Isotope trapping and kinetic isotope effect studies of rat liver alpha-(2-->6)-sialyltransferase.

abstract：:Previous optical and electron paramagnetic resonance (EPR) spectroscopic studies of the newly discovered peroxynitrite scavenging pseudoperoxidase from Leishmania major (LmPP) suggested that ferric LmPP contained a six-coordinate low-spin (6cLS) heme with a thiolate ligand, presumably a cysteine, bound to its heme iro...

journal_title：Biochemistry

authors： Saha R,Bose M,Sen Santara S,Roy J,Adak S

更新日期：2013-12-10 00:00:00

abstract：:Two mutant versions of Escherichia coli aspartate transcarbamylase were created by site-specific mutagenesis. Arg-234 of the 240s loop was replaced by serine in order to help deduce the function of the interactions that normally occur between Arg-234 and both Glu-50 and Gln-231 in the R state of the enzyme. The other ...

journal_title：Biochemistry

authors： Middleton SA,Kantrowitz ER

更新日期：1988-11-15 00:00:00

abstract：:The Autographa californica multinucleocapsid nuclear polyhedrosis virus genome contains nine homologous region (hr1, hr1a, hr2, hr2a, hr3, hr4a, hr4b, hr4c, and hr5) sequences that are thought to be involved in viral replication and activation of transcription. Our results show that the 750 bp hr1 sequence is capable ...

journal_title：Biochemistry

authors： Venkaiah B,Viswanathan P,Habib S,Hasnain SE

更新日期：2004-06-29 00:00:00

abstract：:Oligonucleotides labeled with hexachlorofluorescein (hex) have enabled the interaction of the restriction endonuclease EcoRV with DNA to be evaluated using fluorescence anisotropy. The sensitivity of hex allowed measurements at oligonucleotide concentrations as low as 1 nM, enabling K(D) values in the low nanomolar ra...

journal_title：Biochemistry

authors： Reid SL,Parry D,Liu HH,Connolly BA

更新日期：2001-02-27 00:00:00

abstract：:Thermodynamics of 39 oligonucleotides with internal G.T mismatches dissolved in 1 M NaCl were determined from UV absorbance versus temperature profiles. These data were combined with literature values of six sequences to derive parameters for 10 linearly independent trimer and tetramer sequences with G.T mismatches an...

journal_title：Biochemistry

authors： Allawi HT,SantaLucia J Jr

更新日期：1997-08-26 00:00:00

abstract：:The crystal structure of a C-terminal domain of enterohemorrhagic Escherichia coli type III effector NleH2 has been determined to 2.6 Å resolution. The structure resembles those of protein kinases featuring the catalytic, activation, and glycine-rich loop motifs and ATP-binding site. The position of helix αC and the l...

journal_title：Biochemistry

authors： Halavaty AS,Anderson SM,Wawrzak Z,Kudritska M,Skarina T,Anderson WF,Savchenko A

更新日期：2014-04-22 00:00:00

abstract：:Quasi-elastic laser light scattering is used to examine the cholera toxin mediated agglutination of ganglioside GM1 containing phospholipid vesicles and Gm1-coated polystyrene spheres. We find that the ability of cholera toxin to agglutinate Gm1-containing phospholipid vesicles depends markedly on the lipid compositio...

journal_title：Biochemistry

authors： Dwyer JD,Bloomfield VA

更新日期：1982-06-22 00:00:00

abstract：:The gene for human pancreatic cholesterol esterase consists of 11 exons and 10 introns and is 9.2 kb in length. The last and longest exon (841 nucleotides) is unique to the human gene. Functional amino acids are encoded on separate exons. The leader sequence is encoded by a single exon which carries two additional N-t...

journal_title：Biochemistry

authors： Kumar BV,Aleman-Gomez JA,Colwell N,Lopez-Candales A,Bosner MS,Spilburg CA,Lowe M,Lange LG

更新日期：1992-07-07 00:00:00

abstract：:Factors determining conformations of imidazole axially coordinated to heme in heme proteins were investigated by analyzing 693 hemes in 432 different crystal structures of heme proteins from the Protein Data Bank (PDB), where at least one histidine is ligated to heme. The results from a search of the PDB for protein s...

journal_title：Biochemistry

authors： Zarić SD,Popović DM,Knapp EW

更新日期：2001-07-03 00:00:00

abstract：:Structures of several voltage-gated sodium (NaV) channels from bacteria have been determined recently, but the same feat might not be achieved for the mammalian counterparts in the near future. Thus, at present, computational studies of the mammalian NaV channels have to be performed using homology models based on the...

journal_title：Biochemistry

authors： Patel D,Mahdavi S,Kuyucak S

更新日期：2016-03-29 00:00:00

abstract：:An amino-terminal deletion mutant (residues 1-43) of human apolipoprotein A-I (apo hA-I) has been produced from a bacterial expression system to explore the structural and functional role of these amino acids, encoded by exon 3, in apo hA-I. Lipid binding of apo delta (1-43)A-I and lipid binding of apo hA-I are very s...

