the interaction of the fusion peptide with biological membranes have ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi050382r
更新日期:2005-10-11 00:00:00
abstract::In order to gain insight into the structure of human parathyroid hormone (hPTH), four fragments [hPTH(1-34), hPTH(18-48), hPTH(28-48), and hPTH(53-84)], which cover all regions of the intact hormone, have been investigated by CD and NMR spectroscopy in combination with distance geometry, and restrained molecular dynam...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00173a010
更新日期:1994-02-22 00:00:00
abstract::The studies reported here were undertaken to determine whether the thrombin precursors prothrombin, prethrombin 1, prethrombin 2, and Meizo thrombin interact with the fluorescent, reversible thrombin inhibitor dansylarginine N,N-(3-ethyl-1,5-pentanediyl)amide (DAPA) [Nesheim, M. E., Prendergast, F. G., & Mann, K. G. (...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00539a003
更新日期:1982-05-11 00:00:00
abstract::The flavoprotein component (SiR-FP) of the sulfite reductase from Escherichia coli is an octamer containing one FAD and one FMN per polypeptide chain. SiR-FP60, a SiR-FP fragment starting with alanine-52, was overexpressed in E. coli and purified as a monomer. The N-terminal part of the native protein contains thus al...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9728699
更新日期:1998-04-28 00:00:00
abstract::Time-resolved spectroscopic studies of unfolded horse iron(II) cytochrome c have suggested that the imidazole side chains of His26 and His33 bind transiently to the heme iron on microsecond time scales, after photodissociation of a carbon monoxide ligand from the heme. Our studies of four variants of cytochrome c (hor...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011371a
更新日期:2002-01-29 00:00:00
abstract::14C-Labeled fluorodinitrobenzene and N-ethylmaleimide have been used as chemical probes of the conformational states of myosin induced by the binding of MgADP and MgATP. The results indicate that in the high-energy conformation, MMgADP-Pi, the essential thiols are protected from modification but their diminished react...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00643a005
更新日期:1977-11-29 00:00:00
abstract::The major covalently linked multimolecular D fragments found in plasmic digests of factor XIIIa cross-linked fibrin formed under physiological pH and ionic strength conditions consist of D dimers, D trimers, and D tetramers. These fragments are linked by epsilon-amino-gamma-glutamyllysine bonds in the carboxy-terminal...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00118a040
更新日期:1992-01-28 00:00:00
abstract::Mutagenized dockerin domains of endoglucanase CelD (type I) and of the cellulosome-integrating protein CipA (type II) were constructed by swapping residues 10 and 11 of the first or the second duplicated segment between the two polypeptides. These residues have been proposed to determine the specificity of cohesin-doc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011853m
更新日期:2002-02-19 00:00:00
abstract::The effects of metal ion binding on the optical spectroscopic properties and temperature stability of two single tryptophan mutants of chicken skeletal TnC, F78W and F154W, have been examined. The absence of tyrosine and other tryptophan residues allowed the unambiguous assignment of the spectral signal from the intro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982048j
更新日期:1999-09-14 00:00:00
abstract::Liver alcohol dehydrogenase (LADH) carboxymethylated at Cys-46 (CMLADH) forms two different ternary complexes with 4-trans-(N,N-dimethylamino)cinnamaldehyde (DACA). The complex with reduced nicotinamide adenine dinucleotide (NADH) is characterized by a 38-nm red shift of the long-wavelength pi, pi* transition to 436 n...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00321a085
更新日期:1984-12-18 00:00:00
abstract::Spectroscopic methods are used to probe the interactions of several anilinoacridine analogues with calf thymus DNA over a wide range of temperatures and sodium chloride concentrations. The structurally similar compounds m-AMSA, AMSA (both active as antitumor agents), and o-AMSA (inactive as an antitumor agent) have be...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00231a025
更新日期:1991-04-30 00:00:00
abstract::Mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol (Chol) have been used to examine the effects of cholesterol on the chain crystallization transitions and thermomechanical properties in phospholipid bilayer membranes. The mechanical properties--elastic moduli and level of tension at membrane rupture--w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00413a013
更新日期:1988-06-28 00:00:00
abstract::The proximal bond between the iron atom of the heme group and the N epsilon of histidine F8 in myoglobin (Mb) and hemoglobin (Hb) is presumed to be an important determinant of heme binding, protein structure, and oxygen binding. Here a system is described in which the proximal ligand is provided intermolecularly by th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00187a023
更新日期:1994-05-31 00:00:00
abstract::The electrostatic binding strength of water-soluble proteins having either an excess positive (cytochrome c) or negative (beta-lactoglobulin) electric charge to oppositely charged supported planar bilayers (SPBs) was studied as a function of the bilayer phase state (fluid or gel phase) by IR-ATR spectroscopy. The bila...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991564m
更新日期:1999-11-16 00:00:00
abstract::The crystal structure of fiddler crab collagenase complexed with the dimeric serine protease inhibitor ecotin at 2.5 A resolution reveals an extended cleft providing binding sites for at least 11 contiguous substrate residues. Comparison of the positions of nine intermolecular main chain hydrogen bonding interactions ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9617522
更新日期:1997-05-06 00:00:00
abstract::Triglyceride analogues were synthesized in which one of the primary acyl ester functions has been replaced by an alkyl group and the secondary acyl ester bond has been replaced by an acyl amino bond. The chain length at either position was varied, and both (R)- and (S)-enantiomers of each compound were synthesized. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00019a020
更新日期:1995-05-16 00:00:00
abstract::Proteins performing multiple biochemical functions are called "moonlighting proteins" or extreme multifunctional (EMF) proteins. Mitochondrial cytochrome c is an EMF protein that binds multiple partner proteins to act as a signaling molecule, transfers electrons in the respiratory chain, and acts as a peroxidase in ap...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00890
更新日期:2017-11-21 00:00:00
abstract::Myosin belongs to the family of motor proteins. Its interaction with actin coupled with hydrolysis of ATP is the molecular basis of muscle contraction. The head segment of myosin, called subfragment 1 (S1), contains the distinct binding sites for ATP and actin and responsible for the ATPase activity. The rate-limiting...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952565r
更新日期:1996-04-09 00:00:00
abstract::High-pressure 31P NMR was used for the first time to investigate the effects of pressure on the structure and dynamics of the phosphocholine headgroup in pure 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) multilamellar aqueous dispersions and in DPPC bilayers containing the positively charged form of the local an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00143a004
更新日期:1992-07-21 00:00:00