Reactivity of essential thiols of myosin. Chemical probes of the activated state.


:14C-Labeled fluorodinitrobenzene and N-ethylmaleimide have been used as chemical probes of the conformational states of myosin induced by the binding of MgADP and MgATP. The results indicate that in the high-energy conformation, MMgADP-Pi, the essential thiols are protected from modification but their diminished reactivity does not result from depletion of the reagent by reaction at nonessential thiols. The binding of MgADP to myosin exposes the essential thiols as reflected by an increased rate of their modification. The influence of the divalent cations Mg2+ and Ca2+ on the conformation of the M species has also been investigated. By monitoring the incorporation of fluorodinitrobenzene, the conformations of the M state in the presence of these cations can be clearly discerned.






Reisler E,Burke M,Harrington WF




Has Abstract


1977-11-29 00:00:00












  • Another role for CO with nitrogenase? CO stimulates hydrogen evolution catalyzed by variant Azotobacter vinelandii Mo-nitrogenases.

    abstract::A likely entry/exit path for nitrogenase substrates, products, and/or protons involves residues α277(Arg), α192(Ser), and α356(Gly), all of which are highly conserved among MoFe proteins from different organisms. The α192(Ser) and α277(Arg) residues form part of a hydrogen-bonded network that also involves α195(His), ...


    pub_type: 杂志文章


    authors: Fisher K,Hare ND,Newton WE

    更新日期:2014-10-07 00:00:00

  • Substituted NDP-MSH peptides paired with mutant melanocortin-4 receptors demonstrate the role of transmembrane 6 in receptor activation.

    abstract::The melanocortin-4 receptor (MC4R) is involved in regulating energy homeostasis and is a potential therapeutic target for obesity and cachexia. Molecular interactions between peptide ligands and MC4R have been studied in detail. Less is known regarding the role of these interactions in the mechanism of MC4R activation...


    pub_type: 杂志文章


    authors: Fleck BA,Ling N,Chen C

    更新日期:2007-09-18 00:00:00

  • Bioluminescent Probes for Imaging Biology beyond the Culture Dish.

    abstract::Bioluminescence with luciferase-luciferin pairs is an attractive method for surveying cells in live tissues and whole organisms. Recent advances in luciferin chemistry and luciferase engineering are further expanding the scope of the technology. It is now possible to spy on cells in a variety of deep tissues and visua...


    pub_type: 杂志文章,评审


    authors: Rathbun CM,Prescher JA

    更新日期:2017-10-03 00:00:00

  • Structure-based design of an indolicidin peptide analogue with increased protease stability.

    abstract::Indolicidin is an antimicrobial cationic peptide with broad-spectrum activity isolated from bovine neutrophils. An indolicidin analogue CP-11, ILKKWPWWPWRRK-NH(2), with improved activity against Gram-negative bacteria had increased positive charge and amphipathicity while maintaining the short length of the parent mol...


    pub_type: 杂志文章


    authors: Rozek A,Powers JP,Friedrich CL,Hancock RE

    更新日期:2003-12-09 00:00:00

  • Solubilization of the carcinogen nickel subsulfide and its interaction with deoxyribonucleic acid and protein.

    abstract::Significant concentrations (1-10 mM) of nickel(II) were found in solution after incubation of the potent carcinogen nickel subsulfide in 0.05 M Tris-HCl, pH 7.4, solutions containing DNA, rat liver microsomes, and NADPH. The presence of NADPH decreased the rate of solubilization of nickel subsulfide. The solubilized n...


    pub_type: 杂志文章


    authors: Lee JE,Ciccarelli RB,Jennette KW

    更新日期:1982-02-16 00:00:00

  • Structural and Mutagenesis Studies Evince the Role of the Extended Protuberant Domain of Ribosomal Protein uL10 in Protein Translation.

    abstract::The lateral stalk of ribosomes constitutes the GTPase-associated center and is responsible for recruiting translation factors to the ribosomes. The eukaryotic stalk contains a P-complex, in which one molecule of uL10 (formerly known as P0) protein binds two copies of P1/P2 heterodimers. Unlike bacterial uL10, eukaryot...


    pub_type: 杂志文章


    authors: Choi KA,Yang L,Lee KM,Yu CW,Banfield DK,Ito K,Uchiumi T,Wong KB

    更新日期:2019-09-10 00:00:00

  • Mechanism of glutamate transport in Escherichia coli B. 1. Proton-dependent and sodium ion dependent binding of glutamate to a glutamate carrier in the cytoplasmic membrane.

