Kinetic mechanism of the Escherichia coli UDPMurNAc-tripeptide D-alanyl-D-alanine-adding enzyme: use of a glutathione S-transferase fusion.

abstract：:Rapid freeze-quench (RFQ) Mössbauer and stopped-flow absorption spectroscopy were used to monitor the ferritin ferroxidase reaction using recombinant (apo) frog M ferritin; the initial transient ferric species could be trapped by the RFQ method using low iron loading (36 Fe2+/ferritin molecule). Biphasic kinetics of f...

journal_title：Biochemistry

authors： Pereira AS,Small W,Krebs C,Tavares P,Edmondson DE,Theil EC,Huynh BH

更新日期：1998-07-14 00:00:00

abstract：:Michler's hydrol blue (MHB) is investigated with respect to photophysical properties in varied solvent environment and when bound to insulin and lysozyme fibrils. The MHB chromophore is shown to act like a molecular rotor and bind well to amyloid fibrils, where it exhibits a characteristic red-shift in its excitation ...

journal_title：Biochemistry

authors： Kitts CC,Beke-Somfai T,Nordén B

更新日期：2011-05-03 00:00:00

abstract：:Thermodynamic data for RNA 1 x 2 nucleotide internal loops are lacking. Thermodynamic data that are available for 1 x 2 loops, however, are for loops that rarely occur in nature. In order to identify the most frequently occurring 1 x 2 nucleotide internal loops, a database of 955 RNA secondary structures was compiled ...

journal_title：Biochemistry

authors： Badhwar J,Karri S,Cass CK,Wunderlich EL,Znosko BM

更新日期：2007-12-18 00:00:00

abstract：:Two series of experiments were carried out to characterize (a) peptide fragments of sarcoplasmic reticulum (SR) ATPase, based on proteolysis with different enzymes and distribution of known labels, and (b) specific labeling and functional inactivation patterns, following ATPase derivatization with dicyclohexylcarbodii...

journal_title：Biochemistry

authors： de Ancos JG,Inesi G

更新日期：1988-03-08 00:00:00

abstract：:The composition of the molybdate-stabilized glucocorticoid receptor (GR) complex has been investigated with a monoclonal antibody against the steroid-binding Mr 94 000 (94K) GR protein. It was concluded that one antibody molecule binds one 94K GR molecule. This finding constituted the basis for calculating the number ...

journal_title：Biochemistry

authors： Okret S,Wikström AC,Gustafsson JA

更新日期：1985-11-05 00:00:00

abstract：:Inefficient maturation and trafficking to the cell surface of the cystic fibrosis transmembrane conductance regulator (CFTR) is the primary cause of cystic fibrosis. CFTR protein that fails to mature accumulates as an immature core-glycosylated protein and is rapidly degraded. To determine how the structures of mature...

journal_title：Biochemistry

authors： Chen EY,Bartlett MC,Clarke DM

更新日期：2000-04-04 00:00:00

abstract：:Proteins from thermophiles possess high thermostability. The stabilization mechanisms differ between archaeal and bacterial proteins, whereby archaeal proteins are mainly stabilized via hydrophobic interactions and bacterial proteins by ion pairs. High stability is an important factor in promoting protein evolution, b...

journal_title：Biochemistry

authors： Takano K,Aoi A,Koga Y,Kanaya S

更新日期：2013-07-16 00:00:00

abstract：:Adipocyte lipid-binding protein (ALBP) is the adipocyte member of an intracellular hydrophobic ligand-binding protein family. ALBP is phosphorylated by the insulin receptor kinase upon insulin stimulation. The crystal structure of recombinant murine ALBP has been determined and refined to 2.5 A. The final R factor for...

journal_title：Biochemistry

authors： Xu Z,Bernlohr DA,Banaszak LJ

更新日期：1992-04-07 00:00:00

abstract：:31P NMR spectra were obtained from poly(dA-dT) . poly(dA-dT) fibers which gave an X-ray diffraction pattern similar to that of the A form of natural deoxyribonucleic acid (DNA). The analysis of the line shape indicated that the A form of poly(dA-dT) . poly(dA-dT) has a single uniform backbone conformation; the orienta...

journal_title：Biochemistry

authors： Shindo H,Wooten JB,Zimmerman SB

更新日期：1981-02-17 00:00:00

abstract：:The specificity of valyl-, phenylalanyl-, and tyrosyl-tRNA synthetases from yeast has been examined by a series of stringent tests designed to eliminate the possibility of artefactual interference. Valyl-tRNA synthetase, as well as activating a number of amino acid analogues, will accept alanine, cysteine, isoleucine,...

