Abstract：
:Mouse fibroblasts contain a macromolecular binding component (receptor) which binds glucocorticoids specifically and with high affinity. This study shows that there are three different cellular forms of bound receptor and that it is experimentally possible to markedly alter the subcellular distribution of these three forms. Cells incubated with (3H)triamcinolone acetonide were broken after hypotonic shock and a 7000g hypotonic supernatant was obtained; the pellet was extracted with 0.3 M KCl, yielding a nuclear extract; the remaining pellet was resuspended in water, sonicated, and assayed for "nuclear residual" (i.e., nonextractable) radioactivity. If whole cells are incubated at 0 degrees in a growth medium, almost all of the bound steroid is located in the hypotonic supernatant fraction. Incubation at 37 degrees produces a shift of the steroid-bound macromolecule into the nuclear extractable form, while omission of glucose and addition of KCN at 37 degrees markedly increase the nuclear residual form at the expense of both the nuclear-extractable and supernatant forms. Since DNase treatment of chromatin liberates a soluble steroid-receptor complex, we believe that the nuclear residual form may be steroid-receptor complex tightly bound to chromatin. We propose a model suggesting that an energy-requiring process is required to generate free receptor from the chromatin complex to complete the normal cellular recycling system.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Middlebrook JL,Wong MD,Ishii DN,Aronow Ldoi
10.1021/bi00672a030subject
Has Abstractpub_date
1975-01-14 00:00:00pages
180-6issue
1eissn
0006-2960issn
1520-4995journal_volume
14pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Monomeric sarcosine oxidase (MSOX) binds the L-proline zwitterion (pKa = 10.6). The reactive substrate anion is generated by ionization of the ES complex (pKa = 8.0). Tyr317 was mutated to Phe to determine whether this step might involve proton transfer to an active site base. The mutation does not eliminate the ioniz...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051898d
更新日期：2005-12-27 00:00:00
abstract：:Illumination of envelope vesicles prepared from Halobacterium halobium cells causes translocation of protons from inside to outside, due to the light-induced cycling of bacteriorhodopsin. This process results in a pH gradient across the membranes, an electrical potential, and the movements of K+ and Na+. The electrica...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00655a017
更新日期：1976-05-18 00:00:00
abstract：:Two synthetic peptides corresponding to sequences in HIV-1LAI gp41, (aa558-595) and T20 (aa 643-678), are strong inhibitors of HIV-1 viral fusion, having EC50 values of 1 microgram/mL and 1 ng/mL, respectively. Previous work suggested that T21 forms a coiled-coil structure in PBS solution, while T20 is primarily nonhe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9606962
更新日期：1996-10-22 00:00:00
abstract：:The D-alanyl-D-alanine-adding enzyme encoded by the murF gene catalyzes the ATP-dependent formation of UDP-N-acetylmuramyl-L-gamma-D-Glu-meso-diaminopimelyl-D-Ala-D-Ala (UDP-MurNAc-tripeptide). MurF has been cloned from Escherichia coli and expressed as a glutathione S-transferase (GST) fusion using the tac promoter-b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961872+
更新日期：1996-12-17 00:00:00
abstract：:Illuminating Ca2+-depleted photosystem (PS) II membranes generated two new EPR signals at g = 11 and 15 by perpendicular and parallel polarization modes, respectively. Two turnovers of the oxygen evolving center (OEC) beyond the modified S2' state are required for the appearance of these signals. The formation of the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972471c
更新日期：1998-03-03 00:00:00
abstract：:Cesium-133 NMR studies have been carried out on suspended human erythrocytes and on perfused rat hearts in media containing CsCl. The resulting spectra exhibit two sharp resonances, arising from intra- and extracellular Cs+, separated in chemical shift by 1.0-1.4 ppm. Thus, intra- and extracellular resonances are easi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00410a003
更新日期：1988-05-17 00:00:00
abstract：:Postsynaptic polypeptide neurotoxins isolated from the venoms of elapid and hydrophid snakes exhibit the ability to bind selectively to and inhibit different types of receptors that function in nerve signal transmission. On the basis of their amino acid sequences and three-dimensional structures, these neurotoxins are...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00179a011
更新日期：1994-04-05 00:00:00