    abstract::Specific binding of glutamate to its carrier was investigated by using cytoplasmic membrane vesicles prepared from Escherichia coli B. The binding activity was specifically affected by the Na+ and H+ concentrations of the medium. Cytoplasmic membrane vesicles from the mutant strain 36-39 that is defective in the Na+-d...


    pub_type: 杂志文章


    authors: Fujimura T,Yamato I,Anraku Y

    更新日期:1983-04-12 00:00:00

  • NMR structural studies of a 15-mer DNA sequence from a ras protooncogene, modified at the first base of codon 61 with the carcinogen 4-aminobiphenyl.

    abstract::Proton NMR studies were conducted on the complementary 15-mer duplex d(5'-TACTCTTCTTGACCT).(5'-AGGTCAAGAAGAGTA) (designated as unmodified 15-mer duplex) spanning a portion of the mouse c-Ha-ras protooncogene centered around codon 61. Identical studies were carried out on the same sequence, after specific modification ...


    pub_type: 杂志文章


    authors: Cho BP,Beland FA,Marques MM

    更新日期:1992-10-13 00:00:00

  • Roles of Ile209 and Ile210 on the heme pocket structure and regulation of histidine kinase activity of oxygen sensor FixL from Rhizobium meliloti.

    abstract::FixL is a sensor histidine kinase having a heme-containing domain as an O(2) sensing site. In the study presented here, Ile209 and Ile210 located near the heme iron of the heme domain of Rhizobium meliloti FixL (RmFixL) were mutated, and the mutational effects on the regulation of the kinase activity and the heme pock...


    pub_type: 杂志文章


    authors: Mukai M,Nakamura K,Nakamura H,Iizuka T,Shiro Y

    更新日期:2000-11-14 00:00:00

  • L-685,458, an aspartyl protease transition state mimic, is a potent inhibitor of amyloid beta-protein precursor gamma-secretase activity.

    abstract::Progressive cerebral amyloid beta-protein (A beta) deposition is believed to play a central role in the pathogenesis of Alzheimer's disease (AD). Elevated levels of A beta(42) peptide formation have been linked to early-onset familial AD-causing gene mutations in the amyloid beta-protein precursor (A beta PP) and the ...


    pub_type: 杂志文章


    authors: Shearman MS,Beher D,Clarke EE,Lewis HD,Harrison T,Hunt P,Nadin A,Smith AL,Stevenson G,Castro JL

    更新日期:2000-08-01 00:00:00

  • Mechanism of the conformational transition of melittin.

    abstract::It is known that, while melittin at micromolar concentrations is unfolded under conditions of low ionic strength at neutral pH, it adopts a tetrameric alpha-helical structure under conditions of high ionic strength, at alkaline pH, or at high peptide concentrations. To understand the mechanism of the conformational tr...


    pub_type: 杂志文章


    authors: Goto Y,Hagihara Y

    更新日期:1992-01-28 00:00:00

  • Thermodynamics of ferredoxin binding to ferredoxin:NADP+ reductase and the role of water at the complex interface.

    abstract::The association of ferredoxin with ferredoxin:NADP+ reductase (both proteins from spinach chloroplasts) was characterized by isothermal titration calorimetry and fluorescence quenching titration. The formation of the complex is mainly driven by a positive entropy change (delta S = 125 +/- 8 J mol-1 K-1). The calorimet...


    pub_type: 杂志文章


    authors: Jelesarov I,Bosshard HR

    更新日期:1994-11-15 00:00:00

  • A method for the separation of hybrids of chromatographically identical oligomeric proteins. Use of 3,4,5,6-tetrahydrophthaloyl groups as a reversible "chromatographic handle".

    abstract::Hybridization experiments with variants of an oligomeric protein often provide important information regarding subunit structure, function, and interactions. In some systems, however, the variants are so similar electrophoretically and chromatographically that purification of individual hybrids is not feasible. Theref...


    pub_type: 杂志文章


    authors: Gibbons I,Schachman HK

    更新日期:1976-01-13 00:00:00

  • Structural requirements for formyl homooligopeptide chemoattractants.

    abstract::Using solution peptide synthesis, we have made three series of N alpha-formylated homooligopeptides, from the dipeptide to the heptapeptide, derived from L-methionine, L-norleucine, and S-methyl-L-cysteine and related to the chemotactic peptide N alpha-formylmethionylleucylphenylalanine. Compounds were prepared to det...


    pub_type: 杂志文章


    authors: Toniolo C,Bonora GM,Showell H,Freer RJ,Becker EL

    更新日期:1984-02-14 00:00:00

  • Delta T 14/Delta D 15 Azotobacter vinelandii ferredoxin I: creation of a new CysXXCysXXCys motif that ligates a [4Fe-4S] cluster.

