Resonance Raman interrogation of the consequences of heme rotational disorder in myoglobin and its ligated derivatives.


:Resonance Raman spectroscopy is employed to characterize heme site structural changes arising from conformational heterogeneity in deoxyMb and ligated derivatives, i.e., the ferrous CO (MbCO) and ferric cyanide (MbCN) complexes. The spectra for the reversed forms of these derivatives have been extracted from the spectra of reconstituted samples. Dramatic changes in the low-frequency spectra are observed, where newly observed RR modes of the reversed forms are assigned using protohemes that are selectively deuterated at the four methyl groups or at the four methine carbons. Interestingly, while substantial changes in the disposition of the peripheral vinyl and propionate groups can be inferred from the dramatic spectral shifts, the bonds to the internal histidyl imidazole ligand and those of the Fe-CO and Fe-CN fragments are not significantly affected by the heme rotation, as judged by lack of significant shifts in the nu(Fe-N(His)), nu(Fe-C), and nu(C-O) modes. In fact, the apparent lack of an effect on these key vibrational parameters of the Fe-N(His), Fe-CO, and Fe-CN fragments is entirely consistent with previously reported equilibrium and kinetic studies that document virtually identical functional properties for the native and reversed forms.






Rwere F,Mak PJ,Kincaid JR




Has Abstract


2008-12-02 00:00:00












  • Nuclear magnetic resonance studies of carbonic anhydrase catalyzed reversible hydration of acetaldehyde by the saturation transfer method.

    abstract::The catalysis of the chemical exchange between acetaldehyde and its hydrate by bovine carbonic anhydrase B was investigated by the NMR line broadening and saturation transfer techniques. While both chemical exchange and binding to the enzyme had an effect on the line broadening of acetaldehyde and its hydrate, the sat...


    pub_type: 杂志文章


    authors: Cheshnovsky D,Navon G

    更新日期:1980-04-29 00:00:00

  • Trapping of transition metal-nucleotide complexes in myosin subfragment 1 by cross-linking thiols; divalent transition metal probes of the active site.

    abstract::Recent experiments [Wells, J., & Yount, R. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 4966] have shown it is possible to trap MgADP and other nucleotides stably at the active site of myosin by cross-linking two thiol groups. A variety of cross-linking reagents including chelation of the two thiols by cobalt (III) phenan...


    pub_type: 杂志文章


    authors: Dalbey RE,Wells JA,Yount RG

    更新日期:1983-01-18 00:00:00

  • Isotopic probes of the argininosuccinate lyase reaction.

    abstract::The mechanism of the argininosuccinate lyase reaction has been probed by the measurement of the effects of isotopic substitution at the reaction centers. A primary deuterium isotope effect of 1.0 on both V and V/K is obtained with (2S,3R)-argininosuccinate-3-d, while a primary 15N isotope effect on V/K of 0.9964 +/- 0...


    pub_type: 杂志文章


    authors: Kim SC,Raushel FM

    更新日期:1986-08-26 00:00:00

  • Folding of Aplysia limacina apomyoglobin involves an intermediate in common with other evolutionarily distant globins.

    abstract::In the globin family, similarities in the folding mechanism have been found among different mammalian apomyoglobins (apoMb). The best-characterized intermediate of sperm whale apoMb, called I(AGH), is mainly stabilized by nativelike contacts among the A, G, and H helices involving a cluster of hydrophobic residues tha...


    pub_type: 杂志文章


    authors: Musto R,Bigotti MG,Travaglini-Allocatelli C,Brunori M,Cutruzzolà F

    更新日期:2004-01-13 00:00:00

  • Synergistic interactions of multiple mutations on catalysis during the hydroxylation reaction of p-hydroxybenzoate hydroxylase: studies of the Lys297Met, Asn300Asp, and Tyr385Phe mutants reconstituted with 8-Cl-flavin.

