Hummel-Dreyer gel chromatographic procedure as applied to ligand-mediated association.

abstract：:The dynamic cytoskeletal network of microtubules and actin filaments can be disassembled by drugs. Cytoskeletal drugs work by perturbing the monomer-polymer equilibrium, thus changing the size and number of macromolecular crowders inside cells. Changes in both crowding and nonspecific surface interactions ("sticking")...

journal_title：Biochemistry

authors： Davis CM,Gruebele M

更新日期：2020-07-21 00:00:00

abstract：:Escherichia coli PurK, a dimeric N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) synthetase, catalyzes the conversion of 5-aminoimidazole ribonucleotide (AIR), ATP, and bicarbonate to N5-CAIR, ADP, and Pi. Crystallization of both a sulfate-liganded and the MgADP-liganded E. coli PurK has resulted in structures at 2....

journal_title：Biochemistry

authors： Thoden JB,Kappock TJ,Stubbe J,Holden HM

更新日期：1999-11-23 00:00:00

abstract：:1H NMR spectral editing techniques can select the distinct signals of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate and provide a unique way to monitor the biochemical processes in vivo. These metabolite levels reflect the near-equilibrium dehydrogenase activity and therefore the cellular redox state. The ...

journal_title：Biochemistry

authors： Chung Y,Jue T

更新日期：1992-11-17 00:00:00

abstract：:Thioredoxin reductase (TrxR) catalyzes the reduction of thioredoxin (Trx) by NADPH. A unique gene organization of TrxR and Trx has been found in Mycobacterium leprae, where TrxR and Trx are encoded by a single gene and, therefore, are expressed as a fusion protein (MlTrxR-Trx). This fusion enzyme is able to catalyze t...

journal_title：Biochemistry

authors： Wang PF,Marcinkeviciene J,Williams CH Jr,Blanchard JS

更新日期：1998-11-17 00:00:00

abstract：:The site-directed mutagenesis of a number of proposed active site residues of 5-enolpyruvyl shikimate-3-phosphate (EPSP) synthase is reported. Several of these mutations resulted in complete loss of enzyme activity indicating that these residues are probably involved with catalysis, notably K22R, K411R, D384A, R27A, R...

journal_title：Biochemistry

authors： Shuttleworth WA,Pohl ME,Helms GL,Jakeman DL,Evans JN

更新日期：1999-01-05 00:00:00

abstract：:Incubation of 3-(2-phenylethyl)-4-methylsydnone (PMS) with liver microsomes from phenobarbital-pretreated rats or with reconstituted cytochrome P-450b results in loss of the enzyme chromophore. Chromophore loss is NADPH-dependent even though the sydnone decomposes by an oxygen- but not enzyme-dependent process to give...

journal_title：Biochemistry

authors： Grab LA,Swanson BA,Ortiz de Montellano PR

更新日期：1988-06-28 00:00:00

abstract：:We find that the isolated, extracellular domain of tissue factor (TF1-218; sTF) exhibits only 4% of the activity of wild-type transmembrane TF (TF1-263) in an assay that measures the conversion of factor X to Xa by the TF:VIIa complex. Further, the activity of sTF is manifest only when vesicles consisting of phosphati...

journal_title：Biochemistry

authors： Waxman E,Ross JB,Laue TM,Guha A,Thiruvikraman SV,Lin TC,Konigsberg WH,Nemerson Y

更新日期：1992-04-28 00:00:00

abstract：:Protein-lipid interactions regulate many membrane protein functions. Using a multiscale approach that combines coarse-grained and atomistic molecular dynamics simulations, we have predicted the binding site for the anionic phospholipid phosphatidylinositol 4,5-bisphosphate (PIP(2)) on the Kir2.2 inwardly rectifying (K...

journal_title：Biochemistry

authors： Schmidt MR,Stansfeld PJ,Tucker SJ,Sansom MS

更新日期：2013-01-15 00:00:00

abstract：:The interaction between cytochrome c oxidase complex and adenosine triphosphate synthase (F1F0) complex in the purified, dispersed state and embedded in phospholipid vesicles was studied by differential scanning calorimetry and by spin-label electron paramagnetic resonance. The detergent-dispersed cytochrome oxidase a...

journal_title：Biochemistry

authors： Qiu ZH,Yu L,Yu CA

更新日期：1992-03-31 00:00:00

abstract：:Depletion of Ca2+ and/or Cl- ions from PSII membranes blocks the electron-transfer reactions that precede O2 evolution on the oxidizing side of the enzyme. Illumination of these inhibited preparations at 273 K generates a paramagnetic species that is detectable by low-temperature (T < 20 K) EPR as a signal in the g = ...

