Role of the cochaperone Tpr2 in Hsp90 chaperoning.

abstract：:Hypoxic respiratory diseases or hypoxia exposures are frequently accompanied by glucose intolerance and impaired nitric oxide (NO) availability. However, the molecular mechanism responsible for impaired NO production and insulin resistance (IR) during hypoxia remains obscure. In this study, we investigated the possibl...

journal_title：Biochemistry

authors： Singh B,Biswas I,Garg I,Sugadev R,Singh AK,Dey S,Khan GA

更新日期：2014-01-14 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is a dimeric protein which induces formation of new blood vessels (angiogenesis) through binding to VEGF-receptor-2 tyrosine kinase (VEGFR2 TK) or KDR (kinase insert domain-containing receptor) on the surface of endothelial cells. Angiogenesis has been shown to be essential fo...

journal_title：Biochemistry

authors： Parast CV,Mroczkowski B,Pinko C,Misialek S,Khambatta G,Appelt K

更新日期：1998-11-24 00:00:00

abstract：:Topoisomerase II mediated double-strand breaks produced by anthracycline analogues were studied in SV40 DNA. The compounds included doxorubicin, daunorubicin, two doxorubicin stereoisomers (4'-epimer and beta-anomer), and five chromophore-modified derivatives, with a wide range of cytotoxic activity and DNA binding af...

journal_title：Biochemistry

authors： Capranico G,Zunino F,Kohn KW,Pommier Y

更新日期：1990-01-16 00:00:00

abstract：:The first β-lactone synthetase enzyme is reported, creating an unexpected link between the biosynthesis of olefinic hydrocarbons and highly functionalized natural products. The enzyme OleC, involved in the microbial biosynthesis of long-chain olefinic hydrocarbons, reacts with syn- and anti-β-hydroxy acid substrates t...

journal_title：Biochemistry

authors： Christenson JK,Richman JE,Jensen MR,Neufeld JY,Wilmot CM,Wackett LP

更新日期：2017-01-17 00:00:00

abstract：:After borohydride reduction, carboxymethylation, and tryptic digestion of the holoenzyme of pig heart alanine aminotransferase, a single icosapeptide containing the N6-(phosphopyridoxyl)lysine residue was isolated by a combination of gel filtration and ion-exchange chromatogrpahy. Its primary structure was determined ...

journal_title：Biochemistry

authors： Tanase S,Kojima H,Morino Y

更新日期：1979-07-10 00:00:00

abstract：:The vast majority of characterized oxygenases use bound cofactors to activate molecular oxygen to carry out oxidation chemistry. Here, we show that an enzyme of unknown activity, RhCC from Rhodococcus jostii RHA1, functions as an oxygenase, using 4-hydroxyphenylenolpyruvate as a substrate. This unique and complex reac...

journal_title：Biochemistry

authors： Baas BJ,Poddar H,Geertsema EM,Rozeboom HJ,de Vries MP,Permentier HP,Thunnissen AM,Poelarends GJ

更新日期：2015-02-10 00:00:00

abstract：:We identified amino acid residues important for activity of sod2, the Na+/H+ antiporter of Schizosaccharomyces pombe. We mutated all eight His residues of sod2 into Arg. Only His367-->Arg affected function and resulted in complete inability of sod2 to allow growth of S. pombe in LiCl-containing medium. Mutant S. pombe...

journal_title：Biochemistry

authors： Dibrov P,Young PG,Fliegel L

更新日期：1998-06-09 00:00:00

abstract：:UvrA is the ATPase subunit of the DNA repair enzyme (A)BC excinuclease. The amino acid sequence of this protein has revealed, in addition to two zinc fingers, three pairs of nucleotide binding motifs each consisting of a Walker A and B sequence. We have conducted site-specific mutagenesis, ATPase kinetic analyses, and...

journal_title：Biochemistry

authors： Myles GM,Hearst JE,Sancar A

更新日期：1991-04-23 00:00:00

abstract：:RNA competent in directing protein synthesis is sequestered in unfertilized sea urchin eggs as translationally quiescent, nonpolyribosomal, messenger ribonucleoprotein complexes (mRNPs). Following fertilization, these mRNPs are derepressed and actively translated, presumably due to changes in the mRNA-associated prote...

journal_title：Biochemistry

authors： Moon RT,Moe KD,Hille MB

更新日期：1980-06-10 00:00:00

abstract：:Both activities of the aspartokinase--homoserine I (AK-HSD) of Escherichia coli are inhibited by threonine. Careful threonine binding studies have now been done which have allowed us to distinguish the various effects of threonine on the enzyme. The ultrafiltration technique of H. Paulus ((1969) Anal. Biochem. 32, 101...

