Selectivity of RNA chain initiation in vitro. 1. Analysis of RNA initiations by two-dimensional thin-layer chromatography of 5'-triphosphate-labeled oligonucleotides.

abstract：:Two tetrapeptide sequence homologies between mouse epidermal growth factor precursor (mEGFP) and human follitropin (FSH) were revealed by a computer program that identifies identical residues among polypeptide sequences. The two tetrapeptides, Lys-Thr-Cys-Thr (KTCT) and Thr-Arg-Asp-Leu (TRDL), are present in the hormo...

journal_title：Biochemistry

authors： Sluss PM,Krystek SR Jr,Andersen TT,Melson BE,Huston JS,Ridge R,Reichert LE Jr

更新日期：1986-05-06 00:00:00

abstract：:X-ray absorption spectra are reported for the multi-Cu oxidase Rhus vernicifera laccase in oxidized and fully reduced forms and for laccase from which the type 2 Cu has been depleted (T2D). The structure of the Cu K edge for both preparations shows the presence of CuII and CuI in the oxidized and reduced states, respe...

journal_title：Biochemistry

authors： Woolery GL,Powers L,Peisach J,Spiro TG

更新日期：1984-07-17 00:00:00

abstract：:Drug substrates stimulate ATPase activity of the P-glycoprotein (P-gp) ATP-binding cassette drug pump by an unknown mechanism. Cross-linking analysis was performed to test if drug substrates stimulate P-gp ATPase activity by altering cross-talk at the first transmission interface linking the drug-binding [intracellula...

journal_title：Biochemistry

authors： Loo TW,Clarke DM

更新日期：2016-05-24 00:00:00

abstract：:Pyruvate dehydrogenase kinase 2 (PDK2) activity is enhanced by the dihydrolipoyl acetyltransferase core (E2 60mer) that binds PDK2 and a large number of its pyruvate dehydrogenase (E1) substrate. With E2-activated PDK2, K(+) at approximately 90 mM and Cl(-) at approximately 60 mM decreased the K(m) of PDK2 for ATP and...

journal_title：Biochemistry

authors： Bao H,Kasten SA,Yan X,Roche TE

更新日期：2004-10-26 00:00:00

abstract：:Fibroblast growth factors (FGFs) are mitogenic and chemotactic agents for a wide variety of cell types and play a primary role in the regulation of angiogenesis. Angiogenesis is involved in a variety of critical physiological events including organogenesis, wound healing, ischemic collateral circulation, and solid tum...

journal_title：Biochemistry

authors： Blaber M,DiSalvo J,Thomas KA

更新日期：1996-02-20 00:00:00

abstract：:Nephrocalcin (NC), a calcium-binding glycoprotein of 14,000 molecular weight as a monomer, is known to inhibit the growth of calcium oxalate monohydrate (COM) crystals in renal tubules. We have isolated NC from bovine kidney tissue and purified into four isoforms, fractions A-D. NC-A and NC-B strongly inhibit the grow...

journal_title：Biochemistry

authors： Mustafi D,Nakagawa Y

更新日期：1996-11-26 00:00:00

abstract：:We have prepared the following cadmium-113-substituted derivatives of bovine superoxide dismutase and recorded the nuclear magnetic resonance (NMR) spectrum of the cadmium: 2Cd(II), in which Cd(II) is presumed to bind to the Zn(II) site and the copper site is unoccupied, and 2Cd(II)--2Cu(I), which is analogous to the ...

journal_title：Biochemistry

authors： Bailey DB,Ellis PD,Fee JA

更新日期：1980-02-05 00:00:00

abstract：:Infrared spectroscopy has been used to monitor residual ordered structure in the denatured state of wild-type and two mutants of iso-1-cytochrome c. The technique used involves a careful digital subtraction procedure that removes spectral contributions from buffer, water vapor, and the denaturant guanidine hydrochlori...

journal_title：Biochemistry

authors： Bowler BE,Dong A,Caughey WS

更新日期：1994-03-08 00:00:00

abstract：:Retinoschisin (RS1) is a retina-specific secreted protein encoding a conserved discoidin domain sequence. As an adhesion molecule, RS1 preserves the retinal cell architecture and promotes visual signal transduction. In young males, loss-of-function mutations in the X-linked retinoschisis gene (RS1) cause X-linked reti...

journal_title：Biochemistry

authors： Kotova S,Vijayasarathy C,Dimitriadis EK,Ikonomou L,Jaffe H,Sieving PA

更新日期：2010-08-24 00:00:00

abstract：:An adenosine cyclic 3',5'-monophosphate (cAMP) dependent protein kinase has recently been shown to exist in Dictyostelium discoideum and to be developmentally regulated. In this report we have followed the chromatographic behavior of both the holoenzyme and its subunits. A cAMP-dependent holoenzyme could be obtained f...

