Characterization of glycosylation sites of the epidermal growth factor receptor.


:The epidermal growth factor receptor is a transmembrane glycoprotein that mediates the cellular responses to epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha). In this study of the human EGF receptor naturally expressed in A431 cells, the glycosylation sites of the full-length, membrane-bound receptor and of a secreted form of the receptor were characterized by mass spectrometry. Our data show that the naturally expressed human EGF receptor is fully glycosylated on eight of the 11 canonical sites; two of the sites are not glycosylated, and one is partially glycosylated, a pattern of site-usage similar but not identical to those reported for the recombinant human EGF receptor heterologously expressed in Chinese hamster ovary cells. We also confirm the partial glycosylation of an atypical NNC site first identified in the receptor expressed in Chinese hamster ovary cells. We show that an additional canonical site in the secreted form of the receptor is fully glycosylated. While the pattern of glycosylation is the same for the sites shared by the full-length and the secreted forms of the receptor, the oligosaccharides of the full-length receptor are more extensively processed. Finally, we provide evidence that in addition to the known secreted form of the receptor, a proteolytic cleavage product of the receptor corresponding to the full extracytoplasmic, ligand-binding domain is present in the conditioned medium.






Zhen Y,Caprioli RM,Staros JV




Has Abstract


2003-05-13 00:00:00












  • Time-resolved absorption and emission show that the CP43' antenna ring of iron-stressed synechocystis sp. PCC6803 is efficiently coupled to the photosystem I reaction center core.

    abstract::Excitation energy transfer and trapping processes in an iron stress-induced supercomplex of photosystem I from the cyanobacterium Synechocystis sp. PCC6803 were studied by time-resolved absorption and fluorescence spectroscopy on femtosecond and picosecond time scales. The data provide evidence that the energy transfe...


    pub_type: 杂志文章


    authors: Melkozernov AN,Bibby TS,Lin S,Barber J,Blankenship RE

    更新日期:2003-04-08 00:00:00

  • Lipid Membranes Influence the Ability of Small Molecules To Inhibit Huntingtin Fibrillization.

    abstract::Several diseases, including Alzheimer's disease, Parkinson's disease, and Huntington's disease (HD), are associated with specific proteins aggregating and depositing within tissues and/or cellular compartments. The aggregation of these proteins is characterized by the formation of extended, β-sheet rich fibrils, terme...


    pub_type: 杂志文章


    authors: Beasley M,Stonebraker AR,Hasan I,Kapp KL,Liang BJ,Agarwal G,Groover S,Sedighi F,Legleiter J

    更新日期:2019-10-29 00:00:00

  • Rational modification of protein stability by the mutation of charged surface residues.

    abstract::Continuum methods were used to calculate the electrostatic contributions of charged and polar side chains to the overall stability of a small 41-residue helical protein, the peripheral subunit-binding domain. The results of these calculations suggest several residues that are destabilizing, relative to hydrophobic iso...


    pub_type: 杂志文章


    authors: Spector S,Wang M,Carp SA,Robblee J,Hendsch ZS,Fairman R,Tidor B,Raleigh DP

    更新日期:2000-02-08 00:00:00

  • Cloning of synthetic deoxyribonucleic acid that codes for embryonic cardiac myosin light-chain polypeptide.

    abstract::Double-stranded complementary deoxyribonucleic acid (cDNA) transcribed in vitro from a partially pure myosin light-chain messenger ribonucleic acid (mRNA) of the chick embryonic heart was cloned in Escherichia coli strain chi 1776 by using the HindIII cleavage site in the plasmid pBR322. The insertion of essentially f...


    pub_type: 杂志文章


    authors: Arnold HH,Siddiqui MA

    更新日期:1979-12-11 00:00:00

  • Hydrogen exchange kinetics changes upon formation of the soybean trypsin inhibitor-trypsin complex.

    abstract::The hydrogen exchange kinetics of the complex of trypsin-soybean trypsin inhibitor (Kunitz) have been compared to the calculated sum of the exchange kinetics for the inhibitor and trypsin measured separately. The exchange rates observed for the complex are substantially less than the sum of the exchange rates in the t...


    pub_type: 杂志文章


    authors: Woodward CK,Ellis LM

    更新日期:1975-07-29 00:00:00

  • Defining the precision with which a protein structure is determined by NMR. Application to motilin.

    abstract::A simple procedure is introduced for accurately defining the precision with which the Cartesian coordinates of any macromolecular structure are determined by nuclear Overhauser data. The method utilizes an ensemble of structures obtained from an array of independent simulated data sets derived from a final structure. ...


