Two upstream cysteines and the CAAX motif but not the polybasic domain are required for membrane association of Xlcaax in Xenopus oocytes.

abstract：:F-STOP is a microtubule-associated protein that stabilizes microtubules in a calmodulin (CaM)-dependent manner. All members of the stable tubule only polypeptide (STOP) family have a central domain that contains nearly identical multiple repeats, and a CaM binding motif is present in multiple copies within this domain...

journal_title：Biochemistry

authors： Bouvier D,Vanhaverbeke C,Simorre JP,Arlaud GJ,Bally I,Forge V,Margolis RL,Gans P,Kleman JP

更新日期：2003-10-07 00:00:00

abstract：:The interactions of a series of spin-labeled fatty acids, in which the nitroxide ring is incorporated in different ways as an integral part of the hydrocarbon chain, with the (Na+,K+)-ATPase in membranes from Squalus acanthias, have been studied by electron spin resonance spectroscopy. The fatty acids are 2,4-, 2,5-, ...

journal_title：Biochemistry

authors： Esmann M,Hideg K,Marsh D

更新日期：1988-05-31 00:00:00

abstract：:The selectivity of binding of Escherichia coli RNA polymerase holoenzyme to a promoter-containing fragment of T7 DNA has been investigated over a range of solution conditions by using a double-label nitrocellulose filter binding assay. A 32P-labeled HaeIII restriction fragment of T7 D111 DNA containing the A1 and D pr...

journal_title：Biochemistry

authors： Strauss HS,Burgess RR,Record MT Jr

更新日期：1980-07-22 00:00:00

abstract：:Various omega-halogenated carboxy acids and amides were evaluated as potential active-site-directed reagents for alcohol dehydrogenase. 2-Bromoacetamide and bromoacetic and 3-bromopropionic acids inactivated the enzyme; AMP, NAD+, and NADH markedly decreased the rate of inactivation. Some omega-halogenated carboxyamid...

journal_title：Biochemistry

authors： Fries RW,Bohlken DP,Blakley RT,Plapp BV

更新日期：1975-11-18 00:00:00

abstract：:Wideline 2H NMR spectroscopy was used to assess the conformational and orientational effects of N-acetylneuraminic acid (NeuAc) (sialic acid) as a component of a particular oligosaccharide chain at a bilayer membrane surface. For this purpose, three glycosphingolipids, sharing a neutral core tetrasaccharide and differ...

journal_title：Biochemistry

authors： Jones DH,Barber KR,Grant CW

更新日期：1996-04-16 00:00:00

abstract：:Glu58 is known to participate in phosphodiester transesterification catalyzed by the enzyme RNase T1. For Glu58 RNase T1, an altered mechanism has been proposed in which His40 replaces Glu58 as the base catalyst [Steyaert, J., Hallenga, K., Wyns, L., & Stanssens, P. (1990) Biochemistry 29, 9064-9072]. Glu58Ala Rnase T...

journal_title：Biochemistry

authors： Pletinckx J,Steyaert J,Zegers I,Choe HW,Heinemann U,Wyns L

更新日期：1994-02-22 00:00:00

abstract：:Despite the high degree of sequence similarity in their basic-helix-loop-helix (BHLH) domains, MASH-1 and MyoD are involved in different biological processes. In order to define possible differences between the DNA binding specificities of these two proteins, we investigated the DNA binding properties of MASH-1 by cir...

journal_title：Biochemistry

authors： Meierhan D,el-Ariss C,Neuenschwander M,Sieber M,Stackhouse JF,Allemann RK

更新日期：1995-09-05 00:00:00

abstract：:Phosphorylated proteins may play an important role in regulating the metabolism or function of rod photoreceptors. In mammalian retinas, a photoreceptor protein of 33 000 (33K) molecular weight is phosphorylated in a cyclic nucleotide dependent manner in vitro. Since light initiates the activation of a photoreceptor-s...

journal_title：Biochemistry

authors： Lee RH,Brown BM,Lolley RN

更新日期：1984-04-24 00:00:00

abstract：:We recently showed that ligand-mediated cross-linking of FcepsilonRI, the high-affinity receptor for immunoglobulin E, on RBL-2H3 mast cells results in its co-isolation with detergent-resistant membranes (DRM) and its consequent tyrosine phosphorylation by the co-localized tyrosine kinase Lyn that is a critical early ...

journal_title：Biochemistry

authors： Fridriksson EK,Shipkova PA,Sheets ED,Holowka D,Baird B,McLafferty FW

更新日期：1999-06-22 00:00:00

abstract：:The vitamin K epoxide reductase (VKORC1) enzyme is of primary importance in many physiological processes, i.e., blood coagulation, energy metabolism, and arterial calcification prevention, due to its role in the vitamin K cycle. Indeed, VKORC1 catalyzes reduction of vitamin K epoxide to quinone and then to hydroquinon...