journal_title：Biochemistry

authors： Rutherford CL,Vaughan RL,Cloutier MJ,Ferris DK,Brickey DA

更新日期：1984-09-25 00:00:00

abstract：:We have developed a homogeneous time-resolved fluorescence resonance energy transfer (FRET)-based assay that detects the formation of HIV-1 integrase (IN) dimers. The assay utilizes IN monomers that express two different epitope tags that are recognized by their respective antibodies, coupled to distinct fluorophores....

journal_title：Biochemistry

authors： Tsiang M,Jones GS,Hung M,Samuel D,Novikov N,Mukund S,Brendza KM,Niedziela-Majka A,Jin D,Liu X,Mitchell M,Sakowicz R,Geleziunas R

更新日期：2011-03-15 00:00:00

abstract：:The receptor for atrial natriuretic peptide (ANP) is a type-I transmembrane protein containing an extracellular ligand-binding domain, a single transmembrane sequence, an intracellular kinase-homologous domain, and a guanylate cyclase (GCase) domain. Binding of ANP to the extracellular domain causes activation of the ...

journal_title：Biochemistry

authors： Misono KS,Sivasubramanian N,Berkner K,Zhang X

更新日期：1999-01-12 00:00:00

abstract：:Internal fatty acylation of proteins is a recognized means of modifying biological behavior. Escherichia coli hemolysin A (HlyA), a toxic protein, is transcribed as a nontoxic protein and made toxic by internal acylation of two lysine residue epsilon-amino groups; HlyC catalyzes the acyl transfer from acyl-acyl carrie...

journal_title：Biochemistry

authors： Trent MS,Worsham LM,Ernst-Fonberg ML

更新日期：1999-07-06 00:00:00

abstract：:When alpha,beta-unsaturated substrates bind to the active site of enoyl-CoA hydratase, large spectral changes can be observed [D'Ordine, R. L., et al. (1994) Biochemistry 33, 12635-12643]. The differences in the isotropic magnetic shieldings of the free and active site-bound forms of the carbonyl, alpha-, and beta-car...

journal_title：Biochemistry

authors： D'Ordine RL,Pawlak J,Bahnson BJ,Anderson VE

更新日期：2002-02-26 00:00:00

abstract：:The members of the glutamine amidotransferase (GATase) family catalyze the incorporation of ammonia within numerous metabolic pathways and can be categorized in two classes. Here, we concentrated on class I GATases, which are heteromeric enzyme complexes consisting of synthase subunits and glutaminase subunits with a ...

journal_title：Biochemistry

authors： Semmelmann F,Hupfeld E,Heizinger L,Merkl R,Sterner R

更新日期：2019-06-04 00:00:00

abstract：:Conformational distributions of a four-way DNA junction have been examined by time-resolved fluorescence resonance energy transfer (FRET). A series of dye-labeled junctions were synthesized with donor (fluorescein) and acceptor (tetramethylrhodamine) dyes conjugated to the 5' termini of the duplex arms in all six pair...

journal_title：Biochemistry

authors： Eis PS,Millar DP

更新日期：1993-12-21 00:00:00

abstract：:The temperature dependence of the packing (order) and fluidity (microviscosity) of rabbit small, intestinal brush border vesicle membranes and of liposomes made from their extracted lipids has been investigated by using a variety of lipid spin probes. The lipids in the brush border membrane are present essentially as ...

journal_title：Biochemistry

authors： Hauser H,Gains N,Semenza G,Spiess M

更新日期：1982-10-26 00:00:00

abstract：:The calcitonin gene-related peptide (CGRP) receptor is a heterodimer of two membrane proteins: calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). CLR is a class B G-protein-coupled receptor (GPCR), possessing a characteristic large amino-terminal extracellular domain (ECD) impor...

journal_title：Biochemistry

authors： Koth CM,Abdul-Manan N,Lepre CA,Connolly PJ,Yoo S,Mohanty AK,Lippke JA,Zwahlen J,Coll JT,Doran JD,Garcia-Guzman M,Moore JM

更新日期：2010-03-09 00:00:00

abstract：:The guanidine hydrochloride- (GuHCl-) induced unfolding and refolding of a recombinant domain of bovine microsomal cytochrome b(5) containing the first 104 amino acid residues has been characterized by both transient and equilibrium spectrophotometric methods. The soluble domain is reversibly unfolded and the equilibr...

journal_title：Biochemistry

authors： Manyusa S,Mortuza G,Whitford D

更新日期：1999-10-26 00:00:00

abstract：:The three-dimensional chalice-like crystal structure of initiation factor 2 IF2/eIF5B from Methanobacterium thermoautotrophicum represents a novel fold and domain architecture in which the N-terminal G domain and the C-terminal C domain are separated by an approximately 40 A alpha-helix. Homologous Thermus thermophilu...

journal_title：Biochemistry

authors： Zoldák G,Sedlák E,Wolfrum A,Musatov A,Fedunová D,Szkaradkiewicz K,Sprinzl M

