Abstract:
:Energy coupling factor (ECF) transporters take up micronutrients in Bacteria and Archaea. They consist of a membrane-embedded S-component that provides substrate specificity and a three-subunit ECF module that couples ATP hydrolysis to transport. The S-components ThiT (for thiamin) and NiaX (for niacin) from Lactococcus lactis form complexes with the same ECF module. Here, we assayed the uptake of thiamin and niacin in Escherichia coli cells expressing the transporter genes. We demonstrate that the two different S-components compete for the ECF module, and that competition is more efficient in the presence of the transported substrate. The data suggest that binding and release of the S-components is a step in the transport cycle.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Majsnerowska M,Ter Beek J,Stanek WK,Duurkens RH,Slotboom DJdoi
10.1021/acs.biochem.5b00609subject
Has Abstractpub_date
2015-08-11 00:00:00pages
4763-6issue
31eissn
0006-2960issn
1520-4995journal_volume
54pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::A human cDNA, UDPGTh-3, encoding a dihydrotestosterone/5 alpha-androstane-3 alpha,17 beta-diol UDP- glucuronosyltransferase (transferase) has been isolated and characterized. The nucleotide sequence of UDPGTh-3 encodes a 530 amino acid protein with a typical membrane insertion-signal peptide, a membrane-anchoring doma...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00091a015
更新日期:1993-10-12 00:00:00
abstract::Perturbations within the transactivation and carboxy-terminal domains of HIV-1 Rev were examined for effects on Rev responsive element (RRE) binding activities in vitro and biological activity in vivo. Binding affinities, specificities, and multimerization of the transactivation mutants M10 and Rev/Rex M10-16 on the R...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00085a028
更新日期:1993-08-31 00:00:00
abstract::Antisera were raised in rabbits against the 40-kDa alpha subunit of bovine lung GTP-binding protein, which were identified as the alpha subunit of Gi2 (Gi2 alpha) by the analysis of the partial amino acid sequence. Antibodies were purified with a Gi2 alpha-coupled Sepharose column and then were passed through a Gi1 al...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00437a035
更新日期:1989-05-30 00:00:00
abstract::In this paper, we report kinetic studies for the chymotryptic activity of the 20S proteasome. Major observations include the following: (1) Reaction progress curves that are recorded at concentrations of Suc-Leu-Leu-Val-Tyr-AMC greater than about 40 microM are biphasic and characterized by initial velocities that deca...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952262x
更新日期:1996-04-02 00:00:00
abstract::The conserved glycines in the glycine-rich loop (Leu-Gly50-Thr-Gly52-Ser-Phe-Gly55-Arg-Val) of the catalytic (C) subunit of cAMP-dependent protein kinase were each mutated to Ser (G50S, G52S, and G55S). The effects of these mutations were assessed here using both steady-state and pre-steady-state kinetic methods. Whil...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972987w
更新日期:1998-05-26 00:00:00
abstract::The inhibition of phosphorylase kinase catalytic activity by 0.1 M neutral salts was predicted by the Hofmeister series of anions. The site of action of the salts was determined by the following evidence to be on the phosphorylase kinase molecule directly, rather than on its protein substrate. (1) Nonactivated kinase ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00665a022
更新日期:1976-10-05 00:00:00
abstract::Homology modeling was used to identify two particular residues, Glu175 and Ala176, in Pseudomonas stutzeri phosphite dehydrogenase (PTDH) as the principal determinants of nicotinamide cofactor (NAD(+) and NADP(+)) specificity. Replacement of these two residues by site-directed mutagenesis with Ala175 and Arg176 both s...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035018b
更新日期:2003-10-14 00:00:00
abstract::Although the addition of various divalent metals to beta-galactosidase resulted in apparent activation, only Mg2+ and Mn2+ actually did activate. The apparent activation by the other divalent metals was shown to be due to Mg2+ impurities. Calcium did not activate, but experiments suggested that it did bind. Other diva...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00586a005
更新日期:1979-09-18 00:00:00
