Refolding and characterization of a soluble ectodomain complex of the calcitonin gene-related peptide receptor.

abstract：:Biotin synthase (BioB) is an iron-sulfur dimeric enzyme which catalyzes the last step in biotin synthesis. The reaction consists of the introduction of a sulfur atom into dethiobiotin. It is shown here that BioB displays a significant cysteine desulfurase activity, providing it with the ability to mobilize sulfur from...

journal_title：Biochemistry

authors： Ollagnier-De-Choudens S,Mulliez E,Hewitson KS,Fontecave M

更新日期：2002-07-23 00:00:00

abstract：:The cyclic antimicrobial lipopeptide daptomycin is now frequently used as a first-line therapy in serious infections caused by multidrug-resistant Enterococcus faecium. Resistance to daptomycin in E. faecium is mediated by activation of the LiaFSR membrane stress response pathway. Deletion of liaR, encoding the respon...

journal_title：Biochemistry

authors： Davlieva M,Wu C,Zhou Y,Arias CA,Shamoo Y

更新日期：2018-12-11 00:00:00

abstract：:The noncovalent interaction of 2-aminodipyrido[1,2-a:3',2'-d]imidazole (Glu-P-2) and its derivatives, which are potent mutagens isolated from L-glutamic acid pyrolysate, with calf thymus DNA was studied by steady-state and nanosecond fluorescence spectroscopies. The fluorescence of these compounds exhibits static quen...

journal_title：Biochemistry

authors： Itoh M,Kurokawa H,Usui M,Ohno M,Shimoda E,Hashimoto Y,Shudo K

更新日期：1985-11-05 00:00:00

abstract：:The enzyme 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase (EC 4.1. 2.16) (KDO 8-P synthase) that catalyzes the condensation of D-arabinose 5-phosphate (A 5-P) with phosphoenolpyruvate (PEP) to give 3-deoxy-D-manno-octulosonic acid 8-phosphate (KDO 8-P) and inorganic phosphate (Pi) was inactivated by the thiol-m...

journal_title：Biochemistry

authors： Salleh HM,Patel MA,Woodard RW

更新日期：1996-07-09 00:00:00

abstract：:Crowding, which characterizes the interior of all living cells, has been shown to dramatically affect biochemical processes, leading to stabilization of compact morphologies, enhanced macromolecular associations, and altered reaction rates. Due to the crowding-mediated shift in binding equilibria toward association, c...

journal_title：Biochemistry

authors： Goobes R,Kahana N,Cohen O,Minsky A

更新日期：2003-03-04 00:00:00

abstract：:The ability of avian-specific linker histone H5, and the globular domains of H5 (GH5) and H1(0) (GH1(0), to self-associate either free in solution or when bound to DNA was investigated. All three proteins underwent a salt-dependent increase in turbidity that may be indicative of nonspecific interactions. Dithiobis(suc...

journal_title：Biochemistry

authors： Carter GJ,van Holde K

更新日期：1998-09-08 00:00:00

abstract：:The initial step of infection of blood cells with the human immunodeficiency virus, HIV, is the formation of a complex of the viral envelope protein gp120 and its human receptor CD4. We have examined structural features of recombinant soluble CD4 (sCD4) by chemical modification of arginine residues with hydroxyphenylg...

journal_title：Biochemistry

authors： Hager-Braun C,Tomer KB

更新日期：2002-02-12 00:00:00

abstract：:Phosphoenolthiopyruvate, the analogue of phosphoenolpyruvate in which the bridging oxygen of the phosphate ester is replaced by sulfur, has been synthesized from methyl acrylate and dimethyl (chlorothio)phosphonate. The compound is a substrate for alkaline phosphatase, pyruvate kinase, enolase, and phosphoenolpyruvate...

journal_title：Biochemistry

authors： Sikkema KD,O'Leary MH

更新日期：1988-02-23 00:00:00

abstract：:A rapid assay procedure was developed for cleavage of the N-terminal propeptides of procollagen. With the assay a neutral procollagen N-protease was purified about 300-fold from chick embryo tendon extract. The enzyme had an apparent molecular weight of 260 000 and a pH optimum of 7.4. Ca2+ was required for enzymic ac...

journal_title：Biochemistry

authors： Tuderman L,Kivirikko KI,Prockop DJ

更新日期：1978-07-25 00:00:00

abstract：:PT-ACRAMTU ([PtCl(en)(ACRAMTU-S)](NO(3))(2), en = ethane-1,2-diamine, ACRAMTU = 1-[2-(acridin-9-ylamino)ethyl]-1,3-dimethylthiourea) is a cytotoxic platinum-acridine conjugate previously shown to form adducts with the N3 endocyclic nitrogen of adenine in the DNA minor groove. This unusual observation and our prior det...