abstract：:Temporins are short (10-13 amino acids) and linear antimicrobial peptides first isolated from the skin of the European red frog, Rana temporaria, and are effective against Gram-positive bacteria and Candida albicans. To get insight into their mechanism(s) of action, we compared the effects on model membranes exerted b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi011929e
更新日期：2002-04-02 00:00:00
abstract：:Thioredoxin reductase (TrxR) catalyzes the reduction of thioredoxin (Trx) by NADPH. A unique gene organization of TrxR and Trx has been found in Mycobacterium leprae, where TrxR and Trx are encoded by a single gene and, therefore, are expressed as a fusion protein (MlTrxR-Trx). This fusion enzyme is able to catalyze t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980754e
更新日期：1998-11-17 00:00:00
abstract：:Structure-function studies of antibody-antigen systems include the identification of amino acid residues in the antigen that interact with an antibody and elucidation of their individual contributions to binding affinity. We used fluorescence correlation spectroscopy (FCS) and alanine-scanning mutagenesis to character...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0607047
更新日期：2006-11-28 00:00:00
abstract：:A series of plastocyanin mutants have been constructed by site-directed mutagenesis and expressed in Escherichia coli to elucidate the interaction between plastocyanin and photosystem 1 in the photosynthetic electron-transfer chain. Leu-12 has been replaced with alanine, asparagine, glutamate, and lysine, while Tyr-83...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9520141
更新日期：1996-01-30 00:00:00
abstract：:Shaker potassium (K+) channels normally lack intrasubunit and intersubunit disulfide bonds. However, disulfide bonds are formed between Shaker subunits in intact cells exposed to oxidizing conditions. Upon electrophoresis under nonreducing conditions, intersubunit disulfide bond formation was detected by the presence ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961083s
更新日期：1996-09-17 00:00:00
abstract：:A manganese-dependent 3,4-dihydroxyphenylactate 2,3-dioxygenase from Arthrobacter globiformis strain CM-2 (MndD) cloned in Escherichia coli has been purified to homogeneity. Sedimentation equilibrium analysis indicates an alpha 4 homotetrameric holoenzyme structure (4 x 38,861 Da). Steady-state kinetic analysis of Mnd...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi951979h
更新日期：1996-01-09 00:00:00
abstract：:The laminin alpha3 chain is mainly expressed at the skin, and its C-terminal G domain has a critical role in multiple biological functions. We screened for biologically active sites on the mouse laminin alpha3 chain G domain using 107 synthetic peptides on coated plates and conjugated to Sepharose beads with HT1080 hu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901421t
更新日期：2009-11-10 00:00:00
abstract：:The ATPase activity of the (Ca2+-Mg2+)-ATPase reconstituted into bilayers of phosphatidylcholines depends on the fatty acyl chain length of the phospholipids. It is shown that the fluorescence response to Ca2+ of the ATPase modified with fluorescein isothiocyanate is also dependent on phospholipid structure and is int...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00371a043
更新日期：1986-11-18 00:00:00
abstract：:Minor groove specific DNA equilibrium binding peptides (lex) based on N-methylpyrrole-carboxamide and/or N-methylimidazolecarboxamide subunits have been modified with an O-methyl sulfonate ester functionality to target DNA methylation in the minor groove at Ade/Thy- and/or Gua/Cyt-rich sequences. HPLC and sequencing g...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0353272
更新日期：2003-12-09 00:00:00
abstract：:Venomous organisms have evolved a variety of structurally diverse peptide neurotoxins that target ion channels. Despite the lack of any obvious structural homology, unrelated toxins that interact with voltage-activated K(+) channels share a dyad motif composed of a lysine and a hydrophobic amino acid residue, usually ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0495681
更新日期：2004-07-13 00:00:00
abstract：:The three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor (RBI) from seeds of ragi (Eleusine coracana Gaertneri) has been determined in solution using multidimensional 1H and 15N NMR spectroscopy. The inhibitor consists of 122 amino acids, with 5 disulfide bridges, and belongs to the plant al...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00026a009
更新日期：1995-07-04 00:00:00
abstract：:The kinetic mechanism of NADPH-cytochrome P450 oxidoreductase (P450R) has been determined for the model reaction with cytochrome c3+. Although initial velocity studies show parallel patterns, consistent with a classical (one-site) ping-pong mechanism that precludes the formation of a ternary NADPH-P450R-cytochrome c3+...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00206a002