    abstract::In clostridial-type ferredoxins, each of the two [4Fe-4S]2+/+ clusters receives three of its four ligands from a CysXXCysXXCys motif. Azotobacter vinelandii ferredoxin I (AvFdI) is a seven-iron ferredoxin that contains one [4Fe-4S]2+/+ cluster and one [3Fe-4S]+/0 cluster. During the evolution of the 7Fe azotobacter-ty...


    pub_type: 杂志文章


    authors: Kemper MA,Gao-Sheridan HS,Shen B,Duff JL,Tilley GJ,Armstrong FA,Burgess BK

    更新日期:1998-09-15 00:00:00

  • Kinetic parameters of metal-substituted leucine aminopeptidase from bovine lens.

    abstract::Leucine aminopeptidase (LAP) is a protease requiring two divalent metal cations per subunit for activity. Zn2+, Mg2+, and Co2+ metal-substituted forms of LAP have been prepared and investigated kinetically. Substitution of metal into the two binding sites independently resulted in the preparation of Zn2+Zn2+, Mg2+Zn2+...


    pub_type: 杂志文章


    authors: Allen MP,Yamada AH,Carpenter FH

    更新日期:1983-08-02 00:00:00

  • Disulfide Bridging Strategies in Viral and Nonviral Platforms for Nucleic Acid Delivery.

    abstract::Self-assembled nanostructures that are sensitive to environmental stimuli are promising nanomaterials for drug delivery. In this class, disulfide-containing redox-sensitive strategies have gained enormous attention because of their wide applicability and simplicity of nanoparticle design. In the context of nucleic aci...


    pub_type: 杂志文章


    authors: Dutta K,Das R,Medeiros J,Thayumanavan S

    更新日期:2021-01-11 00:00:00

  • Characterization of hemopexin and its interaction with heme by differential scanning calorimetry and circular dichroism.

    abstract::Hemopexin is a plasma glycoprotein that has two structural domains (I and II) and binds and transports heme particularly to liver cells. Differential scanning calorimetry (DSC) studies show that hemopexin is largely stabilized by heme, which binds exclusively to domain I. The melting temperature (Tm) of heme-hemopexin...


    pub_type: 杂志文章


    authors: Wu ML,Morgan WT

    更新日期:1993-07-20 00:00:00

  • The carboxyl-terminal region of factor IX is essential for its secretion.

    abstract::The carboxyl-terminal region of factor IX (residues 403-415) contains several natural mutations which result in mild to severe forms of hemophilia B. A battery of factor IX minigene expression vectors carrying various mutations in the C-terminal region were constructed and studied by transient expression assay using H...


    pub_type: 杂志文章


    authors: Kurachi S,Pantazatos DP,Kurachi K

    更新日期:1997-04-08 00:00:00

  • All three LDL receptor homology regions of the LDL receptor-related protein bind multiple ligands.

    abstract::The three complete human LDL receptor homology regions of the LDL receptor-related protein (sLRP2, sLRP3, and sLRP4) have been expressed in Pichia pastoris SMD1168 with constitutive coexpression of the receptor-associated protein (RAP). Each sLRP was purified to homogeneity after deglycosylation using a combination of...


    pub_type: 杂志文章


    authors: Croy JE,Shin WD,Knauer MF,Knauer DJ,Komives EA

    更新日期:2003-11-11 00:00:00

  • Cell-cell recognition: specific binding of Microciona sponge aggregation factor to homotypic cells and the role of calcium ions.

    abstract::Previous studies have established that the mechanism of species-specific sponge cell reaggregation in vitro involves at least three components: a species-specific aggregation factor, its cell surface receptors, and Ca2+ ions. Here we report about the binding of Microciona 125I-labeled aggregation factor to cells and t...


    pub_type: 杂志文章


    authors: Jumblatt JE,Schlup V,Burger MM

    更新日期:1980-03-04 00:00:00

  • Cruciform DNA binding protein in HeLa cell extracts.

    abstract::We have analyzed by band-shift assays HeLa cell protein-DNA interactions on a stable cruciform DNA molecule. The stable cruciform was formed by heteroduplexing the HindIII-SphI fragment of SV40 virus DNA that contains the origin of replication with a derivative mutant containing a heterologous substitution at the cent...


    pub_type: 杂志文章


    authors: Pearson CE,Ruiz MT,Price GB,Zannis-Hadjopoulos M

    更新日期:1994-11-29 00:00:00

  • Phospholipase A2 engineering. Structural and functional roles of the highly conserved active site residue aspartate-49.

    abstract::Site-directed mutagenesis and high-resolution two-dimensional (2D) proton nuclear magnetic resonance (NMR) were used to probe the structural and functional roles of a highly conserved residue, Asp-49, in the interfacial catalysis by bovine pancreatic phospholipase A2 (PLA2, overexpressed in Escherichia coli). Accordin...


