当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Kinetic studies on the mechanism of pepsin action.

Kinetic studies on the mechanism of pepsin action.

Abstract：

:The linear noncompetitive inhibition of the pepsin-catalyzed hydrolysis of Ac-Phe-Phe-Gly at pH 2.1 by L-Ac-Phe, L-Ac-Phe-NH2, and L-Ac-Phe-OEt has been claimed to substantiate the ordered release of products specified by the amino-enzyme mechanism for pepsin action. According to this interpretation, the binding of inhibitor to free enzyme and the amino-enzyme intermediate (Scheme I) generates the observed inhibition pattern. The proposition is valid only if a simple alternative explanation for the kinetic data, Scheme II, can be disproved. Scheme II attributes the inhibition pattern to the binding of inhibitor to free enzyme and the enzyme-substrate (Michaelis) complex. The experiments reported here have enabled us to distinguish between the two mechanisms. The pepsin-catalyzed hydrolyses of Ac-Phe-Trp, Z-H'IS-Phe-Trp, Z-Gly-His-Phe-Trp, and Z-Ala-His-Phe-Trp at pH 1.8 occur exclusively at the Phe-Trp bond and must yield the same amino-enzyme, E-Trp, if it is implicated. Under these circumstances, Scheme I requires that a plot of 1/kc vs. (I)o for the four substrates and a given noncompetitive inhibitor provide a set of four parallel lines. Scheme II predicts that the four lines generally will not be parallel. L-Ac-Phe, L-Ac-Phe-NH2, L-Ac-Phe-OMe, and D-Ac-Phe act as linear noncompetitive inhibitors for the pepsin-catalyzed hydrolysis of the four Trp-containing substrates. The plot of 1/kc vs. (I)o for each inhibitor results in a set of four nonparallel lines. Therefore Scheme II must be correct and the detection of noncompetitive inhibition accompanying the pepsin-catalyzed hydrolysis of peptides offers no insight into the merits of the amino-enzyme hypothesis.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Silver MS,Stoddard M

doi

10.1021/bi00674a023

subject

Has Abstract

pub_date

1975-02-11 00:00:00

pages

614-21

issue

3

eissn

0006-2960

issn

1520-4995

journal_volume

14

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Catalytic acid-base groups in yeast pyruvate decarboxylase. 1. Site-directed mutagenesis and steady-state kinetic studies on the enzyme with the D28A, H114F, H115F, and E477Q substitutions.

    abstract：:The roles of four of the active center groups with potential acid-base properties in the region of pH optimum of pyruvate decarboxylase from Saccharomyces cerevisiae have been studied with the substitutions Asp28Ala, His114Phe, His115Phe, and Glu477Gln, introduced by site-directed mutagenesis methods. The steady-state...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002855u

    authors： Liu M,Sergienko EA,Guo F,Wang J,Tittmann K,Hübner G,Furey W,Jordan F

    更新日期：2001-06-26 00:00:00

  • Identification of a nucleoside triphosphate binding site on calf thymus RNA polymerase II.

    abstract：:A nucleoside triphosphate binding site on calf thymus RNA polymerase II was identified by using photoaffinity analogues of adenosine 5'-triphosphate and guanosine 5'-triphosphate. Both radiolabeled 8-azidoadenosine 5'-triphosphate (8-N3ATP) and radiolabeled 8-azidoguanosine 5'-triphosphate (8-N3GTP) bound to a single ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00349a038

    authors： Freund E,McGuire PM

    更新日期：1986-01-14 00:00:00

  • ATP cross-linked to Escherichia coli single-strand DNA-binding protein can be utilized by the catalytic center of primase as initiating nucleotide for primer RNA synthesis on phage G4oric template.

