Abstract:
:In prokaryotes, a large share of the proteins are secreted from the cell through a process that requires their translocation across the cytoplasmic membrane. This process is mediated by the universally conserved Sec system with homologues in the endoplasmic reticulum and thylakoid membranes of eukaryotes. The Sec system also facilitates the membrane insertion of integral membrane proteins, an essential step along their folding pathway. In bacteria, the Sec system consists of the protein-conducting channel (SecYEG) that associates with soluble components, such as the motor protein SecA or translating ribosomes, and with integral membrane proteins, such as the heterotrimeric complex termed SecDFyajC and the YidC insertase. Over the past three decades, biochemical and structural studies have provided a comprehensive view of protein translocation, but the exact mechanistic details of this process remain to be resolved. For a number of other biomolecular systems, single-molecule biophysical analysis has efficiently complemented the conventional biochemical studies conducted in bulk, with high-sensitivity measurements probing the structure and dynamics of individual molecules in vitro and in vivo. Here, we review recent advances in studies of protein translocation employing single-molecule techniques with the aim of resolving molecular mechanisms, thereby providing a new and detailed view of the process.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Kedrov A,Kusters I,Driessen AJdoi
10.1021/bi400913xsubject
Has Abstractpub_date
2013-10-01 00:00:00pages
6740-54issue
39eissn
0006-2960issn
1520-4995journal_volume
52pub_type
杂志文章,评审相关文献
BIOCHEMISTRY文献大全abstract::The reactivity of the mitochondrial (pI = 5) isoenzyme of horse liver aldehyde dehydrogenase was determined by studying the effects of pH on steady-state velocity, burst magnitude, and molecular weight of the enzyme in the absence and presence of Mg2+ ions. The Mg2+ ion activation of the steady-state velocity at pH 7....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00524a049
更新日期:1981-10-13 00:00:00
abstract::X-ray absorption spectra are reported for the multi-Cu oxidase Rhus vernicifera laccase in oxidized and fully reduced forms and for laccase from which the type 2 Cu has been depleted (T2D). The structure of the Cu K edge for both preparations shows the presence of CuII and CuI in the oxidized and reduced states, respe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00310a008
更新日期:1984-07-17 00:00:00
abstract::Curcumin potentiates the phosphorylation-dependent activity of human cystic fibrosis transmembrane conductance regulator (CFTR) in Fe(3+)-dependent and Fe(3+)-independent manners. Although the Fe(3+)-dependent curcumin potentiation results from removal of endogenous inhibitory Fe(3+) at the interface of the regulatory...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00219
更新日期:2015-05-12 00:00:00
abstract::We have synthesized 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho(N-oleoyl)serine (N-acyl-PS) and 1,2-dioleoyl-sn-glycero-3-phospho-Tris (phosphatidyl-Tris) and have characterized both phospholipids by their chemical and chromatographic properties, as well as by their IR, 13C NMR, and 1H NMR spectra. Comparison of these d...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00221a003
更新日期:1991-02-19 00:00:00
abstract::Several snake venoms contain a phospholipase A2 in which position 49 in the active site is occupied by a lysine or a serine instead of the aspartate residue normally found. Although these proteins do not bind Ca2+ and are devoid of catalytic activity, they are still highly specific myotoxins and have recently been sho...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00014a021
更新日期:1995-04-11 00:00:00
abstract::A crude in vitro system which initiates chloroplast DNA synthesis near the D-loop site mapped by electron microscopy [Wu, M., Lou, J. K., Chang, D. Y., Chang, C. H., & Nie, Z. Q. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 6761-6765] consists of soluble proteins and proteins extracted from purified thylakoid membrane. In...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00218a034
更新日期:1991-01-29 00:00:00
abstract::The synthesis and secretion of several acute-phase proteins increases markedly following physiological stress. alpha 1-Acid glycoprotein (AGP), a major acute-phase reactant made by the liver, is strongly induced by inflammatory agents such as lipopolysaccharide (LPS). Nuclear run-on assay showed a 17-fold increase in ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00122a005
更新日期:1992-02-25 00:00:00