    pub_type: 杂志文章


    authors: Shriver J,Edmondson S

    更新日期:1993-02-16 00:00:00

  • Partial denaturation of transthyretin is sufficient for amyloid fibril formation in vitro.

    abstract::Amyloid diseases are caused by the self-assembly of a given protein into an insoluble cross-beta-sheet quaternary structural form which is pathogenic. An understanding of the biochemical mechanism of amyloid fibril formation should prove useful in understanding amyloid disease. Toward this end, a procedure for the con...


    pub_type: 杂志文章


    authors: Colon W,Kelly JW

    更新日期:1992-09-15 00:00:00

  • Cdx1 interacts physically with a subset of Hox proteins.

    abstract::Cdx and Hox gene families encode homeodomain-containing transcription factors involved in anterior-posterior vertebral patterning. Although Cdx proteins are direct transcriptional regulators of Hox gene expression, both Hox and Cdx proteins are known to interact with other homeodomain transcription factors, leading us...


    pub_type: 杂志文章


    authors: Lafontaine CA,Grainger S,Hess BL,Béland M,Lohnes D

    更新日期:2012-12-04 00:00:00

  • Photoconversion of 7-dehydrocholesterol to vitamin D3 in synthetic phospholipid bilayers.

    abstract::The incorporation of 7-dehydrocholesterol into synthetic phospholipid bilayers altered the distribution of products after photolysis. In liposomes, the relative amounts of 7-dehydrocholesterol and lumisterol were elevated, and tachysterol was reduced from the levels observed in hexane solution. Z to E isomerization of...


    pub_type: 杂志文章


    authors: Yamamoto JK,Borch RF

    更新日期:1985-06-18 00:00:00

  • Aggregation of Alzheimer's amyloid β-peptide in biological membranes: a molecular dynamics study.

    abstract::Numerous studies have concluded that the interaction of the amyloid β-peptide (Aβ) and cellular membranes contributes to the toxicity and cell death observed in the progression of Alzheimer's disease. Aggregated Aβ species disrupt membranes, leading to physical instability and ion leakage. Further, the presence of Aβ ...


    pub_type: 杂志文章


    authors: Lemkul JA,Bevan DR

    更新日期:2013-07-23 00:00:00

  • Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 2. Unusual photophysical properties.

    abstract::In the preceding accompanying paper [Shu, X., et al. (2007) Biochemistry 46, 12005-12013], the 1.5 A resolution crystal structure of green fluorescent protein (GFP) variant S65T/H148D is presented, and the possible consequences of an unusual short hydrogen bond (


    pub_type: 杂志文章


    authors: Shi X,Abbyad P,Shu X,Kallio K,Kanchanawong P,Childs W,Remington SJ,Boxer SG

    更新日期:2007-10-30 00:00:00

  • Effects of hydration on purine motion in solid DNA.

    abstract::Deuterium quadrupole echo spectra and spin-lattice relaxation rates measured at 76.8 and 38.4 MHz as a function of relative humidity are reported for calf thymus DNA deuterated at positions A8 and G8. The amplitude of base pair motion is observed to increase slightly with increasing degree of hydration (up to approxim...


    pub_type: 杂志文章


    authors: Brandes R,Vold RR,Vold RL,Kearns DR

    更新日期:1986-11-18 00:00:00

  • Biochemical characterization of the HpxO enzyme from Klebsiella pneumoniae, a novel FAD-dependent urate oxidase.

    abstract::The HpxO enzyme from Klebsiella pneumoniae was recently proposed, on the basis of genetic studies, to catalyze the hydroxylation of uric acid to 5-hydroxyisourate as part of the purine catabolic pathway. Its primary sequence suggests that the HpxO catalytic activity depends on a flavin cofactor (FAD), contrasting with...


    pub_type: 杂志文章


    authors: O'Leary SE,Hicks KA,Ealick SE,Begley TP

    更新日期:2009-04-14 00:00:00

  • Chemical modification of mitochondrial transhydrogenase: evidence for two classes of sulfhydryl groups.

    abstract::Chemical-modification studies on submitochondrial particle pyridine dinucleotide transhydrogenase (EC demonstrate the presence of one class of sulfhydryl group in the nicotinamide adenine dinucleotide phosphate (NADP) site and another peripheral to the active site. Reaction of the peripheral sulfhydryl group ...


    pub_type: 杂志文章


    authors: Earle SR,O'Neal SG,Fisher RR

    更新日期:1978-10-31 00:00:00

  • Oxidation-reduction cycles of peroxiredoxin proteins and nontranscriptional aspects of timekeeping.

    abstract::The circadian clock allows organisms to accurately predict the earth's rotation and modify their behavior as a result. Genetic analyses in a variety of organisms have defined a mechanism based largely on gene expression feedback loops. However, as we delve more deeply into the mechanisms of circadian timekeeping, we a...