    abstract：:We report a new observation of the role of Escherichia coli single-strand DNA binding protein (SSB) in synthesis of primer RNA (pRNA) catalyzed by.E.coli primase on the SSB-coated phage G4oric template. Using a set of ATP priming substrates with reactive groups attached to the 5' gamma-phosphate on different length "a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972455f

    authors： Godson GN,Mustaev AA,Sun W

    更新日期：1998-03-17 00:00:00

  • Structural Analysis Provides Mechanistic Insight into Nicotine Oxidoreductase from Pseudomonas putida.

    abstract：:The first structure of nicotine oxidoreductase (NicA2) was determined by X-ray crystallography. Pseudomonas putida has evolved nicotine-degrading activity to provide a source of carbon and nitrogen. The structure establishes NicA2 as a member of the monoamine oxidase family. Residues 1-50 are disordered and may play a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00963

    authors： Tararina MA,Janda KD,Allen KN

    更新日期：2016-12-06 00:00:00

  • Transient kinetics of redox reactions of flavodoxin: effects of chemical modification of the flavin mononucleotide prosthetic group on the dynamics of intermediate complex formation and electron transfer.

    abstract：:The effects of structural modifications of the flavin mononucleotide (FMN) prosthetic group of Clostridium pasteurianum flavodoxin on the kinetics of electron transfer to the oxidized form (from 5-deazariboflavin semiquinone produced by laser flash photolysis) and from the semiquinone form (to horse heart cytochrome c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00281a034

    authors： Simondsen RP,Tollin G

    更新日期：1983-06-07 00:00:00

  • Formation of a copper specific binding site in non-native states of beta-2-microglobulin.

    abstract：:A debilitating complication of long-term hemodialysis is the deposition of beta-2-microglobulin (beta2m) as amyloid plaques in the joint space. We have recently shown that Cu(2+) can be a contributing, if not causal, factor at concentrations encountered during dialysis therapy. The basis for this effect is destabiliza...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi025944a

    authors： Eakin CM,Knight JD,Morgan CJ,Gelfand MA,Miranker AD

    更新日期：2002-08-27 00:00:00

  • Blue-light-induced unfolding of the Jα helix allows for the dimerization of aureochrome-LOV from the diatom Phaeodactylum tricornutum.

    abstract：:Aureochromes have recently been shown to act as blue-light-regulated transcription factors in the stramenopile alga Vaucheria frigida. They comprise a light-, oxygen-, or voltage-sensitive (LOV) domain as a sensory module with flavin mononucleotide (FMN) as a chromophore and a basic region leucine zipper (bZIP) domain...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi400197u

    authors： Herman E,Sachse M,Kroth PG,Kottke T

    更新日期：2013-05-07 00:00:00

  • Assignment of spectral substructures to pigment-binding sites in higher plant light-harvesting complex LHC-II.

    abstract：:The trimeric main light-harvesting complex (LHC-II) is the only antenna complex of higher plants of which a high-resolution 3D structure has been obtained (Kühlbrandt, W., Wang, D., and Fujiyoshi, Y. (1994) Nature 367, 614-621) and which can be refolded in vitro from its components. Four different recombinant forms of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015875k

    authors： Rogl H,Schödel R,Lokstein H,Kühlbrandt W,Schubert A

    更新日期：2002-02-19 00:00:00

  • Contribution of light chain residues to high affinity binding in an HIV-1 antibody explored by combinatorial scanning mutagenesis.

    abstract：:Detailed analysis of factors governing high affinity antibody-antigen interactions yields important insight into molecular recognition and facilitates the design of functional antibody libraries. Here we describe comprehensive mutagenesis of the light chain complementarity determining regions (CDRs) of HIV-1 antibody ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100293q

    authors： Da Silva GF,Harrison JS,Lai JR

    更新日期：2010-07-06 00:00:00

  • Cyclic peptides incorporating 4-carboxyphenylalanine and phosphotyrosine are potent inhibitors of pp60(c-)(src).

