Abstract:
:We have developed a panel of antibodies specific for the alpha 1, alpha 2, alpha 3, and beta subunits of the rat Na,K-ATPase. TrpE-alpha subunit isoform fusion proteins were used to generate three antisera, each of which reacted specifically with a distinct alpha subunit isotype. Western blot analysis of rat tissue microsomes revealed that alpha 1 subunits were expressed in all tissues while alpha 2 subunits were expressed in brain, heart, and lung. The alpha 3 subunit, a protein whose existence had been inferred from cDNA cloning, was expressed primarily in brain and copurified with ouabain-inhibitable Na,K-ATPase activity. An antiserum specific for the rat Na,K-ATPase beta subunit was generated from a TrpE-beta subunit fusion protein. Western blot analysis showed that beta subunits were present in kidney, brain, and heart. However, no beta subunits were detected in liver, lung, spleen, thymus, or lactating mammary gland. The distinct tissue distributions of alpha and beta subunits suggest that different members of the Na,K-ATPase family may have specialized functions.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Shyjan AW,Levenson Rdoi
10.1021/bi00437a002subject
Has Abstractpub_date
1989-05-30 00:00:00pages
4531-5issue
11eissn
0006-2960issn
1520-4995journal_volume
28pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::In the globin family, similarities in the folding mechanism have been found among different mammalian apomyoglobins (apoMb). The best-characterized intermediate of sperm whale apoMb, called I(AGH), is mainly stabilized by nativelike contacts among the A, G, and H helices involving a cluster of hydrophobic residues tha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035319l
更新日期:2004-01-13 00:00:00
abstract::The reaction of ferric cytochrome c peroxidase (CcP) from Saccharomyces cerevisiae with peroxide produces compound I, characterized by both an oxyferryl iron center and a protein-based free radical. The electron paramagnetic resonance (EPR) signal of the CcP compound I radical can be resolved into a broad majority com...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00221a036
更新日期:1991-02-19 00:00:00
abstract::Photoactive yellow proteins (PYPs) make up a diverse class of blue-light-absorbing bacterial photoreceptors. Electronic excitation of the p-coumaric acid chromophore covalently bound within PYP results in triphasic quenching kinetics; however, the molecular basis of this behavior remains unresolved. Here we explore th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01114
更新日期:2018-03-20 00:00:00
abstract::The bacteriophage T7 DNA helicase/primase (gene 4 protein) is a ring-like hexamer that encircles ssDNA and requires forked DNA to catalyze DNA unwinding. We report that optimal rates of unwinding of forked DNA require ssDNA tails of 55 nucleotides on the 5'-to-3' strand and 15 nucleotides on the 3'-to-5' strand. Surpr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971644v
更新日期:1997-11-18 00:00:00
abstract::Diphtheria toxin (DT) has been studied as a model for understanding active-site structure and function in the ADP-ribosyltransferases. Earlier evidence suggested that histidine-21 of DT is important for the ADP-ribosylation of eukaryotic elongation factor 2 (EF-2). We have generated substitutions of this residue by ca...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00183a019
更新日期:1994-05-03 00:00:00
abstract::The catalysis of the chemical exchange between acetaldehyde and its hydrate by bovine carbonic anhydrase B was investigated by the NMR line broadening and saturation transfer techniques. While both chemical exchange and binding to the enzyme had an effect on the line broadening of acetaldehyde and its hydrate, the sat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00550a021
更新日期:1980-04-29 00:00:00
abstract::In bacteria, regulatory phosphorylation of proteins at serine and/or threonine residues by Ser/Thr protein kinase (STPK) is an emerging theme in prokaryotic signaling, particularly since the prediction of the occurrence of several STPKs from genome sequencing and sequence surveys. Here we show that protein PknH posses...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035150b
更新日期:2003-12-30 00:00:00
abstract::Methods were developed for isolation of bovine cardiac titin in the apparently full-sized T1 form. The pure protein could be obtained either in the denatured state after chromatography of myofibril extracts on Sephacryl S-1000 or in the native state using hydroxylapatite chromatography. Titin-specific chicken polyclon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00193a012