    abstract::The oxygen transfer to p-hydroxybenzoate catalyzed by p-hydroxybenzoate hydroxylase (PHBH) has been shown to occur via a C4a-hydroperoxide of the flavin. Two factors are likely to be important in facilitating the transfer of oxygen from the C4a-hydroperoxide to the substrate. (a) The positive electrostatic potential o...


    pub_type: 杂志文章


    authors: Ortiz-Maldonado M,Aeschliman SM,Ballou DP,Massey V

    更新日期:2001-07-31 00:00:00

  • Calcium regulation of calmodulin binding to and dissociation from the myo1c regulatory domain.

    abstract::Myo1c is an unconventional myosin involved in cell signaling and membrane dynamics. Calcium binding to the regulatory-domain-associated calmodulin affects myo1c motor properties, but the kinetic details of this regulation are not fully understood. We performed actin gliding assays, ATPase measurements, fluorescence sp...


    pub_type: 杂志文章


    authors: Manceva S,Lin T,Pham H,Lewis JH,Goldman YE,Ostap EM

    更新日期:2007-10-23 00:00:00

  • Self-association of rabbit muscle phosphofructokinase at pH 7.0: stoichiometry.

    abstract::The self-association of rabbit muscle phosphofructokinase at pH 7.0 was investigated by velocity sedimentation. The process was demonstrated to be in a rapid, dynamic equilibrium. The concentration dependence of the weight-average sedimentation coefficient was monitored within the range of 10-750 microgram/mL. The sed...


    pub_type: 杂志文章


    authors: Hesterberg LK,Lee JC

    更新日期:1981-05-12 00:00:00

  • Stabilization of a protein nanocage through the plugging of a protein-protein interfacial water pocket.

    abstract::The unique structural properties of the ferritin protein cages have provided impetus to focus on the methodical study of these self-assembling nanosystems. Among these proteins, Escherichia coli bacterioferritin (EcBfr), although architecturally very similar to other members of the family, shows structural instability...


    pub_type: 杂志文章


    authors: Ardejani MS,Li NX,Orner BP

    更新日期:2011-05-17 00:00:00

  • Local conformational transition of Hydrogenobacter thermophilus cytochrome c552 relevant to its redox potential.

    abstract::In order to elucidate the molecular mechanisms responsible for the apparent nonlinear behavior of the temperature dependence of the redox potential of Hydrogenobacter thermophilus cytochrome c552 [Takahashi, Y., Sasaki, H., Takayama, S. J., Mikami, S., Kawano, S., Mita, H., Sambongi, Y., and Yamamoto, Y. (2006) Bioche...


    pub_type: 杂志文章


    authors: Takayama SJ,Takahashi YT,Mikami S,Irie K,Kawano S,Yamamoto Y,Hemmi H,Kitahara R,Yokoyama S,Akasaka K

    更新日期:2007-08-14 00:00:00

  • Probing adenine rings and backbone linkages using base specific isotope-edited Raman spectroscopy: application to group II intron ribozyme domain V.

    abstract::Raman difference spectroscopy is used to probe the properties of a 36-nt RNA molecule, "D5", which lies at the heart of the catalytic apparatus in group II introns. For D5 that has all of its adenine residues labeled with (13)C and (15)N and utilizing Raman difference spectroscopy, we identify the conformationally sen...


    pub_type: 杂志文章


    authors: Chen Y,Eldho NV,Dayie TK,Carey PR

    更新日期:2010-04-27 00:00:00

  • Deuterium NMR investigation of backbone dynamics in the synthetic oligonucleotide [d(CGCGAATTCGCG)]2.

    abstract::Backbone dynamics in the [5',5"-2H2]2'-deoxythymidine labeled duplex dodecamer [d-(CGCGAAT*T*CGC)]2 have been investigated by solid-state 2H NMR. Quadrupolar echo line shapes, spin-lattice relaxation, and quadrupolar echo decay times were obtained over hydration levels ranging from W = 0.0 to 25.2 (moles of H2O/mole o...