journal_title：Biochemistry

authors： Rogers DP,Brouillette CG,Engler JA,Tendian SW,Roberts L,Mishra VK,Anantharamaiah GM,Lund-Katz S,Phillips MC,Ray MJ

更新日期：1997-01-14 00:00:00

abstract：:The mitochondrial tyrosyl tRNA synthetase from Neurospora crassa (CYT-18 protein) is a bifunctional group I intron splicing cofactor. CYT-18 is capable of splicing multiple group I introns from a wide variety of sources by stabilizing the catalytically active intron structures. CYT-18 and mt TyrRSs from related fungal...

journal_title：Biochemistry

authors： Geng C,Paukstelis PJ

更新日期：2014-03-04 00:00:00

abstract：:Photochemical and subsequent thermal reactions of rhodopsin containing 9-cis-retinal [Rh(9)] or one of four analogues with 9-cis geometries formed from ring-modified retinals, alpha-retinal [alpha Rh(9)], acyclic retinal [AcRh(9)], acyclic alpha-retinal [Ac alpha Rh(9)], and 5-isopropyl-alpha-retinal [P alpha Rh(9)] w...

journal_title：Biochemistry

authors： Okada T,Kandori H,Shichida Y,Yoshizawa T,Denny M,Zhang BW,Asato AE,Liu RS

更新日期：1991-05-14 00:00:00

abstract：:Reticulocyte lysate contains a chaperone system that assembles glucocorticoid receptor (GR).hsp90 heterocomplexes. Using purified proteins, we have prepared a five-protein heterocomplex assembly system consisting of two proteins essential for heterocomplex assembly-hsp90 and hsp70-and three proteins that act as co-cha...

journal_title：Biochemistry

authors： Kanelakis KC,Murphy PJ,Galigniana MD,Morishima Y,Takayama S,Reed JC,Toft DO,Pratt WB

更新日期：2000-11-21 00:00:00

abstract：:Acyl carrier protein (ACP) is the central player in fatty acid (FA) biosynthesis. It covalently binds all FA intermediates and presents them to the enzymes needed for elongation. Bacterial ACP must interact with a large number of proteins, which raises the question of how different acyl-ACPs are recognized and disting...

journal_title：Biochemistry

authors： Chan DI,Tieleman DP,Vogel HJ

更新日期：2010-04-06 00:00:00

abstract：:The amino acid sequence of the sex steroid binding protein (SBP) from human plasma has been determined. The SBP subunit consists of a 373-residue polypeptide chain containing two disulfide bonds and three oligosaccharide chains. The sequence was solved primarily by analysis of peptides derived by cleavage at either ly...

journal_title：Biochemistry

authors： Walsh KA,Titani K,Takio K,Kumar S,Hayes R,Petra PH

更新日期：1986-11-18 00:00:00

abstract：:Deletion of Glu146, Thr147, and Trp148 from thrombin dramatically alters its interactions with substrates, ligands, and inhibitors, and the changes appear to result from nonlocal modification of thrombin's structure [Le Bonniec, B. F., Guinto, E. R., & Esmon, C. T. (1992) J. Biol. Chem. 267, 19341-19348]. In an attemp...

journal_title：Biochemistry

authors： Le Bonniec BF,Betz A,Guinto ER,Esmon CT,Stone SR

更新日期：1994-04-05 00:00:00

abstract：:GGAs are a family of vesicle-coating regulatory proteins that function in intracellular protein transport. A GGA molecule contains four domains, each mediating interaction with other proteins in carrying out intracellular transport. The GAT domain of GGAs has been identified as the structural entity that binds membran...

journal_title：Biochemistry

authors： Zhu G,Zhai P,He X,Terzyan S,Zhang R,Joachimiak A,Tang J,Zhang XC

更新日期：2003-06-03 00:00:00

abstract：:A highly active, large-scale preparation of ubiquinol:cytochrome c2 oxidoreductase (EC 1.10.2.2; cytochrome bc1 complex) has been obtained from Rhodobacter sphaeroides. The enzyme was solubilized from chromatophores by using dodecyl maltoside in the presence of glycerol and was purified by anion-exchange and gel filtr...

journal_title：Biochemistry

authors： Andrews KM,Crofts AR,Gennis RB

更新日期：1990-03-20 00:00:00

abstract：:Cyclopentenone prostanoids (cyP) arise as important modulators of inflammation and cell proliferation. Although their physiological significance has not been fully elucidated, their potent biological effects have spurred their study as leads for the development of therapeutic agents. A key determinant of cyP action is...

journal_title：Biochemistry

authors： Renedo M,Gayarre J,García-Domínguez CA,Pérez-Rodríguez A,Prieto A,Cañada FJ,Rojas JM,Pérez-Sala D