更新日期：1994-10-11 00:00:00
abstract：:A bifunctional molecule was genetically engineered which contained an amino-terminal effector domain that bound immunoglobulin Fc (fragment B of staphylococcal protein A) and a carboxyl-terminal domain that bound digoxin [a single-chain Fv (sFv)]. Effector and sFv binding properties were virtually identical with those...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00487a005
更新日期：1990-09-04 00:00:00
abstract：:The solution conformation of the type I collagen alpha-1 chain N-telopeptide has been studied by CD and 1H NMR spectroscopy at 600 MHz in CD3OH/H2O (60/40 v/v) and H2O solutions. The 19 amino acids form the N-terminal end of the alpha-1 polypeptide chain. By the combined application of several two-dimensional, phase-s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00446a006
更新日期：1989-10-03 00:00:00
abstract：:Sedimentation equilibrium results, obtained with bovine zinc-free insulin (with and without a component of proinsulin) at pH 7.0, I o.2, 25 degrees C, and up to a total concentration of 0.8 g/l., are shown to be consistent with three different polymerization patterns, all involving an isodesmic indefinite self-associa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00666a018
更新日期：1976-10-19 00:00:00
abstract：:End binding protein 1 (EB1) and cytoplasmic linker protein of 170 kDa (CLIP-170) are two well-studied microtubule plus-end-tracking proteins (+TIPs) that target growing microtubule plus ends in the form of comet tails and regulate microtubule dynamics. However, the mechanism by which they regulate microtubule dynamics...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300038t
更新日期：2012-04-10 00:00:00
abstract：:The solution conformation of an agalactosyl penta-antennary "bisected" N-linked glycan from hen ovomucoid has been determined using a combination of 1H-NMR NOE measurements and restrained molecular dynamics (MD) simulations. The majority of glycosidic linkages exhibited restricted torsional fluctuations about the glob...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00043a018
更新日期：1995-10-31 00:00:00
abstract：:Isolated alpha and beta chains from Xenopus laevis hemoglobin have been purified. The isolation procedure yields native alpha chains whose functional behavior has been characterized and compared with that of human alpha chains. Isolated beta chains in the presence of oxygen are characterized by low stability, and henc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00395a022
更新日期：1987-10-20 00:00:00
abstract：:During cellular uptake of fluorescently labeled cell-penetrating peptides (CPPs), intense fluorescent signals are commonly observed in the nucleus of the cell, suggesting intracellular CPP relocation and potential binding to the genome of the host. We therefore investigated the interaction of the CPP HIV-1 Tat(47-57) ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700416h
更新日期：2007-07-10 00:00:00
abstract：:The Rieske protein from Thermus thermophilus (TtRp) and a truncated version of the protein (truncTtRp), produced to achieve a low-pH crystallization condition, have been characterized using UV-visible and circular dichroism spectroscopies. TtRp and truncTtRp undergo a change in the UV-visible spectra with increasing p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901126u
更新日期：2009-10-20 00:00:00
abstract：:The proline catabolic enzyme Δ(1)-pyrroline-5-carboxylate dehydrogenase (ALDH4A1) catalyzes the NAD(+)-dependent oxidation of γ-glutamate semialdehyde to l-glutamate. In Saccharomyces cerevisiae, ALDH4A1 is encoded by the PUT2 gene and known as Put2p. Here we report the steady-state kinetic parameters of the purified ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500048b
更新日期：2014-03-04 00:00:00
abstract：:The S100 proteins comprise a group of EF-hand proteins that undergo a calcium-induced conformational change allowing them to interact with other proteins and produce a biological response. A unique feature of these proteins is the fact that they can form both homo- and heterodimers independent of calcium binding. The ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi802086a
更新日期：2009-03-10 00:00:00
abstract：:The major phospholipids, phosphatidylcholine and spingomyelin, of low density lipoprotein (LDL) are accessible to small amounts of Pr3+, suggesting that the head groups of all mobile phospholipids are on the surface of the particle in contact with the aqueous medium. The major source of the nuclear Overhauser effect e...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00607a002
更新日期：1978-07-11 00:00:00