abstract::Two high molecular weight DNA polymerases, which we have designated delta I and delta II, have been purified from calf thymus tissue. Using Bio Rex-70, DEAE-Sephadex A-25, and DNA affinity resin chromatography followed by sucrose gradient sedimentation, we purified DNA polymerase delta I 1400-fold to a specific activi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00349a005
更新日期:1986-01-14 00:00:00
abstract::Misfolded proteins in the endoplasmic reticulum (ER) are degraded by N-terminal threonine proteases within the 26S proteasome. Each protease is formed by an activated beta subunit, beta5/X, beta1/Y, or beta2/Z, that exhibits chymotrypsin-like, peptidylglutamyl-peptide hydrolyzing, or trypsin-like activity, respectivel...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011322y
更新日期:2001-11-06 00:00:00
abstract::A method for the rapid and quantitative analysis of 5'-terminal oligonucleotides of RNAs made in vitro is described. The method involves synthesis of RNA in the presence of [gamma-32P]ATP or GTP, isolation of the RNA, and digestion with T1 or pancreatic ribonucleases to release labeled 5'-triphosphate termanated oligo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00604a005
更新日期:1978-05-30 00:00:00
abstract::Glucose oxidation to CO2 was investigated in isolated perifused rat epididymal fat cells. Insulin stimulated rates of oxidation up to 30-fold. Multiple pulses of insulin or prolonged perifusion with the hormone led to a time-dependent desensitization of the cells. The action of insulin could be mimicked by H2O2. Rever...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00273a013
更新日期:1983-02-15 00:00:00
abstract::The binding equilibrium of phospholipase A2 (PLA2) to the substrate interface influences many aspects of the overall kinetics of interfacial catalysis by this enzyme. For example, the interpretation of kinetic data on substrate specificity was difficult when there was a significant kinetic contribution from the interf...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00243a037
更新日期:1991-07-23 00:00:00
abstract::Cellular Ca2+ transients and Ca2+-binding proteins regulate physiological phenomena as diverse as muscle contraction, neurosecretion, and cell division. When Ca2+ is rapidly mixed with slow Ca2+ chelators, EGTA, or Mg2+/EDTA, artificial Ca2+ transients (ACTs) of varying duration (0.1-50 ms half-widths (hws)) and ampli...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982495z
更新日期:1999-03-30 00:00:00
abstract::We have investigated the interaction of alpha 2-macroglobulin (alpha 2M) with the serine proteinase urokinase, an activator of plasminogen. Urokinase formed sodium dodecyl sulfate stable complexes with purified alpha 2M and with alpha 2M in plasma. These complexes could be visualized after polyacrylamide gel electroph...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00336a014
更新日期:1985-07-16 00:00:00
abstract::Lysyl-5-fluoro-L-tryptophyllysine and lysyl-5-fluoro-D-tryptophyllysine were synthesized, and their interactions with double-stranded DNA were investigated as a model for protein-nucleic acid interactions. The binding to DNA was studied by monitoring various 19F NMR parameters, the fluorescence, and the optical absorb...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00337a012
更新日期:1985-07-30 00:00:00
abstract::Bactenecin 5 and its fragments [BN22 (1-22), BN16 (7-22), and BC24 (20-43)] were synthesized by solid-phase methods. Their antifungal activities on Candida albicans have been studied and compared with those of the native bactenecin 5. The conformational preferences of these peptides in aqueous and nonaqueous solutions...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951681r
更新日期:1996-04-09 00:00:00
abstract::The duplex formed by annealing the formacetal backbone modified dodecamer d-(CGCGTTOCH2OTTGCGC) to its complementary strand, d(GCGCAAAACGCG) (duplex I), has been studied by NMR techniques and analyzed with reference to its unmodified counterpart (duplex II). Comparison of parameters such as 2D cross-peak intensities, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00142a009
更新日期:1992-07-14 00:00:00
abstract::Sso7d from the thermoacidophilic archaebacterium Sulfolobus solfataricus is a small globular protein with a known three-dimensional structure. Inspection of the structure reveals that Phe31 is a member of the aromatic cluster forming the protein hydrophobic core, whereas Trp23 is located on the protein surface and its...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972994k
更新日期:1998-07-21 00:00:00
