Comparative hydrogen-deuterium exchange for a mesophilic vs thermophilic dihydrofolate reductase at 25 °C: identification of a single active site region with enhanced flexibility in the mesophilic protein.

abstract：:Attempts to alter the guanine specificity of ribonuclease T1 (RNase T1) by rational or random mutagenesis have failed so far. The RNase T1 variant RV (Lys41Glu, Tyr42Phe, Asn43Arg, Tyr45Trp, and Glu46Asn) designed by combination of a random and a rational mutagenesis approach, however, exhibits a stronger preference t...

journal_title：Biochemistry

authors： Czaja R,Struhalla M,Höschler K,Saenger W,Sträter N,Hahn U

更新日期：2004-03-16 00:00:00

abstract：:In a previous report, the cDNA for human proteinase inhibitor 8 (PI8) was first identified, isolated, and subcloned into a mammalian expression vector and expressed in baby hamster kidney cells. Initial studies indicated that PI8 was able to inhibit the amidolytic activity of trypsin and form an SDS-stable approximate...

journal_title：Biochemistry

authors： Dahlen JR,Foster DC,Kisiel W

更新日期：1997-12-02 00:00:00

abstract：:The S100 proteins comprise a group of EF-hand proteins that undergo a calcium-induced conformational change allowing them to interact with other proteins and produce a biological response. A unique feature of these proteins is the fact that they can form both homo- and heterodimers independent of calcium binding. The ...

journal_title：Biochemistry

authors： Marlatt NM,Boys BL,Konermann L,Shaw GS

更新日期：2009-03-10 00:00:00

abstract：:We propose a simple method to find an amino acid sequence that is foldable into a globular protein with a desired structure based on a knowledge-based 3D-1D compatibility function. An asymmetric alpha-helical single-domain structure of sperm whale myoglobin consisting of 153 amino acid residues was chosen for the desi...

journal_title：Biochemistry

authors： Isogai Y,Ota M,Fujisawa T,Izuno H,Mukai M,Nakamura H,Iizuka T,Nishikawa K

更新日期：1999-06-08 00:00:00

abstract：:RNA:pseudouridine synthetase (Pus1) from Saccharomyces cerevisiae is a multisite specific enzyme that catalyzes the formation of pseudouridine at positions 34 and 36 of intron-containing precursor tRNAIle and at positions 27 and/or 28 of several yeast tRNAs. In this paper we demonstrate that the purified recombinant P...

journal_title：Biochemistry

authors： Arluison V,Hountondji C,Robert B,Grosjean H

更新日期：1998-05-19 00:00:00

abstract：:The intermediates in the oxidation of deoxyhemoglobin by ferricyanide in 0.1 M KCl, at 20 degrees C and three pH values, were studied by cryogenic techniques. Data analysis was carried out according to a simple four rate constant model, ignoring the functional heterogeneity of the subunits, to simulate the time course...

journal_title：Biochemistry

authors： Perrella M,Shrager RI,Ripamonti M,Manfredi G,Berger RL,Rossi-Bernardi L

更新日期：1993-05-18 00:00:00

abstract：:Effects of different end sequences on melting, circular dichroism spectra (CD), and enzyme binding properties were investigated for four 40 base pair, non-self-complementary duplex DNA oligomers. The center sequences of these oligoduplexes have either of two 22 base pair modules flanked on both sides by sequences diff...

journal_title：Biochemistry

authors： Vallone PM,Benight AS

更新日期：2000-07-04 00:00:00

abstract：:Mammalian ribonucleotide reductase consists of two nonidentical subunits, proteins R1 and R2, each inactive alone. The R1 protein binds the ribonucleotide substrates while the R2 protein contains a binuclear iron center and a tyrosyl free radical, essential for activity. The crystal structures of the corresponding Esc...

journal_title：Biochemistry

authors： Rova U,Goodtzova K,Ingemarson R,Behravan G,Gräslund A,Thelander L

更新日期：1995-04-04 00:00:00

abstract：:Studies utilizing the overexpression of individual isoforms indicated that both PKC-alpha and -delta promote a number of biological effects, including inhibition of DNA synthesis associated with rearrangements of the actin cytoskeleton in the murine B-cell lymphoma (Baf3), differentiation of the murine promyelocyte li...

journal_title：Biochemistry

authors： Romanova LY,Alexandrov IA,Nordan RP,Blagosklonny MV,Mushinski JF

更新日期：1998-04-21 00:00:00

abstract：:Classical molecular dynamics simulations were utilized to investigate the structural and dynamical properties of water in the active site of ketosteroid isomerase (KSI) to provide insight into the role of these water molecules in the enzyme-catalyzed reaction. This reaction is thought to proceed via a dienolate interm...

