Abstract:
:Inefficient maturation and trafficking to the cell surface of the cystic fibrosis transmembrane conductance regulator (CFTR) is the primary cause of cystic fibrosis. CFTR protein that fails to mature accumulates as an immature core-glycosylated protein and is rapidly degraded. To determine how the structures of mature and immature CFTR are different, we compared the properties of CFTR that had been expressed in the presence or absence of the proteasome inhibitor, MG-132 (carbobenzoxy-L-leucyl-L-leucyl-L-leucinal). Transient expression of wild-type CFTR in the presence of submicromolar concentrations of MG-132 blocks maturation of the protein. We found that expression of CFTR in the presence of MG-132 trapped the protein in a trypsin-sensitive conformation. In addition, the structure of the carboxyl-terminus of immature and mature CFTR differed as histidine-tagged mature CFTR was preferentially recovered by metal-chelate chromatography. No chloride channel activity was detected when membranes containing immature CFTR were fused with planar lipid bilayers. These results show that expression of CFTR in the presence of MG-132 traps the protein in an altered conformation that may be inactive.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Chen EY,Bartlett MC,Clarke DMdoi
10.1021/bi992620msubject
Has Abstractpub_date
2000-04-04 00:00:00pages
3797-803issue
13eissn
0006-2960issn
1520-4995pii
bi992620mjournal_volume
39pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::"Charged-to alanine" scanning mutagenesis of the catalytic subunit of the Saccharomyces cerevisiae cAMP-dependent protein kinase (C1) identified three glutamate residues, E171, E214, and E274, that are involved in the recognition of a peptide substrate, kemptide (Leu1Arg2Arg3Ala4Ser5Leu6Gly7). These glutamate residues...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00236a001
更新日期:1991-06-04 00:00:00
abstract::The laminin alpha3 chain is mainly expressed at the skin, and its C-terminal G domain has a critical role in multiple biological functions. We screened for biologically active sites on the mouse laminin alpha3 chain G domain using 107 synthetic peptides on coated plates and conjugated to Sepharose beads with HT1080 hu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901421t
更新日期:2009-11-10 00:00:00
abstract::Binding of deoxyribonucleoside 5'-triphosphates to DNA polymerase I of Escherichia coli was measured by using a microscale nonequilibrium dialysis method. It allowed rapid and economic measurement of dissociation constants, with negligible interfering side reactions. A stoichiometry of 1 mol of nucleoside 5'-triphosph...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00335a033
更新日期:1985-07-02 00:00:00
abstract::Human transcriptional coactivator P/CAF (p300/CBP-associating factor) is a histone acetyltransferase (HAT) and is a member of the GNAT (GCN5 related N-acetyltransferases) superfamily. P/CAF was originally identified by its ability to activate transcription of a variety of genes through its interaction with p300/CBP. T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001272h
更新日期:2000-10-03 00:00:00
abstract::In the preceding accompanying paper [Shu, X., et al. (2007) Biochemistry 46, 12005-12013], the 1.5 A resolution crystal structure of green fluorescent protein (GFP) variant S65T/H148D is presented, and the possible consequences of an unusual short hydrogen bond ( journal_title:Biochemistry pub_type: 杂志文章 doi:10.1021/bi700904a 更新日期:2007-10-30 00:00:00
abstract::Heme oxygenase (HO), from the pathogenic bacterium N. meningitidis(NmHO), which secures host iron, shares many properties with mammalian HOs but also exhibits some key differences. The crystal structure appears more compact, and the crystal-undetected C-terminus interacts with substrate in solution. The unique nature ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200978g
更新日期:2011-10-18 00:00:00
abstract::Cyclobutane pyrimidine dimers (Pyr < > Pyr) are the major DNA photoproducts induced by the UV component of solar radiation. Photoreactivating enzyme (DNA photolyase) repairs DNA by utilizing the energy of visible light to break the cyclobutane ring of the dimer. Photolyases are monomeric proteins of 50-60 kDa with sto...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi00167a001
更新日期:1994-01-11 00:00:00
abstract::The following polynucleotides containing the antibiotic tubercidin (Tu; 4-amino-7-beta-D-ribofuranosylpyrrolo-[2,3-d]pyrimidine) were enzymatically synthesized by polymerization of adenosine 5'-diphosphate-tubercidin 5'-diphosphate mixtures with polynucleotide phosphorylase: poly(A2,Tu), poly(A,Tu2), and poly(Tu). The...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00512a030
