Resonance Raman interrogation of the consequences of heme rotational disorder in myoglobin and its ligated derivatives.


:Resonance Raman spectroscopy is employed to characterize heme site structural changes arising from conformational heterogeneity in deoxyMb and ligated derivatives, i.e., the ferrous CO (MbCO) and ferric cyanide (MbCN) complexes. The spectra for the reversed forms of these derivatives have been extracted from the spectra of reconstituted samples. Dramatic changes in the low-frequency spectra are observed, where newly observed RR modes of the reversed forms are assigned using protohemes that are selectively deuterated at the four methyl groups or at the four methine carbons. Interestingly, while substantial changes in the disposition of the peripheral vinyl and propionate groups can be inferred from the dramatic spectral shifts, the bonds to the internal histidyl imidazole ligand and those of the Fe-CO and Fe-CN fragments are not significantly affected by the heme rotation, as judged by lack of significant shifts in the nu(Fe-N(His)), nu(Fe-C), and nu(C-O) modes. In fact, the apparent lack of an effect on these key vibrational parameters of the Fe-N(His), Fe-CO, and Fe-CN fragments is entirely consistent with previously reported equilibrium and kinetic studies that document virtually identical functional properties for the native and reversed forms.






Rwere F,Mak PJ,Kincaid JR




Has Abstract


2008-12-02 00:00:00












  • Transcription inhibition of oncogenic KRAS by a mutation-selective peptide nucleic acid conjugated to the PKKKRKV nuclear localization signal peptide.

    abstract::Mutations in the Kirsten ras (KRAS) gene are present in almost all pancreatic adenocarcinomas, and one common mutation is at codon 12: GGT (Gly) is transformed into GAT (Asp). In this work we have targeted the KRAS coding sequence embracing the GAT mutation with a sense PNA molecule (P14), with the aim of downregulati...


    pub_type: 杂志文章


    authors: Cogoi S,Codognotto A,Rapozzi V,Meeuwenoord N,van der Marel G,Xodo LE

    更新日期:2005-08-09 00:00:00

  • Structure and action of heteronemertine polypeptide toxins. Membrane penetration by Cerebratulus lacteus toxin A-III.

    abstract::The heteronemertine worm Cerebratulus lacteus produces a family of three structurally homologous proteins that function as direct lytic factors for a variety of cells [Kem, W. R., & Blumenthal, K. M. (1978) J. Biol. Chem. 253, 5752-5757]. It is demonstrated herein that the hemolytic activity of the most abundant varia...


    pub_type: 杂志文章


    authors: Blumenthal KM

    更新日期:1982-08-31 00:00:00

  • NG-methyl-L-arginine functions as an alternate substrate and mechanism-based inhibitor of nitric oxide synthase.

    abstract::NG-Methyl-L-arginine (L-NMA) is one of the most commonly used inhibitors of the nitric oxide synthases (NOS). Results reported here demonstrate that L-NMA is an alternate substrate and a mechanism-based inhibitor of the inducible NOS purified from murine macrophages. The irreversible inhibition displays pseudo-first-o...


    pub_type: 杂志文章


    authors: Olken NM,Marletta MA

    更新日期:1993-09-21 00:00:00

  • Affinity labeling of Escherichia coli phenylalanyl-tRNA synthetase at the binding site for tRNAPhe.

    abstract::Periodate-oxidized tRNA(Phe) (tRNA(oxPhe)) behaves as a specific affinity label of tetrameric Escherichia coli phenylalanyl-tRNA synthetase (PheRS). Reaction of the alpha 2 beta 2 enzyme with tRNA(oxPhe) results in the loss of tRNAPhe aminoacylation activity with covalent attachment of 2 mol of tRNA dialdehyde/mol of ...


    pub_type: 杂志文章


    authors: Hountondji C,Schmitter JM,Beauvallet C,Blanquet S

    更新日期:1987-08-25 00:00:00

  • Serine protease of hepatitis C virus expressed in insect cells as the NS3/4A complex.