更新日期:1994-07-12 00:00:00
abstract::Actinaria cytolysins are very potent basic toxins isolated from the venom of sea anemones, which are supposed to exert their toxic activity through formation of oligomeric pores in the host plasma membrane. To gain insight into their mechanism of action, the interaction of Stichodactyla helianthus sticholysin I (St-I)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960787z
更新日期:1996-11-26 00:00:00
abstract::A mass transport theory of the gel chromatographic procedure of Hummel and Dreyer for measuring the binding of small ligand molecules to macromolecules has been formulated for ligand-mediated macromolecular association. It is concluded that the Hummel-Dreyer procedure is the method of choice for quantitating ligand bi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00666a011
更新日期:1976-10-19 00:00:00
abstract::The S2 subsite specificity of the plant protease papain has been altered to resemble that of mammalian cathepsin B by site-directed mutagenesis. On the basis of amino acid sequence alignments for papain and cathepsin B, a double mutant (Val133Ala/Ser205Glu) was produced where Val133 and Ser205 are replaced by Ala and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00101a003
更新日期:1991-09-17 00:00:00
abstract::X-ray reflectivity was used to study the interaction of the PX domain of p40(phox) protein (p40(phox)-PX) with a Langmuir monolayer of a mixture of SOPC (1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine), SOPS (1-stearoyl-2-oleoyl-sn-glycero-3-phosphoserine), and DPPtdIns(3)P (1,2-dipalmitoylphosphatidylinositol 3-phos...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061133l
更新日期:2006-11-14 00:00:00
abstract::Polylysine promoted extensive membrane mixing of liposomes only if the buffer pH was below the pKa of the lysyl residues. This observation suggested that fusion could be regulated in a physiological pH range if the homopolymer of L-histidine was substituted as fusogen. Microgram quantities of polyhistidine were added ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00322a001
更新日期:1985-01-01 00:00:00
abstract::Post-translational phosphorylation and the related conformational changes in signaling proteins are responsible for regulating a wide range of subcellular processes. Human Pin1 is central to many of these cell signaling pathways in normal and aberrant subcellular processes, catalyzing cis-trans isomerization of the pe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi2009954
更新日期:2011-11-08 00:00:00
abstract::To determine if mutations introduced into phospholemman (PLM) could increase the level of PLM-Na,K-ATPase (NKA) binding, we performed scanning mutagenesis of the transmembrane domain of PLM and measured Förster resonance energy transfer (FRET) between each mutant and NKA. We observed an increased level of binding to N...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00633
更新日期:2016-11-08 00:00:00
abstract::The removal of damaged bases by DNA glycosylases is thought to be effectively irreversible, because of an overall equilibrium that favors hydrolysis over synthesis of the N-glycosyl bond. Surprisingly, human alkyladenine DNA glycosylase (AAG) can make damaged DNA by catalyzing formation of an N-glycosyl bond between 1...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101380d
更新日期:2010-10-26 00:00:00
abstract::The value of reducing the activity of ornithine decarboxylase (ODC), a key enzyme in the biosynthesis of polyamines, is well-appreciated. Polyamines are necessary components for cell growth, and manipulation of polyamine homeostasis may be an effective strategy for the treatment of a number of disorders, including neo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047918d
更新日期:2005-02-15 00:00:00
abstract::Merozoite surface protein 2 (MSP2), an abundant glycosylphosphatidylinositol-anchored protein on the surface of Plasmodium falciparum merozoites, is a promising malaria vaccine candidate. MSP2 is intrinsically disordered and forms amyloid-like fibrils in solution under physiological conditions. The 25 N-terminal resid...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201880s
更新日期:2012-02-21 00:00:00
abstract::A newly recognized type of protein-ligand interaction phenomenon has resulted in the preparation of simple, nonionic, and highly specific gel derivatives for selective adsorption chromatography. The essential structure of the immobilized ligand can be represented as agarose-CH2CH2SO2CH2CH2SCH2CH2OH, which was prepared...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00396a049
