Abstract：
:Catalase HPII from Escherichia coli is a homotetramer of 753 residue subunits. The multimer displays a number of unusual structural features, including interwoven subunits and a covalent bond between Tyr415 and His392, that would contribute to its rigidity and stability. As the temperature of a solution of HPII in 50 mM potassium phosphate buffer (pH 7) is raised from 50 to 92 degrees C, the enzyme begins to lose activity at 78 degrees C and 50% inactivation has occurred at 83 degrees C. The inactivation is accompanied by absorbance changes at 280 and 407 nm and by changes in the CD spectrum consistent with small changes in secondary structure. The subunits in the dimer structure remain associated at 95 degrees C and show a significant level of dissociation only at 100 degrees C. The exceptional stability of the dimer association is consistent with the interwoven nature of the subunits and provides an explanation for the resistance to inactivation of the enzyme. For comparison, catalase-peroxidase HPI of E. coli and bovine liver catalase are 50% inactivated at 53 and 56 degrees C, respectively. In 5.6 M urea, HPII exhibits a coincidence of inactivation, CD spectral change, and dissociation of the dimer structure with a midpoint of 65 degrees C. The inactive mutant variants of HPII which fold poorly during synthesis and which lack the Tyr-His covalent bond undergo spectral changes in the 78 to 84 degrees C range, revealing that the extra covalent linkage is not important in the enhanced resistance to denaturation and that problems in the folding pathway do not affect the ultimate stability of the folded structure.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Switala J,O'Neil JO,Loewen PCdoi
10.1021/bi982863zsubject
Has Abstractpub_date
1999-03-30 00:00:00pages
3895-901issue
13eissn
0006-2960issn
1520-4995pii
bi982863zjournal_volume
38pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The Salmonella typhimurium PhoN protein is a nonspecific acid phosphatase and belongs to the phosphatidic acid phosphatase type 2 (PAP2) superfamily. We report here the crystal structures of phosphate-bound PhoN, the PhoN-tungstate complex, and the T159D mutant of PhoN along with functional characterization of three m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi062180g
更新日期：2007-02-27 00:00:00
abstract：:Receptor tyrosine kinase (RTK) activation is associated with modulation of heptahelical receptor-stimulated adenylyl cyclase responses. The mechanisms underlying the RTK-mediated enhancement of adenylyl cyclase function remain unclear. In the present studies, we show that the tyrosine kinase-dependent enhancement of a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0015818
更新日期：2001-02-13 00:00:00
abstract：:The membrane stabilization effect of cholesteryl hemisuccinate (CHEMS) and the sensitivity of the CHEMS-phosphatidylethanolamine membranes to protons and calcium ions were studied by differential scanning calorimetry, freeze-fracture electron microscopy, and 31P NMR. (1) At neutral pH, the addition of 8 mol % CHEMS to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00328a013
更新日期：1985-03-26 00:00:00
abstract：:Amyloid beta peptide (A beta) is released into the media of a variety of cells in culture during normal metabolism. The discovery of several missense mutations within or flanking the A beta region of the beta amyloid precursor protein (beta APP) in familial Alzheimer's disease provides strong evidence for a role of al...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00181a016
更新日期：1994-04-19 00:00:00
abstract：:Restrictocin and related fungal endoribonucleases from the α-sarcin family site-specifically cleave the sarcin/ricin loop (SRL) on the ribosome to inhibit translation and ultimately trigger cell death. Previous studies showed that the SRL folds into a bulged-G motif and tetraloop, with restrictocin achieving a specifi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1018336
更新日期：2011-04-12 00:00:00
abstract：:Deamidation of proteins is one of the most prevalent post-translational modifications found upon aging, and in age-onset diseases. Specific asparagine and glutamine residues are often selectively deamidated during this process. In the human lens, deamidation has been shown to occur in many crystallins, but it is not c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00185
更新日期：2015-08-11 00:00:00
abstract：:The P22 tailspike endorhamnosidase confers the high specificity of bacteriophage P22 for some serogroups of Salmonella differing only slightly in their O-antigen polysaccharide. We used several biophysical methods to study the binding and hydrolysis of O-antigen fragments of different lengths by P22 tailspike protein....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.0c00872