journal_title：Biochemistry

authors： Chatron N,Abi Khalil R,Benoit E,Lattard V

更新日期：2020-04-07 00:00:00

abstract：:Hydrophobic interactions are responsible for stabilizing leucine zippers in peptides containing heptad repeats. The effects of substituting leucine by phenylalanine and alanine by glycine on the self-assembly of coiled-coils were examined in minimalist antimicrobial peptides designed to form amphipathic alpha-helices....

journal_title：Biochemistry

authors： Javadpour MM,Barkley MD

更新日期：1997-08-05 00:00:00

abstract：:The three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor (RBI) from seeds of ragi (Eleusine coracana Gaertneri) has been determined in solution using multidimensional 1H and 15N NMR spectroscopy. The inhibitor consists of 122 amino acids, with 5 disulfide bridges, and belongs to the plant al...

journal_title：Biochemistry

authors： Strobl S,Mühlhahn P,Bernstein R,Wiltscheck R,Maskos K,Wunderlich M,Huber R,Glockshuber R,Holak TA

更新日期：1995-07-04 00:00:00

abstract：:Heparin lyases are valuable tools for generating oligosaccharide fragments and in sequence determination of heparan sulfate (HS). Heparin lyase III is known to cleave the linkages between N-acetylglucosamine (GlcNAc) or N-sulfated glucosamine (GlcNS) and glucuronic acid (GlcA) as the primary sites and the linkages bet...

journal_title：Biochemistry

authors： Chai W,Leteux C,Westling C,Lindahl U,Feizi T

更新日期：2004-07-06 00:00:00

abstract：:An equimolar mixture of DL-[alpha-2H]- and DL-[alpha-13C]mandelate, when incubated with mandelate racemase (EC 5.1.2.2), shows conversion of singly labeled mandelate to unlabeled mandelate, due to solvent exchange of the alpha proton, while the level of doubly labeled mandelate remains at a constant low level. Similar...

journal_title：Biochemistry

authors： Sharp TR,Hegeman GD,Kenyon GL

更新日期：1977-03-22 00:00:00

abstract：:The dimeric nature of the HIV protease has been exploited to devise a novel mode of inhibiting the enzyme. The use of defective monomers or nonidentical subunits to exchange with wild-type homodimers produces catalytically defective heterodimers. Incubation of the HIV1 or HIV2 protease with a 4-fold molar excess of an...

journal_title：Biochemistry

authors： Babé LM,Pichuantes S,Craik CS

更新日期：1991-01-08 00:00:00

abstract：:The catalytic and structural properties of divalent metal ion cofactor binding sites in the dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) from Haemophilus influenzae were investigated. Co(II)-substituted DapE enzyme was 25% more active than the Zn(II)-loaded form of the enzyme. Interestingly, Mn...

journal_title：Biochemistry

authors： Bienvenue DL,Gilner DM,Davis RS,Bennett B,Holz RC

更新日期：2003-09-16 00:00:00

abstract：:The reaction of nitric oxide (NO) with fast cytochrome bo from Escherichia coli has been studied by electronic absorption, MCD, and EPR spectroscopy. Titration of the enzyme with NO showed the formation of two distinct species, consistent with NO binding stoichiometries of 1:1 and 2:1 with observed dissociation consta...

journal_title：Biochemistry

authors： Butler CS,Seward HE,Greenwood C,Thomson AJ

更新日期：1997-12-23 00:00:00

abstract：:Membrane-bound phosphodiesterase 6 (PDE6) plays an important role in visual signal transduction by regulating cGMP levels in rod photoreceptor cells. Our understanding of PDE6 catalysis and structure suffers from inadequate characterization of the α and β subunit catalytic core, interactions of the core with two intri...

journal_title：Biochemistry

authors： Baker BY,Palczewski K

更新日期：2011-11-08 00:00:00

abstract：:Fluorescence recovery after photobleaching was used to measure the diffusion coefficient of green fluorescent protein (GFP, 27 kDa) in Escherichia coli in the presence or absence of four coexpressed proteins: cytoplasmic maltose binding protein (42 kDa), tau-40 (45 kDa), alpha-synuclein (14 kDa), or calmodulin (17 kDa...

journal_title：Biochemistry

authors： Slade KM,Baker R,Chua M,Thompson NL,Pielak GJ

更新日期：2009-06-16 00:00:00

abstract：:Here we report that the Methanocaldococcus jannaschii enzyme derived from the MJ0309 gene is an Fe(II) dependent agmatinase (SpeB). This is the first report of an iron-dependent agmatinase. We demonstrate that aerobically isolated recombinant enzyme contains two disulfide bonds and only a trace amount of any metal and...