journal_title：Biochemistry

authors： Budiman ME,Bierbach U,Alexander RW

更新日期：2005-08-23 00:00:00

abstract：:The post-polyketide synthase (PKS) biosynthetic tailoring of macrolide antibiotics usually involves one or more oxidation reactions catalyzed by cytochrome P450 monooxygenases. As the specificities of members from this class of enzymes vary significantly among PKS gene clusters, the identification and study of new mac...

journal_title：Biochemistry

authors： Betlach MC,Kealey JT,Ashley GW,McDaniel R

更新日期：1998-10-20 00:00:00

abstract：:The activity of phospholipase A2 from snake venom to hydrolyze bilayers of phosphatidylcholines is greatly enhanced by the presence of the hydrolysis products, lysolecithin and fatty acid, in the bilayer. The fluorescence of several probes of membrane structure was used to monitor changes in bilayer physical propertie...

journal_title：Biochemistry

authors： Sheffield MJ,Baker BL,Li D,Owen NL,Baker ML,Bell JD

更新日期：1995-06-20 00:00:00

abstract：:A description of the efficient high-level expression of the monomer hemoglobin (GMG4) from Glycera dibranchiata is presented. The cDNA described by Simons and Satterlee [Simons, P.C., & Satterlee, J.D. (1989) Biochemistry 28, 8525-8530] was subcloned into an expression system, and conditions were found that led to the...

journal_title：Biochemistry

authors： Alam SL,Dutton DP,Satterlee JD

更新日期：1994-08-30 00:00:00

abstract：:To better understand the mechanism of nitric oxide (NO) synthesis, we studied conversion of N-hydroxy-L-arginine (NOHA) or L-arginine (Arg) to citrulline and NO under single-turnover conditions using the oxygenase domain of neuronal nitric oxide synthase (nNOSoxy) and rapid scanning stopped-flow spectroscopy. When ana...

journal_title：Biochemistry

authors： Boggs S,Huang L,Stuehr DJ

更新日期：2000-03-07 00:00:00

abstract：:Calmodulin forms a covalent, one to one, complex with 3H-labeled norchlorpromazine isothiocyanate. Complex formation was monitored by high-performance liquid chromatography using a CN reverse-phase column which resolves calmodulin, the calmodulin-norchlorpromazine adduct, and norchlorpromazine isothiocyanate. Formatio...

journal_title：Biochemistry

authors： Newton DL,Burke TR Jr,Rice KC,Klee CB

更新日期：1983-11-22 00:00:00

abstract：:Cdc31, the Saccharomyces cerevisiae centrin, is an EF-hand calcium-binding protein essential for the cell division and mRNA nuclear export. We used biophysical techniques to investigate its calcium, magnesium, and protein target binding properties as well as their conformations in solution. We show here that Cdc31 dis...

journal_title：Biochemistry

authors： Miron S,Durand D,Chilom C,Pérez J,Craescu CT

更新日期：2011-07-26 00:00:00

abstract：:Guanidinium chloride- (GdmCl-) induced unfolding of beta-lactamase has been investigated by a combination of size-exclusion chromatography (SEC-FPLC) and usual optical methods. It has been shown that at low temperatures this protein unfolds through two equilibrium intermediates. The first of these intermediates is the...

journal_title：Biochemistry

authors： Uversky VN,Ptitsyn OB

更新日期：1994-03-15 00:00:00

abstract：:Human ecto-ATPase (E-NTPDase 2) and chicken ecto-ATP-diphosphohydrolase (E-NTPDase 8) are cell surface nucleotidases with two transmembranous domains, one each at the N- and C-termini. Hydrolysis of substrates occurs in active sites residing in their extracellular domains. Human ecto-ATPase activity is decreased by NP...

journal_title：Biochemistry

authors： Mukasa T,Lee Y,Knowles AF

更新日期：2005-08-23 00:00:00

abstract：:New isoforms of CYP51 (sterol 14alpha-demethylase), an essential enzyme in sterol biosynthesis and primary target of azole antimycotic drugs, are found in pathogenic protists, Trypanosoma brucei(TB), T. vivax, T. cruzi, and Leishmania major. The sequences share approximately 80% amino acid identity and are approximate...

journal_title：Biochemistry

authors： Lepesheva GI,Nes WD,Zhou W,Hill GC,Waterman MR

更新日期：2004-08-24 00:00:00

abstract：:Purple membranes of Halobacterium halobium were studied by differential scanning calorimetry. No transition was detected at temperatures below 70 degrees C. A small endothermic transition was seen at about 80 degrees C and a larger one at 100 degrees C. The larger transition is the irreversible denaturation of bacteri...