更新日期：2020-12-29 00:00:00
abstract：:Cytochrome oxidase proteoliposomes were prepared from bovine heart oxidase. Size distributions determined by quasi-elastic light scattering (QELS) showed that there was a small population of large vesicles (120-200-nm diameter) and a large population of small vesicles (50-100-nm diameter). Trapping cytochrome c inside...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00468a010
更新日期：1990-04-24 00:00:00
abstract：:EL222 is a blue light sensor protein, which consists of a light-oxygen-voltage domain as a light sensor and a LuxR-type helix-turn-helix DNA-binding domain. The reaction dynamics of the protein-DNA binding were observed for the first time using the time-resolved transient grating method. The reaction scheme was determ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b01206
更新日期：2018-03-13 00:00:00
abstract：:Thioltransferase activity was identified and the enzyme purified to apparent homogeneity from human red blood cells. Activity was measured as glutathione-dependent reduction of the prototype substrate hydroxyethyl disulfide; formation of oxidized glutathione (GSSG) was coupled to NADPH oxidation by GSSG reductase (1 u...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00239a002
更新日期：1991-06-25 00:00:00
abstract：:DNA damage may alter the outcome of protein-nucleic acid interactions. The malondialdehyde-deoxyguanosine adduct, 3-(2'-deoxy-beta-d-erythro-pentofuranosyl)pyrimido[1,2-alpha]purin-10-(3H)-one (M(1)dG), miscodes in vivo and in vitro. M(1)dG is an exocyclic adduct that undergoes ring-opening in duplex DNA to form the a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0472898
更新日期：2005-04-05 00:00:00
abstract：:In this work, we show that adenosine 5'-O-(3-thiotriphosphate) (ATP gamma S) is a substrate for yeast inorganic pyrophosphatase (PPase) (EC 3.6.1.1) and further, using chirally labeled [gamma-17O,18O]ATP gamma S, that enzyme-catalyzed hydrolysis to produce chiral inorganic thio[17O,18O]phosphate proceeds with inversio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00300a002
更新日期：1984-02-28 00:00:00
abstract：:The Hsp70-class molecular chaperone HscA interacts specifically with a conserved (99)LPPVK(103) motif of the iron-sulfur cluster scaffold protein IscU. We used a cellulose-bound peptide array to perform single-site saturation substitution of peptide residues corresponding to Glu(98)-Ile(104) of IscU to determine posit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0606187
更新日期：2006-07-04 00:00:00
abstract：:RP2 is a ubiquitously expressed protein encoded by a gene associated with X-linked retinitis pigmentosa (XLRP), a retinal degenerative disease that causes severe vision loss. Previous in vitro studies have shown that RP2 binds to ADP ribosylation factor-like 3 (Arl3) and activates its intrinsic GTPase activity, but th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1005249
更新日期：2010-09-07 00:00:00
abstract：:In mammalian cells, the incorporation of the 21st amino acid, selenocysteine, into proteins is guided by the Sec machinery. The function of this protein complex requires several protein-protein and protein-RNA interactions, leading to the incorporation of selenocysteine at UGA codons. It is guided by stem-loop structu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b01116
更新日期：2017-05-02 00:00:00
abstract：:Synthetic O-glycopeptides containing one or two GalNAc residues attached to Ser or Thr were used as substrates to investigate the effect of peptide structure on the activity of crude preparations of UDP-Gal:GalNAc alpha-R beta 3-Gal-transferase from pig stomach and pig and rat colonic mucosa and of a partially purifie...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00496a008
更新日期：1990-11-06 00:00:00
abstract：:A T203Y substitution in green fluorescent protein causes a red shift in emission to yield a class of mutants known as yellow fluorescent protein (YFP). Many of these YFP mutants bind halides with affinities in the millimolar range, which often results in the chromophore pK values being shifted into the physiological r...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi3016839
更新日期：2013-04-09 00:00:00
abstract：:The proximal bond between the iron atom of the heme group and the N epsilon of histidine F8 in myoglobin (Mb) and hemoglobin (Hb) is presumed to be an important determinant of heme binding, protein structure, and oxygen binding. Here a system is described in which the proximal ligand is provided intermolecularly by th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00187a023
更新日期：1994-05-31 00:00:00