    pub_type: 杂志文章,评审


    authors: Hoyle NP,O'Neill JS

    更新日期:2015-01-20 00:00:00

  • Mutation at active site lysine 212 to arginine uncouples the glycosylase activity from the lyase activity of human endonuclease III.

    abstract::The human endonuclease III (hNTH1) is an important DNA glycosylase with associated abasic lyase activity. We previously demonstrated that the K212Q mutant was totally inactive, while the K212R mutant had reduced DNA glycosylase/lyase activity and could form a covalent complex with the substrate DNA upon reduction. We ...


    pub_type: 杂志文章


    authors: Liu X,Roy R

    更新日期:2001-11-13 00:00:00

  • The hemophore HasA from Yersinia pestis (HasAyp) coordinates hemin with a single residue, Tyr75, and with minimal conformational change.

    abstract::Hemophores from Serratia marcescens (HasA(sm)) and Pseudomonas aeruginosa (HasA(p)) bind hemin between two loops, which harbor the axial ligands H32 and Y75. Hemin binding to the Y75 loop triggers closing of the H32 loop and enables binding of H32. Because Yersinia pestis HasA (HasA(yp)) presents a Gln at position 32,...


    pub_type: 杂志文章


    authors: Kumar R,Lovell S,Matsumura H,Battaile KP,Moënne-Loccoz P,Rivera M

    更新日期:2013-04-23 00:00:00

  • Local-access model for proton transfer in bacteriorhodopsin.

    abstract::The accessibility of the retinal Schiff base in bacteriorhodopsin was studied in the D85N/D96N mutant where the proton acceptor and donor are absent. Protonation and deprotonation of the Schiff base after pH jump without illumination and in the photocycle of the unprotonated Schiff base were measured in the visible an...


    pub_type: 杂志文章


    authors: Brown LS,Dioumaev AK,Needleman R,Lanyi JK

    更新日期:1998-03-17 00:00:00

  • Ia-associated invariant chain is fatty acylated before addition of sialic acid.

    abstract::The murine invariant chain (Ii) was found to incorporate radioactive palmitic acid. This binding of fatty acid inhibits the formation of interchain S-S bonds, probably because the cysteine residue in the transmembrane region of the Ii chain is palmitylated. The inhibition of fatty acylation by cerulenin blocks further...


    pub_type: 杂志文章


    authors: Koch N,Hämmerling GJ

    更新日期:1985-10-22 00:00:00

  • HO. and DNase I probing of E sigma 70 RNA polymerase--lambda PR promoter open complexes: Mg2+ binding and its structural consequences at the transcription start site.

    abstract::Chemical and enzymatic probing (footprinting) of the reactivity of the promoter DNA backbone is applied to characterize two binary open complexes RPo1 (-Mg2+) and RPo2 (+Mg2+), formed by Escherichia coli RNA polymerase (E sigma 70) at the lambda PR promoter. We report that HO. detects major differences in backbone rea...


    pub_type: 杂志文章


    authors: Craig ML,Suh WC,Record MT Jr

    更新日期:1995-12-05 00:00:00

  • A "Seleno Effect" Differentiates the Roles of Redox Active Cysteine Residues in Plasmodium falciparum Thioredoxin Reductase.

    abstract::Here, we introduce the concept of the "seleno effect" in the study of oxidoreductases that catalyze thiol/disulfide exchange reactions. In these reactions, selenium can replace sulfur as a nucleophile, electrophile, or leaving group, and the resulting change in rate (the seleno effect) is defined as kS/ kSe. In soluti...


    pub_type: 杂志文章


    authors: O'Keefe JP,Dustin CM,Barber D,Snider GW,Hondal RJ

    更新日期:2018-03-20 00:00:00

  • Multiple metal binding domains enhance the Zn(II) selectivity of the divalent metal ion transporter AztA.

    abstract::Transition metal-transporting P1B-type CPx ATPases play crucial roles in mediating metal homeostasis and resistance in all cells. The degree to which N-terminal metal binding domains (MBDs) confer metal specificity to the transporter is unclear. We show that the two MBDs of the Zn/Cd/Pb effluxing pump Anabaena AztA ar...


    pub_type: 杂志文章


    authors: Liu T,Reyes-Caballero H,Li C,Scott RA,Giedroc DP

    更新日期:2007-10-02 00:00:00

  • Analysis of a distal cluster of binding elements and other unusual features of the promoter of the highly regulated nit-3 gene of Neurospora crassa.

