Abstract:
:Tetraheme cytochromes involved in photosynthetic electron transport have previously been described associated with the reaction centers of purple photosynthetic bacteria; however, similar heme proteins have not until now been characterized in the phylogenetically distinct green sulfur bacteria. In this paper we describe the first isolation and characterization of a multitheme, membrane-associated cytochrome from a green sulfur bacterium, Chlorobium limicola forma thiosulfatophilum. We show that this cytochrome contains a single polypeptide of 32 kDa apparent molecular mass on SDS-PAGE and has a characteristic broad alpha-band absorption at 553 nm. By both low-temperature absorption and electron paramagnetic resonance spectroscopy, we demonstrate that there are at least four distinct heme groups.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Albouy D,Sturgis JN,Feiler U,Nitschke W,Robert Bdoi
10.1021/bi962624gsubject
Has Abstractpub_date
1997-02-18 00:00:00pages
1927-32issue
7eissn
0006-2960issn
1520-4995pii
bi962624gjournal_volume
36pub_type
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