    abstract::Hepatitis C virus (HCV) protease NS3 and its protein activator NS4A participate in the processing of the viral polyprotein into its constituent nonstructural proteins. The NS3/4A complex is thus an attractive target for antiviral therapy against HCV. We expressed the full-length NS3 and NS4A in insect cells as a solub...


    pub_type: 杂志文章


    authors: Sali DL,Ingram R,Wendel M,Gupta D,McNemar C,Tsarbopoulos A,Chen JW,Hong Z,Chase R,Risano C,Zhang R,Yao N,Kwong AD,Ramanathan L,Le HV,Weber PC

    更新日期:1998-03-10 00:00:00

  • Circular substrates of the hammerhead ribozyme shift the internal equilibrium further toward cleavage.

    abstract::To test whether the Y-shaped conformation of the hammerhead ribozyme is maintained throughout the catalytic pathway, the cleavage properties of circular substrates which bind the ribozyme through helices I and II were determined. Constraining the position of helices I and II in this manner did not significantly alter ...


    pub_type: 杂志文章


    authors: Stage-Zimmermann TK,Uhlenbeck OC

    更新日期:1998-06-30 00:00:00

  • Slow binding inhibition of S-adenosylmethionine synthetase by imidophosphate analogues of an intermediate and product.

    abstract::S-Adenosylmethionine (AdoMet) synthetase catalyzes the only known route of biosynthesis of the primary in vivo alkylating agent. Inhibitors of this enzyme could provide useful modifiers of biological methylation and polyamine biosynthetic processes. The AdoMet synthetase catalyzed reaction converts ATP and L-methionin...


    pub_type: 杂志文章


    authors: Reczkowski RS,Markham GD

    更新日期:1999-07-13 00:00:00

  • The Display of Single-Domain Antibodies on the Surfaces of Connectosomes Enables Gap Junction-Mediated Drug Delivery to Specific Cell Populations.

    abstract::Gap junctions, transmembrane protein channels that directly connect the cytoplasm of neighboring cells and enable the exchange of molecules between cells, are a promising new frontier for therapeutic delivery. Specifically, cell-derived lipid vesicles that contain functional gap junction channels, termed Connectosomes...


    pub_type: 杂志文章


    authors: Gadok AK,Zhao C,Meriwether AI,Ferrati S,Rowley TG,Zoldan J,Smyth HDC,Stachowiak JC

    更新日期:2018-01-09 00:00:00

  • Probing the diphosphoglycerate binding pocket of HbA and HbPresbyterian (beta 108Asn --> Lys).

    abstract::HbPresbyterian (beta 108Asn --> Lys, HbP) contains an additional positive charge (per alpha beta dimer) in the middle of the central cavity and exhibits a lower oxygen affinity than wild-type HbA in the presence of chloride. However, very little is known about the molecular origins of its altered functional properties...


    pub_type: 杂志文章


    authors: Gottfried DS,Manjula BN,Malavalli A,Acharya AS,Friedman JM

    更新日期:1999-08-31 00:00:00

  • Specificity of lipid incorporation is determined by sequences in the N-terminal 37 of apoB.

    abstract::The N-terminal 17% of apolipoprotein B (apoB-17) is secreted lipid-poor while apoB-41 particles are secreted with a triacylglycerol (TAG)-rich core. Thus, the sequence between apoB-17 and apoB-41 is necessary for the assembly of TAG-rich lipoproteins. To delineate this region, C127 cells were permanently transfected t...


    pub_type: 杂志文章


    authors: Carraway M,Herscovitz H,Zannis V,Small DM

    更新日期:2000-08-15 00:00:00

  • Chemical modification of the bovine mitochondrial bc1 complex reveals critical acidic residues involved in the proton pumping activity.

    abstract::Bovine heart ubiquinol-cytochrome c reductase (bc1 complex) was modified with N-(ethoxycarbonyl)-2-ethoxy-1,2-dihydroquinoline (EEDQ), which is a selective reagent for buried carboxyl groups. EEDQ treatment caused a loss of the proton pumping activity of liposome-reconstituted bc1 complex, without effect on the passiv...