    pub_type: 杂志文章


    authors: Alam TM,Orban J,Drobny GP

    更新日期:1991-09-24 00:00:00

  • Distinct Differences in Structural States of Conserved Histidines in Two Related Proteins: NMR Studies of the Chemokines CXCL1 and CXCL8 in the Free Form and Macromolecular Complexes.

    abstract::Hydrogen-bonding and ionic interactions play fundamental roles in macromolecular recognition and function. In contrast to lysines and arginines, how histidines mediate these interactions is less well-understood due to the unique properties of its side chain imidazole that include an aromatic ring with two titratable n...


    pub_type: 杂志文章


    authors: Sepuru KM,Rajarathnam K

    更新日期:2018-10-16 00:00:00

  • Lipid bilayer partitioning and stability of camptothecin drugs.

    abstract::The intense intrinsic fluorescence emissions from several clinically relevant camptothecin drugs have been exploited in order to determine (1) the structural basis of drug binding to lipid bilayers, (2) the lipid bilayer stability of each drug's alpha-hydroxylactone moiety, a pharmacophore which is essential for antit...


    pub_type: 杂志文章


    authors: Burke TG,Mishra AK,Wani MC,Wall ME

    更新日期:1993-05-25 00:00:00

  • Importance of phosphatidylethanolamine for association of protein kinase C and other cytoplasmic proteins with membranes.

    abstract::Biological membranes exhibit an asymmetric distribution of phospholipids. Phosphatidylserine (PS) is an acidic phospholipid that is found almost entirely on the interior of the cell where it is important for interaction with many cellular components. A less well understood phenomenon is the asymmetry of the neutral ph...


    pub_type: 杂志文章


    authors: Bazzi MD,Youakim MA,Nelsestuen GL

    更新日期:1992-02-04 00:00:00

  • A fluorescent pentameric thiophene derivative detects in vitro-formed prefibrillar protein aggregates.

    abstract::Protein aggregation is associated with a wide range of diseases, and molecular probes that are able to detect a diversity of misfolded protein assemblies are of great importance. The identification of prefibrillar states preceding the formation of well-defined amyloid fibrils is of particular interest both because of ...


    pub_type: 杂志文章


    authors: Hammarström P,Simon R,Nyström S,Konradsson P,Aslund A,Nilsson KP

    更新日期:2010-08-17 00:00:00

  • Structural effect of the Asp345a insertion in penicillin-binding protein 2 from penicillin-resistant strains of Neisseria gonorrhoeae.

    abstract::A hallmark of penicillin-binding protein 2 (PBP2) from penicillin-resistant strains of Neisseria gonorrhoeae is insertion of an aspartate after position 345. The insertion resides on a loop near the active site and is immediately adjacent to an existing aspartate (Asp346) that forms a functionally important hydrogen b...


    pub_type: 杂志文章


    authors: Fedarovich A,Cook E,Tomberg J,Nicholas RA,Davies C

    更新日期:2014-12-09 00:00:00

  • Tyr51: Key Determinant of the Low Thermostability of the Colwellia psychrerythraea Cold-Shock Protein.

    abstract::Cold-shock proteins (Csps) are expressed at lower-than-optimum temperatures, and they function as RNA chaperones; however, no structural studies on psychrophilic Csps have been reported. Here, we aimed to investigate the structure and dynamics of the Csp of psychrophile Colwellia psychrerythraea 34H, ( Cp-Csp). Althou...


    pub_type: 杂志文章


    authors: Lee Y,Kwak C,Jeong KW,Durai P,Ryu KS,Kim EH,Cheong C,Ahn HC,Kim HJ,Kim Y

    更新日期:2018-07-03 00:00:00

  • Crystal structures of substrate and substrate analog complexes of protocatechuate 3,4-dioxygenase: endogenous Fe3+ ligand displacement in response to substrate binding.