journal_title：Biochemistry

authors： Hanoian P,Hammes-Schiffer S

更新日期：2011-08-09 00:00:00

abstract：:Synthetic ligands containing up to six residues of nonreducing terminal galactose were prepared. The synthesis involved coupling of carboxyl groups of N-benzyloxy-carbonylaspartic acid or of N-benzyloxycarbonyltyrosyl-gamma-glutamylglutamic acid to the omega-amino group of the aglycon of a glycoside that contained up ...

journal_title：Biochemistry

authors： Lee RT,Lin P,Lee YC

更新日期：1984-08-28 00:00:00

abstract：:N-Acyl ethanolamines (NAEs) constitute a large and diverse class of signaling lipids that includes the endogenous cannabinoid anandamide. Like other lipid transmitters, NAEs are thought to be biosynthesized and degraded on-demand rather than being stored in vesicles prior to signaling. The identification of enzymes in...

journal_title：Biochemistry

authors： Leung D,Saghatelian A,Simon GM,Cravatt BF

更新日期：2006-04-18 00:00:00

abstract：:Endonexin (annexin IV) is a member of the annexin family of homologous proteins that bind membranes and aggregate vesicles in a calcium-dependent fashion. This study examines the lipid modulation and mechanism of the binding of endonexin to membranes using a fluorescence energy transfer assay to measure bovine endonex...

journal_title：Biochemistry

authors： Junker M,Creutz CE

更新日期：1994-08-02 00:00:00

abstract：:Metabolomics is a rapidly growing field of research used in the identification and quantification of the small molecule metabolites within an organism, thereby providing insights into cell metabolism and bioenergetics as well as processes important in clinical medicine, such as disposition of pharmaceutical compounds....

journal_title：Biochemistry

authors： Milne SB,Mathews TP,Myers DS,Ivanova PT,Brown HA

更新日期：2013-06-04 00:00:00

abstract：:GTPases are key players during cellular signaling. Phosphorylation of Rab proteins, which belong to the Ras superfamily of small GTPases regulating intracellular transport, has been implicated in the pathogenesis of Parkinson's disease. For Rab8a, it was shown that serine 111 phosphorylation (pS111) is dependent on th...

journal_title：Biochemistry

authors： Pourjafar-Dehkordi D,Vieweg S,Itzen A,Zacharias M

更新日期：2019-08-20 00:00:00

abstract：:The transfer of ganglioside GM1 from micelles to membranes and between different membrane populations has been examined by using a pyrene fatty acid derivative of the ganglioside. The transfer of gangliosides from micelles to membranes depends on the physical state as well as the molecular composition of the acceptor ...

journal_title：Biochemistry

authors： Masserini M,Freire E

更新日期：1987-01-13 00:00:00

abstract：:A likely entry/exit path for nitrogenase substrates, products, and/or protons involves residues α277(Arg), α192(Ser), and α356(Gly), all of which are highly conserved among MoFe proteins from different organisms. The α192(Ser) and α277(Arg) residues form part of a hydrogen-bonded network that also involves α195(His), ...

journal_title：Biochemistry

authors： Fisher K,Hare ND,Newton WE

更新日期：2014-10-07 00:00:00

abstract：:Wheat germ ribosomes combine with the AUG codon at positions 30-32 from the 5' terminus of in vitro radioiodinated satellite tobacco necrosis virus (STNV) ribonucleic acid (RNA) to form initiation complexes that protect specific regions of the RNA from attack by ribonucleases. Wheat gern 80S ribosomes convey partial p...

journal_title：Biochemistry

authors： Browning KS,Leung DW,Clark JM Jr

更新日期：1980-05-13 00:00:00

abstract：:According to previous X-ray diffraction studies, the D85N mutant of bacteriorhodopsin (bR) with unprotonated Schiff base assumes a protein conformation similar to that in the M photointermediate. We recorded (13)C NMR spectra of [3-(13)C]Ala- and [1-(13)C]Val-labeled D85N and D85N/D96N mutants at ambient temperature t...

journal_title：Biochemistry

authors： Kawase Y,Tanio M,Kira A,Yamaguchi S,Tuzi S,Naito A,Kataoka M,Lanyi JK,Needleman R,Saitô H

更新日期：2000-11-28 00:00:00

abstract：:Phosphorus NMR spectroscopy is an important technique for the investigation of metabolism in tissues and intact organisms (including man). However, quantitation of the signals from an NMR experiment is difficult because it is not known from which regions of a cell metabolites are detected. It is generally believed tha...

