Abstract：
:We have investigated the binding of a pure H1 histone, the mouse variant H1e, to a 214 bp fragment of DNA from pBR322. Binding was monitored by observing the effects of the protein on melting of the DNA and by a gel-mobility-shift assay. We found, using this highly purified system, that H1e protein binds preferentially and cooperatively to the GC-rich region of the DNA. A chemically synthesized peptide containing 25 residues, corresponding to a region of the carboxyl-terminal domain of H1e, shows the same sequence preference but does not exhibit cooperativity.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Wellman SE,Sittman DB,Chaires JBdoi
10.1021/bi00167a049subject
Has Abstractpub_date
1994-01-11 00:00:00pages
384-8issue
1eissn
0006-2960issn
1520-4995journal_volume
33pub_type
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