abstract::Protein amyloid fibrils are originally identified as pathological entities in a variety of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Recent studies have revealed that amyloid fibrils also serve as functional protein assemblies to fulfill a wide range of biological functions. Decip...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.9b01069
更新日期:2020-02-11 00:00:00
abstract::Previous studies have shown that murine macrophages immunostimulated with interferon gamma and Escherichia coli lipopolysaccharide synthesize NO2-, NO3-, and citrulline from L-arginine by oxidation of one of the two chemically equivalent guanido nitrogens. The enzymatic activity for this very unusual reaction was foun...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00424a003
更新日期:1988-11-29 00:00:00
abstract::Oxygen binding curves of sol-gel-encapsulated deoxy human adult hemoglobin (HbA) have previously revealed two distinct noncooperative populations with oxygen binding affinities approximately 1000 and 100 times lower than that of the high-affinity R state. The two populations which have been termed the low-affinity (LA...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048531d
更新日期:2004-11-02 00:00:00
abstract::The enzymes in the catechol meta-fission pathway have been studied for more than 50 years in several species of bacteria capable of degrading a number of aromatic compounds. In a related pathway, naphthalene, a toxic polycyclic aromatic hydrocarbon, is fully degraded to intermediates of the tricarboxylic acid cycle by...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00050
更新日期:2016-05-10 00:00:00
abstract::Islet-activating protein (IAP), pertussis toxin, is a hexameric protein composed of an A protomer and a B oligomer, the residual pentamer having such a subunit assembly that two different dimers, dimer 1 and dimer 2, are connected with each other by means of the smallest C subunit. Incubation of IAP with formaldehyde ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00354a025
更新日期:1986-03-25 00:00:00
abstract::Beta-1,4-galactosyltransferase (beta4Gal-T1) in the presence of manganese ion transfers galactose from UDP-galactose (UDP-Gal) to N-acetylglucosamine (GlcNAc) that is either free or linked to an oligosaccharide. Crystallographic studies on bovine beta4Gal-T1 have shown that the primary metal binding site is located in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049007+
更新日期:2004-10-05 00:00:00
abstract::LTbetaR is a member of the TNF receptor family of proteins. It binds to two different cell surface ligands, LIGHT, a homotypic trimer, and LTalpha1beta2, a heterotypic trimer. We have measured the affinities of the dimeric IgG fusion protein, LTbetaRIgG, and monomeric LTbetaR protein binding to both LIGHT and LTalpha1...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060210+
更新日期:2006-08-22 00:00:00
abstract::The kinetic mechanism of NADPH-cytochrome P450 oxidoreductase (P450R) has been determined for the model reaction with cytochrome c3+. Although initial velocity studies show parallel patterns, consistent with a classical (one-site) ping-pong mechanism that precludes the formation of a ternary NADPH-P450R-cytochrome c3+...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00206a002
更新日期:1994-10-11 00:00:00
abstract::Measurements of the magnetic field dependence of the longitudinal magnetic relaxation rates (NMRD profiles) of solvent protons and deuterons led to the discovery of two classes of solvent binding sites in Ca2+-Mn2+-concanavalin A (CMPL) [Koenig, S. H., Brown, R. D., III, & Brewer, C. F. (1985) Biochemistry (second of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00340a004
更新日期:1985-09-10 00:00:00
abstract::Shaker potassium (K+) channels normally lack intrasubunit and intersubunit disulfide bonds. However, disulfide bonds are formed between Shaker subunits in intact cells exposed to oxidizing conditions. Upon electrophoresis under nonreducing conditions, intersubunit disulfide bond formation was detected by the presence ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961083s
更新日期:1996-09-17 00:00:00
abstract::Amphiphiles respond both to polar and to nonpolar solvents. In this paper X-ray diffraction and osmotic stress have been used to examine the phase behavior, the structural dimensions, and the work of deforming the monolayer-lined aqueous cavities formed by mixtures of dioleoylphosphatidylethanolamine (DOPE) and dioleo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00453a010
更新日期:1990-01-09 00:00:00