    abstract：:The protein tyrosine kinase, pp60(c-)(src), is involved in cellular signaling and is activated during mitosis and in various tumors. We have been employing cyclic decapeptides to identify the determinants for substrate binding and phosphorylation to develop inhibitors competitive with protein substrates of Src. A stru...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi992994f

    authors： Wang W,Ramdas L,Sun G,Ke S,Obeyesekere NU,Budde RJ,McMurray JS

    更新日期：2000-05-02 00:00:00

  • Transient spectroscopy of bacterial rhodopsins with an optical multichannel analyzer. 2. Effects of anions on the halorhodopsin photocycle.

    abstract：:We find that the photocycle of halorhodopsin (HR) in the presence of nitrate (but not chloride) consists of two parallel series of reactions. The first is essentially the same as that which occurs in the presence of chloride: HRhv----HRK----HRKL----HRL----HRO----HR. The second photocycle, however, which we describe as...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00438a039

    authors： Zimányi L,Lanyi JK

    更新日期：1989-06-13 00:00:00

  • In vitro protein kinase C phosphorylation sites of placental lipocortin.

    abstract：:Human placental lipocortin is a high-affinity substrate for rat brain protein kinase C in vitro with phosphorylation occurring on serine and threonine residues in a ratio of approximately 2 to 1. Comparison of the ability of various N-terminal-truncated derivatives of lipocortin to serve as phosphorylation substrates,...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00412a008

    authors： Schlaepfer DD,Haigler HT

    更新日期：1988-06-14 00:00:00

  • Analysis of DNA-dependent protein kinase-mediated DNA end joining by two-photon fluorescence cross-correlation spectroscopy.

    abstract：:Nonhomologous end joining (NHEJ) is the primary mechanism by which mammalian cells repair DNA double-strand breaks (DSBs). Proteins known to play a role in NHEJ include the DNA-dependent protein kinase catalytic subunit (DNA-PKcs), the Ku 70/Ku 80 heterodimer (Ku), XRCC4, and DNA ligase IV. One of the main roles of th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0524060

    authors： Merkle D,Block WD,Yu Y,Lees-Miller SP,Cramb DT

    更新日期：2006-04-04 00:00:00

  • The structure of human parathyroid hormone from a study of fragments in solution using 1H NMR spectroscopy and its biological implications.

    abstract：:In order to gain insight into the structure of human parathyroid hormone (hPTH), four fragments [hPTH(1-34), hPTH(18-48), hPTH(28-48), and hPTH(53-84)], which cover all regions of the intact hormone, have been investigated by CD and NMR spectroscopy in combination with distance geometry, and restrained molecular dynam...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00173a010

    authors： Wray V,Federau T,Gronwald W,Mayer H,Schomburg D,Tegge W,Wingender E

    更新日期：1994-02-22 00:00:00

  • Accumulation of immunoglobulin messenger ribonucleic acid in immunized mouse spleen.

    abstract：:We have measured the concentration of mRNAs coding for immunoglobulins, k and lambda type light chains and gamma 1 type heavy chain, in mouse spleen cells activated by bacterial lipopolysaccharide or sheep red blood cells. These mRNAs were quantitated by hybridization to radioactive DNA complementary to highly purifie...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00645a018

    authors： Honjo T,Shimizu A,Tsuda M,Natori S,Katatoka T,Dohmoto C,Mano Y

    更新日期：1977-12-27 00:00:00

  • Kinetic mechanism of human histone acetyltransferase P/CAF.

    abstract：:Human transcriptional coactivator P/CAF (p300/CBP-associating factor) is a histone acetyltransferase (HAT) and is a member of the GNAT (GCN5 related N-acetyltransferases) superfamily. P/CAF was originally identified by its ability to activate transcription of a variety of genes through its interaction with p300/CBP. T...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi001272h

    authors： Tanner KG,Langer MR,Denu JM

    更新日期：2000-10-03 00:00:00

  • Calcium-modulated guanylate cyclase transduction machinery in the photoreceptor--bipolar synaptic region.

