Abstract：
:The formation of pseudouridine (psi) on U5 small nuclear RNA (U5 snRNA) was studied using an in vitro modification system. Labeled U5 RNA, synthesized in vitro and therefore unmodified, was incubated in reactions containing S100 and/or nuclear extracts (NE) from HeLa cells, and the levels of psi were determined. There are three psi residues found in human U5 RNA, at positions 43, 46, and 53. Incubation of unmodified U5 RNA in reactions containing either S100 or NE supports psi formation at positions 43 and 46, which are found in a loop in the predicted secondary structure of U5 RNA. However, psi formation at position 53, which is found in a stem, is dependent on the presence of NE during the incubation. The order of extract addition does not have a significant effect on the formation of psi at position 53 as long as NE is present. The most efficient psi formation was observed with a combination of S100 and NE which allowed for efficient small nuclear ribonucleoprotein particle (snRNP) assembly and psi formation. When 9S and 20S U5 snRNPs were isolated by velocity sedimentation gradient centrifugation after incubation in the combined extracts, there was little difference in the psi levels at any of the positions for the two distinct particles. Mutations in the U5 RNA sequence do affect psi formation. U5 RNAs that have mutated Sm binding sites or are truncated prior to the Sm binding site have very low levels of psi formation at positions 43 and 46 and no detectable psi formation at position 53. A deletion of five nucleotides from 39 to 43 abolishes psi formation at positions 43 and 46, but the modification of position 53 is unaffected.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Patton JRdoi
10.1021/bi00200a025subject
Has Abstractpub_date
1994-08-30 00:00:00pages
10423-7issue
34eissn
0006-2960issn
1520-4995journal_volume
33pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The human double-stranded RNA- (dsRNA) activated protein kinase (PKR) has a dsRNA-binding domain (dsRBD) that contains two tandem copies of the dsRNA-binding motif (dsRBM). The minimal-length polypeptide required to bind dsRNA contains both dsRBMs, as determined by mobility-shift and filter-binding assays. Mobility-sh...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9607259
更新日期：1996-08-06 00:00:00
abstract：:The allosteric interactions that regulate substrate channeling and catalysis in the tryptophan synthase bienzyme complex from Salmonella typhimurium are triggered by covalent reactions at the beta-site and binding of substrate/product to the alpha-site. The transmission of these allosteric signals between the alpha- a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034291a
更新日期：2003-07-01 00:00:00
abstract：:A quartz-crystal microbalance (QCM) technique was applied to analyze effects of site-directed mutagenesis of a glycosidase (isomalto-dextranase) on the hydrolysis mechanism of the substrate binding (k(on), k(off), and K(d)) and the catalytic process (k(cat)), separately, by using a dextran-immobilized QCM in buffer so...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050079q
更新日期：2005-07-12 00:00:00
abstract：:A homogeneous preparation of transketolase was obtained from spinach leaf; the specific enzyme activity was 9.5 mumolo of glyceraldehyde-3-P formed (mg of protein)-1 min-1, when xylulose-5-P and ribose-5-P were used as the donor and acceptor, respectively, of the ketol residue. Transketolase catalyzed the formation of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00558a015
更新日期：1980-08-19 00:00:00
abstract：:In addition to encoding the tertiary fold and stability, the primary sequence of a protein encodes the folding trajectory and kinetic barriers that determine the speed of folding. How these kinetic barriers are encoded is not well understood. Here, we use evolutionary sequence variation in the α-lytic protease (αLP) p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00720
更新日期：2021-01-26 00:00:00
abstract：:Islet-activating protein (IAP), pertussis toxin, is a hexameric protein composed of an A protomer and a B oligomer, the residual pentamer having such a subunit assembly that two different dimers, dimer 1 and dimer 2, are connected with each other by means of the smallest C subunit. Incubation of IAP with formaldehyde ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00354a025
更新日期：1986-03-25 00:00:00
abstract：:Lysophosphatidic acid (1-acyl-sn-glycero-3-phosphate or LPA) is a phospholipid mediator displaying numerous and widespread biological activities and thought to act via G-protein-coupled receptors. Here we have studied the effects on human platelets of a number of LPA analogues, including two enantiomers of both N-palm...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9816756