    abstract::In Neurospora crassa, the expression of the nit-3 gene (nitrate reductase) is dependent upon nitrogen derepression and nitrate induction and is regulated by two positive-acting transcription factors, NIT2 and NIT4, and a negative regulator, NMR. The presence of a tightly linked cluster of NIT2 and NIT4 binding sites s...


    pub_type: 杂志文章


    authors: Tao Y,Marzluf GA

    更新日期:1998-08-04 00:00:00

  • Chemical modification of alpha-subunit tryptophan residues in Schizosaccharomyces pombe mitochondrial F1 adenosine 5'-triphosphatase: differential reactivity and role in activity.

    abstract::Chemical modification of mitochondrial F1-ATPase from Schizosaccharomyces pombe by the tryptophan-specific reagent N-bromosuccinimide (NBS) at pH 5.0 in the presence of 20% glycerol produced a characteristic lowering in both enzyme absorbance at 280 nm and intrinsic fluorescence at 332 nm that varied with NBS/F1 molar...


    pub_type: 杂志文章


    authors: Divita G,Jault JM,Gautheron DC,Di Pietro A

    更新日期:1993-02-02 00:00:00

  • Structural Elucidation of Peptide Binding to KLHL-12, a Substrate Specific Adapter Protein in a Cul3-Ring E3 Ligase Complex.

    abstract::KLHL-12 is a substrate specific adapter protein for a Cul3-Ring ligase complex. It is a member of the Kelch β-propeller domain subclass of Cullin-Ring substrate recognition domains. This E3 ubiquitin ligase complex has many activities, including acting as a negative regulator of the Wnt signaling pathway by mediating ...


    pub_type: 杂志文章


    authors: Zhao B,Payne WG,Sai J,Lu Z,Olejniczak ET,Fesik SW

    更新日期:2020-03-03 00:00:00

  • Catalytic roles of the distal site asparagine-histidine couple in peroxidases.

    abstract::There are highly conserved hydrogen bonds between the distal His and the adjacent Asn in many peroxidases. Although the crystal structure of horseradish peroxidase C (HRP) is not available, comparison of the amino acid sequence with cytochrome c peroxidase indicates that Asn70 is making the hydrogen bond with the dist...


    pub_type: 杂志文章


    authors: Nagano S,Tanaka M,Ishimori K,Watanabe Y,Morishima I

    更新日期:1996-11-12 00:00:00

  • The amino acid sequence of chick skin collagen alpha1-CB7.

    abstract::The amino acid sequence of chick skin collagen alpha1-CB7, the 268 CNBr peptide from the helical portion of the alpha1 chain, has been determined by automatic and manual degradation of tryptic and chymotryptic peptides, and of the COOH-terminal fragment produced by cleavage with animal collagenase. The resulting seque...


    pub_type: 杂志文章


    authors: Highberger JH,Corbett C,Kang AH,Gross J

    更新日期:1975-07-01 00:00:00

  • Redox modulation of endothelial nitric oxide synthase by glutaredoxin-1 through reversible oxidative post-translational modification.

    abstract::S-Glutathionylation is a redox-regulated modification that uncouples endothelial nitric oxide synthase (eNOS), switching its function from nitric oxide (NO) synthesis to (•)O2(-) generation, and serves to regulate vascular function. While in vitro or in vivo eNOS S-glutathionylation with modification of Cys689 and Cys...


    pub_type: 杂志文章


    authors: Chen CA,De Pascali F,Basye A,Hemann C,Zweier JL

    更新日期:2013-09-24 00:00:00

  • Comparison of NMR solution structures of the receptor binding domains of Pseudomonas aeruginosa pili strains PAO, KB7, and PAK: implications for receptor binding and synthetic vaccine design.

    abstract::The solution structures of peptide antigens from the receptor binding domains of Pseudomonas aeruginosa strains PAO and KB7 have been determined using two-dimensional 1H NMR techniques. Ensembles of solution conformations for the trans forms of these 17-residue disulfide-bridged peptides have been generated using a si...


    pub_type: 杂志文章


    authors: Campbell AP,McInnes C,Hodges RS,Sykes BD

    更新日期:1995-12-19 00:00:00

  • Partitioning of fish and insect antifreeze proteins into ice suggests they bind with comparable affinity.

    abstract::Antifreeze proteins (AFPs) inhibit the growth of ice by binding to the surface of ice crystals, preventing the addition of water molecules to cause a local depression of the freezing point. AFPs from insects are much more effective at depressing the freezing point than fish AFPs. Here, we have investigated the possibi...


    pub_type: 杂志文章


    authors: Marshall CB,Tomczak MM,Gauthier SY,Kuiper MJ,Lankin C,Walker VK,Davies PL

    更新日期:2004-01-13 00:00:00