journal_title：Biochemistry

authors： Bearer CF,Neet KE

更新日期：1978-08-22 00:00:00

abstract：:Circular dichroism (CD) spectra at variable temperatures have been recorded for human cytosolic sialidase NEU2 in buffered water solutions and in the presence of divalent cations. The results show the prevalence of β-strands together with a considerable amount of α-helical structure, while in the solid state, from bot...

journal_title：Biochemistry

authors： Monti E,Benaglia G,Mozzi A,Fusi P,Longhi G,Gangemi F,Castiglioni E,Woody RW,Fornili SL,Abbate S

更新日期：2014-08-19 00:00:00

abstract：:By use of six highly purified exoglycosidases with well-defined specificity, the oligosaccharide units of human plasma beta 2-glycoprotein I (beta 2I) were modified by sequential enzymatic degradation. The released monosaccharides (NeuAc, Gal, GlcNAc, and Man) were quantified, and the carbohydrate compositions of the ...

journal_title：Biochemistry

authors： Walsh MT,Watzlawick H,Putnam FW,Schmid K,Brossmer R

更新日期：1990-07-03 00:00:00

abstract：:Phospholipase A(2) (PLA(2)) enzymes become activated by binding to biological membranes and hydrolyze phospholipids to free fatty acids and lyso-phospholipids, the precursors of inflammatory mediators. To understand the functional significance of amino acid residues at key positions, we have studied the effects of the...

journal_title：Biochemistry

authors： Nemec KN,Pande AH,Qin S,Bieber Urbauer RJ,Tan S,Moe D,Tatulian SA

更新日期：2006-10-17 00:00:00

abstract：:Calmodulin-dependent protein phosphatase, one of the major calmodulin-binding proteins in bovine brain, dephosphorylates casein with a specific activity of 15 nmol mg-1 min-1 at 30 degrees C. The stimulation of phosphatase activity by calmodulin is reversed by ethylene glycol bis (beta-aminoethyl ether)-N,N,N',N'-tetr...

journal_title：Biochemistry

authors： Tallant EA,Cheung WY

更新日期：1983-07-19 00:00:00

abstract：:The structure of the specific phosphate binding loop (P-loop) of bovine protein tyrosine phosphatase (BPTP) is very similar to that present in high M(r) PTPases. Site-directed mutagenesis was used to explore the role of several conserved residues involved in forming the P-loop of BPTP. Thus, Ser-19 and Ser-43 were ind...

journal_title：Biochemistry

authors： Evans B,Tishmack PA,Pokalsky C,Zhang M,Van Etten RL

更新日期：1996-10-22 00:00:00

abstract：:Mismatches in DNA occur naturally during replication and as a result of endogenous DNA damaging agents, but the mismatch repair (MMR) pathway acts to correct mismatches before subsequent rounds of replication. Rhodium metalloinsertors bind to DNA mismatches with high affinity and specificity and represent a promising ...

journal_title：Biochemistry

authors： Ernst RJ,Komor AC,Barton JK

更新日期：2011-12-20 00:00:00

abstract：:The interaction between cytochrome c oxidase complex and adenosine triphosphate synthase (F1F0) complex in the purified, dispersed state and embedded in phospholipid vesicles was studied by differential scanning calorimetry and by spin-label electron paramagnetic resonance. The detergent-dispersed cytochrome oxidase a...

journal_title：Biochemistry

authors： Qiu ZH,Yu L,Yu CA

更新日期：1992-03-31 00:00:00

abstract：:The transcriptional activity of the hexafluorinated derivative of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], 26,26,26,27,27,27-hexafluoro-1,25-dihydroxyvitamin D3 [F6-1,25-(OH)2D3], was examined in cultured cells by a transient expression assay (CAT assay) using expression vectors for the rat nuclear vitamin D3 receptor...

journal_title：Biochemistry

authors： Sasaki H,Harada H,Handa Y,Morino H,Suzawa M,Shimpo E,Katsumata T,Masuhiro Y,Matsuda K,Ebihara K

更新日期：1995-01-10 00:00:00

abstract：:The relaxin-like factor (RLF) also named insulin-like 3 (INSL3) consists of two polypeptide chains linked by two interchain and one intrachain disulfide bond. RLF binds to its receptor (LGR8 also named RXFP2) through the B chain and initiates transmembrane communication by activating the adenylate cyclase through the ...

journal_title：Biochemistry

authors： Büllesbach EE,Schwabe C

更新日期：2012-05-22 00:00:00

abstract：:F(1)-ATPase mutations in Escherichia coli that changed the strength of hydrogen bonds between the alpha and beta subunits in a location that links the catalytic site to the interface between the beta catch loop and the gamma subunit were examined. Loss of the ability to form the hydrogen bonds involving alphaS337, bet...

