Abstract：
:The activity of phospholipase A2 from snake venom to hydrolyze bilayers of phosphatidylcholines is greatly enhanced by the presence of the hydrolysis products, lysolecithin and fatty acid, in the bilayer. The fluorescence of several probes of membrane structure was used to monitor changes in bilayer physical properties during vesicle hydrolysis. These changes were compared to emission spectra and fluorescence polarization results occurring upon direct addition of lysolecithin and/or fatty acid to the bilayer. The excimer to monomer ratio of 1,3-bis(1-pyrene)propane was insensitive to vesicle hydrolysis, suggesting that changes in the order of the phospholipid chains were not relevant to the effect of the hydrolysis products on phospholipase activity. The fluorescence of 6-propionyl-2-(dimethylamino)-naphthalene (Prodan) suggested that the polarity of the bilayer in the region of the phospholipid head groups increases as the hydrolysis products accumulate in the bilayer. The fluorescence of 6-dodecanoyl-2-(dimethylamino)naphthalene (Laurdan) confirmed that such effects were restricted to the bilayer surface. Furthermore, the lysolecithin appeared to be the product most responsible for these changes. These results suggested that lysolecithin increases the activity of phospholipase A2 during vesicle hydrolysis by disrupting the bilayer surface, making the phospholipid molecules more accessible to the enzyme active site.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Sheffield MJ,Baker BL,Li D,Owen NL,Baker ML,Bell JDdoi
10.1021/bi00024a003subject
Has Abstractpub_date
1995-06-20 00:00:00pages
7796-806issue
24eissn
0006-2960issn
1520-4995journal_volume
34pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The effects of nearest neighbor interactions between a nucleotide base at the primer 3'-terminus and an incoming deoxyribonucleoside triphosphate on DNA polymerase catalyzed insertion were examined. Kinetics of inserting the fluorescent nucleotide analog 2-aminopurine deoxyribonucleotide (dAPMP) and dAMP opposite a te...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00092a039
更新日期：1993-10-19 00:00:00
abstract：:Protein kinase C and two other proteins with molecular masses of 64 and 32 kDa, purified from bovine brain, constitute a type of protein that binds a large number of calcium ions in a phospholipid-dependent manner. This study suggested that these proteins also induced extensive clustering of acidic phospholipids in th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00246a013
更新日期：1991-08-13 00:00:00
abstract：:The soluble domains of the b and b' subunits of the ATP synthase of the cyanobacterium Synechocystis PCC 6803 were expressed with His tags attached to their N-termini. Following purification, the polypeptides were characterized by chemical cross-linking, analytical ultracentrifugation, and circular dichroism spectrosc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001821j
更新日期：2001-01-09 00:00:00
abstract：:Ultraviolet absorption (UV) and circular dichroism (CD) spectra of wheat germ 5S RNA, when compared to tRNAPhe, indicate a largely base-paired and base-stacked helical structure, containing up to 36 base pairs. Fourier-transform infrared (FT-IR) spectra of tRNAPhe and wheat germ ribosomal 5S RNA have been acquired at ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00311a012
更新日期：1984-07-31 00:00:00
abstract：:cAMP binding of the androgen receptor (AR) from murine skeletal muscle was studied. Testosterone affinity chromatography yielded androgen receptor with about 4000-fold purification. Determination of the cAMP binding in the affinity eluate, by adsorption of protein-cAMP complexes to cellulose ester filters or removal o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00423a013
更新日期：1988-11-15 00:00:00
abstract：:Subunit a of the Escherichia coli ATP synthase is thought to control access of protons to the ring of c subunits during proton-driven ATP synthesis. In this study, the surface exposure of subunit a in the periplasm has been examined using 3-N-maleimidyl-propionyl biocytin labeling in cells permeabilized by polymyxin B...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026649t
更新日期：2003-01-21 00:00:00
abstract：:The structural domains of human apolipoprotein(a) [apo(a)] and its interaction with apolipoprotein B-100 (apo B-100) in the lipoprotein(a) [Lp(a)] particle were investigated by limited proteolysis with thermolysin and cathepsin D. We characterized the proteolytic products by sodium dodecyl sulfate-polyacrylamide gradi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00177a026
更新日期：1994-03-22 00:00:00