更新日期:1981-04-28 00:00:00
abstract::The flavoprotein component (SiR-FP) of the sulfite reductase from Escherichia coli is an octamer containing one FAD and one FMN per polypeptide chain. SiR-FP60, a SiR-FP fragment starting with alanine-52, was overexpressed in E. coli and purified as a monomer. The N-terminal part of the native protein contains thus al...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9728699
更新日期:1998-04-28 00:00:00
abstract::Lactone-hydrolyzing enzymes derived from some Bacillus species are capable of disrupting quorum sensing in bacteria that use N-acyl-l-homoserine lactones (AHLs) as intercellular signaling molecules. Despite the promise of these quorum-quenching enzymes as therapeutic and anti-biofouling agents, the ring opening mechan...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061238o
更新日期:2006-11-07 00:00:00
abstract::Differential expression of subunit genes from the nicotinic acetylcholine receptor (AChR) superfamily yields distinct receptor subtypes. As each AChR subtype has a specific subunit composition and many subunit combinations appear not to be expressed, each subunit must contain some information leading to proper assembl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00254a031
更新日期:1994-12-20 00:00:00
abstract::Furamidine and related diamidines represent a promising series of drugs active against widespread parasites, in particular the Pneumocystic carinii pathogen. In this series, the phenylfuranbenzimidazole diamidine derivative DB293 was recently identified as the first unfused aromatic dication capable of forming stacked...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0108453
更新日期:2001-08-21 00:00:00
abstract::The side-chain dynamics of methyl groups in two structurally related proteins from the fibronectin type III (fnIII) superfamily, the third fnIII domain from human tenascin (TNfn3) and the tenth fnIII domain from human fibronectin (FNfn10), have been studied by NMR spectroscopy. Side-chain order parameters reveal that ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035658e
更新日期:2004-02-10 00:00:00
abstract::Rabbit aortic smooth muscle cells were evaluated for their ability to synthesize and accumulate glycosaminoglycans (GAGs). Because of the sensitivity of the microtechniques utilized, it is possible to determine the specific radioactivity of the GAGs obtained after radioactive incorporation of [35S]SO4(2-) and [14C]glu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00550a026
更新日期:1980-04-29 00:00:00
abstract::We have investigated, both experimentally and theoretically, the efflux of carboxyfluorescein (a self-quenching fluorescent dye) from vesicles of different sizes and lipid species (POPC, DOPC) after having added the bee venom peptide melittin. This comprises quantitative analyses regarding the extent of lipid-associat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi971009p
更新日期:1998-02-24 00:00:00
abstract::The role of acidic amino acid residues in cation recognition and selectivity by the Na+/dicarboxylate cotransporter, NaDC-1, was investigated by site-directed mutagenesis and expression in Xenopus oocytes. Four of the residues tested, Asp-52, Glu-74, Glu-101, and Glu-332, were found to be unimportant for transport act...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990076b
更新日期:1999-06-08 00:00:00
abstract::Treatment of human lymphoid cells with methyl methanesulfonate (MMS) results in discontinuities in cellular DNA. Discontinuities can be detected by sedimentation through neutral sucrose gradients after digestion with S1 nuclease on top of the gradient and by benzoylated naphthoylated diethylaminoethylcellulose (BND-ce...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00639a024
更新日期:1977-10-04 00:00:00
abstract::Peptide-membrane interactions are important for understanding the binding, partitioning, and folding of membrane proteins; the activity of antimicrobial and fusion peptides; and a number of other processes. We describe molecular dynamics simulations (10-25 ns) of two pentapeptides Ace-WLXLL (with X = Arg or Lys side c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi027001j
更新日期:2003-08-05 00:00:00
abstract::During cellular uptake of fluorescently labeled cell-penetrating peptides (CPPs), intense fluorescent signals are commonly observed in the nucleus of the cell, suggesting intracellular CPP relocation and potential binding to the genome of the host. We therefore investigated the interaction of the CPP HIV-1 Tat(47-57) ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi700416h
