Abstract:
:The interaction of cholesterol with phospholipids has been studied with a variety of techniques; however, the possible consequences of such interactions in vivo have not been demonstrated. In this study, the cholesterol-dependent absorbance spectrum of cytochrome P-450scc was used to monitor cholesterol availability in both micellar and vesicular environments. By use of this approach, in conjunction with titration of putative cholesterol binding species, a tight, approximately equimolar complex of cholesterol and digitonin was demonstrated. Sphingomyelin (SM) (both the synthetic N-palmitoyl and bovine brain forms) gave sigmoidal titration curves, suggesting a cooperative interaction between this lipid and cholesterol. The interaction of bovine brain glycerolipids and cholesterol was weaker than that of SM and showed no cooperativity. The importance of the phospholipid head group in these interactions was established by the differences in the ability of synthetic 1-palmitoyl-2-oleoylphosphatidylcholine, -phosphatidylethanolamine, and -phosphatidylserine to affect cholesterol availability. Comparison of these results with those of the bovine brain phospholipids indicates that the acyl chain composition of these molecules is also important to these interactions. Titrations of SM in phospholipid vesicles containing cytochrome P-450scc and different types of phosphatidylcholine established that the SM-cholesterol interactions also occur in a bilayer membrane. This study demonstrates that the association of cholesterol with cytochrome P-450scc is inhibited by concentrations of SM commonly found in biological membranes. Therefore, such cholesterol-lipid interactions can potentially affect the function of membrane enzymes.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Stevens VL,Lambeth JD,Merrill AH Jrdoi
10.1021/bi00363a017subject
Has Abstractpub_date
1986-07-29 00:00:00pages
4287-92issue
15eissn
0006-2960issn
1520-4995journal_volume
25pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Chemosensory cilia of olfactory receptor neurons contain an adenylate cyclase which is stimulated by high concentrations of odorants. Cyclic AMP produced by this enzyme has been proposed to act as second messenger in olfactory transduction. Here we report that olfactory cilia contain calmodulin and that calmodulin pot...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00469a004
更新日期:1990-05-01 00:00:00
abstract::The actin depolymerizing factor (ADF)/cofilins are an essential group of proteins that are important regulators of actin filament turnover in vivo. Although protists and yeasts express only a single member of this family, metazoans express two or more members in many cell types. In cells expressing both ADF and cofili...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049797n
更新日期:2004-06-08 00:00:00
abstract::The conformation of the trans-anti-(1S,2R,3S,4R)-N(2)-[1-(1,2,3,4-tetrahydro-2,3,4-trihydroxybenz[a]anthracenyl)]-2'-deoxyguanosyl adduct in d(G(1)G(2)C(3)A(4)G(5)X(6)T(7)G(8)G(9)T(10)G(11)).d(C(12)A(13)C(14)C(15)A(16)C(17)C(18)T(19)G(20)C(21)C(22)), bearing codon 12 of the human N-ras protooncogene (underlined), was ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi020462k
更新日期:2003-03-04 00:00:00
abstract::The precise nature of redox modulation of N-methyl-d-aspartate (NMDA) receptors is still unclear, although it is thought to be related to the formation and breaking of disulfide bonds. Recent structural data demonstrated the way in which disulfide bonds in the ligand-binding core of the NR1 subunit are arranged. Howev...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061462d
更新日期:2007-02-27 00:00:00
abstract::We have found that the atomic force microscope (AFM) can be used to image the "beads-on-a-string" chromatin structure in a normal air environment following adsorption onto a cover glass substrate. Individual nucleosome cores and linker DNA could be resolved clearly along chromatin fibers that were reconstituted using ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00084a002
更新日期:1993-08-24 00:00:00
abstract::Phospholipids containing a saturated fatty acid in the primary position and an unsaturated fatty acid in the secondary position are a major structural part of biological membranes. The mixed-chain hydrophobic core of the membranes is the diacylglycerol part. To better understand the core properties of membranes we hav...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00051a015
更新日期:1995-12-26 00:00:00
abstract::tRNA-guanine transglycosylase (TGT) is a key enzyme involved in the post-transcriptional modification of certain tRNAs in their anticodon wobble positions with queuine. To maintain the correct Watson-Crick base pairing properties of the wobble base (and hence proper translation of the genetic code), TGT must recognize...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051863d