abstract：:A newly reported strain of Vibrio cholerae, known as strain O139 Bengal, is the first instance of an encapsulated strain that has caused epidemic cholera. The O-antigenic capsule is the critical antigen for protective immunity. Since mapping of the antigenic epitopes will assist in the development of a protein conjuga...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9910272
更新日期：1999-09-14 00:00:00
abstract：:Our earlier NMR study showed that a two-disulfide variant of hen lysozyme containing intra-alpha-domain disulfide bridges, C6-C127 and C30-C115, is partially folded, with the alpha domain tightly folded to the nativelike conformation and the beta domain flexible or unfolded. With a view that the formation of a third d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049967w
更新日期：2004-06-01 00:00:00
abstract：:Thrombin-activatable fibrinolysis inhibitor (TAFI) circulates as an inactive proenzyme of a carboxypeptidase B-like enzyme (TAFIa). It functions by removing C-terminal lysine residues from partially degraded fibrin that are important in tissue-type plasminogen activator mediated plasmin formation. TAFI was classified ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0115683
更新日期：2002-01-29 00:00:00
abstract：:We have synthesized a series of N-propylamino-substituted benzazepinones (NPSBs) as specific probes for the benzothiazepinone (BTZ) binding domain of muscle L-type calcium channels (LTCCs). NPSBs were identified which possess high affinity for the channel after purification. We synthesized a fluorescent NPSB, DMBODIPY...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00010a039
更新日期：1995-03-14 00:00:00
abstract：:Human genomic clones that span the entire protein S expressed gene (PS alpha) and the 3' two-thirds of the protein S pseudogene (PS beta) have been isolated and characterized. The PS alpha gene is greater than 80 kilobases in length and contains 14 introns and 15 exons, as well as 6 repetitive "Alu" sequences. Exons I...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00486a010
更新日期：1990-08-28 00:00:00
abstract：:Although the terms "excessive reactive oxygen species (ROS)" and "oxidative stress" are widely used, the implications of oxidative stress are often misunderstood. ROS are not a single species but a variety of compounds, each with unique biochemical properties and abilities to react with biomolecules. ROS cause activat...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.6b00703
更新日期：2016-09-27 00:00:00
abstract：:The relationship between GTP hydrolysis and microtubule assembly has been investigated by using a rapid filtration method. Microtubules assembled from phosphocellulose-purified tubulin, double-labeled with [gamma-32P]- and [3H]GTP, were trapped and washed free of unbound nucleotide on glass fiber filters. The transien...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00479a022
更新日期：1990-07-10 00:00:00
abstract：:The EP3 subtype of prostaglandin E2 receptor transduces diverse physiological responses in mammalian tissues through signaling pathways coupled to heterotrimeric G proteins. Distinct cDNA clones encoding five isoforms of the EP3 receptor were isolated from a human uterus cDNA library. The human EP3 receptor isoforms d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00252a016
更新日期：1994-12-06 00:00:00
abstract：:Hydrogen-bonding and ionic interactions play fundamental roles in macromolecular recognition and function. In contrast to lysines and arginines, how histidines mediate these interactions is less well-understood due to the unique properties of its side chain imidazole that include an aromatic ring with two titratable n...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00756
更新日期：2018-10-16 00:00:00
abstract：:Phototropin (phot) is a blue-light sensor protein that elicits several photo responses in plants. Phototropin has two flavin mononucleotide (FMN)-binding domains, LOV1 and LOV2, in its N-terminal half. The C-terminal half is a blue-light-regulated Ser/Thr kinase. Various functional studies have reported that only LOV2...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701851v
更新日期：2008-01-22 00:00:00
abstract：:Six active site mutants of Escherichia coli phosphofructokinase have been constructed and characterized using steady-state kinetics. All but one of the mutants (ES222) have significantly lower maximal activity, implicating these residues in the catalytic process. Replacement of Asp127, the key catalytic residue in the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00153a017
更新日期：1992-09-29 00:00:00