    abstract::Protocatechuate 3,4-dioxygenase (3,4-PCD) utilizes a ferric ion to catalyze the aromatic ring cleavage of 3,4-dihydroxybenzoate (PCA) by incorporation of both atoms of dioxygen to yield beta-carboxy-cis, cis-muconate. The crystal structures of the anaerobic 3,4-PCD.PCA complex, aerobic complexes with two heterocyclic ...


    pub_type: 杂志文章


    authors: Orville AM,Lipscomb JD,Ohlendorf DH

    更新日期:1997-08-19 00:00:00

  • Structure of the ternary complex formed by a chemotaxis receptor signaling domain, the CheA histidine kinase, and the coupling protein CheW as determined by pulsed dipolar ESR spectroscopy.

    abstract::The signaling apparatus that controls bacterial chemotaxis is composed of a core complex containing chemoreceptors, the histidine autokinase CheA, and the coupling protein CheW. Site-specific spin labeling and pulsed dipolar ESR spectroscopy (PDS) have been applied to investigate the structure of a soluble ternary com...


    pub_type: 杂志文章


    authors: Bhatnagar J,Borbat PP,Pollard AM,Bilwes AM,Freed JH,Crane BR

    更新日期:2010-05-11 00:00:00

  • Streptococcus pneumoniae isoprenoid biosynthesis is downregulated by diphosphomevalonate: an antimicrobial target.

    abstract::The toll that Streptococcus pneumoniae exacts on the welfare of humanity is enormous. This organism claims the lives of approximately 3700 people daily, the majority of whom are children below the age of 5, and the situation could worsen due to the increasing incidence of pernicious, multiple-antibiotic-resistant stra...


    pub_type: 杂志文章


    authors: Andreassi JL 2nd,Dabovic K,Leyh TS

    更新日期:2004-12-28 00:00:00

  • Quaternary structure of the HSC70 cochaperone HIP.

    abstract::HSC70 interacting protein (HIP) is an essential cytoplasmic cochaperone involved in the regulation of HSC70 chaperone activity and the maturation of progesterone receptor. To determine the quaternary structure and the gross conformation of the protein in solution, a wide array of biochemical and biophysical techniques...


    pub_type: 杂志文章


    authors: Velten M,Villoutreix BO,Ladjimi MM

    更新日期:2000-01-18 00:00:00

  • Peripheral benzodiazepine receptor antisense knockout increases tumorigenicity of MA-10 Leydig cells in vivo and in vitro.

    abstract::Peripheral benzodiazepine receptors (PBR), first described more than 20 years ago, have been attributed with many putative functions including ones in cellular proliferation and cellular respiration. Hence, it is quite conceivable that deregulation of this receptor could lead to pathology. We and others have reported ...


    pub_type: 杂志文章


    authors: Weisinger G,Kelly-Hershkovitz E,Veenman L,Spanier I,Leschiner S,Gavish M

    更新日期:2004-09-28 00:00:00

  • Design, Synthesis, and Potency of Pyruvate Dehydrogenase Complex E1 Inhibitors against Cyanobacteria.

    abstract::Safe and effective algaecides are needed to control agriculturally and environmentally significant algal species. Four series (6, 10, 17, and 21) of 29 novel 4-aminopyrimidine derivatives were rationally designed and synthesized. A part of 10, 17, and 21 displayed potent inhibition of Escherichia coli pyruvate dehydro...


    pub_type: 杂志文章


    authors: Zhou Y,Feng J,He H,Hou L,Jiang W,Xie D,Feng L,Cai M,Peng H

    更新日期:2017-12-12 00:00:00

  • Effect of protein-protein interaction on light adaptation of bacteriorhodopsin.

    abstract::Triton X-100 solubilized monomers of bacteiorhodopsin (bR) show a decrease in the extent of light adaptation; the red shift and the absorbance increase of the visible absorption band are reduced no less than half the values observed in purple membrane (p.m.) with a corresponding reduction in the isomerization of 13-ci...


