Abstract：
:The recA locus of pathogenic mycobacteria differs from that of nonpathogenic species because it contains large intervening sequences nested in the RecA homology region that are excised by an unusual protein-splicing reaction. In vivo assays indicated that Mycobacterium tuberculosis recA partially complemented Escherichia coli recA mutants for recombination and mutagenesis. Further, splicing of the 85 kDa precursor to 38 kDa MtRecA protein was necessary for the display of its activity, in vivo. To gain insights into the molecular basis for partial and lack of complementation by MtRecA and 85 kDa proteins, respectively, we purified both of them to homogeneity. MtRecA protein, but not the 85 kDa form, bound stoichiometrically to single-stranded DNA in the presence of ATP. MtRecA protein was cross-linked to 8-azidoadenosine 5'-triphosphate with reduced efficiency, and kinetic analysis of ATPase activity suggested that it is due to decreased affinity for ATP. In contrast, the 85 kDa form was unable to bind ATP, in the presence or absence of ssDNA and, consequently, was entirely devoid of ATPase activity. Molecular modeling studies suggested that the decreased affinity of MtRecA protein for ATP and the reduced efficiency of its hydrolysis might be due to the widening of the cleft which alters the hydrogen bonds and the contact area between the enzyme and the substrate and changes in the disposition of the amino acid residues around the magnesium ion and the gamma-phosphate. The formation of joint molecules promoted by MtRecA protein was stimulated by SSB when the former was added first. The probability of an association between the lack and partial levels of biological activity of RecA protein(s) to that of illegitimate recombination in pathogenic mycobacteria is considered.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Kumar RA,Vaze MB,Chandra NR,Vijayan M,Muniyappa Kdoi
10.1021/bi9517751subject
Has Abstractpub_date
1996-02-13 00:00:00pages
1793-802issue
6eissn
0006-2960issn
1520-4995pii
bi9517751journal_volume
35pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:We synthesized the 26-residue deoxynucleotide sequence d(TTCCT5GGAATTCCT5GGAA) which folds intramolecularly to form a dumbbell-shaped, double-hairpin structure with a gap between the 3' and the 5' ends. We used T4 polynucleotide kinase to phosphorylate the 5' end followed by T4 DNA ligase to close the 3' and 5' ends. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00427a037
更新日期：1989-01-10 00:00:00
abstract：:Nickel is a fundamental micronutrient for cellular life, but it is toxic in soluble form at nonphysiological concentrations. Such potentially contradictory features required living organisms to develop efficient systems for nickel utilization and homeostasis. This is the case for incorporation of nickel into the activ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi6024676
更新日期：2007-03-20 00:00:00
abstract：:Hydrogenase from Chromatium vinosum contains 1 Ni, 11-12 Fe, and ca. 9 sulfides. EPR and Mössbauer studies of the enzyme prepared in four different oxidation states show that the enzyme contains two Fe4S4 and one Fe3S4 cluster. In the oxidized (2+) state, the Mössbauer parameters of the two Fe4S4 clusters are typical ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00182a029
更新日期：1994-04-26 00:00:00
abstract：:The reaction between chymotrypsin and N-acetyl-L-phenylalanine p-nitroanilide has been studied at subzero temperatures in fluid aqueous dimethyl sulfoxide solvent. Following initiation of the reaction at temperatures as low as -90 degrees C, a series of four reactions prior to the normal rate-limiting step (acylation)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00652a031
更新日期：1976-04-06 00:00:00
abstract：:Microdomains known as "rafts" have been isolated from many cell types as detergent-resistant membranes (DRMs) and are enriched in sphingolipids and cholesterol. However, there has been considerable controversy over whether such domains are found in native membranes or are artificially generated by the purification pro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi026107b
更新日期：2002-11-05 00:00:00
abstract：:In halophilic archaea the photophobic response is mediated by the membrane-embedded 2:2 photoreceptor/-transducer complex SRII/HtrII, the latter being homologous to the bacterial chemoreceptors. Both systems bias the rotation direction of the flagellar motor via a two-component system coupled to an extended cytoplasmi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501160q
更新日期：2015-01-20 00:00:00
abstract：:There are at least nine neurodegenerative diseases associated with proteins that contain an unusually expanded polyglutamine domain, the best known of which is Huntington's disease. In all of these diseases, the mutant protein aggregates into neuronal inclusions; it is generally, although not universally, believed tha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700806c