更新日期：2007-06-05 00:00:00

abstract：:Annexin A2 is a member of the annexin family of Ca(2+)-dependent lipid binding proteins and believed to be engaged in membrane transport processes in a number of cell types. In small intestinal enterocytes, we localized annexin A2 to the brush border region, where it was found mainly on the lumenal side of the microvi...

journal_title：Biochemistry

authors： Danielsen EM,van Deurs B,Hansen GH

更新日期：2003-12-16 00:00:00

abstract：:In an earlier study, β³-puromycin was used for the selection of modified ribosomes, which were utilized for the incorporation of five different β-amino acids into Escherichia coli dihydrofolate reductase (DHFR). The selected ribosomes were able to incorporate structurally disparate β-amino acids into DHFR, in spite of...

journal_title：Biochemistry

authors： Maini R,Chowdhury SR,Dedkova LM,Roy B,Daskalova SM,Paul R,Chen S,Hecht SM

更新日期：2015-06-16 00:00:00

abstract：:The interaction between eosin-5-maleimide (EMA), an inhibitor of the anion transport protein, band 3, and I-, a transportable substrate, was investigated by fluorescence quenching. The Stern-Volmer plot for the quenching reaction between EMA-labeled band 3 and I- exhibits downward curvature both in human erythrocyte g...

journal_title：Biochemistry

authors： Pan RJ,Cherry RJ

更新日期：1995-04-11 00:00:00

abstract：:We have measured the concentration of mRNAs coding for immunoglobulins, k and lambda type light chains and gamma 1 type heavy chain, in mouse spleen cells activated by bacterial lipopolysaccharide or sheep red blood cells. These mRNAs were quantitated by hybridization to radioactive DNA complementary to highly purifie...

journal_title：Biochemistry

authors： Honjo T,Shimizu A,Tsuda M,Natori S,Katatoka T,Dohmoto C,Mano Y

更新日期：1977-12-27 00:00:00

abstract：:A study of the voltage induction of transient pores in phospholipid bilayer vesicles is reported. Unilamellar vesicles (dipalmitoylphosphatidylcholine), with a size distribution of 100 +/- 30 nm, were prepared by the method of Enoch & Strittmatter [Enoch, H., & Strittmatter, P. (1979) Proc. Natl. Acad. Sci. U.S.A. 76,...

journal_title：Biochemistry

authors： Teissie J,Tsong TY

更新日期：1981-03-17 00:00:00

abstract：:β-Lactamase inhibitors (BLIs) restore the efficacy of otherwise obsolete β-lactams. However, commercially available BLIs are not effective against metallo-β-lactamases (MBLs), which continue to be disseminated globally. One group of the most clinically important MBLs is the VIM family. The discovery of VIM-24, a natur...

journal_title：Biochemistry

authors： Mojica MF,Mahler SG,Bethel CR,Taracila MA,Kosmopoulou M,Papp-Wallace KM,Llarrull LI,Wilson BM,Marshall SH,Wallace CJ,Villegas MV,Harris ME,Vila AJ,Spencer J,Bonomo RA

更新日期：2015-05-26 00:00:00

abstract：:1-Aminocyclopropanephosphonate (ACPP) was synthesized, and its effects on the pyridoxal 5'-phosphate linked enzymes 1-aminocyclopropanecarboxylate (ACPC) deaminase from Pseudomonas sp. ACPC and alanine racemase from Bacillus stearothermophilus were studied. ACPP was found to be a potent inhibitor of both enzymes with ...

journal_title：Biochemistry

authors： Erion MD,Walsh CT

更新日期：1987-06-16 00:00:00

abstract：:The integral membrane protein diacylglycerol kinase (DGK) from Escherichia coli has been reversibly unfolded in a protein/detergent/mixed micelle system by varying the molar ratio of n-decyl beta-D-maltoside (DM) and sodium dodecyl sulfate (SDS). Unfolding was monitored by circular dichroism (CD) and ultraviolet (UV) ...

journal_title：Biochemistry

authors： Lau FW,Bowie JU

更新日期：1997-05-13 00:00:00

abstract：:The three-dimensional chalice-like crystal structure of initiation factor 2 IF2/eIF5B from Methanobacterium thermoautotrophicum represents a novel fold and domain architecture in which the N-terminal G domain and the C-terminal C domain are separated by an approximately 40 A alpha-helix. Homologous Thermus thermophilu...

journal_title：Biochemistry

authors： Zoldák G,Sedlák E,Wolfrum A,Musatov A,Fedunová D,Szkaradkiewicz K,Sprinzl M

更新日期：2008-04-29 00:00:00

abstract：:We have analyzed the role of several protein motifs in controlling the membrane association of the xlcaax-1 protein in Xenopus oocytes. Xlcaax-1 is a maternally expressed protein that during development is associated with the basal lateral membrane of polarized epithelial cells. It is enriched in the tubule cells of t...

journal_title：Biochemistry

authors： Kloc M,Li XX,Etkin LD

更新日期：1993-08-17 00:00:00