更新日期:2006-01-17 00:00:00
abstract::The enzymes in the catechol meta-fission pathway have been studied for more than 50 years in several species of bacteria capable of degrading a number of aromatic compounds. In a related pathway, naphthalene, a toxic polycyclic aromatic hydrocarbon, is fully degraded to intermediates of the tricarboxylic acid cycle by...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00050
更新日期:2016-05-10 00:00:00
abstract::Single-stranded microRNAs (miRNAs) regulate gene expression by triggering mRNA degradation and/or inhibiting mRNA translation. miRNAs play important roles in various critical cellular processes and are associated with numerous human diseases, including cancer and neurodegenerative diseases. miRNA sequences are embedde...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/acs.biochem.8b00944
更新日期:2019-01-29 00:00:00
abstract::The Haemophilus influenzae diaminopimelate epimerase was cloned, expressed, purified, and crystallized in the C2221 space group (a = 102.1 A, b = 115.4 A, c = 66.3 A, alpha = beta = gamma = 90 degrees). The three-dimensional structure was solved to 2.7 A using a single Pt derivative and the Se-Met-substituted enzyme t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi982138o
更新日期:1998-11-24 00:00:00
abstract::The calcium-dependent binding of two synthetic 18-residue peptides derived from the calmodulin binding region of skeletal myosin light chain kinase to wild-type Drosophila melanogaster calmodulin and four calcium binding site calmodulin mutants has been investigated using optical spectroscopy. The WFF peptide (with W4...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00007a001
更新日期:1995-02-21 00:00:00
abstract::In a previous paper, we reported the presence of globoside as a major neutral glycolipid in PC12 pheochromocytoma cells [Ariga, T., Macala, L. J., Saito, M., Margolis, R. K., Greene, L. A., Margolis, R. U., & Yu, R. K. (1988) Biochemistry 27, 52-58]. Recently, we found that subcloned PC12h cells accumulated another un...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00414a058
更新日期:1988-07-12 00:00:00
abstract::Three unfolding domains in rabbit muscle aldolase destabilized in 3 M urea have been identified from their unfolding rate constants (0.10, 0.036, and 0.0064 min-1). The populations of folded and various, partially unfolded forms were determined by amide hydrogen exchange and mass spectrometry. Results of this study sh...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972711o
更新日期:1998-05-05 00:00:00
abstract::We have covalently attached an electron paramagnetic resonance (EPR) spin probe to Cys-670 of the motor domain of ncd (nonclaret disjunctional protein) in order to investigate conformational changes associated with the chemomechanical cycle. Spin-labeling is highly specific and does not affect ncd function as monitore...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9706881
更新日期:1997-08-12 00:00:00
abstract::We have described earlier a chromatin-bound protease with unique specificity for histone H2A [Eickbush, T. H., Watson, D. K., & Moudrianakis, E. N. (1976) Cell (Cambridge, Mass.) 9, 785--792]. In the present study, we explore the nature of interactions that form and stabilize the enzyme-chromatin system by using the a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00531a008
更新日期:1982-01-19 00:00:00
abstract::The interaction of bleomycin A2 with DNA has been examined by fluorescence spectroscopy and proton magnetic resonance techniques. Fluorescence bands observed at 353 and 405 nm in the spectrum of bleomycin were assigned to the bithiazole and 4-aminopyrimidine rings, respectively. Quenching of bithiazole fluorescence by...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00635a021
更新日期:1977-08-09 00:00:00
abstract::The solvent polarity dependence of the interaction between thiamin and tryptophan was studied by spectrophotometric methods. The ultraviolet (UV) data clearly indicate that the interaction is weakened when the complex is transferred from water to aqueous ethanol or aqueous dioxane. The interaction of thiamin and trypt...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00625a012
更新日期:1977-03-22 00:00:00
abstract::The general view on Rhizobium chitolipooligosaccharides (CLOS) is that they are made in very low levels as diffusible molecules and are primarily secreted by the bacteria into the extracellular milieu where they interact with the host. However, the structural and predicted physicochemical properties of these amphiphil...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00011a041
更新日期:1995-03-21 00:00:00