journal_title：Biochemistry

authors： Igloi GL,von der Haar F,Cramer F

更新日期：1978-08-22 00:00:00

abstract：:We have used limited proteolysis of affinity-labeled estrogen receptors (ER), coupled with antireceptor antibody immunoreactivity, to assess structural features of ER and the relatedness of ER from MCF-7 human breast cancer and rat uterine cells. MCF-7 ER preparations covalently labeled with [3H]tamoxifen aziridine [(...

journal_title：Biochemistry

authors： Katzenellenbogen BS,Elliston JF,Monsma FJ Jr,Springer PA,Ziegler YS,Greene GL

更新日期：1987-04-21 00:00:00

abstract：:The solution conformation of the DNA duplex d(C1G2C3A4C5L6C7A8C9G10C11).d(G12C13G14T15G16T17G18T19G20C21G22 ) containing the 2'-deoxyribonolactone lesion (L6) in the middle of the sequence has been investigated by NMR spectroscopy and restrained molecular dynamics calculations. Interproton distances have been obtained...

journal_title：Biochemistry

authors： Jourdan M,Garcia J,Defrancq E,Kotera M,Lhomme J

更新日期：1999-03-30 00:00:00

abstract：:4-Azido-2-nitrophenyl phosphate (ANPP) was synthesized and characterized. ANPP, unlabeled or labeled by 32P, was used as a photoreactive analogue of Pi to study the Pi binding site(s) in isolated F1-ATPase and inside-out particles from beef heart mitochondria. In the dark, the phosphate bond of ANPP was cleaved by alk...

journal_title：Biochemistry

authors： Lauquin G,Pougeois R,Vignais PV

更新日期：1980-09-30 00:00:00

abstract：:The UDP-N-acetylglucosamine (UDP-GlcNAc) acyltransferase, encoded by lpxA, catalyzes the first step of lipid A biosynthesis in Gram-negative bacteria, the (R)-3-hydroxyacyl-ACP-dependent acylation of the 3-OH group of UDP-GlcNAc. Recently, we demonstrated that the Arabidopsis thaliana orthologs of six enzymes of the b...

journal_title：Biochemistry

authors： Joo SH,Chung HS,Raetz CR,Garrett TA

更新日期：2012-05-29 00:00:00

abstract：:High-resolution phosphorus-31 nuclear magnetic resonance (31P NMR) spectra of wild-type and mutant strains of Saccharomyces cerevisiae were observed at a frequency of 145.7 MHz. Levels of various phosphorus metabolites were investigated upon addition of glucose under both aerobic and anaerobic conditions. Three mutant...

journal_title：Biochemistry

authors： Navon G,Shulman RG,Yamane T,Eccleshall TR,Lam KB,Baronofsky JJ,Marmur J

更新日期：1979-10-16 00:00:00

abstract：:Peptides corresponding to portions of curaremimetic neurotoxin loop 2 and to a structurally similar segment of rabies virus glycoprotein were synthetically modified in order to gain information on structure-function relationships of neurotoxin loop 2 interactions with the acetylcholine receptor. Binding of synthetic p...

journal_title：Biochemistry

authors： Lentz TL

更新日期：1991-11-12 00:00:00

abstract：:Adriamycin plays a prominent role in the treatment of leukemia and solid tumors in man. The mode of interaction of adriamycin with its nuclear target, responsible for its therapeutic effect, is known [Berman, H. M., & Young, P.R. (1981) Annu. Rev. Biophys. Bioeng. 10, 87-114]. The planar anthracycline moiety of adriam...

journal_title：Biochemistry

authors： Goormaghtigh E,Brasseur R,Huart P,Ruysschaert JM

更新日期：1987-03-24 00:00:00

abstract：:The reaction of enantiomerically pure (2S)-N-acetyl-L-alanyl-L-phenylalanyl alpha-chloroethane with gamma-chymotrypsin was studied as a probe of the mechanism of inactivation of serine proteases by peptidyl chloroalkanes. It was determined crystallographically that the peptidyl chloroethane alkylates His57 with retent...

journal_title：Biochemistry

authors： Kreutter K,Steinmetz AC,Liang TC,Prorok M,Abeles RH,Ringe D

更新日期：1994-11-22 00:00:00

abstract：:Thermodynamics of 39 oligonucleotides with internal G.T mismatches dissolved in 1 M NaCl were determined from UV absorbance versus temperature profiles. These data were combined with literature values of six sequences to derive parameters for 10 linearly independent trimer and tetramer sequences with G.T mismatches an...

journal_title：Biochemistry

authors： Allawi HT,SantaLucia J Jr

更新日期：1997-08-26 00:00:00

abstract：:The binding of propidium to poly(dA).poly(dT) [poly(dA.dT)] and to poly[d(A-T)].poly[d(A-T)] [poly[d(A-T)2]] has been compared under a variety of solution conditions by viscometric titrations, binding studies, and kinetic experiments. The binding of propidium to poly[d(A-T)2] is quite similar to its binding to calf th...

