Abstract：
:Cesium-133 NMR studies have been carried out on suspended human erythrocytes and on perfused rat hearts in media containing CsCl. The resulting spectra exhibit two sharp resonances, arising from intra- and extracellular Cs+, separated in chemical shift by 1.0-1.4 ppm. Thus, intra- and extracellular resonances are easily resolved without the addition of paramagnetic shift reagents required to resolve resonances of the other alkali metal ions. Spin-lattice relaxation times in all cases are monoexponential and significantly shorter (3-4 times) for the intracellular component. When corrections are made for the pulse repetition rate, the total intensity of the intracellular and extracellular Cs+ resonances in erythrocytes is conserved, implying total observability of the intracellular pool. The uptake of Cs+ by erythrocytes occurs at approximately one-third the reported rate for K+ and was reduced by a factor of 2 upon addition of ouabain to the sample. These results indicate that 133Cs NMR is a promising tool for studying the distribution and transport of cesium ions in biological systems and, in some cases such as uptake by cellular Na,K-ATPase, for analysis of K+ ion metabolism.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Davis DG,Murphy E,London REdoi
10.1021/bi00410a003subject
Has Abstractpub_date
1988-05-17 00:00:00pages
3547-51issue
10eissn
0006-2960issn
1520-4995journal_volume
27pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:A computational method has been developed to predict inhibitor binding energy for untested inhibitor molecules. A neural network is trained from the electrostatic potential surfaces of known inhibitors and their binding energies. The algorithm is then able to predict, with high accuracy, the binding energy of unknown ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990830t
更新日期：1999-12-07 00:00:00
abstract：:In the present report we studied the interaction between the skeletal muscle ryanodine receptor and the ubiquitous S100A1 Ca2+ binding protein. S100A1 did not affect equilibrium [3H]ryanodine binding to purified rabbit skeletal muscle terminal cisternae at 100 microM free [Ca2+]. At nanomolar free [Ca2+], however, S10...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970160w
更新日期：1997-09-23 00:00:00
abstract：:In this paper, we examined the posttranslational modifications (PTMs) of high-mobility group A1 (HMGA1) proteins in PC-3 human prostate cancer cells that are either treated or not treated with a histone deacetylase inhibitor, sodium butyrate. We found that, from a reversed-phase C4 column, the HMGA1a protein eluted in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060504v
更新日期：2006-06-13 00:00:00
abstract：:Nickel is a fundamental micronutrient for cellular life, but it is toxic in soluble form at nonphysiological concentrations. Such potentially contradictory features required living organisms to develop efficient systems for nickel utilization and homeostasis. This is the case for incorporation of nickel into the activ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi6024676
更新日期：2007-03-20 00:00:00
abstract：:N-Linked sugar chains were liberated by hydrazinolysis from porcine zona pellucida glycoproteins obtained from ovarian follicular oocytes. Neutral sugar chains were separated from acidic ones by paper electrophoresis and fractionated with a serial lectin column chromatography and Bio-Gel P-4 column chromatography. The...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00222a012
更新日期：1991-02-26 00:00:00
abstract：:Although the binding of various forms of bleomycin to DNA has been studied extensively, the transient nature of the activated bleomycin species which ultimately attacks DNA has largely precluded direct examination of its physical interactions with DNA. In an attempt to map the minimum binding site required for this sp...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962957d
更新日期：1997-04-08 00:00:00
abstract：:Pokeweed antiviral protein (PAP) is a ribosome inactivating protein (RIP) that depurinates the sarcin/ricin loop (SRL) of rRNA, inhibiting protein synthesis. PAP depurinates viral RNA, and in doing so, lowers the infectivity of many plant viruses. The mechanism by which PAP accesses uncapped viral RNA is not known, im...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00598
更新日期：2017-11-14 00:00:00
abstract：:A novel truncated form (residues 1-214, with a randomized C-terminal tail) of the ligand-binding extracellular domain (ECD) of the human alpha1 glycine receptor (GlyR), with amino acids from the corresponding sequence of an acetylcholine binding protein (AChBP) substituted for two relatively hydrophobic membrane-proxi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi800659x