abstract::Carbohydrate-binding module (CBM) family 13 includes the "R-type" or "ricin superfamily" beta-trefoil lectins. The C-terminal CBM, CBM13, of xylanase 10A from Streptomyces lividans is a family 13 CBM that is not only structurally similar to the "R-type" lectins but also somewhat functionally similar. The primary funct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi015865j
更新日期:2002-04-02 00:00:00
abstract::The natural product hypericin is a photosensitive polycyclic aromatic dione compound, which has been widely investigated because of its virucidal and antitumor properties. Although it has been suggested that singlet oxygen or a radical species might be responsible for its biological action, its mechanism of action rem...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi991147+
更新日期:1999-08-31 00:00:00
abstract::Rat organic anion transporting protein 1a1 (oatp1a1), a hepatocyte basolateral plasma membrane protein, mediates transport of various amphipathic compounds. Our previous studies indicated that serine phosphorylation of a single tryptic peptide inhibits its transport activity without changing its cell surface content. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052437v
更新日期:2006-03-14 00:00:00
abstract::The product of transesterification of phospholipid acyl chains and unesterified cholesterol (UC) by the enzyme lecithin:cholesterol acyltransferase (LCAT) is cholesteryl ester (CE). Activation of LCAT by apolipoprotein (apo) A-I on nascent (discoidal) high-density lipoproteins (HDL) is essential for formation of matur...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi901242k
更新日期:2009-12-01 00:00:00
abstract::Folding experiments have suggested that some proteins have kinetic intermediates with a non-native structure. A simple G ̅o model does not explain such non-native intermediates. Therefore, the folding energy landscape of proteins with non-native intermediates should have characteristic properties. To identify such pro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200241a
更新日期:2011-07-26 00:00:00
abstract::Pokeweed antiviral protein (PAP) is a single-chain ribosome inactivating protein (RIP) that binds to ribosomes and depurinates the highly conserved alpha-sarcin/ricin loop (SRL) of the large subunit rRNA. Catalytic depurination of a specific adenine has been proposed to result in translation arrest and cytotoxicity. H...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi048188c
更新日期:2005-02-22 00:00:00
abstract::The complete amino acid sequence of a small, basic protein with cardiotoxic activity is described. This toxin, designated Naja naja F8, was isolated from the venom of Naja naja, of Cambodian origin, by gel filtration on Sephadex G-75 followed by gradient ion exchange chromatography on Bio-Rex 70. The cardiotoxin F8, m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00684a011
更新日期:1975-07-01 00:00:00
abstract::Previous optical and electron paramagnetic resonance (EPR) spectroscopic studies of the newly discovered peroxynitrite scavenging pseudoperoxidase from Leishmania major (LmPP) suggested that ferric LmPP contained a six-coordinate low-spin (6cLS) heme with a thiolate ligand, presumably a cysteine, bound to its heme iro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi401343t
更新日期:2013-12-10 00:00:00
abstract::The interaction of solvent of the substrate binding domain of the bacterial heat shock 70 chaperone protein DnaK was studied in its apo form and with bound hydrophobic substrate peptide, using refined nuclear magnetic resonance experiments. Distinct differences between the two states of the protein were observed. Acco...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi030097c
更新日期:2003-09-30 00:00:00
abstract::The constant (CL) fragment of the immunoglobulin light chain contains only one intrachain disulfide bond buried in the interior of the molecule. The kinetics of reduction with dithiothreitol of the disulfide bond were studied at various concentrations of guanidine hydrochloride at pH 8.0 and 25 degrees C. It was found...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00356a026
更新日期:1986-04-22 00:00:00
abstract::alpha-Glucosidase (EC 3.2.1.20) was purified to homogeneity from logarithmically growing cells of Saccharomyces carlsbergensis. The purification involved the following steps: (a) ammonium sulfate fractionation; (b) Sephadex G-100 chromatography; (c) DEAE-cellulose chromatography; and (d) hydroxylapatite chromatography...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00615a011
更新日期:1978-10-31 00:00:00
abstract::The epidermal growth factor receptor is a transmembrane glycoprotein that mediates the cellular responses to epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha). In this study of the human EGF receptor naturally expressed in A431 cells, the glycosylation sites of the full-length, membrane-bo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi027101p
更新日期:2003-05-13 00:00:00