journal_title：Biochemistry

authors： Miller D,Xu H,White RH

更新日期：2012-04-10 00:00:00

abstract：:Subunit II of the aa3 type cytochrome c oxidase contains a binuclear copper center (CuA) which functions as the entry point for electrons donated by cytochrome c. We have introduced site-specific mutations in residues liganding the CuA center in the oxidase of the bacterium Paracoccus denitrificans; the purified, full...

journal_title：Biochemistry

authors： Zickermann V,Wittershagen A,Kolbesen BO,Ludwig B

更新日期：1997-03-18 00:00:00

abstract：:The importance of the fully conserved active site proline, Pro168, for the reaction mechanism of triosephosphate isomerase (TIM) has been investigated by studying the enzymatic and crystallographic properties of the P168A variant of trypanosomal TIM. In TIM, Pro168 follows the key catalytic residue Glu167, situated at...

journal_title：Biochemistry

authors： Casteleijn MG,Alahuhta M,Groebel K,El-Sayed I,Augustyns K,Lambeir AM,Neubauer P,Wierenga RK

更新日期：2006-12-26 00:00:00

abstract：:Electron donation from the soluble cytochrome (cyt) c2 to the photooxidized primary donor, P+, of reaction centers isolated from Rhodobacter sphaeroides was studied by using chemical zero-length cross-linking. This cross-linking stabilizes a 1:1 covalent complex between subunit M of the reaction center and cyt c2. In ...

journal_title：Biochemistry

authors： Drepper F,Dorlet P,Mathis P

更新日期：1997-02-11 00:00:00

abstract：:Previous studies have shown that the short-motif electroplax Na channel is phosphorylated in vitro by cyclic AMP-dependent protein kinase (PKA) at serines 6 or 7 and 1776 and threonine 17 (Emerick & Agnew, 1989). We here show that phosphatase treatment of solubilized, purified Na channels enhanced subsequent PKA label...

journal_title：Biochemistry

authors： Emerick MC,Shenkel S,Agnew WS

更新日期：1993-09-14 00:00:00

abstract：:The red pigments in meat products, including cooked cured ham, arise from the reaction of myoglobin with nitric oxide generated from exogenous nitrite. Since carcinogenic nitrosoamines may be generated by the treatment of meats with nitrite, the production of nitrite-free meat products is an attractive alternative. Ra...

journal_title：Biochemistry

authors： Taketani S,Ishigaki M,Mizutani A,Uebayashi M,Numata M,Ohgari Y,Kitajima S

更新日期：2007-12-25 00:00:00

abstract：:A covalently linked actin dimer is identified in solutions of actin prepared from an acetone powder from skeletal muscle. This actin dimer acts as an actin nucleating factor (ANF), decreasing the half-time for spontaneous actin polymerization. ANF reacts with antibodies to both the N- and C-terminal portions of actin ...

journal_title：Biochemistry

authors： Selden LA,Kinosian HJ,Estes JE,Gershman LC

更新日期：2000-01-11 00:00:00

abstract：:The side chains of histidine and aspartate residues form a hydrogen bond in the active sites of many enzymes. In serine proteases, the His...Asp hydrogen bond of the catalytic triad is known to contribute greatly to catalysis, perhaps via the formation of a low-barrier hydrogen bond. In bovine pancreatic ribonuclease ...

journal_title：Biochemistry

authors： Schultz LW,Quirk DJ,Raines RT

更新日期：1998-06-23 00:00:00

abstract：:We have synthesized 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho(N-oleoyl)serine (N-acyl-PS) and 1,2-dioleoyl-sn-glycero-3-phospho-Tris (phosphatidyl-Tris) and have characterized both phospholipids by their chemical and chromatographic properties, as well as by their IR, 13C NMR, and 1H NMR spectra. Comparison of these d...

journal_title：Biochemistry

authors： Schmid PC,Kumar VV,Weis BK,Schmid HH

更新日期：1991-02-19 00:00:00

abstract：:The dark reaction of tris(hydroxymethyl)aminomethane (Tris) with the O2-evolving center of photosystem II (PSII) in the S1 state causes irreversible inhibition of O2 evolution. Similar inhibition is observed for several other amines: NH3, CH3NH2, (CH3)2NH, ethanolamine, and 2-amino-2-ethyl-1,3-propanediol. In PSII mem...

journal_title：Biochemistry

authors： Rickert KW,Sears J,Beck WF,Brudvig GW

更新日期：1991-08-13 00:00:00

abstract：:Monoclonal antibodies directed against epitopes on each of the five subunits (alpha, beta, gamma, delta, and epsilon) of the Escherichia coli F1 ATPase (ECF1) have been prepared and used to localize the subunits in the enzyme complex. Fab' fragments, prepared by pepsin digestion of the antibodies, were bound to ECF1 a...

journal_title：Biochemistry

authors： Gogol EP,Aggeler R,Sagermann M,Capaldi RA

更新日期：1989-05-30 00:00:00