Accumulation of a globo-series glycolipid having Gal alpha 1-3Gal in PC12h pheochromocytoma cells.

abstract：:Protein-lipid interactions regulate many membrane protein functions. Using a multiscale approach that combines coarse-grained and atomistic molecular dynamics simulations, we have predicted the binding site for the anionic phospholipid phosphatidylinositol 4,5-bisphosphate (PIP(2)) on the Kir2.2 inwardly rectifying (K...

journal_title：Biochemistry

authors： Schmidt MR,Stansfeld PJ,Tucker SJ,Sansom MS

更新日期：2013-01-15 00:00:00

abstract：:The luminescence of Cu-substituted cytochrome c exhibits temperature-dependent decay modes and spectral shifts at temperatures below 80 K. By comparison with the data from metal-substituted porphyrins, we determined intramolecular rate parameters. The observed tripdoublet decay time is 13 +/- 1 mus at 77 K and the qua...

journal_title：Biochemistry

authors： Glatz P,Chance B,Vanderkooi JM

更新日期：1979-08-07 00:00:00

abstract：:Mammalian ribonucleotide reductase consists of two nonidentical subunits, proteins R1 and R2, each inactive alone. The R1 protein binds the ribonucleotide substrates while the R2 protein contains a binuclear iron center and a tyrosyl free radical, essential for activity. The crystal structures of the corresponding Esc...

journal_title：Biochemistry

authors： Rova U,Goodtzova K,Ingemarson R,Behravan G,Gräslund A,Thelander L

更新日期：1995-04-04 00:00:00

abstract：:Sulfenic acid is formed upon oxidation of thiols and is a central intermediate in the redox modulation of an increasing number of proteins. Methods for quantifying or even detecting sulfenic acid are scarce. Herein, the reagent 7-chloro-4-nitrobenz-2-oxa-1,3-diazole was determined not to be suitable as a chromophoric ...

journal_title：Biochemistry

authors： Turell L,Botti H,Carballal S,Ferrer-Sueta G,Souza JM,Durán R,Freeman BA,Radi R,Alvarez B

更新日期：2008-01-08 00:00:00

abstract：:The positional specificity is the decisive enzyme property for classification of lipoxygenases and for the currently used lipoxygenase nomenclature. It has been reported before that soybean lipoxygenase-1, which oxygenates polyenoic fatty acids at alkaline pH to the corresponding n - 6 hydroperoxy derivative, exhibits...

journal_title：Biochemistry

authors： Ivanov I,Rathmann J,Myagkova G,Kuhn H

更新日期：2001-08-28 00:00:00

abstract：:Inhibition of gamma-aminobutyric acid aminotransferase (GABA-AT) increases the concentration of GABA, an inhibitory neurotransmitter in human brain, which could have therapeutic applications for a variety of neurological diseases, including epilepsy. On the basis of studies of several previously synthesized conformati...

journal_title：Biochemistry

authors： Wang Z,Yuan H,Nikolic D,Van Breemen RB,Silverman RB

更新日期：2006-12-05 00:00:00

abstract：:The recA locus of pathogenic mycobacteria differs from that of nonpathogenic species because it contains large intervening sequences nested in the RecA homology region that are excised by an unusual protein-splicing reaction. In vivo assays indicated that Mycobacterium tuberculosis recA partially complemented Escheric...

journal_title：Biochemistry

authors： Kumar RA,Vaze MB,Chandra NR,Vijayan M,Muniyappa K

更新日期：1996-02-13 00:00:00

abstract：:The formation of pseudouridine (psi) on U5 small nuclear RNA (U5 snRNA) was studied using an in vitro modification system. Labeled U5 RNA, synthesized in vitro and therefore unmodified, was incubated in reactions containing S100 and/or nuclear extracts (NE) from HeLa cells, and the levels of psi were determined. There...

journal_title：Biochemistry

authors： Patton JR

更新日期：1994-08-30 00:00:00

abstract：:The toxin RelE is a ribosome-dependent endoribonuclease implicated in diverse cellular processes, including persistence. During amino acid starvation, RelE inhibits translation by cleaving ribosomal A-site mRNA. Although RelE is structurally similar to other microbial endoribonucleases, the active-site amino acid comp...

journal_title：Biochemistry

authors： Griffin MA,Davis JH,Strobel SA

更新日期：2013-12-03 00:00:00

abstract：:The dissociation of the trypsin-sensitive basic tails of the core histones in core chromatin has been followed as a function of [NaCl] using proton NMR spectroscopy. The tails dissociate in a highly cooperative all or none manner over the salt concentration range 0.2-0.6 M, that is, below the salt concentration requir...