abstract：:The steroidogenic acute regulatory protein (StAR) facilitates the movement of cholesterol from the outer to inner mitochondrial membrane in adrenal and gonadal cells, fostering steroid biosynthesis. MLN64 is a 445-amino acid protein of unknown function. When 218 amino-terminal residues of MLN-64 are deleted, the resul...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000911l
更新日期：2000-09-26 00:00:00
abstract：:In invertebrate photoreceptors, illuminated rhodopsin activates multiple G proteins, which are assumed to initiate multiple phototransduction cascades. In this paper, we focused on one of the phototransduction cascades, which utilizes rhodopsin, a Gq-like G protein, and phospholipase C (PLC). A Gq-like G protein from ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961360v
更新日期：1996-12-10 00:00:00
abstract：:The technique of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) has been applied to a mesophilic (E. coli) dihydrofolate reductase under conditions that allow direct comparison to a thermophilic (B. stearothermophilus) ortholog, Ec-DHFR and Bs-DHFR, respectively. The analysis of hydrogen-deuterium e...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200640s
更新日期：2011-09-27 00:00:00
abstract：:Starting from a standard unfolded state (3.0 M guanidine hydrochloride, pH 7.2), the kinetics of refolding of iso-2-cytochrome c have been investigated as a function of final pH between pH 3 and pH 10. Absorbance in the ultraviolet and visible spectral regions and tryptophan fluorescence are used to monitor folding. O...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00419a020
更新日期：1988-09-20 00:00:00
abstract：:Four new in vivo metabolites of vitamin D3 were isolated from the blood plasma of chicks given large doses of vitamin D3. The metabolites were isolated by methanol-chloroform extraction and a series of chromatographic procedures. By use of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00529a010
更新日期：1981-12-22 00:00:00
abstract：:Cytochrome bc(1), a key enzyme of biological energy conversion, generates or uses a proton motive force through the Q cycle that operates within the two chains of cofactors that embed two catalytic quinone oxidation/reduction sites, the Q(o) site and the Q(i) site. The Q(o) site relies on the joint action of two cofac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801207f
更新日期：2008-11-25 00:00:00
abstract：:Phosphotriesterase homology protein (PHP) is a member of a recently discovered family of proteins related to phosphotriesterase, a hydrolytic, bacterial enzyme with an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates, which are common constituents of chemical warfare agent...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971707+
更新日期：1998-04-14 00:00:00
abstract：:Hydroxyl radical induced formation of a DNA-protein cross-link involving thymine and tyrosine in nucleohistone is described. Hydroxyl radicals were generated in N2O-saturated aqueous solution by ionizing radiation. Samples of nucleohistone were hydrolyzed with HCl and trimethylsilylated. Analysis of irradiated samples...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00434a071
更新日期：1989-04-18 00:00:00
abstract：:Specific intra- and intermolecular quadruplex conformations of model G-DNA oligonucleotides have been identified from their translational and rotational diffusion coefficients in aqueous solution. The transport properties were determined by polarized and depolarized dynamic light scattering. A comparison with hydrodyn...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990750p
更新日期：1999-09-21 00:00:00
abstract：:When Pseudomonas putida ATCC 39167 and plant-deleterious Pseudomonas fluorescens A225 were grown in an iron-deficient culture medium, they each produced two different novel yellow-green fluorescent pseudobactins: P39167-I, II and PA225-I, II. Pseudobactin P39167-I has a molecular formula of C46H65O23N13 and is monoani...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962670g
更新日期：1997-04-08 00:00:00
abstract：:Hydrogenase from Chromatium vinosum contains 1 Ni, 11-12 Fe, and ca. 9 sulfides. EPR and Mössbauer studies of the enzyme prepared in four different oxidation states show that the enzyme contains two Fe4S4 and one Fe3S4 cluster. In the oxidized (2+) state, the Mössbauer parameters of the two Fe4S4 clusters are typical ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00182a029
更新日期：1994-04-26 00:00:00
abstract：:The specificity of trypsin for Arg- and Lys-containing substrates depends upon the presence of Asp189 at the base of the primary binding pocket. The crystal structure of anionic rat trypsin D189S complexed with BPTI reveals that removal of the aspartate side chain permits the binding of a well-ordered acetate ion in a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00177a016
更新日期：1994-03-22 00:00:00