更新日期：2008-09-16 00:00:00
abstract：:Angiotensin converting enzyme (ACE) plays a critical role in the circulating or endocrine renin-angiotensin system (RAS) as well as the local regulation that exists in tissues such as the myocardium and skeletal muscle. Here we report the high-resolution crystal structures of testis ACE (tACE) in complex with the firs...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049480n
更新日期：2004-07-13 00:00:00
abstract：:Purine repressor (PurR) binding to specific DNA is enhanced by complexing with purines, whereas lactose repressor (LacI) binding is diminished by interaction with inducer sugars despite 30% identity in their protein sequences and highly homologous tertiary structures. Nonetheless, in switching from low- to high-affini...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0028643
更新日期：2001-07-10 00:00:00
abstract：:The C-terminal src kinase (CSK) is a ubiquitously expressed, cytosolic enzyme capable of phosphorylating and inactivating several plasma membrane-bound src-family protein tyrosine kinases in vitro [Nada, S., Okada, M., MacAuley, A., Cooper, J.A., & Nakagawa, H. (1990) Nature 351, 69-72; Bergman, M., Mustelin, T., Oetk...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9603940
更新日期：1996-09-10 00:00:00
abstract：:Lon is a homo-oligomeric ATP-dependent serine protease that functions in the degradation of damaged and certain regulatory proteins. This enzyme has emerged as a novel target in the development of antibiotics because of its importance in conferring bacterial virulence. In this study, we explored the mechanism by which...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7002789
更新日期：2007-06-05 00:00:00
abstract：:The role of the C-domain sites of cardiac troponin C in the modulation of the calcium signal remains unclear. In this study, we investigated the effects of hypertrophic cardiomyopathy-linked mutations A8V, E134D, and D145E in cardiac troponin C on the properties of the C-domain sites. The A8V mutation had essentially ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100400h
更新日期：2010-06-15 00:00:00
abstract：:The structure of a mutant Escherichia coli thioredoxin with a glutamic acid substituted for a conserved lysine at position 36 adjacent to the active site has been solved using molecular replacement and refined at 2.0-A resolution to a crystallographic residual of 19.9%. The mutant was crystallized in an orthorhombic s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00070a017
更新日期：1993-05-18 00:00:00
abstract：:The inheritance of DNA methylation patterns may play an important role in the stability of the differentiated state. We have therefore studied the inhibitory effects of DNA binding proteins on DNA methylation in vitro. Mouse L1210 cells grown in the presence of 5-azacytidine acquire hemimethylated sites in their DNA. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00326a021
更新日期：1985-02-26 00:00:00
abstract：:Group I intron RNAs contain a core of highly conserved helices flanked by peripheral domains that stabilize the core structure. In the Tetrahymena group I ribozyme, the P4, P5, and P6 helices of the core pack tightly against a three-helix subdomain called P5abc. Chemical footprinting and the crystal structure of the T...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982113p
更新日期：1999-03-09 00:00:00
abstract：:The luminescence of Cu-substituted cytochrome c exhibits temperature-dependent decay modes and spectral shifts at temperatures below 80 K. By comparison with the data from metal-substituted porphyrins, we determined intramolecular rate parameters. The observed tripdoublet decay time is 13 +/- 1 mus at 77 K and the qua...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00583a005
更新日期：1979-08-07 00:00:00
abstract：:The active-site zinc in human glutathione-dependent formaldehyde dehydrogenase (FDH) undergoes coenzyme-induced displacement and transient coordination to a highly conserved glutamate residue (Glu-67) during the catalytic cycle. The role of this transient coordination of the active-site zinc to Glu-67 in the FDH catal...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052554q
更新日期：2006-04-18 00:00:00
abstract：:Helicobacter pylori NikR (HpNikR) is a nickel-responsive transcription factor. In addition to a role in nickel homeostasis, HpNikR is proposed to serve as a master activator-repressor for H. pylori acid adaptation by directly or indirectly regulating the expression of a battery of genes. One potential mechanism of thi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi801742r