    pub_type: 杂志文章


    authors: Cocco T,Di Paola M,Papa S,Lorusso M

    更新日期:1998-02-17 00:00:00

  • Is Arg418 the catalytic base required for the hydrolysis step of the IMP dehydrogenase reaction?

    abstract::The first committed step of guanine nucleotide biosynthesis is the oxidation of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) catalyzed by IMP dehydrogenase. The reaction involves the reduction of NAD(+) with the formation of a covalent enzyme intermediate (E-XMP). Hydrolysis of E-XMP requires th...


    pub_type: 杂志文章


    authors: Guillén Schlippe YV,Hedstrom L

    更新日期:2005-09-06 00:00:00

  • 2- and 8-azido photoaffinity probes. 2. Studies on the binding process of 2-5A synthetase by photosensitive ATP analogues.

    abstract::The photoaffinity probes [gamma-32P]2-azidoATP (2-N3ATP) and [alpha-32P]8-azido-ATP (8-N3ATP) were used to investigate the binding of ATP to highly purified 2-5A synthetase. 2-N3ATP and 8-N3ATP are substrates for 2-5A synthetase [Suhadolnik, R.J., Karikó, K., Sobol, R.W., Jr., Li, S.W., Reichenbach, N.L., & Haley, B.E...


    pub_type: 杂志文章


    authors: Suhadolnik RJ,Li SW,Sobol RW Jr,Haley BE

    更新日期:1988-11-29 00:00:00

  • Biosynthesis of the carbohydrate units of immunoglobulins. 1. Purification and properties of galactosyltransferases from swine mesentary lymph nodes.

    abstract::Galactosyltransferase (UDPgalactose:glycoprotein galactosyltransferase EC was isolated from swine mesentary lymhromatography on Sepharose 4B colums containing covalently bound p-aminophenyl-beta-D-N-acetylglucosamine. The homogenous enzyme showed a single band on disc gel electrophoresis and had a specific a...


    pub_type: 杂志文章


    authors: Rao AK,Garver F,Mendicino J

    更新日期:1976-11-16 00:00:00

  • Purification, characterization, and investigation of the mechanism of aminoglycoside 3'-phosphotransferase type Ia.

    abstract::Aminoglycoside 3'-phosphotransferases [APH(3')s] are the most common cause of bacterial high-level resistance to aminoglycoside antibiotics in clinical isolates. A one-step affinity chromatography was used to purify APH(3') type Ia. The kinetic parameters for turnover of seven aminoglycosides and the corresponding min...


    pub_type: 杂志文章


    authors: Siregar JJ,Miroshnikov K,Mobashery S

    更新日期:1995-10-03 00:00:00

  • Structural basis for light activation of a chloroplast enzyme: the structure of sorghum NADP-malate dehydrogenase in its oxidized form.

    abstract::Some key chloroplast enzymes are activated by light via a ferredoxin-thioredoxin reduction system which reduces disulfide bridges in the enzymes. We describe for the first time the structural basis for the redox activation of a chloroplast enzyme, the NADP-dependent malate dehydrogenase (MDH) from Sorghum vulgare whos...


    pub_type: 杂志文章


    authors: Johansson K,Ramaswamy S,Saarinen M,Lemaire-Chamley M,Issakidis-Bourguet E,Miginiac-Maslow M,Eklund H

    更新日期:1999-04-06 00:00:00

  • Mutation of the conserved domains of two inositol polyphosphate 5-phosphatases.

    abstract::Two short amino acid motifs, WXGDXNXR and PXWCDRXL, define a large family of inositol polyphosphate 5-phosphatases. We tested the importance of seven of these conserved amino acids to substrate binding and catalysis by mutating each to alanine in the platelet 75 kDa inositol polyphosphate 5-phosphatase II (5-phosphata...


    pub_type: 杂志文章


    authors: Jefferson AB,Majerus PW

    更新日期:1996-06-18 00:00:00

  • Design and chemical synthesis of a magnetic resonance contrast agent with enhanced in vitro binding, high blood-brain barrier permeability, and in vivo targeting to Alzheimer's disease amyloid plaques.

