L30A Mutation of Phospholemman Mimics Effects of Cardiac Glycosides in Isolated Cardiomyocytes.

abstract：:The photocycle of 124 kDa phytochrome A from Avena sativa was studied by Fourier-transform infrared spectroscopy at low temperatures. Difference spectra between the parent state Pr and the intermediates of the Pr-->Pfr pathway, i.e. lumi-R, meta-Ra, and meta-Rc, and between Pfr and the intermediates of the Pfr-->Pr pa...

journal_title：Biochemistry

authors： Foerstendorf H,Mummert E,Schäfer E,Scheer H,Siebert F

更新日期：1996-08-20 00:00:00

abstract：:The interaction between skeletal myosin subfragment 1 (S1) and filamentous actin was examined at various intermediate states of the actomyosin ATPase cycle by chemical cross-linking experiments. Reaction of the actin-S1 complex with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide and N-hydroxysuccinimide generated pro...

journal_title：Biochemistry

authors： Van Dijk J,Fernandez C,Chaussepied P

更新日期：1998-06-09 00:00:00

abstract：:Aldehyde dehydrogenase 7A1 (ALDH7A1) is part of lysine catabolism and catalyzes the NAD(+)-dependent oxidation of α-aminoadipate semialdehyde to α-aminoadipate. Herein, we describe a structural study of human ALDH7A1 focused on substrate recognition. Five crystal structures and small-angle X-ray scattering data are re...

journal_title：Biochemistry

authors： Luo M,Tanner JJ

更新日期：2015-09-08 00:00:00

abstract：:We present spectroscopic evidence for the presence of low-barrier hydrogen bonds (LBHBs) in molecular complexes composed of carboxylic acids and 1-methylimidazole (1-MeIm) dissolved in aprotic organic solvents. A plot of the values of the low-field proton NMR chemical shifts versus the aqueous pKa of the carboxylic ac...

journal_title：Biochemistry

authors： Tobin JB,Whitt SA,Cassidy CS,Frey PA

更新日期：1995-05-30 00:00:00

abstract：:Two photoreactive apamin derivatives were prepared with an aryl azide [[(azidonitrophenyl)amino]acetate (ANPAA)] group coupled at different positions on the neurotoxin molecule. These ligands were used to identify membrane components in the environment of the neuronal binding site that is associated with a Ca2+-activa...

journal_title：Biochemistry

authors： Seagar MJ,Labbé-Jullié C,Granier C,Goll A,Glossmann H,Van Rietschoten J,Couraud F

更新日期：1986-07-15 00:00:00

abstract：:The SCF ubiquitin ligase associates with substrates through its F-box protein adaptor. Substrates are typically recognized through a defined phosphodegron. Here, we characterize the interaction of the F-box protein Saf1 with Prb1, one of its vacuolar protease substrates. We show that Saf1 binds the mature protein but ...

journal_title：Biochemistry

authors： Mark KG,Meza-Gutierrez F,Johnson JR,Newton BW,Krogan NJ,Toczyski DP

更新日期：2015-07-28 00:00:00

abstract：:An endonuclease II preparation from Escherichia coli makes single strand breaks in DNA which has been treated with the carcinogen 7-bromomethyl-12-methylbenz[alpha]anthracene. In addition, the enzyme preparation excises N6-(12-methylbenz[alpha]anthracenyl-7-methyl)adenine and N2-(12-methylbenz[alpha]anthracenyl-7-met...

journal_title：Biochemistry

authors： Kirtikar DM,Dipple A,Goldthwait DA

更新日期：1975-12-30 00:00:00

abstract：:Calcium protects a limited number of embryonic chick neural retina cell surface proteins from tryptic cleavage. One glycoprotein of Mr approximately 1.3 X 10(5) and pI approximately 4.8 (gp130) is present in intact retinas and, in the presence of Ca2+, is both resistant to tryptic cleavage and poorly iodinated. When c...

journal_title：Biochemistry

authors： Cook JH,Pratt RS,Lilien J

更新日期：1984-02-28 00:00:00

abstract：:Microfibrils having a diameter of 10-12 nm, found either in association with elastin or independently, are an important component of the extracellular matrix of many tissues. Because isolation of native proteins composing the microfibrils has proven difficult, information on structure/function relationships is limited...

journal_title：Biochemistry

authors： Bashir MM,Abrams WR,Rosenbloom J,Kucich U,Bacarra M,Han MD,Brown-Augsberger P,Mecham R,Rosenbloom J

更新日期：1994-01-18 00:00:00

abstract：:Amyloid fibrils have been classically defined as linear, nonbranched polymeric proteins with a cross beta-sheet structure and the ability to alter the optical properties of the amyloid-specific dye Congo Red. Mounting evidence suggests that soluble oligomeric peptide assemblies approximately 2-20 nm in diameter are cr...