    pub_type: 杂志文章


    authors: Li Y,Yu BZ,Zhu H,Jain MK,Tsai MD

    更新日期:1994-12-13 00:00:00

  • Alteration of the iron-sulfur cluster composition of Escherichia coli dimethyl sulfoxide reductase by site-directed mutagenesis.

    abstract::We have used site-directed mutagenesis to alter the [Fe-S] cluster composition of Escherichia coli dimethyl sulfoxide (DMSO) reductase (DmsABC). The electron-transfer subunit (DmsB) of this enzyme contains 16 Cys residues arranged in 4 groups (I-IV) which provide ligands to 4 [4Fe-4S] clusters [Cammack, R., & Weiner, ...


    pub_type: 杂志文章


    authors: Rothery RA,Weiner JH

    更新日期:1991-08-27 00:00:00

  • Domain organization and functional analysis of type IIS restriction endonuclease Eco31I.

    abstract::Type IIS restriction endonuclease Eco31I harbors a single HNH active site and cleaves both DNA strands close to its recognition sequence, 5'-GGTCTC(1/5). A two-domain organization of Eco31I was determined by limited proteolysis. Analysis of proteolytic fragments revealed that the N-terminal domain of Eco31I is respons...


    pub_type: 杂志文章


    authors: Jakubauskas A,Sasnauskas G,Giedriene J,Janulaitis A

    更新日期:2008-08-19 00:00:00

  • Engineering of the nonspecific phospholipase C from Bacillus cereus: replacement of glutamic acid-4 by alanine results in loss of interfacial catalysis and enhanced phosphomonoesterase activity.

    abstract::The nonspecific phospholipase C from Bacillus cereus is a zinc metalloenzyme that catalyzes the hydrolysis of phospholipids to yield diacylglycerol and a phosphate monoester. Glu-4 has been proposed as a potential candidate for the general base in the hydrolysis reaction and was shown to interact with the substrate he...


    pub_type: 杂志文章


    authors: Tan CA,Roberts MF

    更新日期:1998-03-24 00:00:00

  • Crystal structures of Acetobacter aceti succinyl-coenzyme A (CoA):acetate CoA-transferase reveal specificity determinants and illustrate the mechanism used by class I CoA-transferases.

    abstract::Coenzyme A (CoA)-transferases catalyze transthioesterification reactions involving acyl-CoA substrates, using an active-site carboxylate to form covalent acyl anhydride and CoA thioester adducts. Mechanistic studies of class I CoA-transferases suggested that acyl-CoA binding energy is used to accelerate rate-limiting ...


    pub_type: 杂志文章


    authors: Mullins EA,Kappock TJ

    更新日期:2012-10-23 00:00:00

  • Structural alignments of (+)- and (-)-trans-anti-benzo[a]pyrene-dG adducts positioned at a DNA template-primer junction.

    abstract::The structural features of a chemically modified DNA template strand may promote error-prone DNA synthesis during replication. The resulting higher incidence of mutations, in turn, can eventually lead to tumor initiation. Structural insights into this process can be monitored by studying chemically modified base adduc...


    pub_type: 杂志文章


    authors: Cosman M,Hingerty BE,Geacintov NE,Broyde S,Patel DJ

    更新日期:1995-11-21 00:00:00

  • Discovery of inhibitors of Bacillus anthracis primase DnaG.

    abstract::Primase DnaG is an essential bacterial enzyme that synthesizes short ribonucleotide primers required for chromosomal DNA replication. Inhibitors of DnaG can serve as leads for development of new antibacterials and biochemical probes. We recently developed a nonradioactive in vitro primase-pyrophosphatase assay to iden...


    pub_type: 杂志文章


    authors: Biswas T,Green KD,Garneau-Tsodikova S,Tsodikov OV

    更新日期:2013-10-01 00:00:00

  • Radiolabeling of natural adenosine triphosphatase inhibitor with phenyl (14C)isothiocyanate and study of its interaction with mitochondrial adenosine triphosphatase. Localization of inhibitor binding sites and stoichiometry of binding.

    abstract::The natural ATPase inhibitor (IF1) from beef heart mitochondria was labeled with phenyl (14C)isothiocyanate [(14C)PITC]. Chemical labeling by (14C)PITC does not modify the inhibitory properties of IF1, provided the number of residues of (14C)PITC bound per molecule of IF1 is lower than five to six, which corresponds t...


    pub_type: 杂志文章


    authors: Klein G,Satre M,Dianoux AC,Vignais PV

    更新日期:1980-06-24 00:00:00