当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > A glutamic acid specific serine protease utilizes a novel histidine triad in substrate binding.

A glutamic acid specific serine protease utilizes a novel histidine triad in substrate binding.

Abstract：

:Proteases specific for cleavage after acidic residues have been implicated in several disease states, including epidermolysis, inflammation, and viral processing. A serine protease with specificity toward glutamic acid substrates (Glu-SGP) has been crystallized in the presence of a tetrapeptide ligand and its structure determined and refined to an R-factor of 17% at 2.0-A resolution. This structure provides an initial description of the design of proteolytic specificity for negatively charged residues. While the overall fold of Glu-SGP closely resembles that observed in the pancreatic-type serine proteases, stabilization of the negatively charged substrate when bound to this protein appears to involve a more extensive part of the protease than previously observed. The substrate carboxylate is bound to a histidine side chain, His213, which provides the primary electrostatic compensation of the negative charge on the substrate, and to two serine hydroxyls, Ser192 and Ser216. Glu-SGP displays maximum activity at pH 8.3, and assuming normal pKa's, the glutamate side chain and His213 will be negatively charged and neutral, respectively, at this pH. In order for His213 to carry a positive charge at the optimal pH, its pKa will have to be raised by at least two units. An alternative mechanism for substrate charge compensation is suggested that involves a novel histidine triad, His213, His199, and His228, not observed in any other serine protease. The C-terminal alpha-helix, ubiquitous to all pancreatic-type proteases, is directly linked to this histidine triad and may also play a role in substrate stabilization.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Nienaber VL,Breddam K,Birktoft JJ

doi

10.1021/bi00094a001

subject

Has Abstract

pub_date

1993-11-02 00:00:00

pages

11469-75

issue

43

eissn

0006-2960

issn

1520-4995

journal_volume

32

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Structural and functional analysis of EcoRI DNA methyltransferase by proteolysis.

    abstract：:Native EcoRI DNA methyltransferase (Mtase, Mr 38,050) is proteolyzed by trypsin to generate an intermediate 36-kDa fragment (p36) followed by the formation of two polypeptides of Mr 23,000 and 13,000 (p23 and p13, respectively). Protein sequence analysis of the tryptic fragments indicates that p36 results from removal...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00225a030

    authors： Reich NO,Maegley KA,Shoemaker DD,Everett E

    更新日期：1991-03-19 00:00:00

  • Subtilisin BPN' variants: increased hydrolytic activity on surface-bound substrates via decreased surface activity.

    abstract：:Site-directed mutagenesis and random mutagenesis were used to produce variants of subtilisin BPN' (Bacillus amyloliquefaciens) protease with variable surface adsorption properties. Protease adsorption and peptide hydrolysis rate were measured for these variants using a model substrate consisting of a peptide covalentl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi951990h

    authors： Brode PF 3rd,Erwin CR,Rauch DS,Barnett BL,Armpriester JM,Wang ES,Rubingh DN

    更新日期：1996-03-12 00:00:00

  • Circular dichroism and UV resonance raman study of the impact of alcohols on the Gibbs free energy landscape of an alpha-helical peptide.

    abstract：:We used CD and UV resonance Raman spectroscopy to study the impact of alcohols on the conformational equilibria and relative Gibbs free energy landscapes along the Ramachandran Psi-coordinate of a mainly poly-Ala peptide, AP with an AAAAA(AAARA)(3)A sequence. 2,2,2-Trifluoroethanol (TFE) most stabilizes the alpha-heli...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100176a

    authors： Xiong K,Asher SA

    更新日期：2010-04-20 00:00:00

  • The crystal structure of the C-terminal truncated apolipoprotein A-I sheds new light on amyloid formation by the N-terminal fragment.

    abstract：:Apolipoprotein A-I (apoA-I) is the main protein of plasma high-density lipoproteins (HDL, or good cholesterol) that remove excess cell cholesterol and protect against atherosclerosis. In hereditary amyloidosis, mutations in apoA-I promote its proteolysis and the deposition of the 9-11 kDa N-terminal fragments as fibri...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/bi2017014

    authors： Gursky O,Mei X,Atkinson D

    更新日期：2012-01-10 00:00:00

  • Structure of an active water molecule in the water-oxidizing complex of photosystem II as studied by FTIR spectroscopy.

    abstract：:The vibrations of a water molecule in the water-oxidizing complex (WOC) of photosystem II were detected for the first time using Fourier transform infrared (FTIR) spectroscopy. In a flash-induced FTIR difference spectrum upon the S(1)-to-S(2) transition, a pair of positive and negative bands was observed at 3618 and 3...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001040i

    authors： Noguchi T,Sugiura M

    更新日期：2000-09-12 00:00:00

  • Truncated carboxyl-terminal fragments of beta-amyloid precursor protein are processed to amyloid beta-proteins 40 and 42.

