当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Perturbation of tryptophan residues by point mutations in bacteriophage T4 lysozyme studied by optical detection of triplet-state magnetic resonance spectroscopy.

Perturbation of tryptophan residues by point mutations in bacteriophage T4 lysozyme studied by optical detection of triplet-state magnetic resonance spectroscopy.

Abstract：

:We have investigated perturbations of the triplet-state properties of Trp residues in bacteriophage T4 lysozyme caused by point mutations using low-temperature phosphorescence and optical detection of triplet-state magnetic resonance (ODMR) spectroscopy. Five temperature-sensitive mutants have been studied in detail. These include lysozymes with the point mutations Gln-105----Ala, Gln-105----Gly, Gln-105----Glu, Ala-146----Thr, and Trp-126----Gln. Changes in phosphorescence 0,0 band wavelength, intensity, the triplet-state zero-field splitting (ZFS), and the wavelength dependence of the ZFS were detected only from Trp-138 in each mutant. In the case of the Q105A mutation, the perturbations on Trp-138 have been ascribed to the combination of an increase in the polarizability of the environment and to the loss of hydrogen bonding of the enamine nitrogen of indole. For the Q105G mutation, we believe that Q is replaced by a solvent molecule in H bonding, leading to relatively small changes. In the Q105E mutation, the perturbation results largely from the introduction of a charged residue. In the case of the mutation A146T, the perturbation is associated with a local conformational change in which Trp-138 is shifted to a more solvent-exposed location. On the other hand, no significant spectroscopic changes in Trp-126 and Trp-158 were found in any of the mutants, suggesting that the perturbations are probably localized near Trp-138 for the mutations of positions 105 and 146. However, in the mutation W126Q, which occurs approximately 16 A away from Trp-138, significant changes of Trp-138 are detected, suggesting that the effects of this mutation are propagated over large distances.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Zang LH,Ghosh S,Maki AH

doi

10.1021/bi00431a041

subject

Has Abstract

pub_date

1989-03-07 00:00:00

pages

2245-51

issue

5

eissn

0006-2960

issn

1520-4995

journal_volume

28

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Structural, Dynamic, and Functional Characterization of a DnaX Mini-intein Derived from Spirulina platensis Provides Important Insights into Intein-Mediated Catalysis of Protein Splicing.

    abstract：:Protein splicing is a self-catalyzed post-translational modification in which the intein enzyme excises itself from a precursor protein and ligates the flanking sequences to produce a mature protein. We report the solution structure of a 136-residue DnaX mini-intein enzyme derived from the cyanobacterium Spirulina pla...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00828

    authors： Boral S,Maiti S,Basak AJ,Lee W,De S

    更新日期：2020-12-22 00:00:00

  • Stereospecific Radical-Mediated B12-Dependent Methyl Transfer by the Fosfomycin Biosynthesis Enzyme Fom3.

    abstract：:Fom3, the antepenultimate enzyme in the fosfomycin biosynthetic pathway in Streptomyces spp., is a class B cobalamin-dependent radical SAM methyltransferase that catalyzes methylation of (5'-cytidylyl)-2-hydroxyethylphosphonate (2-HEP-CMP) to form (5'-cytidylyl)-2-hydroxypropylphosphonate (2-HPP-CMP). Previously, the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00616

    authors： McLaughlin MI,van der Donk WA

    更新日期：2018-08-21 00:00:00

  • Effect of AMP on mRNA binding by yeast NAD+-specific isocitrate dehydrogenase.

    abstract：:Yeast mitochondrial NAD+-specific isocitrate dehydrogenase (IDH) has previously been shown to bind specifically to 5'-untranslated regions of yeast mitochondrial mRNAs, and transcripts containing these regions have been found to allosterically inhibit activity of the enzyme. This inhibition is relieved by AMP, an allo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0200662

    authors： Anderson SL,Schirf V,McAlister-Henn L

    更新日期：2002-06-04 00:00:00

  • Human Cytosolic and Mitochondrial Serine Hydroxymethyltransferase Isoforms in Comparison: Full Kinetic Characterization and Substrate Inhibition Properties.

