Abstract:
:P-450(15)alpha is a form of cytochrome P-450 purified from liver microsomes of female 129/J mice that is specific for oxidation of testosterone to its 15 alpha-hydroxylated product. Testosterone 15 alpha-hydroxylase activity that was inhibited by anti-P-450(15)alpha antibody was approximately 50 times higher in renal microsomes from 129/J than in BALB/cJ females. Western blots of renal microsomes using anti-P-450(15)alpha antibody showed the presence of immunoreactive protein with a molecular weight identical with that of hepatic P-450(15)alpha in 129/J but not in BALB/cJ female mice. To investigate the genetic basis for the strain differences in this activity, the distribution of P-450(15)alpha-dependent testosterone 15 alpha-hydroxylase activity in renal microsomes from individual females of 129/J and BALB/cJ, of F1 offspring of these strains, and of F1 back-crosses to the progenitor strains were determined. The results were consistent with a sex-related autosomal dominant regulation of the higher activity in 129/J females by a single locus, designated Rsh (regulation of steroid hydroxylase). The amounts of immunochemically cross-reactive P-450(15)alpha protein were linearly correlated with testosterone 15 alpha-hydroxylase activities in renal microsomes from Rsh heterozygotes and homozygotes. At least twice as much mRNA, which hybridized with the cDNA clone for hepatic P-450(15)alpha, was detected in 129/J and 129CF1/J compared to BALB/cJ female kidneys. The evidence suggests a pretranslational regulation of the P-450(15)alpha isozyme in the female mouse kidney by the Rsh locus.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Squires EJ,Negishi Mdoi
10.1021/bi00365a028subject
Has Abstractpub_date
1986-08-26 00:00:00pages
4913-8issue
17eissn
0006-2960issn
1520-4995journal_volume
25pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::For screening a pool of potential substrates that load carrier domains found in nonribosomal peptide synthetases, large molecule mass spectrometry is shown to be a new, unbiased assay. Combining the high resolving power of Fourier transform mass spectrometry with the ability of adenylation domains to select their own ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052333k
更新日期:2006-02-14 00:00:00
abstract::At neutral pH, compound I of Arthromyces ramosus peroxidase (ARP) was stable and was reduced to ferric ARP without apparent formation of compound II upon titration with ascorbate or hydroquinone. In the titration experiments, compound II was seen as an intermediate only at alkaline pH. However, measuring a difference ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00184a038
更新日期:1994-05-10 00:00:00
abstract::Several 3-oxo-4-aza steroids (1) have been identified as inhibitors of the 3 beta-hydroxy-delta 5-steroid dehydrogenase/3-keto-delta 5-steroid isomerase catalyzed conversion of pregnenolone to progesterone. By kinetically decoupling the two enzyme activities isolated from bovine adrenal cortex, it has been demonstrate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00427a021
更新日期:1989-01-10 00:00:00
abstract::The cytochrome bc(1) complex from Paracoccus denitrificans and soluble fragments of its cytochrome c(1) and Rieske ISP subunits are characterized by a combined approach of protein electrochemistry and FTIR difference spectroscopy. The FTIR difference spectra provide information about alterations in the protein upon re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035103j
更新日期:2003-10-28 00:00:00
abstract::In the dark, 4-azido-2-nitrophenyl phosphate (ANPP) is a phosphate analog which behaves like a simple energy-rich phosphate donor for the sarcoplasmic reticulum (SR) Ca-ATPase. Like p-nitrophenyl phosphate (pNPP), ANPP is hydrolyzed by the enzyme only in the presence of calcium and magnesium (K0.5 and Vmax are 0.3 mM ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00175a030
更新日期:1994-03-08 00:00:00
abstract::DNA polymerase delta from calf thymus has been purified to apparent homogeneity by a new procedure which utilizes hydrophobic interaction chromatography with phenyl-Sepharose at an early step to separate most of the calcium-dependent protease activity from DNA polymerase delta and alpha. The purified enzyme migrates a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00304a003
更新日期:1984-04-24 00:00:00
abstract::Myeloperoxidase plays a fundamental role in oxidant production by neutrophils. The enzyme uses hydrogen peroxide to oxidize chloride (Cl-), bromide (Br-), iodide (I-), and the pseudohalide thiocyanate (SCN-) to their respective hypohalous acids. This study for the first time presents transient kinetic measurements of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9818772
更新日期:1998-12-22 00:00:00