journal_title：Biochemistry

authors： Moore RA,Hayes SF,Fischer ER,Priola SA

更新日期：2007-06-19 00:00:00

abstract：:To investigate the effect of a reduced level of selenocysteine (Sec) tRNA[Ser]Sec in selenoprotein biosynthesis, two mouse embryonic stem (ES) cell lines heterozygous for the corresponding gene were generated by homologous recombination of the host genome with targeting vectors encoding a deleted or a disrupted tRNA[S...

journal_title：Biochemistry

authors： Chittum HS,Baek HJ,Diamond AM,Fernandez-Salguero P,Gonzalez F,Ohama T,Hatfield DL,Kuehn M,Lee BJ

更新日期：1997-07-15 00:00:00

abstract：:The baker's yeast saccharopine dehydrogenase (EC 1.5.1.7) was inactivated by 2,3-butanedione following pseudo-first-order reaction kinetics. The pseudo-first-order rate constant for inactivation was linearly related to the butanedione concentration, and a value of 7.5 M-1 min-1 was obtained for the second-order rate c...

journal_title：Biochemistry

authors： Fujioka M,Takata Y

更新日期：1981-02-03 00:00:00

abstract：:Nitrosylhemoglobin (HbFe(II)NO) has been shown to be generated in vivo from the reaction of deoxyHb with NO(*) as well as with nitrite. Despite the physiological importance attributed to this form of Hb, its reactivity has not been investigated in detail. In this study, we showed that the rate of oxidation of HbFe(II)...

journal_title：Biochemistry

authors： Herold S,Röck G

更新日期：2005-04-26 00:00:00

abstract：:Fully functional variable fragments (Fv) of D1.3, a mouse antibody directed against the hen egg lysozyme, were readily produced as hybrids (Fv-MalE) with the maltose-binding protein of Escherichia coli and purified independently of their antigen-binding properties. We used site-directed mutations of residues in the co...

journal_title：Biochemistry

authors： England P,Brégégère F,Bedouelle H

更新日期：1997-01-07 00:00:00

abstract：:We have measured the frequency of the carbon-hydrogen stretching mode of the pro-R and pro-S C4-H bonds of NADH in solution and when bound to pig heart lactate (LDH) or mitochondrial malate (mMDH) dehydrogenases. This is achieved by specifically deuterating the C4 pro-R or pro-S hydrogens of NADH and determining the f...

journal_title：Biochemistry

authors： Deng H,Zheng J,Sloan D,Burgner J,Callender R

更新日期：1992-06-02 00:00:00

abstract：:The full-length cDNA encoding a novel human intracellular serine proteinase inhibitor has been sequenced and found to encode a 376 amino acid protein (M(r) approximately 42.5K) that we designate as cytoplasmic antiproteinase. Analysis of the primary structure revealed that the cytoplasmic antiproteinase has the majori...

journal_title：Biochemistry

authors： Morgenstern KA,Sprecher C,Holth L,Foster D,Grant FJ,Ching A,Kisiel W

更新日期：1994-03-22 00:00:00

abstract：:Effects of eight differently substituted 1,4-benzoquinones (BQs) on the quantum yield of photoactivation of oxygen evolution (reconstitution of the Mn cluster) were examined with wheat photosystem II (PSII) membranes depleted of the Mn cluster by treatment with 1.0 mM NH2OH. Illumination with 10 flashes at 0.25-s inte...

journal_title：Biochemistry

authors： Miyao-Tokutomi M,Inoue Y

更新日期：1992-01-21 00:00:00

abstract：:The circular dichroism (CD) of Pfl filamentous virus has been examined over the temperature range 0-40 degrees C, in the absence and presence of Hg(II), Ag(I), and Cu(II). Thermal difference CD spectra were obtained by subtraction of spectra recorded above and below a thermally induced structure transition near 12 deg...

journal_title：Biochemistry

authors： Casadevall A,Day LA

更新日期：1988-05-17 00:00:00

abstract：:Comparisons of membrane glycopeptides from baby hamster kidney fibroblasts (BHK21/C13) and a clone transformed by Rous sarcoma virus (C13/B4) were made by using cells metabolically labeled with radioactive D-glucose and L-fucose. Most of the glycopeptides were metabolically labeled with both the general and the specif...

journal_title：Biochemistry

authors： Glick MC

更新日期：1979-06-12 00:00:00

abstract：:The beta(2) subunit of a class Ia or Ib ribonucleotide reductase (RNR) is activated when its carboxylate-bridged Fe(2)(II/II) cluster reacts with O(2) to oxidize a nearby tyrosine (Y) residue to a stable radical (Y(*)). During turnover, the Y(*) in beta(2) is thought to reversibly oxidize a cysteine (C) in the alpha(2...