    abstract：:We previously showed that beta-amyloid precursor protein (APP) is cleaved not only in the middle of the membrane (gamma-cleavage) but also at novel cleavage sites close to the membrane/cytoplasmic boundary (epsilon-cleavage), releasing APP intracellular domains (AICDs) 49-99 and 50-99. To learn more about the relation...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049399k

    authors： Funamoto S,Morishima-Kawashima M,Tanimura Y,Hirotani N,Saido TC,Ihara Y

    更新日期：2004-10-26 00:00:00

  • Orientation of type VI collagen monomers in molecular aggregates.

    abstract：:Type VI collagen, prepared from guanidine extracts of human amnion, contains very little monomeric material, the major forms being dimers and tetramers. In order to study the orientation of the molecules in these aggregates, they were digested with pepsin followed by bacterial collagenase. Two fragments were isolated,...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00435a020

    authors： Kuo HJ,Keene DR,Glanville RW

    更新日期：1989-05-02 00:00:00

  • Noncompetitive Allosteric Antagonism of Death Receptor 5 by a Synthetic Affibody Ligand.

    abstract：:Fatty acid-induced upregulation of death receptor 5 (DR5) and its cognate ligand, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), promotes hepatocyte lipoapoptosis, which is a key mechanism in the progression of fatty liver disease. Accordingly, inhibition of DR5 signaling represents an attractive str...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00529

    authors： Vunnam N,Szymonski S,Hirsova P,Gores GJ,Sachs JN,Hackel BJ

    更新日期：2020-10-13 00:00:00

  • Elastin binds to a multifunctional 67-kilodalton peripheral membrane protein.

    abstract：:Elastin binding proteins from plasma membranes of elastin-producing cells were isolated by affinity chromatography on immobilized elastin peptides. Three proteins of 67, 61, and 55 kDa were released from the elastin resin by guanidine/detergent, soluble elastin peptides, synthetic peptide VGVAPG, or galactoside sugars...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00435a014

    authors： Mecham RP,Hinek A,Entwistle R,Wrenn DS,Griffin GL,Senior RM

    更新日期：1989-05-02 00:00:00

  • Transcriptional activity of a fluorinated vitamin D analog on VDR-RXR-mediated gene expression.

    abstract：:The transcriptional activity of the hexafluorinated derivative of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], 26,26,26,27,27,27-hexafluoro-1,25-dihydroxyvitamin D3 [F6-1,25-(OH)2D3], was examined in cultured cells by a transient expression assay (CAT assay) using expression vectors for the rat nuclear vitamin D3 receptor...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00001a045

    authors： Sasaki H,Harada H,Handa Y,Morino H,Suzawa M,Shimpo E,Katsumata T,Masuhiro Y,Matsuda K,Ebihara K

    更新日期：1995-01-10 00:00:00

  • Nucleotide Excision Repair and Impact of Site-Specific 5',8-Cyclopurine and Bulky DNA Lesions on the Physical Properties of Nucleosomes.

    abstract：:The nonbulky 5',8-cyclopurine DNA lesions (cP) and the bulky, benzo[ a]pyrene diol epoxide-derived stereoisomeric cis- and trans- N2-guanine adducts (BPDE-dG) are good substrates of the human nucleotide excision repair (NER) mechanism. These DNA lesions were embedded at the In or Out rotational settings near the dyad ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b01066

    authors： Shafirovich V,Kolbanovskiy M,Kropachev K,Liu Z,Cai Y,Terzidis MA,Masi A,Chatgilialoglu C,Amin S,Dadali A,Broyde S,Geacintov NE

    更新日期：2019-02-12 00:00:00

  • Cytotoxic effects of inhibitors of de novo pyrimidine biosynthesis upon Plasmodium falciparum.

    abstract：:The malarial parasite Plasmodium falciparum can only synthesize pyrimidine nucleotides via the de novo pathway which is therefore a suitable target for development of antimalarial drugs. New assay procedures have been developed using high-pressure liquid chromatography (HPLC) which enable concurrent measurement of pyr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00183a033

    authors： Seymour KK,Lyons SD,Phillips L,Rieckmann KH,Christopherson RI

    更新日期：1994-05-03 00:00:00

  • Lipoprotein lipase catalyzed hydrolysis of water-soluble p-nitrophenyl esters. Inhibition by apolipoprotein C-II.

    abstract：:Bovine milk lipoprotein lipase (LpL) catalyzes the hydrolysis of the water-soluble esters p-nitrophenyl acetate (PNPA) and p-nitrophenyl butyrate (PNPB). The same protein and same active site are involved in hydrolysis of water-soluble p-nitrophenyl esters and emulsified trioleoylglycerol since (a) trioleoylglycerol h...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00269a038

    authors： Quinn DM,Shirai K,Jackson RL,Harmony JA

    更新日期：1982-12-21 00:00:00

  • Oxidative protein folding in vitro: a study of the cooperation between quiescin-sulfhydryl oxidase and protein disulfide isomerase.