abstract：:The fidelity of the poliovirus RNA-dependent RNA polymerase (3D(pol)) plays a direct role in the genomic evolution and pathogenesis of the virus. A single site mutation (Gly64Ser) that is remote from the catalytic center results in a higher fidelity polymerase. NMR studies with [methyl-(13)C]methionine-labeled protein...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100833r
更新日期：2010-11-02 00:00:00
abstract：:Lipoprotein(a) [Lp(a)] is a unique lipoprotein consisting of a low-density lipoprotein moiety (LDL) covalently linked to apoprotein(a). Previous work has demonstrated that Lp(a) can compete with plasminogen (PGN) for binding to endothelial and mononuclear cells and can inhibit PGN activation in cell-free systems. We h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00068a021
更新日期：1993-05-04 00:00:00
abstract：:Acetohydroxyacid synthase (AHAS, EC 4.1.3.18) catalyzes the first step in the biosynthesis of the branched-chain amino acids. In bacteria, the enzyme has a large subunit containing the catalytic machinery and a small subunit with a regulatory role. In eucaryotes, the evidence for a regulatory subunit is largely indire...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi983013m
更新日期：1999-04-20 00:00:00
abstract：:The nonbulky 5',8-cyclopurine DNA lesions (cP) and the bulky, benzo[ a]pyrene diol epoxide-derived stereoisomeric cis- and trans- N2-guanine adducts (BPDE-dG) are good substrates of the human nucleotide excision repair (NER) mechanism. These DNA lesions were embedded at the In or Out rotational settings near the dyad ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b01066
更新日期：2019-02-12 00:00:00
abstract：:Phospholipase A2 from Naja naja naja venom (Indian cobra) undergoes a concentration-dependent aggregation, and at an assay concentration of 1 microgram mL-1, it exists as a monomer. However, there is some evidence that the enzyme is actually active as a dimer or higher order aggregate. Previous attempts to determine t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00343a018
更新日期：1985-10-22 00:00:00
abstract：:The three-dimensional solution structure of toxin FS2, a 60-residue polypeptide isolated from the venom of black mamba snake (Dendroaspis polylepis polylepis), has been determined by nuclear magnetic resonance spectroscopy. Using 600 NOE constraints and 55 dihedral angle constraints, a set of 20 structures obtained fr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00017a022
更新日期：1995-05-02 00:00:00
abstract：:The topography of the photosynthetic reaction center (RC) polypeptides (H, M, and L) was investigated by proteolysis and radioiodination of membrane vesicles isolated from Rhodopseudomonas sphaeroides. Chromatophores, obtained from French-pressed cell lysates, are closed vesicles' and oriented inside out with respect ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00519a012
更新日期：1981-08-04 00:00:00
abstract：:Electronic absorption and resonance Raman spectra, together with rates of arsenolysis, are used to show that the acylated subunits of the rabbit furylacryloylglyceraldehyde-3-phosphate dehydrogenase (FA-rabbit enzyme), but not the FA-sturgeon enzyme, exist as a mixed population of at least two forms. One form of the F...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00515a023
更新日期：1981-06-09 00:00:00
abstract：:Preprotein translocation in Escherichia coli is mediated by the translocase with SecA as peripheral ATPase and SecY, SecE, and SecG as membrane domain. To facilitate large-scale purification of the SecYEG heterotrimer, SecY was fused at its amino terminus with a hexahistidine tag and co-overexpressed with SecE and Sec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972105t
更新日期：1998-01-06 00:00:00
abstract：:Volumetric characteristics of protein recognition events determine the direction of pressure-induced shifts in the recognition reaction, while also providing insights into the structural, dynamic, and hydration changes. We report changes in volume, ΔV, and adiabatic compressibility, ΔK(S), accompanying the binding of ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi3006994
更新日期：2012-07-24 00:00:00
abstract：:Resonance Raman (RR) spectra of soluble guanylate cyclase (sGC) reported by five independent research groups have been classified as two types: sGC(1) and sGC(2). Here we demonstrate that the RR spectra of sGC isolated from bovine lung contain only sGC(2) while both species are observed in the spectra of the CO-bound ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0489208
更新日期：2005-01-25 00:00:00
abstract：:Escherichia coli (A)BC excinuclease is the major enzyme responsible for removing bulky adducts, such as pyrimidine dimers and 6-4 photoproducts, from DNA. Mutants deficient in this enzyme are extremely sensitive to UV and UV-mimetic agents, but not to oxidizing agents, or ionizing radiation which damages DNA in part b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00446a002
更新日期：1989-10-03 00:00:00