更新日期：2007-11-06 00:00:00
abstract：:Drug molecules are typically hydrophobic and small in order to traverse membranes to reach cytoplasmic targets, but we have discovered that more polar molecules can be delivered across membranes using water-soluble, moderately hydrophobic membrane peptides of the pHLIP (pH low insertion peptide) family. Delivery of po...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2009773
更新日期：2011-11-29 00:00:00
abstract：:Using heteronuclear NMR spectroscopy, we demonstrate that a 13-residue peptide (MS-QIKRLLSEKKT) from the cytoplasmic tail of CD4 binds to Nef protein. This part of CD4 is critical for downregulation of CD4 by HIV-1 Nef [Aiken et al. (1994) Cell 76, 853-864]. We show that a control peptide without the central dileucine...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9611164
更新日期：1996-08-13 00:00:00
abstract：:Glycogen synthase kinase 3β (GSK-3β) is a serine-threonine kinase belonging to the CMGC family that plays a key role in many biological processes, such as glucose metabolism, cell cycle regulation, and proliferation. Like most protein kinases, GSK-3β is regulated via multiple pathways and sites. We performed all-atom ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100822q
更新日期：2010-12-28 00:00:00
abstract：:Binding of 125I-labeled human alpha-thrombin to endothelial cells derived from bovine corneas was studied in tissue culture. Specific and saturable binding to the cell surface occurred at 37 degrees C but to a much smaller extent at 4 degrees C. Binding of [125I]thrombin to a specific site on these cells with formatio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00505a027
更新日期：1981-01-20 00:00:00
abstract：:Acyclovir triphosphate, ganciclovir triphosphate and penciclovir triphosphate inhibited DNA polymerases alpha, delta, and epsilon. Each triphosphate preferentially inhibited pol delta, although ganciclovir triphosphate was the most impressive of the three; the Ki for inhibition of pol delta was 2 microM (competitive w...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00008a014
更新日期：1995-02-28 00:00:00
abstract：:The flavoenzyme tryptophan 2-monooxygenase catalyzes the oxidation of tryptophan to indoleacetamide, carbon dioxide, and water. The enzyme is a homologue of l-amino acid oxidase. In the structure of l-amino acid oxidase complexed with aminobenzoate, Tyr372 hydrogen bonds with the carboxylate of the inhibitor in the ac...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035300i
更新日期：2003-12-02 00:00:00
abstract：:The interaction between the calcium binding and inhibitory components of troponin is central to the regulation of muscle contraction. In this work, two-dimensional heteronuclear single-quantum coherence nuclear magnetic resonance (2D-¿1H,15N¿-HSQC NMR) spectroscopy was used to determine the stoichiometry, affinity, an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992579n
更新日期：2000-03-21 00:00:00
abstract：:The central importance of chorismate enzymes in bacteria, fungi, parasites, and plants combined with their absence in mammals makes them attractive targets for antimicrobials and herbicides. Two of these enzymes, anthranilate synthase (AS) and aminodeoxychorismate synthase (ADCS), are structurally and mechanistically ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00013
更新日期：2015-04-14 00:00:00
abstract：:The solution structure of the 63-residue heregulin-alpha (HRG-alpha) epidermal growth factor (EGF)-like domain, corresponding to residues 177-239 of HRG-alpha, has been determined to high resolution using data from two-dimensional and three-dimensional homo- and heteronuclear NMR spectroscopy. The structure is based o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952626l
更新日期：1996-03-19 00:00:00
abstract：:A full characterization of the thermodynamic forces underlying ligand-associated conformational changes in proteins is essential for understanding and manipulating diverse biological processes, including transport, signaling, and enzymatic activity. Recent experiments on the maltose binding protein (MBP) have provided...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201481a
更新日期：2011-12-06 00:00:00
abstract：:(Z)-3-(Fluoromethyl)phosphoenolpyruvate has been synthesized in nine chemical steps from glyoxylic acid. The compound is stable at pH 3, but at pH 8 it decomposes within seconds to give 2-oxo-3-butenoate. When 3-(fluoromethyl)phosphoenolpyruvate is added to a solution of phosphoenolpyruvate carboxylase or pyruvate kin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00404a039
更新日期：1988-02-23 00:00:00