    abstract：:The flavin-dependent quiescin-sulfhydryl oxidase (QSOX) inserts disulfide bridges into unfolded reduced proteins with the reduction of molecular oxygen to form hydrogen peroxide. This work investigates how QSOX and protein disulfide isomerase (PDI) cooperate in vitro to generate native pairings in two unfolded reduced...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801604x

    authors： Rancy PC,Thorpe C

    更新日期：2008-11-18 00:00:00

  • Sensitivity of flavin fluorescence dynamics in neuronal nitric oxide synthase to cofactor-induced conformational changes and dimerization.

    abstract：:The fluorescence intensity of the two flavin prosthetic groups, FMN and FAD, in neuronal nitric oxide synthase (nNOS) was found to decay highly nonexponentially, being best described by four fluorescence lifetimes. This excited state heterogeneity is the result of multiple flavin quenching sites which are due to sever...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981138l

    authors： Brunner K,Tortschanoff A,Hemmens B,Andrew PJ,Mayer B,Kungl AJ

    更新日期：1998-12-15 00:00:00

  • An unusual catalytic subunit for the cAMP-dependent protein kinase of Dictyostelium discoideum.

    abstract：:The cAMP-dependent protein kinase (cAPK) plays an essential role during differentiation and fruit morphogenesis in Dictyostelium discoideum. The presence of an open reading frame on the gene, pkaC (previously named either Dd PK2 or Dd PK3 by different groups), predicts a 73-kDa polypeptide with 54% similarity to the c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00088a003

    authors： Anjard C,Etchebehere L,Pinaud S,Véron M,Reymond CD

    更新日期：1993-09-21 00:00:00

  • The red blood cell glucose transporter presents multiple, nucleotide-sensitive sugar exit sites.

    abstract：:At any instant, the human erythrocyte sugar transporter presents at least one sugar export site but multiple sugar import sites. The present study asks whether the transporter also presents more than one sugar exit site. We approached this question by analysis of binding of [3H]cytochalasin B (an export conformer liga...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015586w

    authors： Cloherty EK,Levine KB,Carruthers A

    更新日期：2001-12-25 00:00:00

  • The carboxyl-terminal region of factor IX is essential for its secretion.

    abstract：:The carboxyl-terminal region of factor IX (residues 403-415) contains several natural mutations which result in mild to severe forms of hemophilia B. A battery of factor IX minigene expression vectors carrying various mutations in the C-terminal region were constructed and studied by transient expression assay using H...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962002v

    authors： Kurachi S,Pantazatos DP,Kurachi K

    更新日期：1997-04-08 00:00:00

  • Protease-sensitive scrapie prion protein in aggregates of heterogeneous sizes.

    abstract：:The pathological prion protein PrP(Sc) is the only known component of the infectious prion. In cells infected with prions, PrP(Sc) is formed posttranslationally by the refolding of the benign cell surface glycoprotein PrP(C) into an aberrant conformation. The two PrP isoforms possess very different properties, as PrP(...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi025958g

    authors： Tzaban S,Friedlander G,Schonberger O,Horonchik L,Yedidia Y,Shaked G,Gabizon R,Taraboulos A

    更新日期：2002-10-22 00:00:00

  • Arginine 177 is involved in Mn(II) binding by manganese peroxidase.

    abstract：:Site-directed mutations R177A and R177K in the gene encoding manganese peroxidase isozyme 1 (mnp1) from Phanerochaete chrysosporium were generated. The mutant enzymes were expressed in P. chrysosporium during primary metabolic growth under the control of the glyceraldehyde-3-phosphate dehydrogenase gene promoter, puri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990943c

    authors： Sollewijn Gelpke MD,Moënne-Loccoz P,Gold MH

    更新日期：1999-08-31 00:00:00

  • Quantitative understanding of the energy transfer between fluorescent proteins connected via flexible peptide linkers.

    abstract：:The fusion of different protein domains via peptide linkers is a powerful, modular approach to obtain proteins with new functions. A detailed understanding of the conformational behavior of peptide linkers is important for applications such as fluorescence resonance energy transfer (FRET)-based sensor proteins and mul...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061288t

    authors： Evers TH,van Dongen EM,Faesen AC,Meijer EW,Merkx M

    更新日期：2006-11-07 00:00:00

  • Rat organic anion transporting protein 1A1 (Oatp1a1): purification and phosphopeptide assignment.

