High-resolution structure of an HIV zinc fingerlike domain via a new NMR-based distance geometry approach.

abstract：:In order to study in detail the generality of the NUX (N = A, U, G, or C; X = A, U, or C) rule for the GUC triplet adjacent to the cleavage site in hammerhead ribozymes, two kinetic parameters, namely, kcat and Km, were determined for substrates with mutations in this triplet, which included double mutants with mutati...

journal_title：Biochemistry

authors： Shimayama T,Nishikawa S,Taira K

更新日期：1995-03-21 00:00:00

abstract：:The reactivity of a series of para-substituted phenolic compounds in the peroxidation catalyzed by chloroperoxidase was investigated, and the results were interpreted on the basis of the binding characteristics of the substrates to the active site of the enzyme. Marked selectivity effects are observed. These operate t...

journal_title：Biochemistry

authors： Casella L,Poli S,Gullotti M,Selvaggini C,Beringhelli T,Marchesini A

更新日期：1994-05-31 00:00:00

abstract：:Optical detection of triplet-state magnetic resonance (ODMR) is employed to study the complexes formed between gene 32 protein (GP32), a single-stranded DNA-binding protein from bacteriophage T4, and the heavy-atom-derivatized polynucleotides poly(5-HgU) and poly(5-BrU). The triplet-state properties of some of the try...

journal_title：Biochemistry

authors： Khamis MI,Maki AH

更新日期：1986-10-07 00:00:00

abstract：:Prolyl endopeptidase (Prep) is a member of the prolyl peptidase family and is of interest because of its unique biochemistry and connections to cognitive function. Using an unbiased mass spectrometry (MS)-based peptidomics platform, we identified Prep-regulated peptides in the central nervous system (CNS) of mice by m...

journal_title：Biochemistry

authors： Nolte WM,Tagore DM,Lane WS,Saghatelian A

更新日期：2009-12-22 00:00:00

abstract：:Human corticosteroid-binding globulin (CBG) forms a dimer that was isolated by gel filtration, has full binding affinity and capacity, and can be dissociated to the monomer. Monomeric CBG consists of two distinct molecular variants, which were detected by polyacrylamide gel electrophoresis in the presence and absence ...

journal_title：Biochemistry

authors： Mickelson KE,Harding GB,Forsthoefel M,Westphal U

更新日期：1982-02-16 00:00:00

abstract：:Weak or nonexistent smallpox immunity in today's human population raises concerns about the possibility of natural or provoked genetic modifications leading to re-emergence of variola virus and other poxviruses. Thus, the development of new antiviral strategies aimed at poxvirus infections in humans is a high priority...

journal_title：Biochemistry

authors： Duraffour S,Ishchenko AA,Saparbaev M,Crance JM,Garin D

更新日期：2007-10-23 00:00:00

abstract：:Diethyl pyrocarbonate (DEP) is an electrophilic reagent that is used to modify reversibly the histidine residues of proteins. Unfortunately, the lability of the acylated histidine adduct usually does not permit the isolation and identification of the modified histidine. By use of 500-MHz proton NMR spectroscopy, it ha...

journal_title：Biochemistry

authors： Altman J,Lipka JJ,Kuntz I,Waskell L

更新日期：1989-09-19 00:00:00

abstract：:The effect of pretreatment with sodium butyrate on DNA excision repair was studied in intact and permeable confluent (i.e., growth-inhibited) diploid human fibroblasts. Exposure to 20 mM sodium butyrate for 48 h increased subsequent ultraviolet (UV)-induced [methyl-3H]thymidine incorporation by intact AG1518 fibroblas...

journal_title：Biochemistry

authors： Dresler SL

更新日期：1985-11-19 00:00:00

abstract：:The X-ray crystal structures of Escherichia coli gamma-aminobutyrate aminotransferase unbound and bound to the inhibitor aminooxyacetate are reported. The enzyme crystallizes from ammonium sulfate solutions in the P3(2)21 space group with a tetramer in the asymmetric unit. Diffraction data were collected to 2.4 A reso...

journal_title：Biochemistry

authors： Liu W,Peterson PE,Carter RJ,Zhou X,Langston JA,Fisher AJ,Toney MD

更新日期：2004-08-31 00:00:00

abstract：:A new type of thrombin exo-site inhibitor has been designed with enhanced inhibitory potency and increased metabolic stability. With the aid of the model of the structure of the thrombin-hirudin fragment complex [Yue, S.-Y., DiMaio, J., Szewczuk, Z., Purisima, E. O., Ni, F., & Konishi, Y. (1992) Protein Eng. 5, 77-85]...