    abstract：:Rod outer segment membrane guanylate cyclase (ROS-GC) transduction system is a central component of the Ca(2+)-sensitive phototransduction machinery. The system is composed of two parts: Ca(2+) sensor guanylate cyclase activating protein (GCAP) and ROS-GC. GCAP senses Ca(2+) impulses and inhibits the cyclase. This ope...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034025x

    authors： Venkataraman V,Duda T,Vardi N,Koch KW,Sharma RK

    更新日期：2003-05-20 00:00:00

  • Arginine vasopressin inhibition of cyclin D1 gene expression blocks the cell cycle and cell proliferation in the mouse Y1 adrenocortical tumor cell line.

    abstract：:Arginine vasopressin (AVP) is a nonapeptide long known as an endocrine and paracrine regulator of important systemic functions, namely, vasoconstriction, gluconeogenesis, corticosteroidogenesis, and excretion of water and urea. Here we report, for the first time, that AVP specifically inhibits expression of the cyclin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026807g

    authors： Schwindt TT,Forti FL,Juliano MA,Juliano L,Armelin HA

    更新日期：2003-02-25 00:00:00

  • Cytochrome P-450 inactivation by 3-alkylsydnones. Mechanistic implications of N-alkyl and N-alkenyl heme adduct formation.

    abstract：:Incubation of 3-(2-phenylethyl)-4-methylsydnone (PMS) with liver microsomes from phenobarbital-pretreated rats or with reconstituted cytochrome P-450b results in loss of the enzyme chromophore. Chromophore loss is NADPH-dependent even though the sydnone decomposes by an oxygen- but not enzyme-dependent process to give...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00413a034

    authors： Grab LA,Swanson BA,Ortiz de Montellano PR

    更新日期：1988-06-28 00:00:00

  • Solution structure and lipid membrane partitioning of VSTx1, an inhibitor of the KvAP potassium channel.

    abstract：:VSTx1 is a voltage sensor toxin from the spider Grammostola spatulata that inhibits KvAP, an archeabacterial voltage-activated K(+) channel whose X-ray structure has been reported. Although the receptor for VSTx1 and the mechanism of inhibition are unknown, the sequence of the toxin is related to hanatoxin (HaTx) and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0477034

    authors： Jung HJ,Lee JY,Kim SH,Eu YJ,Shin SY,Milescu M,Swartz KJ,Kim JI

    更新日期：2005-04-26 00:00:00

  • Investigation of the shape and size of myosin subfragment 1 using small-angle X-ray scattering.

    abstract：:Measurement of x-ray scattering at very small angles by solutions of myosin subfragment 1 (S1) yields a radius of gyration of 3.24 nm (mean) +/- 0.03 nm (standard error for N = 9). If S1 is assumed to be ellipsoid of revolution, of uniform electron density, with a molecular weight of 1.15 X 10(5) and a partial specifi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00605a009

    authors： Kretzschmar KM,Mendelson RA,Morales MF

    更新日期：1978-06-13 00:00:00

  • Purification and characterization of a gamma-like DNA polymerase from Chlamydomonas reinhardtii.

    abstract：:A crude in vitro system which initiates chloroplast DNA synthesis near the D-loop site mapped by electron microscopy [Wu, M., Lou, J. K., Chang, D. Y., Chang, C. H., & Nie, Z. Q. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 6761-6765] consists of soluble proteins and proteins extracted from purified thylakoid membrane. In...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00218a034

    authors： Wang ZF,Yang J,Nie ZQ,Wu M

    更新日期：1991-01-29 00:00:00

  • Use of cytochrome P-450scc to measure cholesterol-lipid interactions.

    abstract：:The interaction of cholesterol with phospholipids has been studied with a variety of techniques; however, the possible consequences of such interactions in vivo have not been demonstrated. In this study, the cholesterol-dependent absorbance spectrum of cytochrome P-450scc was used to monitor cholesterol availability i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00363a017

    authors： Stevens VL,Lambeth JD,Merrill AH Jr

    更新日期：1986-07-29 00:00:00

  • A cluster of aromatic residues in the sixth membrane-spanning segment of the dopamine D2 receptor is accessible in the binding-site crevice.