    abstract：:Serine hydroxymethyltransferase (SHMT) catalyzes the reversible conversion of l-serine and tetrahydrofolate into glycine and 5,10-methylenetetrahydrofolate. This enzyme, which plays a pivotal role in one-carbon metabolism, is involved in cancer metabolic reprogramming and is a recognized target of chemotherapy interve...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b01074

    authors： Tramonti A,Nardella C,di Salvo ML,Barile A,Cutruzzolà F,Contestabile R

    更新日期：2018-12-26 00:00:00

  • FTIR study of the thermal denaturation of horseradish and cytochrome c peroxidases in D2O.

    abstract：:Fourier transform infrared (FTIR) spectroscopy was employed to examine the thermal denaturation of the Fe(III), Fe(II), and Fe(II)-CO forms of cytochrome c peroxidase and horseradish peroxidase in phosphate buffer at pD 7.0. The amide I' regions of the deconvolved spectra are consistent with predominantly alpha-helica...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952233m

    authors： Holzbaur IE,English AM,Ismail AA

    更新日期：1996-04-30 00:00:00

  • Disulfide-linked bovine beta-lactoglobulin dimers fold slowly, navigating a glassy folding landscape.

    abstract：:To gain insight into the folding of large proteins, we constructed a bovine beta-lactoglobulin (beta-lg) dimeric mutant, A34C/C121A beta-lg. In the mutant, a free thiol group of wild-type beta-lg at Cys121 was removed and two beta-lg molecules were linked by a disulfide bridge through Cys34 created at the dimer's inte...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi8001715

    authors： Yagi M,Kameda A,Sakurai K,Nishimura C,Goto Y

    更新日期：2008-06-03 00:00:00

  • Multidomain initiation factor 2 from Thermus thermophilus consists of the individual autonomous domains.

    abstract：:The three-dimensional chalice-like crystal structure of initiation factor 2 IF2/eIF5B from Methanobacterium thermoautotrophicum represents a novel fold and domain architecture in which the N-terminal G domain and the C-terminal C domain are separated by an approximately 40 A alpha-helix. Homologous Thermus thermophilu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi702295g

    authors： Zoldák G,Sedlák E,Wolfrum A,Musatov A,Fedunová D,Szkaradkiewicz K,Sprinzl M

    更新日期：2008-04-29 00:00:00

  • Association of insulin-like growth factor 2 with the insulin-linked polymorphic region in cultured fetal thymus cells.

    abstract：:The insulin-linked polymorphic region (ILPR) is a regulatory sequence in the promoter region upstream of the human insulin gene and is widely recognized as a locus of type 1 diabetes susceptibility. Polymorphism of the ILPR sequence can affect expression of both insulin and the adjacent insulin-like growth factor 2 (I...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900958x

    authors： Wang Y,Zhang H,Ligon LA,McGown LB

    更新日期：2009-09-01 00:00:00

  • Effect of chloride binding on the thermal trimer-monomer conversion of halorhodopsin in the solubilized system.

    abstract：:Halorhodopsin from Natronomonas pharaonis (NpHR) acts an inward-directed, light-driven chloride pump and forms a homotrimer. To evaluate effect of trimeric assembly, that is, intermolecular interaction, on the control or modulation of light-driven chloride pumping activity of individual HRs, it is important to underst...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901380c

    authors： Sasaki T,Aizawa T,Kamiya M,Kikukawa T,Kawano K,Kamo N,Demura M

    更新日期：2009-12-29 00:00:00

  • The acidic phosphoproteins from Saccharomyces cerevisiae ribosomes. NH2-terminal acetylation is a conserved difference between P1 and P2 proteins.