abstract::The mammalian plasma vitamin D binding protein (DBP), or Gc-globulin, is recognized to have at least two functional properties: sterol binding and G-actin sequestration. Affinity labeling of the sterol binding site with the radioactive electrophilic ligand, 3 beta-(bromoacetoxy)-25-hydroxycholecalciferol, followed by ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00146a021
更新日期:1992-08-11 00:00:00
abstract::The ubiquitous cyclophilins belong to peptidyl-prolyl cis/trans isomerases (PPIases; EC 5.2.1.8). They are able to catalyze the cis/trans isomerization about peptidyl-prolyl amide bonds. The mode of action of human cytosolic cyclophilin (Cyp18cy) has been studied on substrate analog tetrapeptide-4-nitroanilides contai...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00040a012
更新日期:1995-10-10 00:00:00
abstract::The secretion of lung surfactant in alveolar type II cells is a complex process involving the fusion of lamellar bodies with the plasma membrane. This process is somewhat different from the exocytosis of hormones and neurotransmitters. For example, it is a relatively slower process, and lamellar bodies are very large ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036338y
更新日期:2004-03-30 00:00:00
abstract::We have reexamined the binding properties of the antitumor drug daunomycin using double-helical oligonucleotides 16 base pairs long that were designed to contain preferred binding sites for the drug. The preferred sites are contained in a six base pair core which is flanked on the 5' and 3' ends by tracts of adenines....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00170a011
更新日期:1994-02-01 00:00:00
abstract::When the identity of the metal ion activator, M, is changed within the series, Zn2+, Co2+, Mg2+, Ni2+, Mn2+, and Cd2+, the equilibrium distribution among the central complexes in the phosphoglucomutase system is markedly altered. (The central complexes are Ep-M-Glc-6-P, ED-M-Glc-1,6-P2, and Ep-M-Glc-1-P, where Ep and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00663a016
更新日期:1976-09-07 00:00:00
abstract::The antiviral and antiproliferative activities of type I interferons (IFNs) are mediated by a common receptor, and its second subunit (IFNAR2) exhibits nanomolar affinity to both IFNalpha and IFNbeta subtypes. We have previously determined the structure of the IFN-binding extracellular domain of IFNAR2 (IFNAR2-EC) usi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049606g
更新日期:2004-08-10 00:00:00
abstract::G-protein-coupled receptors (GPCRs) have evolved as highly specialized cellular machinery that can dictate biological outcomes in response to diverse stimuli. Specifically, they induce multiple pathway responses upon structural perturbations induced at local protein sites. GPCRs utilize a concurrent strategy involving...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00884
更新日期:2020-01-28 00:00:00
abstract::In vitro transcription assays have been used to study the rate of ribonucleic acid (RNA) synthesis from the Escherichia coli lactose promotor mutant lacL8UV5 contained on a 203-bp (base pair) restriction fragment. The half-life of long (63-base) RNA production from heparin-resistant RNA polymerase-promotor complexes w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00511a003
更新日期:1981-04-14 00:00:00
abstract::Through the application of a unique method for isolating plasma membranes, it was possible to specifically iodinate cytoplasm-exposed plasma membrane proteins in vegetative cells of the cellular slime mold Dictyostelium discoideum. The original procedure [Chaney, L. K., & Jacobson, B. S. (1983) J. Biol. Chem. 258, 100...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00389a041
更新日期:1987-07-28 00:00:00
abstract::Two thiol-activated endopeptidases with pH optima near pH 7.5 were isolated from the supernatant fraction of rabbit brain homogenates by DEAE-cellulose chromatography, gel filtration and isoelectrofocusing. Peptide bond hydrolysis was measured quantitatively by ion-exchange chromatography with an amino acid analyzer. ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00654a026
更新日期:1976-05-04 00:00:00
abstract::Reconstitution of highly purified aquaporin CHIP (channel-forming integral protein) into proteoliposomes was previously shown to confer high osmotic water permeability (Pf) to the membranes [Zeidel et al. (1992) Biochemistry 31, 7436-7440]. Here we report detailed ultrastructural, pharmacologic, and transport studies ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00172a042
更新日期:1994-02-15 00:00:00
abstract::Extended-spectrum β-lactamases (ESBLs) pose a threat to public health because of their ability to confer resistance to extended-spectrum cephalosporins such as cefotaxime. The CTX-M β-lactamases are the most widespread ESBL enzymes among antibiotic resistant bacteria. Many of the active site residues are conserved bet...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi501195g