更新日期:2007-07-10 00:00:00
abstract::Two distinct regions of angiogenin are critical for angiogenic activity: a catalytic site capable of cleaving RNA and a noncatalytic site, encompassing residues 60-68, which may bind to an endothelial cell-surface receptor [Hallahan, T. W., Shapiro, R., & Vallee, B. L. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 2222-222...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00149a036
更新日期:1992-09-01 00:00:00
abstract::Chicken histone H4, labeled separately at Met-84 with N-[[(iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid and 5-(iodoacetamido)fluorescein, was reassociated with unlabeled histones H2A, H2B, and H3 and 146 base pairs of DNA to produce fluorescently labeled nucleosomes having physical characteristics virtually...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00366a010
更新日期:1986-09-09 00:00:00
abstract::Human cysteine-rich secretory protein 3 (CRISP-3; also known as SGP28) belongs to a family of closely related proteins found in mammals and reptiles. Some mammalian CRISPs are known to be involved in the process of reproduction, whereas some of the CRISPs from reptiles are neurotoxin-like substances found in lizard sa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048823e
更新日期:2004-10-12 00:00:00
abstract::We have investigated some properties related to interaction with DNA and recognition of AT-rich sequences of netropsin-oxazolopyridocarbazole (Net-OPC) (Mrani et al., 1990), which is a hybrid groove-binder-intercalator. The hybrid molecule Net-OPC binds to poly[d(A-T)] at two different sites with Kapp values close to ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00220a029
更新日期:1991-02-12 00:00:00
abstract::We have reexamined the binding properties of the antitumor drug daunomycin using double-helical oligonucleotides 16 base pairs long that were designed to contain preferred binding sites for the drug. The preferred sites are contained in a six base pair core which is flanked on the 5' and 3' ends by tracts of adenines....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00170a011
更新日期:1994-02-01 00:00:00
abstract::We have investigated perturbations of the triplet-state properties of Trp residues in bacteriophage T4 lysozyme caused by point mutations using low-temperature phosphorescence and optical detection of triplet-state magnetic resonance (ODMR) spectroscopy. Five temperature-sensitive mutants have been studied in detail. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00431a041
更新日期:1989-03-07 00:00:00
abstract::Proteases specific for cleavage after acidic residues have been implicated in several disease states, including epidermolysis, inflammation, and viral processing. A serine protease with specificity toward glutamic acid substrates (Glu-SGP) has been crystallized in the presence of a tetrapeptide ligand and its structur...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00094a001
更新日期:1993-11-02 00:00:00
abstract::The ferritin IRE, a highly conserved (96-99% in vertebrates) mRNA translation regulatory element in animal mRNA, was studied by molecular modeling (using MC-SYM and DOCKING) and by NMR spectroscopy. Cobalt(III) hexammine was used to model hydrated Mg2+. IRE isoforms in other mRNAs regulate mRNA translation or stabilit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9719814
更新日期:1998-02-10 00:00:00
abstract::The interactions of Alzheimer's amyloid beta-peptide with cyclodextrins were studied by (1)H NMR: the translational diffusion coefficient of the peptide and chemical shift changes were studied by the presence of variable concentrations of cyclodextrins. For the full-length peptide, Abeta(1-40), the combined results of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036254p
更新日期:2004-05-25 00:00:00
abstract::Marijuana is the most widely abused illegal drug, and its spectrum of effects suggests that several receptors are responsible for the activity. Two cannabinoid receptor subtypes, CB1 and CB2, have been identified, but the complex pharmacological properties of exogenous cannabinoids and endocannabinoids are not fully e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200010k
更新日期:2011-06-28 00:00:00
abstract::The coat protein of bacteriophage Pf3 forms discrete and stable ion channels of uniform size in planar bilayers of asolectin. Its primary sequence suggests a channel formed by a bundle of transmembrane helices. Since the apparent transmembrane region only consists of strongly hydrophobic residues, it represents a new ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00167a037
更新日期:1994-01-11 00:00:00