journal_title：Biochemistry

authors： Boltz KW,Frasch WD

更新日期：2006-09-19 00:00:00

abstract：:Factors determining conformations of imidazole axially coordinated to heme in heme proteins were investigated by analyzing 693 hemes in 432 different crystal structures of heme proteins from the Protein Data Bank (PDB), where at least one histidine is ligated to heme. The results from a search of the PDB for protein s...

journal_title：Biochemistry

authors： Zarić SD,Popović DM,Knapp EW

更新日期：2001-07-03 00:00:00

abstract：:The solution conformation is presented for representatives of each of the major classes of asparaginyl oligosaccharides. In this report the conformation of alpha(1-3)-, alpha(1-2)-, beta(1-2)-, and beta(1-4)-linked units is described. The conformational properties of these glycopeptides were determined by high-resolut...

journal_title：Biochemistry

authors： Brisson JR,Carver JP

更新日期：1983-07-19 00:00:00

abstract：:The ubiquitous cyclophilins belong to peptidyl-prolyl cis/trans isomerases (PPIases; EC 5.2.1.8). They are able to catalyze the cis/trans isomerization about peptidyl-prolyl amide bonds. The mode of action of human cytosolic cyclophilin (Cyp18cy) has been studied on substrate analog tetrapeptide-4-nitroanilides contai...

journal_title：Biochemistry

authors： Schutkowski M,Wöllner S,Fischer G

更新日期：1995-10-10 00:00:00

abstract：:In many crustaceans, changing concentrations of several low molecular weight compounds modulates hemocyanin oxygen binding, resulting in lower or higher oxygen affinities of the pigment. The nonphysiological effector caffeine and the physiological modulator urate, the latter accumulating in the hemolymph of the lobste...

journal_title：Biochemistry

authors： Menze MA,Hellmann N,Decker H,Grieshaber MK

更新日期：2005-08-02 00:00:00

abstract：:A three-component soluble methane monooxygenase (sMMO) enzyme system catalyzes the hydroxylation of methane to methanol at a carboxylate-bridged diiron center housed in the alpha-subunit of the hydroxylase (MMOH). Catalysis is facilitated by the presence of a regulatory protein (MMOB) and inhibited by MMOD, a protein ...

journal_title：Biochemistry

authors： Sazinsky MH,Merkx M,Cadieux E,Tang S,Lippard SJ

更新日期：2004-12-28 00:00:00

abstract：:The maltodextrin binding protein from Escherichia coli serves as the initial receptor for both the active transport of and chemotaxis toward a range of linear maltose sugars. The X-ray structures of both the maltose-bound and sugar-free forms of the protein have been previously described [Spurlino, J. C., Lu, G.-Y., &...

journal_title：Biochemistry

authors： Sharff AJ,Rodseth LE,Quiocho FA

更新日期：1993-10-12 00:00:00

abstract：:Previous crystallographic studies in this laboratory demonstrated that immunoglobulin light chains with the same amino acid sequence can have at least two and probably three or more conformations, depending on whether the second member of an interacting pair is a light or heavy chain. If a heavy chain is not available...

journal_title：Biochemistry

authors： Firca JR,Ely KR,Kremser P,Westholm FA,Dorrington KJ,Edmundson AB

更新日期：1978-01-10 00:00:00

abstract：:We have used a fluorescent analogue of ATP, mantATP [2'(3')-O-(N-methylanthraniloyl)-adenosine 5'-triphosphate; Hiratsuka T. (1983) Biochim. Biophys. Acta 742, 496-508], and made a detailed kinetic study of the interaction of mantATP and mantADP with S1 and acto-S1. We have shown that these analogues behave like ATP a...

journal_title：Biochemistry

authors： Woodward SK,Eccleston JF,Geeves MA

更新日期：1991-01-15 00:00:00

abstract：:The self-association properties of recombinant DNA derived human relaxin, and porcine relaxin isolated from porcine ovaries, have been studied by sedimentation equilibrium analytical ultracentrifugation and circular dichroism (CD). The human relaxin ultracentrifuge data were adequately defined by a monomer-dimer self-...

journal_title：Biochemistry

authors： Shire SJ,Holladay LA,Rinderknecht E

更新日期：1991-08-06 00:00:00

abstract：:Furamidine and related diamidines represent a promising series of drugs active against widespread parasites, in particular the Pneumocystic carinii pathogen. In this series, the phenylfuranbenzimidazole diamidine derivative DB293 was recently identified as the first unfused aromatic dication capable of forming stacked...

journal_title：Biochemistry

authors： Bailly C,Tardy C,Wang L,Armitage B,Hopkins K,Kumar A,Schuster GB,Boykin DW,Wilson WD

更新日期：2001-08-21 00:00:00