    pub_type: 杂志文章


    authors: Casadio R,Stoeckenius W

    更新日期:1980-07-08 00:00:00

  • Efficient repair of 8-oxo-7,8-dihydrodeoxyguanosine in human and hamster xeroderma pigmentosum D cells.

    abstract::The repair of the endogenous lesion 8-oxo-7,8-dihydrodeoxyguanosine (8-oxodG) was investigated in the nucleotide excision repair mutant xeroderma pigmentosum D (XPD), using human normal or transformed XPD fibroblasts and the Chinese hamster XPD cell line UV5. In vivo repair of 8-oxodG induced by hydrogen peroxide trea...


    pub_type: 杂志文章


    authors: Cappelli E,Degan P,Thompson LH,Frosina G

    更新日期:2000-08-29 00:00:00

  • A 106-kDa aminopeptidase is a putative receptor for Bacillus thuringiensis Cry11Ba toxin in the mosquito Anopheles gambiae.

    abstract::Bacillus thuringiensis (Bt) insecticidal toxins bind to receptors on midgut epithelial cells of susceptible insects, and binding triggers biochemical events that lead to insect mortality. Recently, a 100-kDa aminopeptidase N (APN) was isolated from brush border membrane vesicles (BBMV) of Anopheles quadrimaculatus and...


    pub_type: 杂志文章


    authors: Zhang R,Hua G,Andacht TM,Adang MJ

    更新日期:2008-10-28 00:00:00

  • WIN 17317-3, a new high-affinity probe for voltage-gated sodium channels.

    abstract::The iminodihydroquinoline WIN 17317-3 was previously shown to inhibit selectively the voltage-gated potassium channels, K(v)1.3 and K(v)1.4 [Hill, R. J., et al. (1995) Mol. Pharmacol. 48, 98-104; Nguyen, A., et al. (1996) Mol. Pharmacol. 50, 1672-1679]. Since these channels are found in brain, radiolabeled WIN 17317-3...


    pub_type: 杂志文章


    authors: Wanner SG,Glossmann H,Knaus HG,Baker R,Parsons W,Rupprecht KM,Brochu R,Cohen CJ,Schmalhofer W,Smith M,Warren V,Garcia ML,Kaczorowski GJ

    更新日期:1999-08-24 00:00:00

  • Supercoil-accelerated DNA threading intercalation.

    abstract::The effect of DNA supercoiling on a sterically very demanding threading intercalation process is investigated here. We find that the threading rate of a dimeric ruthenium complex into a negatively supercoiled plasmid at low binding density is 2 orders of magnitude higher than into the cleaved linear form. Further satu...


    pub_type: 杂志文章


    authors: Nordell P,Jansson ET,Lincoln P

    更新日期:2009-02-24 00:00:00

  • Heightened Dynamics of the Oxidized Y48H Variant of Human Cytochrome c Increases Its Peroxidatic Activity.

    abstract::Proteins performing multiple biochemical functions are called "moonlighting proteins" or extreme multifunctional (EMF) proteins. Mitochondrial cytochrome c is an EMF protein that binds multiple partner proteins to act as a signaling molecule, transfers electrons in the respiratory chain, and acts as a peroxidase in ap...


    pub_type: 杂志文章


    authors: Deacon OM,Karsisiotis AI,Moreno-Chicano T,Hough MA,Macdonald C,Blumenschein TMA,Wilson MT,Moore GR,Worrall JAR

    更新日期:2017-11-21 00:00:00

  • Using 2-aminopurine fluorescence to detect bacteriophage T4 DNA polymerase-DNA complexes that are important for primer extension and proofreading reactions.

    abstract::The fluorescence of the base analogue 2-aminopurine (2AP) was used to probe bacteriophage T4 DNA polymerase-induced conformational changes in the template strand produced during the nucleotide incorporation and proofreading reactions. 2AP fluorescence in DNA is quenched by 2AP interactions with neighboring bases, but ...


    pub_type: 杂志文章


    authors: Hariharan C,Reha-Krantz LJ

    更新日期:2005-12-06 00:00:00