journal_title：Biochemistry

authors： Jeffrey FM,Storey CJ,Nunnally RL,Malloy CR

更新日期：1989-06-27 00:00:00

abstract：:Recent studies of DNA hairpin loops have shown considerable dependence of the stability on the sequence of the loop [Senior, M., Jones, R. A., & Breslauer, K. J. (1988a) Proc. Natl. Acad. Sci. U.S.A. 85, 6242-6246; Xodo, L. E., Manzini, G., Quadrifoglio, F., van der Marel, G., & van Boom, J. H. (1989) Biochimie 71, 79...

journal_title：Biochemistry

authors： Wang S,Booher MA,Kool ET

更新日期：1994-04-19 00:00:00

abstract：:Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (EC 5.3.3.2) catalyzes the antarafacial [1.3] allylic rearrangement of isopentenyl diphosphate (IPP) to its electrophilic allylic isomer dimethylallyl diphosphate (DMAPP). Active-site thiols at C138 and C139 were recently identified by covalent modification u...

journal_title：Biochemistry

authors： Street IP,Coffman HR,Baker JA,Poulter CD

更新日期：1994-04-12 00:00:00

abstract：:Familial British dementia (FBD) is a rare neurodegenerative disorder and shares features with Alzheimer's disease, including amyloid plaque deposits, neurofibrillary tangles, neuronal loss, and progressive dementia. Immunohistochemical and biochemical analysis of plaques and vascular amyloid of FBD brains revealed tha...

journal_title：Biochemistry

authors： El-Agnaf OM,Sheridan JM,Sidera C,Siligardi G,Hussain R,Haris PI,Austen BM

更新日期：2001-03-27 00:00:00

abstract：:The importance of the evolutionarily conserved Gly-4 residue for the affinity and kinetics of interaction of cystatin A with several cysteine proteinases was assessed by site-directed mutagenesis. Even the smallest replacement, by Ala, resulted in approximately 1000-, approximately 10- and approximately 6000-fold decr...

journal_title：Biochemistry

authors： Estrada S,Nycander M,Hill NJ,Craven CJ,Waltho JP,Björk I

更新日期：1998-05-19 00:00:00

abstract：:We have developed a panel of antibodies specific for the alpha 1, alpha 2, alpha 3, and beta subunits of the rat Na,K-ATPase. TrpE-alpha subunit isoform fusion proteins were used to generate three antisera, each of which reacted specifically with a distinct alpha subunit isotype. Western blot analysis of rat tissue mi...

journal_title：Biochemistry

authors： Shyjan AW,Levenson R

更新日期：1989-05-30 00:00:00

abstract：:Retinal rod and cone cGMP phosphodiesterases (PDE6 family) function as the effector enzyme in the vertebrate visual transduction cascade. The activity of PDE6 catalytic subunits is controlled by the Pgamma-subunits. In addition to the inhibition of cGMP hydrolysis at the catalytic sites, Pgamma is known to stimulate a...

journal_title：Biochemistry

authors： Muradov KG,Granovsky AE,Schey KL,Artemyev NO

更新日期：2002-03-26 00:00:00

abstract：:Strong absorbance of water at 1645 cm-1 has previously prevented dependable observation of infrared spectra of H2O solutions in the carbonyl region. We have obtained reliable spectra in pure water and in alcohol-water mixtures in this region and used them to study A.U polynucleotide interaction. Spectra in pure water ...

journal_title：Biochemistry

authors： Miles HT,Frazier J

更新日期：1978-07-11 00:00:00

abstract：:The EcoRV restriction/modification system consists of two enzymes that recognize the DNA sequence GATATC. The EcoRV restriction endonuclease cleaves DNA at this site, but the DNA of Escherichia coli carrying the EcoRV system is protected from this reaction by the EcoRV methyltransferase. However, in vitro, the EcoRV n...

journal_title：Biochemistry

authors： Taylor JD,Goodall AJ,Vermote CL,Halford SE

更新日期：1990-12-04 00:00:00

abstract：:Kinetic models are presented that allow the Na(+),K(+)-ATPase steady-state turnover number to be estimated at given intra- and extracellular concentrations of Na(+), K(+), and ATP. Based on experimental transient kinetic data, the models utilize either three or four steps of the Albers-Post scheme, that is, E(2) --> E...

journal_title：Biochemistry

authors： Kong BY,Clarke RJ

更新日期：2004-03-02 00:00:00

abstract：:Trytophanase from Bacillus alvei was resolved from its coenzyme, pyridoxal phosphate, by treatment with cysteine followed by column chromatography. Spectrophotometric titration of apoenzyme with pyridoxal-P showed 1 mol of pyridoxal-P bound per 52,000 g of enzyme. Kinetic analysis of coenzyme binding showed hyperbolic...

journal_title：Biochemistry

authors： Isom HC,DeMoss RD

更新日期：1975-09-23 00:00:00