journal_title：Biochemistry

authors： Jackson MB,Sturtevant JM

更新日期：1978-03-07 00:00:00

abstract：:The flexibility of individual amino acid side chains of pancreatic phospholipase A2 in aqueous and micellar solutions was studied with deuterium nuclear magnetic resonance (2H NMR). Bovine pancreatic phospholipase A2 was selectively deuterated at the aromatic ring systems of Trp-3 and Phe-5 and porcine pancreatic phos...

journal_title：Biochemistry

authors： Allegrini PR,van Scharrenburg GJ,Slotboom AJ,de Haas GH,Seelig J

更新日期：1985-06-18 00:00:00

abstract：:A detailed study has been made of the polyacrylamide gel electrophoresis of DNA restriction fragments obtained from two plasmids, pBR322 and p82-6B. Variables studied were molecular weight, gel concentration, temperature, and electric field strength. The retardation coefficients of the larger fragments (greater than 8...

journal_title：Biochemistry

authors： Stellwagen NC

更新日期：1983-12-20 00:00:00

abstract：:The role of lateral diffusion in mitochondrial electron transport has been investigated by measuring the diffusion coefficients for lipid, cytochrome c, and cytochrome oxidase in membranes of giant mitoplasts from cuprizone-fed mice using the technique of fluorescence redistribution after photobleaching (FRAP). The di...

journal_title：Biochemistry

authors： Hochman J,Ferguson-Miller S,Schindler M

更新日期：1985-05-07 00:00:00

abstract：:The formation of pseudouridine (psi) on U5 small nuclear RNA (U5 snRNA) was studied using an in vitro modification system. Labeled U5 RNA, synthesized in vitro and therefore unmodified, was incubated in reactions containing S100 and/or nuclear extracts (NE) from HeLa cells, and the levels of psi were determined. There...

journal_title：Biochemistry

authors： Patton JR

更新日期：1994-08-30 00:00:00

abstract：:The relative roles of insulin and glucose in the regulation of hepatic glycogen synthase and phosphorylase were studied in hepatocytes from fed rats. Elevation of extra-cellular glucose led to a rapid decrease in phosphorylase a activity followed by a slower increase in glycogen synthase I activity. A reciprocal and c...

journal_title：Biochemistry

authors： Witters LA,Avruch J

更新日期：1978-02-07 00:00:00

abstract：:Aminoacylation of a transfer RNA (tRNA) by its cognate aminoacyl-tRNA synthetase relies upon the recognition of specific nucleotides as well as conformational features within the tRNA by the synthetase. In Escherichia coli, the aminoacylation of tRNA(His) by histidyl-tRNA synthetase (HisRS) is highly dependent upon th...

journal_title：Biochemistry

authors： Connolly SA,Rosen AE,Musier-Forsyth K,Francklyn CS

更新日期：2004-02-03 00:00:00

abstract：:The fibrillation of misfolded neurodegenerative disease-related proteins has been extensively studied over the past few decades, but the initial oligomerization has been rarely examined even though some recent evidence indicated that small protein oligomers are more neurotoxic than long protein fibers. It is rather di...

journal_title：Biochemistry

authors： Wang Y,Wu C

更新日期：2017-12-19 00:00:00

abstract：:The crystal structure of the heme-copper oxidases suggested a putative channel of oxygen entry into the heme-copper site of O(2) reduction. Changing a conserved valine near this center in cytochrome bo(3) of Escherichia coli to isoleucine caused a significant increase in the apparent K(M) for oxygen with little or no ...

journal_title：Biochemistry

authors： Riistama S,Puustinen A,Verkhovsky MI,Morgan JE,Wikström M

更新日期：2000-05-30 00:00:00

abstract：:To understand the stabilization, folding, and functional mechanisms of proteins, it is very important to understand the structural and thermodynamic properties of the molten globule state. In this study, the global structure of the acid molten globule state, which we call MG1, of horse cytochrome c at low pH and high ...

journal_title：Biochemistry

authors： Nakamura S,Seki Y,Katoh E,Kidokoro S

更新日期：2011-04-19 00:00:00

abstract：:Resonance Raman spectroscopy is employed to characterize heme site structural changes arising from conformational heterogeneity in deoxyMb and ligated derivatives, i.e., the ferrous CO (MbCO) and ferric cyanide (MbCN) complexes. The spectra for the reversed forms of these derivatives have been extracted from the spect...

journal_title：Biochemistry

authors： Rwere F,Mak PJ,Kincaid JR

更新日期：2008-12-02 00:00:00