Abstract：
:Fluorescence recovery after photobleaching was used to measure the diffusion coefficient of green fluorescent protein (GFP, 27 kDa) in Escherichia coli in the presence or absence of four coexpressed proteins: cytoplasmic maltose binding protein (42 kDa), tau-40 (45 kDa), alpha-synuclein (14 kDa), or calmodulin (17 kDa). The GFP diffusion coefficient remains constant regardless of the type of coexpresseed protein and whether or not the coexpressed protein was induced. We conclude that expression of these soluble proteins has little to no effect on the diffusion of GFP. These results have implications for the utility of in-cell nuclear magnetic resonance spectroscopy.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Slade KM,Baker R,Chua M,Thompson NL,Pielak GJdoi
10.1021/bi9004107subject
Has Abstractpub_date
2009-06-16 00:00:00pages
5083-9issue
23eissn
0006-2960issn
1520-4995journal_volume
48pub_type
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