abstract::The virally encoded integrase protein carries out retroviral integration, which requires specific interactions with the two ends of the viral DNA, and also with host DNA that is the target of integration. We attached a photo-cross-linking agent to specific viral and target DNA sites to identify regions of the integras...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972949c
更新日期:1998-05-12 00:00:00
abstract::The infectious isoform of the prion protein (PrPSc) is derived from cellular PrP (PrPC) in a conversion reaction involving a dramatic reorganization of secondary and tertiary structure. While our understanding of the pathogenic role of PrPSc has grown, the normal physiologic function of PrPC still remains unclear. Usi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972827k
更新日期:1998-05-19 00:00:00
abstract::Bovine liver rhodanese, which catalyzes the transfer of sulfur atoms between a variety of sulfur donor and sulfur acceptor substrates, is inhibited by metal cyanide complexes [Volini, M., Van Sweringen, B., & Chen, F.-Sh. (1978) Arch. Biochem. Biophys. 191, 205-215]. Crystallographic studies are described which reveal...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00281a026
更新日期:1983-06-07 00:00:00
abstract::The amidotransferase family of enzymes utilizes the ammonia derived from the hydrolysis of glutamine for a subsequent chemical reaction catalyzed by the same enzyme. The ammonia intermediate does not dissociate into solution during the chemical transformations. A well-characterized example of the structure and mechani...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi990871p
更新日期:1999-06-22 00:00:00
abstract::Most A/T specific heterocyclic diamidine derivatives need at least four A/T base pairs for tight binding to the DNA minor groove. Addition of a GC base pair to A/T sequences typically causes a large decrease in binding constant. The ability to target biologically important sequences of DNA could be significantly incre...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801944g
更新日期:2009-02-24 00:00:00
abstract::The primary electron acceptor pheophytin (Pheo(D1)) plays a crucial role in regulation of forward and backward electron transfer in photosystem II (PSII). It is known that some cyanobacteria control the Pheo(D1) potential in high-light acclimation by exchanging the D1 proteins from different copies of the psbA genes. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9018829
更新日期:2010-01-26 00:00:00
abstract::Creatininase catalyzes the conversion of creatinine (a biosensor for kidney function) to creatine via a two-step mechanism: water addition followed by ring opening. Water addition is common to other known cyclic amidohydrolases, but the precise mechanism for ring opening is still under debate. The proton donor in this...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01032
更新日期:2017-12-05 00:00:00
abstract::Our recent demonstration that DNA polymerase X (Pol X), the DNA repair polymerase encoded by the African swine fever virus (ASFV), is extremely error prone during single-nucleotide gap filling led us to hypothesize that it might contribute to genetic variability in ASFV. For the infidelity of Pol X to be relevant, how...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047706g
更新日期:2005-06-14 00:00:00
abstract::GGAs are a family of vesicle-coating regulatory proteins that function in intracellular protein transport. A GGA molecule contains four domains, each mediating interaction with other proteins in carrying out intracellular transport. The GAT domain of GGAs has been identified as the structural entity that binds membran...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034334n
更新日期:2003-06-03 00:00:00
abstract::Biochemical experiments, using the well-defined human liver CYP2C9 expressed in yeast, and molecular modeling techniques were used to derive a predictive model for substrates of CYP2C9. The ability of 10 2-aroylthiophenes related to tienilic acid to act as substrates for CYP2C9 was studied. Four of them were original ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00033a007
更新日期:1995-08-22 00:00:00
abstract::An oligodeoxynucleotide containing four thymines and covalently attached to an acridine derivative through its 3'-phosphate [(Tp)4(CH2)5Acr] was synthesized. Its conformation in solution was investigated by proton magnetic resonance. Both intramolecular interactions between the acridine dye and thymines and intermolec...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00331a019
更新日期:1985-05-07 00:00:00
abstract::MRP1 (or ABCC1) is an ABC membrane protein that transports a wide range of natural products as well as glutathione (GSH)-, glucuronate-, and sulfate-conjugated metabolites. In addition, free GSH is required for MRP1 to transport several chemotherapeutic drugs. However, the mechanisms regulating the influence of GSH on...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0268807