    abstract：:Rat organic anion transporting protein 1a1 (oatp1a1), a hepatocyte basolateral plasma membrane protein, mediates transport of various amphipathic compounds. Our previous studies indicated that serine phosphorylation of a single tryptic peptide inhibits its transport activity without changing its cell surface content. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi052437v

    authors： Xiao Y,Nieves E,Angeletti RH,Orr GA,Wolkoff AW

    更新日期：2006-03-14 00:00:00

  • Formation and rejoining of deoxyribonucleic acid double-strand breaks induced in isolated cell nuclei by antineoplastic intercalating agents.

    abstract：:The biochemical characteristics of the formation and disappearance of intercalator-induced DNA double-strand breaks (DSB) were studied in nuclei from mouse leukemia L1210 cells by using filter elution methodology [Bradley, M. O., & Kohn, K.W. (1979) Nucleic Acids Res. 7, 793-804]. The three intercalators used were 4'-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00309a013

    authors： Pommier Y,Schwartz RE,Kohn KW,Zwelling LA

    更新日期：1984-07-03 00:00:00

  • Subcellular distribution of glucocorticoid receptors in mouse fibroblasts.

    abstract：:Mouse fibroblasts contain a macromolecular binding component (receptor) which binds glucocorticoids specifically and with high affinity. This study shows that there are three different cellular forms of bound receptor and that it is experimentally possible to markedly alter the subcellular distribution of these three ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00672a030

    authors： Middlebrook JL,Wong MD,Ishii DN,Aronow L

    更新日期：1975-01-14 00:00:00

  • Isomers of epidermal growth factor with Ser --> Cys mutation at the N-terminal sequence: isomerization, stability, unfolding, refolding, and structure.

    abstract：:The structure of human epidermal growth factor (EGF, 53 amino acids) comprises three distinct loops (A, B, and C) connected correspondingly by the three native disulfide bonds, Cys(6)-Cys(20), Cys(14)-Cys(31), and Cys(33)-Cys(42). The connection of Cys(6) and Cys(20) forming the N-terminal A loop is essential for the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi051399c

    authors： Lu BY,Jiang C,Chang JY

    更新日期：2005-11-15 00:00:00

  • Interaction between SecA and SecYEG in micellar solution and formation of the membrane-inserted state.

    abstract：:Preprotein translocation in Escherichia coli is mediated by the translocase with SecA as peripheral ATPase and SecY, SecE, and SecG as membrane domain. To facilitate large-scale purification of the SecYEG heterotrimer, SecY was fused at its amino terminus with a hexahistidine tag and co-overexpressed with SecE and Sec...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972105t

    authors： van der Does C,Manting EH,Kaufmann A,Lutz M,Driessen AJ

    更新日期：1998-01-06 00:00:00

  • Base pairing in wheat germ ribosomal 5S RNA as measured by ultraviolet absorption, circular dichroism, and Fourier-transform infrared spectrometry.

    abstract：:Ultraviolet absorption (UV) and circular dichroism (CD) spectra of wheat germ 5S RNA, when compared to tRNAPhe, indicate a largely base-paired and base-stacked helical structure, containing up to 36 base pairs. Fourier-transform infrared (FT-IR) spectra of tRNAPhe and wheat germ ribosomal 5S RNA have been acquired at ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00311a012

    authors： Li SJ,Burkey KO,Luoma GA,Alben JO,Marshall AG

    更新日期：1984-07-31 00:00:00

  • Secretion of 35SO4-labeled proteins from isolated rat hepatocytes.

    abstract：:Sulfation is a Golgi-specific modification of secretory proteins. We have characterized the proteins that are labeled with 35SO4 in cultures of rat hepatocytes and studied their transport to the medium. Analysis by polyacrylamide gel electrophoresis showed that of the five most heavily labeled proteins, four had well-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00435a069

    authors： von Würtemberg MM,Fries E

    更新日期：1989-05-02 00:00:00

  • Computer Simulations Reveal Substrate Specificity of Glycosidic Bond Cleavage in Native and Mutant Human Purine Nucleoside Phosphorylase.

    abstract：:Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of purine ribonucleosides and 2'-deoxyribonucleosides, yielding the purine base and (2'-deoxy)ribose 1-phosphate as products. While this enzyme has been extensively studied, several questions with respect to the catalytic mechanism have rema...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b01347

    authors： Isaksen GV,Hopmann KH,Åqvist J,Brandsdal BO

    更新日期：2016-04-12 00:00:00

  • A conformational rearrangement in gramicidin A: from a double-stranded left-handed to a single-stranded right-handed helix.

    abstract：:A conformational transition is described for the polypeptide, gramicidin A, in which a dimer that forms a left-handed intertwined antiparallel helix is converted to a single-stranded amino terminus to amino terminus right-handed helix. The starting structure is determined here by solution NMR methods while reference i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00152a019

    authors： Zhang Z,Pascal SM,Cross TA

    更新日期：1992-09-22 00:00:00