Abstract：
:Protein L31 from the 50S ribosomal subunit of Escherichia coli was manually sequenced by the dansyl-Edman method. Owing to the availability of only small quantities of purified L31, sequencing methods were scaled down such that the entire primary structure could be determined with 700 microgram of protein. The techniques employed are described in detail. The protein consists of a single chain of 62 amino acids, with a calculated molecular weight of 6967. Four half-cystine residues were identified at positions 16, 18, 37, and 40. Evidence is presented that suggests that these residues form two disulfide bridges in the protein, as isolated.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Brosius Jdoi
10.1021/bi00596a020subject
Has Abstractpub_date
1978-02-07 00:00:00pages
501-8issue
3eissn
0006-2960issn
1520-4995journal_volume
17pub_type
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