更新日期:2003-03-25 00:00:00
abstract::Volumetric characteristics of protein recognition events determine the direction of pressure-induced shifts in the recognition reaction, while also providing insights into the structural, dynamic, and hydration changes. We report changes in volume, ΔV, and adiabatic compressibility, ΔK(S), accompanying the binding of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3006994
更新日期:2012-07-24 00:00:00
abstract::A combined N- and C-terminal truncation variant of human apolipoprotein A-I (apoA-I) was designed, expressed in Escherichia coli, isolated, and characterized. Hydrodynamic experiments yielded a weight average molecular weight of 34000, indicating apoA-I-(44-186) exists in solution predominantly as a dimer. An axial ra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0477135
更新日期:2005-03-22 00:00:00
abstract::The reduction of substrates catalyzed by nitrogenase utilizes an electron transfer (ET) chain comprised of three metalloclusters distributed between the two component proteins, designated as the Fe protein and the MoFe protein. The flow of electrons through these three metalloclusters involves ET from the [4Fe-4S] clu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201003a
更新日期:2011-11-01 00:00:00
abstract::The protein phosphatase 2C (PP2C) family represents one of the four major protein Ser/Thr phosphatase activities in mammalian cells and contains at least 13 distinct gene products. Although PP2C family members regulate a variety of cellular functions, mechanisms of regulation of their activities are largely unknown. H...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800067p
更新日期:2008-07-08 00:00:00
abstract::MutY protein, a DNA glycosylase found in Escherichia coli, recognizes dA:dG, dA:8-oxodG, and dA:dC mismatches in duplex DNA, excising the adenine moiety. We have investigated the mechanism of action of MutY, addressing several points of disagreement raised by previous studies of this enzyme. MutY forms a covalent inte...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981066y
更新日期:1998-09-08 00:00:00
abstract::Moderate pressure accelerates hydride transfer catalyzed by yeast alcohol dehydrogenase, indicative of a large negative volume of activation [Cho and Northrop (1999) Biochemistry 38, 7470-7475]. A comparison of the effects of pressure on the oxidation of normal versus dideuteriobenzyl alcohol generates a monophasic de...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992537z
更新日期:2000-03-07 00:00:00
abstract::A simple model system is described that allows measurement of equilibrium Ca2+ binding to multilamellar vesicle mixtures of palmitoyloleoylphosphatidylserine (P,O-PS) and dimyristoleoylphosphatidylcholine (MO,MO-PC). The constraint of the chemical equilibrium among aqueous Ca2+, hydrated P,O-PS/MO,MO-PC, and Ca(PS)2, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00488a013
更新日期:1990-09-11 00:00:00
abstract::A 35-amino acid peptide corresponding to the putative "zinc finger" sequence of primase was prepared to study its zinc binding properties. When zinc was added to the peptide, it was found that the fluorescence quantum yield of the single tyrosine increased by 46% and the average lifetime by 34%. The binding stoichiome...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9612778
更新日期:1997-01-21 00:00:00
abstract::Proton nuclear magnetic resonance and ultraviolet circular dichroism spectroscopy have been used to probe the conformational ensemble of the tandemly repeating tetrapeptide unit of the circumsporozoite coat protein of the malaria parasite Plasmodium falciparum. Peptides based on the Asn-Ala-Asn-Pro and Asn-Pro-Asn-Ala...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00486a008
更新日期:1990-08-28 00:00:00
abstract::The dynamics of the single-stranded, helix--coil transition of poly(adenylic acid) have been investigated by using the Raman laser temperature jump technique. The driving forces of this conformational transition have been probed by varying the cosolvent in mixed aqueous solutions. The rate of helix formation correlate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00549a025
更新日期:1980-04-15 00:00:00
abstract::The proton transport membrane protein bacteriorhodopsin has been biosynthetically labeled with [methyl-13C]methionine and studied by high-resolution 13C NMR after solubilization in the detergent Triton X-100. The nine methionine residues of bacteriorhodopsin give rise to four well-resolved 13C resonances, two of which...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00230a012
更新日期:1991-04-23 00:00:00