journal_title：Biochemistry

authors： Walker IO

更新日期：1984-11-06 00:00:00

abstract：:Western blot analysis, using a polyclonal antibody to the 240-kDa endogenous inhibitor of the 20 S proteasome, revealed that the inhibitor is a component of the 26 S complex. Although isolated inhibitor displayed a single 40-kDa band on SDS-PAGE, the antibody detected a 55-kDa component in the 26 S proteasome complex....

journal_title：Biochemistry

authors： Li XS,Etlinger JD

更新日期：1992-12-08 00:00:00

abstract：:The N-terminal region of alpha-spectrin is responsible for its association with beta-spectrin in a heterodimer, forming functional tetramers. Non-erythroid alpha-spectrin (alphaII-spectrin) has a significantly higher association affinity for beta-spectrin than the homologous erythroid alpha-spectrin (alphaI-spectrin)....

journal_title：Biochemistry

authors： Li Q,Fung LW

更新日期：2009-01-13 00:00:00

abstract：:Regulation of cellular processes by dietary nutrients is known to affect the likelihood of cancer development. One class of cancer-preventive nutrients, isothiocyanates (ITCs), derived from the consumption of cruciferous vegetables, is known to have various effects on cellular biochemistry. One target of ITCs is macro...

journal_title：Biochemistry

authors： Crichlow GV,Fan C,Keeler C,Hodsdon M,Lolis EJ

更新日期：2012-09-25 00:00:00

abstract：:We describe a reconstitution of light-activated vertebrate photoreceptor GTPase and a purification of the GTP-binding protein (G protein), which is a component of the GTPase and modulates the light-activated phosphodiesterase (PDE) enzyme system. Rod outer segments (ROS) of bull frogs were treated with ethylenediamine...

journal_title：Biochemistry

authors： Shinozawa T,Bitensky MW

更新日期：1981-12-08 00:00:00

abstract：:Extracellular senile plaques composed predominantly of fibrillar amyloid-beta (Abeta) are a major neuropathological feature of Alzheimer's disease (AD). Genetic evidence and in vivo studies suggest that apolipoprotein E (apoE) may contribute to amyloid clearance and/or deposition. In vitro studies demonstrate that nat...

journal_title：Biochemistry

authors： Munson GW,Roher AE,Kuo YM,Gilligan SM,Reardon CA,Getz GS,LaDu MJ

更新日期：2000-12-26 00:00:00

abstract：:Flavin-dependent histone demethylases catalyze the posttranslational oxidative demethylation of mono- and dimethylated lysine residues, producing formaldehyde and hydrogen peroxide in addition to the corresponding demethylated protein. In vivo, histone demethylase LSD1 (KDM1; BCH110) is a component of the multiprotein...

journal_title：Biochemistry

authors： Hwang S,Schmitt AA,Luteran AE,Toone EJ,McCafferty DG

更新日期：2011-02-01 00:00:00

abstract：:Vanadium(IV) oxo-bis(maltolato) (BMOV), an organovanadium compound, is a potent insulinomimetic agent and improves glucose homeostasis in various models of diabetes. We have shown previously that BMOV stimulates the phosphorylation of PKB which may contribute as one of the mechanisms for the insulinomimetic effect of ...

journal_title：Biochemistry

authors： Mehdi MZ,Vardatsikos G,Pandey SK,Srivastava AK

更新日期：2006-09-26 00:00:00

abstract：:Glutamate151 (E151) has been shown to be catalytically essential for the aminopeptidase from Vibrio proteolyticus (AAP). E151 acts as the general acid/base during the catalytic mechanism of peptide hydrolysis. However, a glutamate residue is not the only residue capable of functioning as a general acid/base during cat...

journal_title：Biochemistry

authors： Bzymek KP,Moulin A,Swierczek SI,Ringe D,Petsko GA,Bennett B,Holz RC

更新日期：2005-09-13 00:00:00

abstract：:Pairing rules have been developed to predict the sequence specificity of minor groove binding polyamides containing pyrrole (Py) and imidazole (Im) amino acids. An Im/Py pair distinguishes G.C from C.G and both of these from A.T/T.A base pairs. A Py/Py pair appears not to distinguish A.T from T.A base pairs. To test t...

journal_title：Biochemistry

authors： White S,Baird EE,Dervan PB

更新日期：1996-09-24 00:00:00

abstract：:The dynamics of the backbone and (some of) the side chains of lac headpiece (1-56; lac HP56) have been studied for the free protein and for its complex with lac half-operator DNA by 15N T1 and T1p relaxation measurements combined with [1H-15N] NOE experiments. For the structurally well-defined part of the free lac HP5...

journal_title：Biochemistry

authors： Slijper M,Boelens R,Davis AL,Konings RN,van der Marel GA,van Boom JH,Kaptein R