更新日期:2015-01-20 00:00:00
abstract::Trypanosoma and Leishmania, pathogens responsible for diseases such as African sleeping sickness, Chagas' heart disease, or Oriental sore, are two of the very few genera that do not use the ubiquitous glutathione/glutathione reductase system to keep a stable cellular redox balance. Instead, they rely on trypanothione ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi963074p
更新日期:1997-05-27 00:00:00
abstract::Ultraviolet absorption (UV) and circular dichroism (CD) spectra of wheat germ 5S RNA, when compared to tRNAPhe, indicate a largely base-paired and base-stacked helical structure, containing up to 36 base pairs. Fourier-transform infrared (FT-IR) spectra of tRNAPhe and wheat germ ribosomal 5S RNA have been acquired at ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00311a012
更新日期:1984-07-31 00:00:00
abstract::Toxic shock syndrome toxin-1 (TSST-1), the potent staphylococcal exoprotein linked to most cases of the toxic shock syndrome, is a V beta-restricted T-cell mitogen (a so-called "superantigen"). TSST-ovine (TSST-O) is a natural variant of TSST-1, and is produced by certain ovine mastitis-associated strains of Staphyloc...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00209a016
更新日期:1994-11-01 00:00:00
abstract::PI-SceI is a member of an unusual class of rare cutting homing endonucleases produced by an autocatalytic protein splicing from a precursor. To analyze the structural and functional domain organization of the endonuclease PI-SceI and to examine whether the DNA binding activity can be structurally separated from the ca...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi980013d
更新日期:1998-06-02 00:00:00
abstract::Methods were developed for isolation of bovine cardiac titin in the apparently full-sized T1 form. The pure protein could be obtained either in the denatured state after chromatography of myofibril extracts on Sephacryl S-1000 or in the native state using hydroxylapatite chromatography. Titin-specific chicken polyclon...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00193a012
更新日期:1994-07-12 00:00:00
abstract::Many proteins that are destined to reside within the lumen of the peroxisome contain the peroxisomal targeting signal-1 (PTS1), a C-terminal tripeptide approximating the consensus sequence -Ser-Lys-Leu-COO(-). The PTS1 is recognized by the tetratricopeptide repeat (TPR) domains of PEX5, a cytosolic receptor that cycle...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi027034z
更新日期:2003-02-18 00:00:00
abstract::The lateral and rotational dynamics of phosphoinositides and their interactions with proteins were characterized using pyrene-labeled lipid analogues. In these systems, the collision frequency of pyrene-labeled lipids was studied by monitoring the monomeric pyrene fluorescence yield as a function of their mole fractio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00028a022
更新日期:1995-07-18 00:00:00
abstract::Pyruvate formate-lyase activating enzyme (PFL-AE) catalyzes the generation of a catalytically essential glycyl radical on pyruvate formate-lyase (PFL). Purified PFL-AE contains an oxygen-sensitive, labile [4Fe-4S] cluster that undergoes cluster interconversions in vitro, with only the [4Fe-4S](+) cluster state being c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9010286
更新日期:2009-10-06 00:00:00
abstract::Cytochrome bc(1), a key enzyme of biological energy conversion, generates or uses a proton motive force through the Q cycle that operates within the two chains of cofactors that embed two catalytic quinone oxidation/reduction sites, the Q(o) site and the Q(i) site. The Q(o) site relies on the joint action of two cofac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801207f
更新日期:2008-11-25 00:00:00
abstract::Transfection of cos-1 cells with either prostaglandin endoperoxide H synthase-1 (PGHS-1) or -2 (PGHS-2) results in a mixed population of cells containing a diverse range of expressed enzyme. The use of fluorescent substrates and antibodies, in conjunction with fluorescence microscopy, provides the means to quantitate ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00021a034
更新日期:1995-05-30 00:00:00
abstract::Fluorescence recovery after photobleaching was used to measure the diffusion coefficient of green fluorescent protein (GFP, 27 kDa) in Escherichia coli in the presence or absence of four coexpressed proteins: cytoplasmic maltose binding protein (42 kDa), tau-40 (45 kDa), alpha-synuclein (14 kDa), or calmodulin (17 kDa...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9004107
更新日期:2009-06-16 00:00:00