更新日期:1987-11-03 00:00:00
abstract::The potassium form of d(CGCGAATTCGCG) solved by X-ray diffraction to 1.75 A resolution indicates that monovalent cations penetrate the primary and secondary layers of the "spine of hydration". Both the sodium [Shui, X., McFail-Isom, L., Hu, G. G., and Williams, L. D. (1998) Biochemistry 37, 8341-8355] and the potassiu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982063o
更新日期:1998-12-01 00:00:00
abstract::We synthesized the 26-residue deoxynucleotide sequence d(TTCCT5GGAATTCCT5GGAA) which folds intramolecularly to form a dumbbell-shaped, double-hairpin structure with a gap between the 3' and the 5' ends. We used T4 polynucleotide kinase to phosphorylate the 5' end followed by T4 DNA ligase to close the 3' and 5' ends. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00427a037
更新日期:1989-01-10 00:00:00
abstract::We have modified the cytochrome b subunit of the cytochrome bc1 complex from the purple bacterium Rhodobacter sphaeroides to introduce two distinctive features of cytochrome b6 f complexes. In the first one, we have split cyt b into two polypeptides thus mimicking the organization of cyt b6 and subunit IV in the b6 f ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9813476
更新日期:1998-11-17 00:00:00
abstract::Perioperative bleeding is a cause of major blood loss and is associated with increased rates of postoperative morbidity and mortality. To combat this, antifibrinolytic inhibitors of the serine protease plasmin are commonly used to reduce bleeding during surgery. The most effective and previously widely used of these i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201203y
更新日期:2011-10-04 00:00:00
abstract::ADP-glucose pyrophosphorylase (ADPGlc PPase) catalyzes the conversion of glucose 1-phosphate and ATP to ADP-glucose and pyrophosphate. As a key step in glucan synthesis, the ADPGlc PPases are highly regulated by allosteric activators and inhibitors in accord with the carbon metabolism pathways of the organism. Crystal...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701933q
更新日期:2008-04-15 00:00:00
abstract::TPPP/p25 is a brain-specific protein, which induces tubulin polymerization and microtubule (MT) bundling and is enriched in Lewy bodies characteristic of Parkinson's disease [Tirián et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 13976-13981]. We identified two human gene sequences, CG1-38 and p25beta, which encoded ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061305e
更新日期:2006-11-21 00:00:00
abstract::Factor V and factor Va binding to single bilayer phospholipid vesicles was investigated by light-scattering intensity measurements. This technique allows the measurement of free and phospholipid-bound protein concentrations from which equilibrium constants can be obtained. As controls, the Ca2+-dependent phospholipid ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00587a023
更新日期:1979-10-02 00:00:00
abstract::Human angiotensin-converting enzyme (ACE) has two homologous domains, the N and C domains, with differing substrate preferences. X-ray crystal structures of the C and N domains complexed with various inhibitors have allowed identification of active site residues that might be important for the molecular basis of this ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8002605
更新日期:2008-06-03 00:00:00
abstract::The aminoacylase-1/metallopeptidase 20 (Acy1/M20) family features several l-aminoacylases useful in biocatalysis. Mammalian Acy1, in particular, has been applied in racemic resolution and reverse hydrolysis. Despite recent advances in our understanding of the active site architecture and functioning, determinants of A...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi702156h
更新日期:2008-04-08 00:00:00
abstract::ZntA from Escherichia coli confers resistance to toxic concentrations of Pb2+, Zn2+, and Cd2+. It is a member of the P1B-ATPase transporter superfamily, which includes the human Cu+-transporting proteins ATP7A and ATP7B. P1B-type ATPases typically have a hydrophilic N-terminal metal-binding domain and eight transmembr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00770
更新日期:2020-12-01 00:00:00
abstract::Familial British dementia (FBD) is a rare neurodegenerative disorder and shares features with Alzheimer's disease, including amyloid plaque deposits, neurofibrillary tangles, neuronal loss, and progressive dementia. Immunohistochemical and biochemical analysis of plaques and vascular amyloid of FBD brains revealed tha...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002287i
更新日期:2001-03-27 00:00:00