journal_title：Biochemistry

authors： Lydakis-Simantiris N,Dorlet P,Ghanotakis DF,Babcock GT

更新日期：1998-05-05 00:00:00

abstract：:β-Barrel membrane proteins are found in the outer membranes of mitochondria, chloroplasts, and Gram-negative bacteria; however, exactly how they are folded and inserted remains unknown. Over the past decade, both functional and structural studies have greatly contributed to addressing this elusive mechanism. It is kno...

journal_title：Biochemistry

authors： O'Neil PK,Rollauer SE,Noinaj N,Buchanan SK

更新日期：2015-10-20 00:00:00

abstract：:Protein amyloid fibrils are originally identified as pathological entities in a variety of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Recent studies have revealed that amyloid fibrils also serve as functional protein assemblies to fulfill a wide range of biological functions. Decip...

journal_title：Biochemistry

authors： Li D,Liu C

更新日期：2020-02-11 00:00:00

abstract：:SufS is a type II cysteine desulfurase and acts as the initial step in the Suf Fe-S cluster assembly pathway. In Escherichia coli, this pathway is utilized under conditions of oxidative stress and is resistant to reactive oxygen species. Mechanistically, this means SufS must shift between protecting a covalent persulf...

journal_title：Biochemistry

authors： Dunkle JA,Bruno MR,Outten FW,Frantom PA

更新日期：2019-02-12 00:00:00

abstract：:Subunits located near the cardiolipin binding sites of bovine heart cytochrome c oxidase (CcO) were identified by photolabeling with arylazido-cardiolipin analogues and detecting labeled subunits by reversed-phase HPLC and HPLC-electrospray ionization mass spectrometry. Two arylazido-containing cardiolipin analogues w...

journal_title：Biochemistry

authors： Sedlák E,Panda M,Dale MP,Weintraub ST,Robinson NC

更新日期：2006-01-24 00:00:00

abstract：:We investigated the dependence of certain properties of bile salt/lecithin mixed micelles on the molar ratio of the two components. UV absorption suggested that the micellar system is fundamentally altered on passing through a bile salt:lecithin molar ratio of 1.8:1. Differential scanning calorimetry confirmed this tr...

journal_title：Biochemistry

authors： Claffey WJ,Holzbach RT

更新日期：1981-01-20 00:00:00

abstract：:Yeast (Saccharomyces cerevisiae) alcohol dehydrogenase I (ADH1) is the constitutive enzyme that reduces acetaldehyde to ethanol during the fermentation of glucose. ADH1 is a homotetramer of subunits with 347 amino acid residues. A structure for ADH1 was determined by X-ray crystallography at 2.4 Å resolution. The asym...

journal_title：Biochemistry

authors： Raj SB,Ramaswamy S,Plapp BV

更新日期：2014-09-16 00:00:00

abstract：:Human cystathionine beta-synthase (CBS) is an essential enzyme for the removal of the toxic metabolite homocysteine. Heme and pyridoxal phosphate (PLP) cofactors are necessary to catalyze the condensation of homocysteine and serine to generate cystathionine. While the role for the PLP cofactor is thought to be similar...

journal_title：Biochemistry

authors： Green EL,Taoka S,Banerjee R,Loehr TM

更新日期：2001-01-16 00:00:00

abstract：:Here we demonstrate that monovalent cations can localize around B-DNA in geometrically regular, sequence-specific sites in oligonucleotide crystals. Positions of monovalent ions were determined from high-resolution X-ray diffraction of DNA crystals grown in the presence of thallium(I) cations (Tl(+)). Tl(+) has previo...

journal_title：Biochemistry

authors： Howerton SB,Sines CC,VanDerveer D,Williams LD

更新日期：2001-08-28 00:00:00

abstract：:Tropomyosins from bovine aorta and pulmonary artery exhibit identical electrophoretic patterns in sodium dodecyl sulfate but differ from tropomyosins of either chicken gizzard or rabbit skeletal muscle. Each of the four tropomyosins binds readily to skeletal muscle F-actin as indicated by their sedimentation with acti...

journal_title：Biochemistry

authors： Yamaguchi M,Ver A,Carlos A,Seidel JC

更新日期：1984-02-14 00:00:00

abstract：:Cadherins are single pass transmembrane glycoproteins which mediate calcium dependent cell-cell adhesion by homophilic interactions. To reveal the molecular details of calcium binding and homoassociation, we recombinantly expressed in Escherichia coli a domain pair consisting of the first two domains of E-cadherin (EC...

journal_title：Biochemistry

authors： Koch AW,Pokutta S,Lustig A,Engel J

更新日期：1997-06-24 00:00:00

abstract：:The DNA binding domain (DBD) of p53 folds by a complex mechanism that involves parallel pathways and multiple intermediates, both on- and off-pathway. This heterogeneity renders DBD particularly susceptible to misfolding and aggregation. The origins of parallel folding mechanisms are not well understood. DBD folding h...