更新日期：2009-03-24 00:00:00
abstract：:Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA have been evolved to generate genetically encoded noncanonical amino acids (ncAAs). Use of tryptophan (Trp) analogues with pyrrole ring modification for their spatial and polarity tuning in enzyme activity and substrate specificity is still l...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00307
更新日期：2020-06-23 00:00:00
abstract：:Coenzyme A (CoASH) is the major low-molecular weight thiol in Staphylococcus aureus and a number of other bacteria; the crystal structure of the S. aureus coenzyme A-disulfide reductase (CoADR), which maintains the reduced intracellular state of CoASH, has recently been reported [Mallett, T.C., Wallen, J.R., Karplus, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi062299p
更新日期：2007-03-20 00:00:00
abstract：:Metal homeostasis and resistance in bacteria is maintained by a panel of metal-sensing transcriptional regulators that collectively control transition metal availability and mediate resistance to heavy metal xenobiotics, including As(III), Cd(II), Pb(II), and Hg(II). The ArsR family constitutes a superfamily of metal ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100490u
更新日期：2010-08-10 00:00:00
abstract：:Eukaryotic proteins with carboxyl-terminal CaaX motifs undergo three post-translational processing reactions-protein prenylation, endoproteolysis, and carboxymethylation. Two genes in yeast encoding CaaX endoproteases, AFC1 and RCE1, have been identified. Rce1p is solely responsible for proteolysis of yeast Ras protei...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9923611
更新日期：2000-04-11 00:00:00
abstract：:Dihydrouridine is absent from the tRNA of almost all hyperthermophiles and most Archaea but is ubiquitous in the tRNA of Eubacteria and Eukaryotes. In order to investigate whether this could be due to instability, the rate of ring opening of dihydrouridine was measured between 25 and 120 degrees C. The dihydrouridine ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi951577+
更新日期：1996-01-09 00:00:00
abstract：:(R)-N(delta)-(N'-Sulfodiaminophosphinyl)-L-ornithine (PSorn) is the active component of a phytotoxin, called phaseolotoxin, produced by Pseudomonas savastanoi pv. phaseolicola. PSorn acts as a potent transition state (TS) inhibitor of ornithine transcarbamoylase (OTCase, E.C. 2.1.3.3) that binds to the OTCase from Esc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi047432x
更新日期：2005-03-22 00:00:00
abstract：:The effect of DNA supercoiling on a sterically very demanding threading intercalation process is investigated here. We find that the threading rate of a dimeric ruthenium complex into a negatively supercoiled plasmid at low binding density is 2 orders of magnitude higher than into the cleaved linear form. Further satu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi802336p
更新日期：2009-02-24 00:00:00
abstract：:This paper reports the first identification of a fully functional hydrolyzing UDP-N-acetylglucosamine 2-epimerase from a bacterial source. The epimerase (known as SiaA or NeuC) from Neisseria meningitidis MC58 group B is shown to catalyze the conversion of UDP-GlcNAc into ManNAc and UDP in the first step of sialic aci...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048606d
更新日期：2004-11-09 00:00:00
abstract：:According to the X-ray crystallographic results from human deoxyhemoglobin, beta 99Asp at the alpha 1 Beta 2 interface forms hydrogen bonds with alpha 42Tyr and alpha 97Asn. To clarify the structural and functional roles of the hydrogen bond between alpha 97Asn and beta 99Asp, we have engineered a recombinant hemoglob...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952518z
更新日期：1996-05-28 00:00:00
abstract：:The early redox events involved in iron reduction and mobilization in mammalian ferritin have been investigated by several techniques. Sedimentation velocity measurements of ferritin samples with altered core sizes, prepared by partial reduction and Fe2+ chelation, suggest two different events occur during iron loss f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00430a033
更新日期：1989-02-21 00:00:00
abstract：:A detailed kinetic analysis of the catalytic trimer of aspartate transcarbamoylase containing the active site substitution H134A was performed to investigate the role of His 134 in the catalytic mechanism. Replacement of histidine by alanine resulted in decreases in the affinities for the two substrates, carbamoyl pho...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00143a031
更新日期：1992-07-21 00:00:00