journal_title：Biochemistry

authors： Wilson WD,Wang YH,Krishnamoorthy CR,Smith JC

更新日期：1985-07-16 00:00:00

abstract：:The introduction into peptide chains of alpha-aminoisobutyric acid (Aib) has proven to stabilize the helical structure in short peptides by restricting the available range of polypeptide backbone conformations. In order to evaluate the potential stabilizing effect of Aib at the protein level, we have studied the confo...

journal_title：Biochemistry

authors： De Filippis V,De Antoni F,Frigo M,Polverino de Laureto P,Fontana A

更新日期：1998-02-10 00:00:00

abstract：:We describe the crystal structure of human epidermal-type fatty acid binding protein (E-FABP) that was recently found to be highly upregulated in human psoriatic keratinocytes. To characterize E-FABP with respect to ligand-binding properties and tertiary structure, we cloned the respective cDNA, overexpressed the prot...

journal_title：Biochemistry

authors： Hohoff C,Börchers T,Rüstow B,Spener F,van Tilbeurgh H

更新日期：1999-09-21 00:00:00

abstract：:The binding of rabbit globin mRNA to the 25-kDa cap binding protein eIF-4E from human erythrocytes was found to be 5.3-fold stronger than the binding of the cap analogue m7GpppG to eIF-4E [Gross et al. (1990) Biochemistry 29, 5008-5012]. In order to investigate whether this effect is due to the longer sequence of nucl...

journal_title：Biochemistry

authors： Carberry SE,Friedland DE,Rhoads RE,Goss DJ

更新日期：1992-02-11 00:00:00

abstract：:The molecular mass (M(r)) of the nickel- and iron-sulfur-containing enzyme CO dehydrogenase from Clostridium thermoaceticum was determined by sedimentation equilibrium ultracentrifugation to be 300,000 +/- 30,000 Da. Since the enzyme is known to contain equal numbers of two types of subunits (M(r) = 82,000 Da for alph...

journal_title：Biochemistry

authors： Xia J,Sinclair JF,Baldwin TO,Lindahl PA

更新日期：1996-02-13 00:00:00

abstract：:The only identified component of the scrapie prion is PrPSc, a glycosylinositol phospholipid (GPI)-linked protein that is derived from the cellular isoform (PrPC) by an as yet unknown posttranslational event. Analysis of the PrPSc GPI has revealed six different glycoforms, three of which are unprecedented. Two of the ...

journal_title：Biochemistry

authors： Stahl N,Baldwin MA,Hecker R,Pan KM,Burlingame AL,Prusiner SB

更新日期：1992-06-02 00:00:00

abstract：:The relationship between cholesterol and ubiquinone synthesis in rat intestinal epithelial cell cultures was examined by using 3 beta-[2-(diethylamino)ethoxy]androst-5-en-17-one hydrochloride (U18666A). Addition of U18666A to cells caused a greater than 90% inhibition of incorporation of [3H]acetate into cholesterol a...

journal_title：Biochemistry

authors： Sexton RC,Panini SR,Azran F,Rudney H

更新日期：1983-12-06 00:00:00

abstract：:Interaction of affinity chromatographically purified antihistone H3 and antihistone H4 with isolated HeLa core particles, followed by separation of unreacted and reacted particles by sedimentation, demonstrates that every core particle contains these histones. Taken together with our previous data indicating the prese...

journal_title：Biochemistry

authors： Bustin M,Simpson RT,Sperling R,Goldblatt D

更新日期：1977-11-29 00:00:00

abstract：:Numerous studies have been published stressing the importance of finding ligands that can bind specifically to DNA secondary structures. Several have identified ligands that are presented as having specific binding to the G-quadruplex; however, these were not originally tested on the complementary i-motif structure. T...

journal_title：Biochemistry

authors： Abdelhamid MAS,Gates AJ,Waller ZAE

更新日期：2019-01-29 00:00:00

abstract：:Heme oxygenase (HO) catalyzes the regiospecific cleavage of the porphyrin ring of heme using reducing equivalents and O2 to produce biliverdin, iron, and CO. Because CO has a cytoprotective effect through the p38-MAPK pathway, HO is a potential therapeutic target in cancer. In fact, inhibition of the HO isoform HO-1 r...

journal_title：Biochemistry

authors： Sugishima M,Higashimoto Y,Oishi T,Takahashi H,Sakamoto H,Noguchi M,Fukuyama K

更新日期：2007-02-20 00:00:00

abstract：:The marked difference between the intra- and extracellular 31P NMR chemical shifts of various phosphoryl compounds when added to a red cell suspension may be largely understood in terms of the effects of hemoglobin on the 31P NMR chemical shifts. The presence of [oxy- or (carbonmonoxy)-] hemoglobin inside the red cell...

journal_title：Biochemistry

authors： Kirk K,Kuchel PW

更新日期：1988-11-29 00:00:00