journal_title：Biochemistry

authors： Butler JS,Loh SN

更新日期：2007-03-13 00:00:00

abstract：:The conformation of two fragments derived from anti-poly(D-alanyl) antibodies, the divalent fragment (Fab')2 and the monovalent fragment Fab', was studied by small-angle X-ray scattering before and after interaction with the tetra-D-alanine amide hapten. More than 90% of the combining sites were occupied by the hapten...

journal_title：Biochemistry

authors： Pilz I,Kratky O,Licht A,Sela M

更新日期：1975-03-25 00:00:00

abstract：:Mutations in human copper-zinc superoxide dismutase (SOD1) cause an inherited form of the fatal neurodegenerative disease amyotrophic lateral sclerosis (ALS). Here, we present structures of the pathogenic SOD1 variants D124V and H80R, both of which demonstrate compromised zinc-binding sites. The disruption of the zinc...

journal_title：Biochemistry

authors： Seetharaman SV,Winkler DD,Taylor AB,Cao X,Whitson LJ,Doucette PA,Valentine JS,Schirf V,Demeler B,Carroll MC,Culotta VC,Hart PJ

更新日期：2010-07-13 00:00:00

abstract：:Chemical modification, mutagenesis, chemical rescue, and isotope effect studies are used to identify and probe the roles of several conserved amino acid groups in catalysis by human dihydroorotate dehydrogenase. Time- and pH-dependent inactivation of human dihydroorotate dehydrogenase by trinitrobenzenesulfonate impli...

journal_title：Biochemistry

authors： Jiang W,Locke G,Harpel MR,Copeland RA,Marcinkeviciene J

更新日期：2000-07-11 00:00:00

abstract：:Alpha-synuclein (alpha-syn) is the major component of intracellular inclusions in several neurodegenerative diseases, and the conversion of soluble alpha-syn into filamentous aggregates may contribute to disease pathogenesis. Since mechanisms leading to the formation of alpha-syn inclusions are unclear, in vitro model...

journal_title：Biochemistry

authors： Murray IV,Giasson BI,Quinn SM,Koppaka V,Axelsen PH,Ischiropoulos H,Trojanowski JQ,Lee VM

更新日期：2003-07-22 00:00:00

abstract：:The membrane interaction properties of two single-residue variants, R6W and L5S, of the 17-amino acid neuropeptide dynorphin A (DynA) were studied by circular dichroism (CD) and nuclear magnetic resonance (NMR) spectroscopy. Corresponding gene mutations have recently been discovered in humans and causatively linked to...

journal_title：Biochemistry

authors： Björnerås J,Gräslund A,Mäler L

更新日期：2013-06-18 00:00:00

abstract：:Triglyceride analogues were synthesized in which one of the primary acyl ester functions has been replaced by an alkyl group and the secondary acyl ester bond has been replaced by an acyl amino bond. The chain length at either position was varied, and both (R)- and (S)-enantiomers of each compound were synthesized. Th...

journal_title：Biochemistry

authors： Mannesse ML,Cox RC,Koops BC,Verheij HM,de Haas GH,Egmond MR,van der Hijden HT,de Vlieg J

更新日期：1995-05-16 00:00:00

abstract：:The structure of PsbQ, one of the three main extrinsic proteins associated with the oxygen-evolving complex (OEC) of higher plants and green algae, is examined by Fourier transform infrared (FTIR) and circular dichroic (CD) spectroscopy and by computational structural prediction methods. This protein, together with tw...

journal_title：Biochemistry

authors： Balsera M,Arellano JB,Gutiérrez JR,Heredia P,Revuelta JL,De Las Rivas J

更新日期：2003-02-04 00:00:00

abstract：:The clathrin assembly protein complex AP-2 is a multimeric subunit complex consisting of two 100-115-kDa subunits known as alpha and beta and 50- and 16-kDa subunits. The subunits have been dissociated and separated by ion-exchange chromatography in 7.5 M urea. Fractions highly enriched in either the alpha or beta sub...

journal_title：Biochemistry

authors： Prasad K,Keen JH

更新日期：1991-06-04 00:00:00

abstract：:In prokaryotes, a large share of the proteins are secreted from the cell through a process that requires their translocation across the cytoplasmic membrane. This process is mediated by the universally conserved Sec system with homologues in the endoplasmic reticulum and thylakoid membranes of eukaryotes. The Sec syst...

journal_title：Biochemistry

authors： Kedrov A,Kusters I,Driessen AJ

更新日期：2013-10-01 00:00:00