    abstract::Molecular imaging is an important new direction in medical diagnosis; however, its success is dependent upon molecular probes that demonstrate selective tissue targeting. We report the design and chemical synthesis of a derivative of human amyloid-beta (Abeta) peptide that is capable of selectively targeting individua...


    pub_type: 杂志文章


    authors: Poduslo JF,Curran GL,Peterson JA,McCormick DJ,Fauq AH,Khan MA,Wengenack TM

    更新日期:2004-05-25 00:00:00

  • Iron binding to horse spleen apoferritin: a vanadyl ENDOR spin probe study.

    abstract::The role of the protein shell in the formation of the hydrous ferric oxide core of ferritin is poorly understood. A VO2+ spin probe study was undertaken to characterize the initial complex of Fe2+ with horse spleen apoferritin (96% L-subunits). A competitive binding study of VO2+ and Fe2+ showed that the two metals co...


    pub_type: 杂志文章


    authors: Hanna PM,Chasteen ND,Rottman GA,Aisen P

    更新日期:1991-09-24 00:00:00

  • Bis(pyrrolecarboxamide) linked to intercalating chromophore oxazolopyridocarbazole (OPC): selective binding to DNA and polynucleotides.

    abstract::We have investigated some properties related to interaction with DNA and recognition of AT-rich sequences of netropsin-oxazolopyridocarbazole (Net-OPC) (Mrani et al., 1990), which is a hybrid groove-binder-intercalator. The hybrid molecule Net-OPC binds to poly[d(A-T)] at two different sites with Kapp values close to ...


    pub_type: 杂志文章


    authors: Subra F,Carteau S,Pager J,Paoletti J,Paoletti C,Auclair C,Mrani D,Gosselin G,Imbach JL

    更新日期:1991-02-12 00:00:00

  • Allosteric models for multimeric proteins: oxygen-linked effector binding in hemocyanin.

    abstract::In many crustaceans, changing concentrations of several low molecular weight compounds modulates hemocyanin oxygen binding, resulting in lower or higher oxygen affinities of the pigment. The nonphysiological effector caffeine and the physiological modulator urate, the latter accumulating in the hemolymph of the lobste...


    pub_type: 杂志文章


    authors: Menze MA,Hellmann N,Decker H,Grieshaber MK

    更新日期:2005-08-02 00:00:00

  • Enzymatic Synthesis of a Fluorogenic Reporter Substrate and the Development of a High-Throughput Assay for Fucosyltransferase VIII Provide a Toolkit to Probe and Inhibit Core Fucosylation.

    abstract::We report a straightforward enzymatic synthesis of the 4-methylumbelliferyl glycoside of a complex-type oligosaccharide substrate for core fucosylation. We demonstrate the use of this synthetic glycoconjugate in a newly developed enzyme assay to probe the activity and inhibition of fucosyltransferase VIII, which catal...


    pub_type: 杂志文章


    authors: Soroko M,Kwan DH

    更新日期:2020-06-09 00:00:00

  • Cooperative binding of midazolam with testosterone and alpha-naphthoflavone within the CYP3A4 active site: a NMR T1 paramagnetic relaxation study.

    abstract::Recent studies have indicated that CYP3A4 exhibits non-Michaelis-Menten kinetics for numerous substrates. Both homo- and heterotropic activation have been reported, and kinetic models have suggested multiple substrates within the active site. We provide some of the first physicochemical data supporting the hypothesis ...


    pub_type: 杂志文章


    authors: Cameron MD,Wen B,Allen KE,Roberts AG,Schuman JT,Campbell AP,Kunze KL,Nelson SD

    更新日期:2005-11-01 00:00:00

  • Complete amino acid sequence of the 4Fe-4S, thermostable ferredoxin from Clostridium thermoaceticum.

    abstract::The complete amino acid sequence of the 4Fe-4S ferredoxin from the thermophilic bacterium Clostridium thermoaceticum has been determined. The protein is extremely thermostable and is the only known clostridial ferredoxin to contain a single [4Fe-4S] cluster. The sequence totals 63 residues and includes the first trypt...