更新日期：2008-04-29 00:00:00

abstract：:The nature of the putative general acid His187 in the reaction catalyzed by Escherichia coli uracil DNA glycosylase (UDG) was investigated using X-ray crystallography and NMR spectroscopy. The crystal structures of H187Q UDG, and its complex with uracil, have been solved at 1.40 and 1.60 A resolution, respectively. Th...

journal_title：Biochemistry

authors： Drohat AC,Xiao G,Tordova M,Jagadeesh J,Pankiewicz KW,Watanabe KA,Gilliland GL,Stivers JT

更新日期：1999-09-14 00:00:00

abstract：:Retinoids function through conformational alterations of ligand-dependent nuclear transcription factors, the retinoic acid receptors, and retinoid X receptors. 9-cis-Retinoic acid is a known biological ligand for retinoid X receptors, but its synthesis pathway in vivo is largely unknown. Recently, we identified a cis-...

journal_title：Biochemistry

authors： Paik J,Vogel S,Piantedosi R,Sykes A,Blaner WS,Swisshelm K

更新日期：2000-07-11 00:00:00

abstract：:Escherichia coli 16S RNA from 30S ribosomal subunits was isolated, oxidized at the 3' end, and labeled with the thiosemicarbazide derivatives of fluorescein or eosin. Labeled 16S RNA was reconstituted into 30S subunits. They were almost fully active compared to 30S subunits reconstituted from unlabeled 16S RNA by usin...

journal_title：Biochemistry

authors： Robbins D,Odom OW Jr,Lynch J,Kramer G,Hardesty B,Liou R,Ofengand J

更新日期：1981-09-01 00:00:00

abstract：:A new class of eukaryotic protein kinases that are not homologous to members of the serine/threonine/tyrosine protein kinase superfamily was recently identified [Futey, L. M., et al. (1995) J. Biol. Chem. 270, 523-529; Ryazanov, A. G., et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 4884-4889]. This class includes euk...

journal_title：Biochemistry

authors： Pavur KS,Petrov AN,Ryazanov AG

更新日期：2000-10-10 00:00:00

abstract：:The dynamics of the backbone and (some of) the side chains of lac headpiece (1-56; lac HP56) have been studied for the free protein and for its complex with lac half-operator DNA by 15N T1 and T1p relaxation measurements combined with [1H-15N] NOE experiments. For the structurally well-defined part of the free lac HP5...

journal_title：Biochemistry

authors： Slijper M,Boelens R,Davis AL,Konings RN,van der Marel GA,van Boom JH,Kaptein R

更新日期：1997-01-07 00:00:00

abstract：:In mesophiles, triosephosphate isomerase (TIM) is an obligated homodimer. We have previously shown that monomeric folding intermediates are common in the chemical unfolding of TIM, where dissociation provides 75% of the overall conformational stability of the dimer. However, analysis of the crystallographic structure ...

journal_title：Biochemistry

authors： Tellez LA,Blancas-Mejia LM,Carrillo-Nava E,Mendoza-Hernández G,Cisneros DA,Fernández-Velasco DA

更新日期：2008-11-04 00:00:00

abstract：:alpha-Conotoxin GI is a snail toxin protein consisting of 13 amino acids cross-linked by 2 intramolecular disulfide bridges. This toxin is an antagonist of acetylcholine receptors. The native sequence has been synthesized, along with nine additional variants in which non-cysteine residues are replaced by alanine or th...

journal_title：Biochemistry

authors： Zhang RM,Snyder GH

更新日期：1991-11-26 00:00:00

abstract：:F0F1 ATP synthase harnesses a transmembrane electrochemical gradient for the production of ATP. When operated in reverse, this multiprotein complex catalyzes ATP hydrolysis. In bacteria, the ε subunit is involved in regulating this ATPase activity. Also, ε is essential for coupling ATP hydrolysis (or synthesis) to pro...

journal_title：Biochemistry

authors： Rodriguez AD,Dunn SD,Konermann L

更新日期：2014-06-24 00:00:00

abstract：:In the accompanying paper [Suzuki, A., Yamazaki, M., & Ito, T. (1996) Biochemistry 35, 5238-5244], we presented a quantitative phase diagram of actin filament ( (F-actin) described with the F-actin concentration and delta chi value which characterizes the affinity of F-actin with solvent. The phase diagram shows that ...

journal_title：Biochemistry

authors： Suzuki A,Ito T

更新日期：1996-04-23 00:00:00

abstract：:Enolase is a glycolytic metalloenzyme involved in carbon metabolism. The advantage of targeting enolase lies in its essentiality in many biological processes such as cell wall formation and RNA turnover and as a plasminogen receptor. We initially used a DARTS assay to identify enolase as a target in Escherichia coli. ...

journal_title：Biochemistry

authors： Krucinska J,Falcone E,Erlandsen H,Hazeen A,Lombardo MN,Estrada A,Robinson VL,Anderson AC,Wright DL

更新日期：2019-03-05 00:00:00