    abstract：:Isoelectrofocusing gels of acidic ribosomal proteins from most yeast strains reveal the presence of up to 10 bands which are the product of only 4 genes. The proteins have been characterized by NH2-terminal amino acid sequencing, specific antibodies, HPLC, and by taking advantage of acidic protein-defective yeast stra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00067a010

    authors： Santos C,Ortiz-Reyes B,Naranda T,Remacha M,Ballesta JP

    更新日期：1993-04-27 00:00:00

  • Redox properties of electron-transfer flavoprotein ubiquinone oxidoreductase as determined by EPR-spectroelectrochemistry.

    abstract：:We have determined the formal potential values for each electron transfer to electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO), in order to further characterize the thermodynamics of electron transport from various acyl-CoA thioesters to the mitochondrial ubiquinone pool. ETF-QO contains one [4Fe-4S]2+...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00162a012

    authors： Paulsen KE,Orville AM,Frerman FE,Lipscomb JD,Stankovich MT

    更新日期：1992-12-01 00:00:00

  • Stereochemical outcome and kinetic effects of Rp- and Sp-phosphorothioate substitutions at the cleavage site of vaccinia type I DNA topoisomerase.

    abstract：:To probe the mechanism of the reversible DNA phosphodiester bond cleavage and religation mechanism of the type I topoisomerase from vaccinia virus, we have synthesized DNA substrates carrying a single nonbridging Rp- or Sp-phosphorothioate (Ps) modification at the scissile phosphodiester (Pd) bond. Analysis of the ste...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992429c

    authors： Stivers JT,Jagadeesh GJ,Nawrot B,Stec WJ,Shuman S

    更新日期：2000-05-09 00:00:00

  • Solution conformation of the (-)-trans-anti-5-methylchrysene-dG adduct opposite dC in a DNA duplex: DNA bending associated with wedging of the methyl group of 5-methylchrysene to the 3'-side of the modification site.

    abstract：:This paper reports on NMR-molecular mechanics structural studies of the (-)-trans-anti-[MC]dG adduct positioned opposite dC in the sequence context of the d(C1-C2-A3-T4-C5-[MC]G6-C7-T8-A9-C10-C11).d(G12-G13-T14++ +-A15-G16-C17-G18- A19-T20-G21-G22) duplex [designated (-)-trans-anti-[MC]dG.dC 11-mer duplex]. This adduc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00018a029

    authors： Cosman M,Xu R,Hingerty BE,Amin S,Harvey RG,Geacintov NE,Broyde S,Patel DJ

    更新日期：1995-05-09 00:00:00

  • Conformational fluctuation of native-like and molten-globule-like cytochrome c observed by time-resolved hole burning.

    abstract：:Nanosecond to millisecond conformational fluctuations of Zn-substituted cytochrome c (ZnCytc) have been studied by the time-resolved transient hole-burning method. The investigation of low-temperature dynamics has been made on the ZnCytc solution sample in a water-glycerol mixture. The conformational fluctuations in t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981568b

    authors： Shibata Y,Takahashi H,Kaneko R,Kurita A,Kushida T

    更新日期：1999-02-09 00:00:00

  • Estrogen-induced changes in high-energy phosphate metabolism in rat uterus: 31P NMR studies.

    abstract：:Changes in the concentrations of high-energy phosphate metabolites were measured by 31P NMR spectroscopy of surviving rat uteri from 0-48 h following estrogen administration. Concentrations (millimoles per kilogram wet weight) of these metabolites in the untreated immature uterus, measured at 4 degrees C, were found t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00307a006

    authors： Degani H,Shaer A,Victor TA,Kaye AM

    更新日期：1984-06-05 00:00:00

  • Ultraviolet resonance Raman spectra of insulin and alpha-lactalbumin with 218- and 200-nm laser excitation.

    abstract：:Ultraviolet resonance Raman (RR) spectra, with 200- and 218-nm excitation from a H2-shifted quadrupled Nd:YAG laser, are reported for insulin and alpha-lactalbumin in dilute aqueous solution, at pH values known to produce differences in the exposure of the aromatic residues to solvent. At 200 nm, the spectra are domin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00329a009

    authors： Rava RP,Spiro TG

    更新日期：1985-04-09 00:00:00

  • Spatial proximity of the glycine-rich loop and the SH2 thiol in myosin subfragment 1.