更新日期：1999-06-29 00:00:00
abstract：:The binding of ethidium bromide to the self-complementary deoxytetranucleotides containing guanine and cytosine bases has been studied by circular dichroism, visible absorption, and fluorescence spectroscopies. The circular dichroism spectrum of each of the four deoxytetranucleotides was measured and compared to the s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00616a002
更新日期：1978-11-14 00:00:00
abstract：:The effect of long-term storage on the electronic absorption spectrum and the kinetic properties of cytochrome c peroxidase has been investigated. No detectable differences were observed between freshly isolated enzyme and enzyme stored below -20 degrees C, in the crystalline state, for up to 41 months. The electronic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00470a004
更新日期：1990-05-08 00:00:00
abstract：:We have examined in detail the kinetics of binding of the serpin alpha 2-antiplasmin to the serine proteases alpha-chymotrypsin and plasmin. These represent model systems for serpin binding. We find, in contrast to earlier published results with alpha 2-antiplasmin and plasmin, that binding is reversible, and slow bin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00218a014
更新日期：1991-01-29 00:00:00
abstract：:Recognition of and binding to cholesterol on the host cell membrane is an initial step in the mechanism of numerous pathogens, including viruses, bacteria, and bacterial toxins; however, a viable method of inhibiting this interaction has not yet been uncovered. Here, we describe the mechanism by which a cholesterol re...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00430
更新日期：2016-08-30 00:00:00
abstract：:The structural features of a chemically modified DNA template strand may promote error-prone DNA synthesis during replication. The resulting higher incidence of mutations, in turn, can eventually lead to tumor initiation. Structural insights into this process can be monitored by studying chemically modified base adduc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00046a043
更新日期：1995-11-21 00:00:00
abstract：:The conformations of melittin, an amphipathic polypeptide consisting of 26 amino acid residues, and its hydrophobic (residues 1--19) and hydrophilic (residues 20--26) fragments were examined in various solvent systems, including H2O, 2H2O, 2-chloroethanol, and 1,2-dimyristoylphosphatidylcholine (DMPC) multilayers, by ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00539a006
更新日期：1982-05-11 00:00:00
abstract：:Tau is a neuronal microtubule-associated protein that plays a central role in many cellular processes, both physiological and pathological, such as axons stabilization and Alzheimer's disease. Despite extensive studies, very little is known about the detailed molecular basis of tau binding to microtubules. We used the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0493160
更新日期：2004-08-17 00:00:00
abstract：:The primary electron acceptor complex of photosystem II, QAFe2+, can bind a number of small molecules at the iron site, including cyanide [Koulougliotis, D., Kostopoulos, T., Petrouleas, V., & Diner, B. A. (1993) Biochim. Biophys. Acta 1141, 275-282)]. In the presence of NaCN (30-300 mM) at pH 6.5, the reduced state, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00199a014
更新日期：1994-08-23 00:00:00
abstract：:The folding of the enzyme indole-3-glycerol-phosphate synthase (IGPS), a member of the (alpha/beta)8 fold family, has been studied. At least two folding intermediates have been detected using spectroscopic and activity measurements in combination with gel filtration chromatography. These two intermediates are produced...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963133z
更新日期：1997-05-06 00:00:00
abstract：:We have examined the competitive binding of oxygen and carbon monoxide to the multisubunit hemocyanin of the tarantula Eurypelma californicum. Employment of high-precision thin-layer methods has enabled detailed characterization of the pure oxygen and pure carbon monoxide binding curves, as well as binding curves perf...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00418a036
更新日期：1988-09-06 00:00:00
abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins as well as the in-vitro reconstitution of the first steps of this biosynthesis. We now report experimental evidence supporting the propos...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200892a
更新日期：2011-08-23 00:00:00
abstract：:Biotin synthase, a member of the "radical SAM" family, catalyzes the final step of the biotin biosynthetic pathway, namely, the insertion of a sulfur atom into dethiobiotin (DTB). The active form of the enzyme contains two iron-sulfur clusters, a [4Fe-4S](2+) cluster liganded by Cys-53, Cys-57, and Cys-60 and the S-ad...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060662m