更新日期：1997-01-07 00:00:00

abstract：:Tetrachlorohydroquinone dehalogenase catalyzes the replacement of chlorine atoms on tetrachlorohydroquinone and trichlorohydroquinone with hydrogen atoms during the biodegradation of pentachlorophenol by Sphingomonas chlorophenolica. The sequence of the active site region of tetrachlorohydroquinone dehalogenase is ver...

journal_title：Biochemistry

authors： Anandarajah K,Kiefer PM Jr,Donohoe BS,Copley SD

更新日期：2000-05-09 00:00:00

abstract：:Following a 1-h [3H]uridine pulse of cells of a human colon carcinoma line, 15% of the radioactivity in heterogeneous nuclear RNA associated with both chromatin and nuclear ribonucleoprotein was not digested to acid soluble fragments during a 2-h incubation with staphylococcal nuclease (EC 3.1.4.7). These [3H]uridine-...

journal_title：Biochemistry

authors： Augenlicht LH,McCormick M,Lipkin M

更新日期：1976-08-24 00:00:00

abstract：:Several unique protein folds that catalyze the hydrolysis of phosphodiester bonds have arisen independently in nature, including the PD(D/E)XK superfamily (typified by type II restriction endonucleases and many recombination and repair enzymes) and the HNH superfamily (found in an equally wide array of enzymes, includ...

journal_title：Biochemistry

authors： Eastberg JH,Eklund J,Monnat R Jr,Stoddard BL

更新日期：2007-06-19 00:00:00

abstract：:An S3 electron paramagnetic resonance (EPR) signal is observed in a variety of photosystem 2 (PS2) samples in which the oxygen-evolving complex (OEC) has been inhibited. These signals have been proposed to be due to an interaction, S2X+, between the manganese cluster in an oxidation state equivalent to S2 and an organ...

journal_title：Biochemistry

authors： MacLachlan DJ,Nugent JH

更新日期：1993-09-21 00:00:00

abstract：:Chemistry, once king of fluorescence microscopy, was usurped by the field of fluorescent proteins. The increased demands of modern microscopy techniques on the "photon budget" require better and brighter fluorophores, causing a renewed interest in synthetic dyes. Here, we review the recent advances in biochemistry, pr...

journal_title：Biochemistry

authors： Lavis LD

更新日期：2017-10-03 00:00:00

abstract：:P-450(15)alpha is a form of cytochrome P-450 purified from liver microsomes of female 129/J mice that is specific for oxidation of testosterone to its 15 alpha-hydroxylated product. Testosterone 15 alpha-hydroxylase activity that was inhibited by anti-P-450(15)alpha antibody was approximately 50 times higher in renal ...

journal_title：Biochemistry

authors： Squires EJ,Negishi M

更新日期：1986-08-26 00:00:00

abstract：:During catalysis by liver alcohol dehydrogenase (ADH), a water bound to the catalytic zinc is replaced by the oxygen of the substrates. The mechanism might involve a pentacoordinated zinc or a double-displacement reaction with participation by a nearby glutamate residue, as suggested by studies of human ADH3, yeast AD...

journal_title：Biochemistry

authors： Plapp BV,Savarimuthu BR,Ferraro DJ,Rubach JK,Brown EN,Ramaswamy S

更新日期：2017-07-18 00:00:00

abstract：:The thylakoid transmembrane DeltapH is the sole energy source driving translocation of precursor proteins by the DeltapH/Tat machinery. Consequently, proton translocation must be coupled to precursor translocation. For the precursor of the 17 kDa protein of the oxygen-evolving complex (pOE17), the protein translocatio...

journal_title：Biochemistry

authors： Musser SM,Theg SM

更新日期：2000-07-18 00:00:00

abstract：:Alditol oxidase (AldO) from Streptomyces coelicolor A3(2) is a soluble monomeric flavin-dependent oxidase that performs selective oxidation of the terminal primary hydroxyl group of several alditols. Here, we report the crystal structure of the recombinant enzyme in its native state and in complex with both six-carbon...

journal_title：Biochemistry

authors： Forneris F,Heuts DP,Delvecchio M,Rovida S,Fraaije MW,Mattevi A

更新日期：2008-01-22 00:00:00

abstract：:In the minor fraction of HDL3 containing alpha-tocopherol (alphaTocOH), selective one-electron oxidation of Trp and Tyr residues of apolipoproteins A-I and A-II by *Br2- radical-anions produces the corresponding semioxidized species, TyrO* and *Trp. Repair of TyrO* by endogenous alphaTocOH generates the alpha-tocopher...

journal_title：Biochemistry

authors： Filipe P,Patterson LK,Bartels DM,Hug GL,Freitas JP,Mazière JC,Santus R,Morlière P

更新日期：2007-12-11 00:00:00