abstract：:We have prepared the following cadmium-113-substituted derivatives of bovine superoxide dismutase and recorded the nuclear magnetic resonance (NMR) spectrum of the cadmium: 2Cd(II), in which Cd(II) is presumed to bind to the Zn(II) site and the copper site is unoccupied, and 2Cd(II)--2Cu(I), which is analogous to the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00544a031
更新日期：1980-02-05 00:00:00
abstract：:Malondialdehyde (MDA) and nucleobase propenals can transfer oxopropenyl groups to guanine residues of DNA to yield pyrimodopurinone (M(1)G) adducts. The DNA structural requirements for reaction with alpha,beta-unsaturated aldehydes were explored. We found that single-stranded DNA is more sensitive to oxopropenylation ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0113059
更新日期：2002-04-16 00:00:00
abstract：:Rod outer segment membrane guanylate cyclase (ROS-GC) transduction system is a central component of the Ca(2+)-sensitive phototransduction machinery. The system is composed of two parts: Ca(2+) sensor guanylate cyclase activating protein (GCAP) and ROS-GC. GCAP senses Ca(2+) impulses and inhibits the cyclase. This ope...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034025x
更新日期：2003-05-20 00:00:00
abstract：:Active trihydroxynaphthalene reductase (3HNR) is essential for the biosynthesis of fungal melanin by Magnaporthe grisea and is a focus of inhibitor design studies directed toward control of blast disease in rice. Tricyclazole, a preventative fungicide against rice blast, has been previously characterized as inhibiting...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962355u
更新日期：1997-02-18 00:00:00
abstract：:The contribution of ligand dynamics to CYP allosterism has not been considered in detail. On the basis of a previous study, we hypothesized that CYP2A6 and CYP2E1 accommodate multiple xylene ligands. As a result, the intramolecular ( k H/ k D) obs values observed for some xylene isomers are expected to be dependent on...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi702020y
更新日期：2008-03-04 00:00:00
abstract：:The effects of solution variables on the in vitro reconstitution of calf brain tubulin, purified by the method of Weisenberg et al. (Weisenberg, R. C., Borisy, G. G., and Taylor, E. W. (1968), Biochemistry 7, 4466-4479; Weisenberg, R. C., and Timasheff, S. N. (1970), Biochemistry 9, 4110-4116), as modified by Lee et a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00627a037
更新日期：1977-04-19 00:00:00
abstract：:The Raman spectra of the nonbridging V--O bonds in the myosin S1.MgADP.Vi complex, often believed to be a transition-state analogue for the phosphotransfer reaction catalyzed by myosin, and in a vanadate solution model compound have been obtained using Raman difference spectroscopic techniques. A symmetric/asymmetric ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980556n
更新日期：1998-08-04 00:00:00
abstract：:Telomeres terminate nearly exclusively in single-stranded DNA (ssDNA) overhangs comprised of the G-rich 3' end. This overhang varies widely in length from species to species, ranging from just a few bases to several hundred nucleotides. These overhangs are not merely a remnant of DNA replication but rather are the res...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00496
更新日期：2016-09-27 00:00:00
abstract：:The ATP binding cassette (ABC) transporters ABCG2 and ABCB1 perform ATP hydrolysis-dependent efflux of structurally highly diverse compounds, collectively called allocrites. Whereas much is known about allocrite-ABCB1 interactions, the chemical nature and strength of ABCG2-allocrite interactions have not yet been asse...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00649
更新日期：2015-10-13 00:00:00
abstract：:Photosynthetic water oxidation takes place at the Mn4CaO5 cluster in photosystem II. Around the Mn4CaO5 cluster, a hydrogen bond network is formed by several water molecules, including four water ligands. To clarify the role of this water network in the mechanism of water oxidation, we investigated the effects of the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b01120
更新日期：2016-01-26 00:00:00
abstract：:X-ray absorption spectra are reported for the multi-Cu oxidase Rhus vernicifera laccase in oxidized and fully reduced forms and for laccase from which the type 2 Cu has been depleted (T2D). The structure of the Cu K edge for both preparations shows the presence of CuII and CuI in the oxidized and reduced states, respe...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00310a008
更新日期：1984-07-17 00:00:00
abstract：:The cytidine repressor (CytR) is a member of the LacR family of bacterial repressors with distinct functional features. The Escherichia coli CytR regulon comprises nine operons whose palindromic operators vary in both sequence and, most significantly, spacing between the recognition half-sites. This suggests a strong ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200205v
更新日期：2011-08-09 00:00:00