    pub_type: 杂志文章


    authors: Elliott JI,Yang SS,Ljungdahl LG,Travis J,Reilly CF

    更新日期:1982-07-06 00:00:00

  • DNA-stimulated assembly of oligomeric bacteriophage 434 repressor: evidence for cooperative binding by recruitment.

    abstract::Typical of many transcriptional regulatory proteins, the lambdoid bacteriophage repressors bind cooperatively to multiple sites on DNA. This cooperative binding is essential for establishment and maintenance of phage lysogeny. In the phage, two repressor homodimers, one bound at each of the adjacent operator sites, in...


    pub_type: 杂志文章


    authors: Ciubotaru M,Koudelka GB

    更新日期:2003-04-15 00:00:00

  • Chemistry of gene silencing: the mechanism of NAD+-dependent deacetylation reactions.

    abstract::The Sir2 enzyme family is responsible for a newly classified chemical reaction, NAD(+)-dependent protein deacetylation. New peptide substrates, the reaction mechanism, and the products of the acetyl transfer to NAD(+) are described for SIR2. The final products of SIR2 reactions are the deacetylated peptide and the 2' ...


    pub_type: 杂志文章


    authors: Sauve AA,Celic I,Avalos J,Deng H,Boeke JD,Schramm VL

    更新日期:2001-12-25 00:00:00

  • Conformational adaptation of apolipoprotein A-I to discretely sized phospholipid complexes.

    abstract::The conformational constraints for apoA-I bound to recombinant phospholipid complexes (rHDL) were attained from a combination of chemical cross-linking and mass spectrometry. Molecular distances were then used to refine models of lipid-bound apoA-I on both 80 and 96 A diameter rHDL particles. To obtain molecular const...


    pub_type: 杂志文章


    authors: Bhat S,Sorci-Thomas MG,Tuladhar R,Samuel MP,Thomas MJ

    更新日期:2007-07-03 00:00:00

  • Analysis of the structural core of the human estrogen receptor ligand binding domain by selective proteolysis/mass spectrometric analysis.

    abstract::The structure of the ca. 250 amino acid hormone binding domain of the human estrogen receptor (hER-LBD), expressed in E. coli and purified as a complex with estradiol, has been probed by selective proteolysis, with analysis of the protein fragments both by classical methods (SDS-PAGE and Edman N-terminal sequencing) a...


    pub_type: 杂志文章


    authors: Seielstad DA,Carlson KE,Kushner PJ,Greene GL,Katzenellenbogen JA

    更新日期:1995-10-03 00:00:00

  • Correlation between the inhibition of the acto-heavy meromyosin ATPase and the binding of tropomyosin to F-actin: effects of Mg2+, KCl, troponin I, and troponin C.

    abstract::When stoichiometric amounts of tropomyosin (TM) are bound to F-actin in the presence of 2 mM ATP, the MG2+-activated acto-heavy meromyosin (HMM) ATPase is inhibited by about 60% in 5 mM MgCl2-30 mM KCl. If the concentration of MgCl2 is reduced to 1 mM, the inhibition disappears because TM no longer binds to F-actin. I...


    pub_type: 杂志文章


    authors: Eaton BL,Kominz DR,Eisenberg E

    更新日期:1975-06-17 00:00:00

  • Selenium as an electron acceptor during the catalytic mechanism of thioredoxin reductase.

    abstract::Mammalian thioredoxin reductase (TR) is a pyridine nucleotide disulfide oxidoreductase that uses the rare amino acid selenocysteine (Sec) in place of the more commonly used amino acid cysteine (Cys) in the redox-active tetrapeptide Gly-Cys-Sec-Gly motif to catalyze thiol/disulfide exchange reactions. Sec can accelerat...


    pub_type: 杂志文章


    authors: Lothrop AP,Snider GW,Ruggles EL,Patel AS,Lees WJ,Hondal RJ

    更新日期:2014-02-04 00:00:00