journal_title：Biochemistry

authors： Szewczuk Z,Gibbs BF,Yue SY,Purisima EO,Konishi Y

更新日期：1992-09-29 00:00:00

abstract：:Cell-free extracts from the HTl clone of cultured Chinese hamster lung cells efficiently promote the incorporation of proline into newly synthesized material, 50% of which is digestible to small peptides by highly purified bacterial collagenase. The synthesis of the these products occurs under optimal protein synthesi...

journal_title：Biochemistry

authors： Haralson MA,Frey KL,Mitchell WM

更新日期：1978-03-07 00:00:00

abstract：:Decarboxylated S-adenosylmethionine was found to be a substrate for the nuclear acetyltransferases that act on polyamines and on histones. The rate of acetylation of decarboxylated S-adenosylmethionine was more than twice that of spermidine at saturating substrate concentrations, and decarboxylated S-adenosylmethionin...

journal_title：Biochemistry

authors： Pegg AE,Wechter RS,Clark RS,Wiest L,Erwin BG

更新日期：1986-01-28 00:00:00

abstract：:Carbamoyl phosphate synthetase from E. coli catalyzes the synthesis of carbamoyl phosphate through a series of four reactions occurring at three active sites connected by a molecular tunnel of 100 A. To understand the mechanism for coordination and synchronization among the active sites, the pre-steady-state time cour...

journal_title：Biochemistry

authors： Miles BW,Raushel FM

更新日期：2000-05-02 00:00:00

abstract：:High-resolution 2D NMR spectra of the duplex CGCGAATTCGCG with deoxyribose sugars but with the normal phosphodiester linker replaced by an N3' --> P5' phosphoramidate (NP) group have been used to establish a solution structure for the duplex. Distance, angle, and base pair hydrogen-bonding constraints were used to ref...

journal_title：Biochemistry

authors： Ding D,Gryaznov SM,Wilson WD

更新日期：1998-09-01 00:00:00

abstract：:Replacement of methionine (Met) residues by selenomethionine (SeMet) was recently shown to facilitate the crystallographic analysis of protein structure through the application of multi-wavelength anomalous diffraction techniques [Yang et al. (1990) Science (Washington, D.C.) 249, 1398-1405]. The availability of SeMet...

journal_title：Biochemistry

authors： Boles JO,Cisneros RJ,Weir MS,Odom JD,Villafranca JE,Dunlap RB

更新日期：1991-11-19 00:00:00

abstract：:Evidence of nonequilibrium local heating in transient spectra of LHCII, the main light-harvesting complex of plants, was studied by using various excitation intensities over a wide temperature range, from 10 K to room temperature. No obvious manifestation of local heating was found at room temperature, whereas at 10 K...

journal_title：Biochemistry

authors： Gulbinas V,Karpicz R,Garab G,Valkunas L

更新日期：2006-08-08 00:00:00

abstract：:Two extremely potent inhibitors of citrate synthase, carboxyl and primary amide analogues of acetyl coenzyme A, have been synthesized. The ternary complexes of these inhibitors with oxaloacetate and citrate synthase have been crystallized and their structures analyzed at 1.70- and 1.65-A resolution, respectively. The ...

journal_title：Biochemistry

authors： Usher KC,Remington SJ,Martin DP,Drueckhammer DG

更新日期：1994-06-28 00:00:00

abstract：:A hallmark of penicillin-binding protein 2 (PBP2) from penicillin-resistant strains of Neisseria gonorrhoeae is insertion of an aspartate after position 345. The insertion resides on a loop near the active site and is immediately adjacent to an existing aspartate (Asp346) that forms a functionally important hydrogen b...

journal_title：Biochemistry

authors： Fedarovich A,Cook E,Tomberg J,Nicholas RA,Davies C

更新日期：2014-12-09 00:00:00

abstract：:Changes in the concentrations of high-energy phosphate metabolites were measured by 31P NMR spectroscopy of surviving rat uteri from 0-48 h following estrogen administration. Concentrations (millimoles per kilogram wet weight) of these metabolites in the untreated immature uterus, measured at 4 degrees C, were found t...

journal_title：Biochemistry

authors： Degani H,Shaer A,Victor TA,Kaye AM

更新日期：1984-06-05 00:00:00

abstract：:Photolyzed rhodopsin is phosphorylated at multiple serine and threonine residues during the quenching of phototransduction. Sites of phosphorylation by rhodopsin kinase have been localized to the C-terminal region of rhodopsin, but no information was available on the kinetics and identity of phosphorylated residues. T...

journal_title：Biochemistry

authors： Ohguro H,Palczewski K,Ericsson LH,Walsh KA,Johnson RS

更新日期：1993-06-01 00:00:00

abstract：:The gene encoding enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system from an Escherichia coli enzyme I mutant was cloned and sequenced. The mutation was shown to be a guanine to adenine transition resulting in an altered protein in which glycine-338 was replaced by aspartic acid. The enzyme I structur...