    abstract：:The binding site of the dopamine D2 receptor, like that of other homologous G protein-coupled receptors, is contained within a water-accessible crevice formed among its seven membrane-spanning segments. Using the substituted-cysteine accessibility method, we previously mapped the residues in the third, fifth, and seve...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972241y

    authors： Javitch JA,Ballesteros JA,Weinstein H,Chen J

    更新日期：1998-01-27 00:00:00

  • A major proportion of N-glycoproteins are transiently glucosylated in the endoplasmic reticulum.

    abstract：:N-linked, high-mannose-type oligosaccharides lacking glucose residues may be transiently glucosylated directly from UDP-Glc in the endoplasmic reticulum of mammalian, plant, fungal, and protozoan cells. The products formed have been identified as N-linked Glc1Man5-9GlcNAc2 and glucosidase II is apparently the enzyme r...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00226a017

    authors： Gañán S,Cazzulo JJ,Parodi AJ

    更新日期：1991-03-26 00:00:00

  • Spectroscopic and kinetic studies of lipases solubilized in reverse micelles.

    abstract：:The conformation and activity of three different lipases have been studied in reverse micelles formed by sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. In the case of human pancreatic lipase, the conformation of the polypeptide chain--as judged from far-UV circular dichroism measurements--is only slightly...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00066a025

    authors： Walde P,Han D,Luisi PL

    更新日期：1993-04-20 00:00:00

  • Hydrogen bonding at the active site of delta 5-3-ketosteroid isomerase.

    abstract：:The solution secondary structure of the highly active Y55F/Y88F "Tyr-14-only" mutant of delta 5-3-ketosteroid isomerase complexed with 19-nortestosterone hemisuccinate has been shown to consist of three helices, a six-stranded mixed beta-sheet, and five turns. The steroid binds near the general acid, Tyr-14, on helix ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971549m

    authors： Zhao Q,Abeygunawardana C,Gittis AG,Mildvan AS

    更新日期：1997-12-02 00:00:00

  • Kinetics and mechanism of transitions involving the lamellar, cubic, inverted hexagonal, and fluid isotropic phases of hydrated monoacylglycerides monitored by time-resolved X-ray diffraction.

    abstract：:A study of the dynamics and mechanism of the various thermotropic phase transitions undergone by the hydrated monoacylglycerides monoolein and monoelaidin, in the temperature range of 20-120 degrees C and from 0 to 5 M NaCl, has been undertaken. Measurements were made by using time-resolved X-ray diffraction at the Co...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00394a008

    authors： Caffrey M

    更新日期：1987-10-06 00:00:00

  • Molecular basis of reduced albumin synthesis in Morris hepatoma 7777.

    abstract：:The level of albumin mRNA in the normal Buffalo rat liver and Morris hepatoma 7777 was compared by molecular hybridization with albumin complementary DNA (cDNA) and translational assays. Albumin mRNA was found to be 10% of the total rat liver poly(A)-containing RNA population but reduced approximately fourfold in the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00608a028

    authors： Tse TP,Morris HP,Taylor JM

    更新日期：1978-07-25 00:00:00

  • Acylation of lysine 860 allows tight binding and cytotoxicity of Bordetella adenylate cyclase on CD11b-expressing cells.

    abstract：:The Bordetella adenylate cyclase toxin-hemolysin (CyaA, ACT, or AC-Hly) forms cation-selective membrane channels and delivers into the cytosol of target cells an adenylate cyclase domain (AC) that catalyzes uncontrolled conversion of cellular ATP to cAMP. Both toxin activities were previously shown to depend on post-t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050459b

    authors： Masin J,Basler M,Knapp O,El-Azami-El-Idrissi M,Maier E,Konopasek I,Benz R,Leclerc C,Sebo P

    更新日期：2005-09-27 00:00:00