    abstract：:Subfragment 1 (S1) prepared from rabbit skeletal muscle myosin was digested with trypsin to cleave the 95K heavy chain into three pieces, i.e., the 23K, 50K, and 20K fragments. The trypsin-treated S1 was then cross-linked with p-nitrophenyl iodoacetate. The cross-linker bridged one of the reactive thiols (SH2) in the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00408a045

    authors： Sutoh K,Hiratsuka T

    更新日期：1988-04-19 00:00:00

  • Factor IX activation by factor XIa proceeds without release of a free intermediate.

    abstract：:Factor IX activation by factor XIa is thought to proceed through the singly-cleaved free intermediate, factor IX alpha. However, we observed no intermediate development during factor IX activation by factor XIa when using a low substrate to enzyme ratio (44:1 mol/mol). This result can be explained by one of two mechan...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi962274y

    authors： Wolberg AS,Morris DP,Stafford DW

    更新日期：1997-04-08 00:00:00

  • Levuglandin forms adducts with histone h4 in a cyclooxygenase-2-dependent manner, altering its interaction with DNA.

    abstract：:Inflammation and subsequent cyclooxygenase-2 (COX-2) activity has long been linked with the development of cancer, although little is known about any epigenetic effects of COX-2. A product of COX-2 activation, levuglandin (LG) quickly forms covalent bonds with nearby primary amines, such as those in lysine, which lead...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi401673b

    authors： Carrier EJ,Zagol-Ikapitte I,Amarnath V,Boutaud O,Oates JA

    更新日期：2014-04-22 00:00:00

  • Conformation of valine side chains in ribonuclease T1 determined by NMR studies of homonuclear and heteronuclear 3J coupling constants.

    abstract：:A conformational analysis of the valine side chains of ribonuclease T1 (RNase T1) was performed using NMR spectroscopy, in particular homonuclear (1H, 1H and 13C, 13C) and heteronuclear (1H, 15N and 1H, 13C) vicinal spin-spin coupling constants as obtained from E.COSY-type NMR experiments. The coupling constants relat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00184a017

    authors： Karimi-Nejad Y,Schmidt JM,Rüterjans H,Schwalbe H,Greisinger C

    更新日期：1994-05-10 00:00:00

  • Structural Basis for Substrate and Oxygen Activation in Homoprotocatechuate 2,3-Dioxygenase: Roles of Conserved Active Site Histidine 200.

    abstract：:Kinetic and spectroscopic studies have shown that the conserved active site residue His200 of the extradiol ring-cleaving homoprotocatechuate 2,3-dioxygenase (FeHPCD) from Brevibacterium fuscum is critical for efficient catalysis. The roles played by this residue are probed here by analysis of the steady-state kinetic...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00709

    authors： Kovaleva EG,Rogers MS,Lipscomb JD

    更新日期：2015-09-01 00:00:00

  • Minor influence of sialic acid on conformation of a membrane-bound oligosaccharide recognition site.

    abstract：:Wideline 2H NMR spectroscopy was used to assess the conformational and orientational effects of N-acetylneuraminic acid (NeuAc) (sialic acid) as a component of a particular oligosaccharide chain at a bilayer membrane surface. For this purpose, three glycosphingolipids, sharing a neutral core tetrasaccharide and differ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952964m

    authors： Jones DH,Barber KR,Grant CW

    更新日期：1996-04-16 00:00:00

  • High-resolution structure of an HIV zinc fingerlike domain via a new NMR-based distance geometry approach.

    abstract：:A new method is described for determining molecular structures from NMR data. The approach utilizes 2D NOESY back-calculations to generate simulated spectra for structures obtained from distance geometry (DG) computations. Comparison of experimental and back-calculated spectra, including analysis of cross-peak buildup...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00454a005

    authors： Summers MF,South TL,Kim B,Hare DR

    更新日期：1990-01-16 00:00:00

  • Effect of flap modifications on human FEN1 cleavage.