journal_title：Biochemistry

authors： Seok YJ,Lee BR,Gazdar C,Svenson I,Yadla N,Peterkofsky A

更新日期：1996-01-09 00:00:00

abstract：:Frequency-resolved fluorescence measurements have been performed to quantitate the lateral stress of the lipid layer containing nonbilayer phase preferring dioleoylphosphatidylethanolamine (DOPE). On the basis of a new rotational diffusion model, the wobbling diffusion constant (Dw), the curvature-related hopping diff...

journal_title：Biochemistry

authors： Chen SY,Cheng KH,Van der Meer BW

更新日期：1992-04-21 00:00:00

abstract：:The N-acyl- l-homoserine lactone hydrolases (AHL lactonases) have attracted considerable attention because of their ability to quench AHL-mediated quorum-sensing pathways in Gram-negative bacteria and because of their relation to other enzymes in the metallo-beta-lactamase superfamily. To elucidate the detailed cataly...

journal_title：Biochemistry

authors： Momb J,Wang C,Liu D,Thomas PW,Petsko GA,Guo H,Ringe D,Fast W

更新日期：2008-07-22 00:00:00

abstract：:Ner repressors of the transposable phages Mu and D108 play a central role in regulating the expression of the early (transposase) operon and in ensuring that phage growth proceeds along a lytic pathway. The latter function is analogous to that performed by the Cro protein of phage lambda. Unlike lambda Cro, however, t...

journal_title：Biochemistry

authors： Benevides JM,Kukolj G,Autexier C,Aubrey KL,DuBow MS,Thomas GJ Jr

更新日期：1994-09-06 00:00:00

abstract：:The site-directed mutagenesis of a number of proposed active site residues of 5-enolpyruvyl shikimate-3-phosphate (EPSP) synthase is reported. Several of these mutations resulted in complete loss of enzyme activity indicating that these residues are probably involved with catalysis, notably K22R, K411R, D384A, R27A, R...

journal_title：Biochemistry

authors： Shuttleworth WA,Pohl ME,Helms GL,Jakeman DL,Evans JN

更新日期：1999-01-05 00:00:00

abstract：:The structural and motional properties of membrane lipids in various physical states and macroscopic organizations are elucidated by 1H NMR dipolar echo decay spectroscopy (DECODE). Multilamellar lipid dispersions in the gel (L beta') and liquid-crystalline (L alpha) states and a nonbilayer, hexagonal (HII) phase are ...

journal_title：Biochemistry

authors： Janes N,Rubin E,Taraschi TF

更新日期：1990-09-11 00:00:00

abstract：:Interaction of von Willebrand factor (vWF) with its platelet receptor only occurs in vitro in the presence of a modulator such as ristocetin. We have recently confirmed that the human platelet membrane glycoprotein (GP) Ib-IX complex is the receptor involved in the ristocetin-dependent binding of vWF by reconstitution...

journal_title：Biochemistry

authors： Andrews RK,Booth WJ,Gorman JJ,Castaldi PA,Berndt MC

更新日期：1989-10-17 00:00:00

abstract：:Actin depolymerizing factor (ADF)/cofilin enhances turnover of actin filaments by severing and depolymerizing filaments. A number of proteins functionally interact with ADF/cofilin to modulate the dynamics of actin filaments. Actin-interacting protein 1 (AIP1) has emerged as a conserved WD-repeat protein that specific...

journal_title：Biochemistry

authors： Ono S

更新日期：2003-11-25 00:00:00

abstract：:We reported previously that cdc2 kinase decreased the microtubule-stabilizing ability of a major HeLa cell microtubule-associated protein, MAP4, by phosphorylation in vitro [Ookata, K., et al. (1995) J. Cell Biol. 128, 849-862]. An important question raised by this study is whether MAP4 is indeed phosphorylated by cdc...

journal_title：Biochemistry

authors： Ookata K,Hisanaga S,Sugita M,Okuyama A,Murofushi H,Kitazawa H,Chari S,Bulinski JC,Kishimoto T

更新日期：1997-12-16 00:00:00

abstract：:The Raman spectra of the nonbridging V--O bonds in the myosin S1.MgADP.Vi complex, often believed to be a transition-state analogue for the phosphotransfer reaction catalyzed by myosin, and in a vanadate solution model compound have been obtained using Raman difference spectroscopic techniques. A symmetric/asymmetric ...

journal_title：Biochemistry

authors： Deng H,Wang J,Callender RH,Grammer JC,Yount RG

更新日期：1998-08-04 00:00:00