更新日期：2006-10-10 00:00:00
abstract：:Postsynaptic polypeptide neurotoxins isolated from the venoms of elapid and hydrophid snakes exhibit the ability to bind selectively to and inhibit different types of receptors that function in nerve signal transmission. On the basis of their amino acid sequences and three-dimensional structures, these neurotoxins are...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00179a011
更新日期：1994-04-05 00:00:00
abstract：:In type 2 diabetics, the hormone amylin misfolds into amyloid plaques implicated in the destruction of the pancreatic β-cells that make insulin and amylin. The aggregative misfolding of amylin is pH-dependent, and exposure of the hormone to acidic and basic environments could be physiologically important. Amylin has t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401164k
更新日期：2014-01-21 00:00:00
abstract：:Multidimensional heteronuclear NMR techniques were applied to study the phosphotransfer domain, residues 1-134, of the histidine kinase CheA, from Escherichia coli, which contains the site of autophosphorylation, His48. Assignments of the backbone amide groups and side chain protons are nearly complete. Our studies sh...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00042a018
更新日期：1995-10-24 00:00:00
abstract：:The rapid expansion of the amount of genomic and structural data has provided many examples of enzymes with evolutionarily related active sites that catalyze different reactions. Functional comparisons of these active sites can provide insight into the origins of the enormous catalytic proficiency of enzymes and the e...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060847t
更新日期：2006-08-15 00:00:00
abstract：:Previous studies have shown that the short-motif electroplax Na channel is phosphorylated in vitro by cyclic AMP-dependent protein kinase (PKA) at serines 6 or 7 and 1776 and threonine 17 (Emerick & Agnew, 1989). We here show that phosphatase treatment of solubilized, purified Na channels enhanced subsequent PKA label...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00087a023
更新日期：1993-09-14 00:00:00
abstract：:The formation and role of arachidonic acid (AA) and its metabolites during gonadotropin releasing hormone- (GnRH-) induced gonadotropin secretion were investigated in primary cultures of rat pituitary cells. Prelabeled cells ([3H]AA) responded to GnRH challenge with increased formation (about 2-fold) of the leukotrien...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00139a004
更新日期：1992-06-23 00:00:00
abstract：:The molecular chaperones Hsp90 and Hsp70 are highly regulated by various cochaperones that participate in the activation of steroid receptors. Here we study Tpr2 (also called DjC7), a TPR domain-containing type III J protein implicated in steroid receptor chaperoning. We propose that Tpr2 plays a role in the Hsp90-dep...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800770g
更新日期：2008-08-05 00:00:00
abstract：:A double mutant of the single-domain protein barstar having a single tryptophan (W53) was made by mutating the remaining two tryptophans (W38 and W44) into phenylalanines. W53 is buried in the core of barstar. Time-resolved fluorescence of the mutant barstar (W38FW44F) showed that W53 has a single fluorescence lifetim...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9603478
更新日期：1996-07-16 00:00:00
abstract：:The incorporation of 7-dehydrocholesterol into synthetic phospholipid bilayers altered the distribution of products after photolysis. In liposomes, the relative amounts of 7-dehydrocholesterol and lumisterol were elevated, and tachysterol was reduced from the levels observed in hexane solution. Z to E isomerization of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00334a039
更新日期：1985-06-18 00:00:00
abstract：:The vast majority of characterized oxygenases use bound cofactors to activate molecular oxygen to carry out oxidation chemistry. Here, we show that an enzyme of unknown activity, RhCC from Rhodococcus jostii RHA1, functions as an oxygenase, using 4-hydroxyphenylenolpyruvate as a substrate. This unique and complex reac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501200j
更新日期：2015-02-10 00:00:00
abstract：:Site-directed mutants of human carbonic anhydrase III were used to examine the role of Thr-199 and its interaction with Phe-198 in the catalyzed hydration of CO2. Threonine-199 is a hydrogen bond acceptor for the zinc-bound water, and Phe-198 forms part of the hydrophobic side of the active-site cavity of carbonic anh...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00082a004
更新日期：1993-08-10 00:00:00