journal_title：Biochemistry

authors： Jiang W,Yun D,Saleh L,Bollinger JM Jr,Krebs C

更新日期：2008-12-30 00:00:00

abstract：:Dihydrofolate reductase (DHFR) is often used as a model system to study the relation between protein dynamics and catalysis. We have studied a number of variants of the cold-adapted DHFR from Moritella profunda (MpDHFR), in which the catalytically important M20 and FG loops have been altered, and present a comparison ...

journal_title：Biochemistry

authors： Behiry EM,Evans RM,Guo J,Loveridge EJ,Allemann RK

更新日期：2014-07-29 00:00:00

abstract：:High-affinity mu-conotoxin block of skeletal muscle Na+ channels depends on an arginine at position 13 (Arg-13). To understand both the mechanism of toxin interaction and the general structure of its binding site in the channel mouth, we examined by thermodynamic mutant cycle analysis the interaction between the criti...

journal_title：Biochemistry

authors： Chang NS,French RJ,Lipkind GM,Fozzard HA,Dudley S Jr

更新日期：1998-03-31 00:00:00

abstract：:The structure of a 22 amino acid peptide, TPPI [Nedved, M. L., Gottlieb, P. A., & Moe, G. R. (1994) Nucleic Acids Res. 22, 5024-5030], that is similar to the proline repeat segment of the replication arrest protein, Tus, has been determined by 1H NMR in 50% trifluroethanol. The structure is a novel left-handed helix h...

journal_title：Biochemistry

authors： Butcher DJ,Nedved ML,Neiss TG,Moe GR

更新日期：1996-01-23 00:00:00

abstract：:The backbone dynamics of uniformly 15N-labeled interleukin-1 beta are investigated by using two-dimensional inverse detected heteronuclear 15N-1H NMR spectroscopy. 15N T1, T2, and NOE data at a spectrometer frequency of 600 MHz are obtained for 90% of the backbone amide groups. The data provide evidence for motions on...

journal_title：Biochemistry

authors： Clore GM,Driscoll PC,Wingfield PT,Gronenborn AM

更新日期：1990-08-14 00:00:00

abstract：:The crystal structure of a C-terminal domain of enterohemorrhagic Escherichia coli type III effector NleH2 has been determined to 2.6 Å resolution. The structure resembles those of protein kinases featuring the catalytic, activation, and glycine-rich loop motifs and ATP-binding site. The position of helix αC and the l...

journal_title：Biochemistry

authors： Halavaty AS,Anderson SM,Wawrzak Z,Kudritska M,Skarina T,Anderson WF,Savchenko A

更新日期：2014-04-22 00:00:00

abstract：:A mechanistic study of rat liver alpha-(2-->6) sialyltransferase (ST) is presented that includes isotope trapping experiments and kinetic isotope effects on V/K for the ST-catalyzed reaction of isotopically labeled CMP-N-acetylneuraminate and N-acetyllactosamine. The isotope trapping experiments confirmed that the kin...

journal_title：Biochemistry

authors： Bruner M,Horenstein BA

更新日期：1998-01-06 00:00:00

abstract：:Prion proteins (PrP) are the infectious agent in transmissible spongiform encephalopathies (i.e., mad cow disease). To be infectious, prion proteins must undergo a conformational change involving a decrease in α-helical content along with an increase in β-strand content. This conformational change was evaluated by mea...

journal_title：Biochemistry

authors： Samson AO,Levitt M

更新日期：2011-03-29 00:00:00

abstract：:The dissociation of the trypsin-sensitive basic tails of the core histones in core chromatin has been followed as a function of [NaCl] using proton NMR spectroscopy. The tails dissociate in a highly cooperative all or none manner over the salt concentration range 0.2-0.6 M, that is, below the salt concentration requir...

journal_title：Biochemistry

authors： Walker IO

更新日期：1984-11-06 00:00:00

abstract：:Extracellular matrix (ECM) proteins, such as collagen and fibronectin, play vital roles in development and maintenance of hard tissue (bone or tooth) and are, consequently, thoroughly investigated for construction of biomimetic scaffolds in combination with calcium phosphate (CaP) material (the major component of hard...

journal_title：Biochemistry

authors： Chowdhury EH,Nagaoka M,Ogiwara K,Zohra FT,Kutsuzawa K,Tada S,Kitamura C,Akaike T

更新日期：2005-09-20 00:00:00

abstract：:Amyloid beta (Abeta) peptide amyloidogenesis, involving the formation of numerous distinct quaternary structures, appears to cause Alzheimer's disease. However, the precise identification of the toxic structure(s) and the neurotoxicity mechanism(s) remains elusive. Mutating the Abeta 1-40 Phe19-Phe20 backbone amide bo...

journal_title：Biochemistry

authors： Bieschke J,Siegel SJ,Fu Y,Kelly JW

更新日期：2008-01-08 00:00:00