    abstract：:The flap endonuclease, FEN1, plays a critical role in DNA replication and repair. Human FEN1 exhibits both a 5' to 3' exonucleolytic and a structure-specific endonucleolytic activity. On primer-template substrates containing an unannealed 5'-tail, or flap structure, FEN1 employs a unique mechanism to cleave at the poi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991321u

    authors： Bornarth CJ,Ranalli TA,Henricksen LA,Wahl AF,Bambara RA

    更新日期：1999-10-05 00:00:00

  • Active site of bee venom phospholipase A2: the role of histidine-34, aspartate-64 and tyrosine-87.

    abstract：:In bee venom phospholipase A2, histidine-34 probably functions as a Brønsted base to deprotonate the attacking water. Aspartate-64 and tyrosine-87 form a hydrogen bonding network with histidine-34. We have prepared mutants at these positions and studied their kinetic properties. The mutant in which histidine-34 is cha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9528412

    authors： Annand RR,Kontoyianni M,Penzotti JE,Dudler T,Lybrand TP,Gelb MH

    更新日期：1996-04-09 00:00:00

  • Is Arg418 the catalytic base required for the hydrolysis step of the IMP dehydrogenase reaction?

    abstract：:The first committed step of guanine nucleotide biosynthesis is the oxidation of inosine 5'-monophosphate (IMP) to xanthosine 5'-monophosphate (XMP) catalyzed by IMP dehydrogenase. The reaction involves the reduction of NAD(+) with the formation of a covalent enzyme intermediate (E-XMP). Hydrolysis of E-XMP requires th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi048342v

    authors： Guillén Schlippe YV,Hedstrom L

    更新日期：2005-09-06 00:00:00

  • Transposon Tn7 protein TnsD binding to Escherichia coli attTn7 DNA and its eukaryotic orthologs.

    abstract：:Transposon Tn7 inserts itself into the attTn7 target DNA sequence at the 3' end of the Escherichia coli glmS gene with high specificity and efficiency. This site in the E. coli genome displays amino acid conservation and nucleotide similarity with orthologous sequences in Archaebacteria and eukaryotes. On the basis of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035535u

    authors： Chakrabarti A,Desai P,Wickstrom E

    更新日期：2004-03-16 00:00:00

  • Mutations affecting the activity of toxic shock syndrome toxin-1.

    abstract：:Toxic shock syndrome toxin-1 (TSST-1), the potent staphylococcal exoprotein linked to most cases of the toxic shock syndrome, is a V beta-restricted T-cell mitogen (a so-called "superantigen"). TSST-ovine (TSST-O) is a natural variant of TSST-1, and is produced by certain ovine mastitis-associated strains of Staphyloc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00209a016

    authors： Deresiewicz RL,Woo J,Chan M,Finberg RW,Kasper DL

    更新日期：1994-11-01 00:00:00

  • Inhibition kinetics and emodin cocrystal structure of a type II polyketide ketoreductase.

    abstract：:Type II polyketides are a class of natural products that include pharmaceutically important aromatic compounds such as the antibiotic tetracycline and antitumor compound doxorubicin. The type II polyketide synthase (PKS) is a complex consisting of 5-10 standalone domains homologous to fatty acid synthase (FAS). Polyke...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7016427

    authors： Korman TP,Tan YH,Wong J,Luo R,Tsai SC

    更新日期：2008-02-19 00:00:00

  • Analysis of peptides for helical prediction.

    abstract：:Two terminally blocked peptides, acetylAETAAAKFLRQHMamide and acetylAETSSSRYLRQHMamide, were obtained by solid-phase synthesis, purified by reversed-phase chromatography, and characterized by fast atom bombardment mass spectrometry. Both peptides were soluble in aqueous solutions and remained monomeric over the concen...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00427a048

    authors： Merutka G,Stellwagen E

    更新日期：1989-01-10 00:00:00